breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 246,747 G→T S12S (TCG→TCT yafL → NlpC/P60 family protein YafL
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 313,543 G→A intergenic (‑301/‑1748) ykgR ← / → insE1 putative membrane protein YkgR/IS3 element protein InsE
RA 339,935 C→T A56V (GCC→GTC)  yahH → putative uncharacterized protein YahH
RA 339,941 C→T T58I (ACT→ATT)  yahH → putative uncharacterized protein YahH
RA 339,958 G→A D64N (GAC→AAC)  yahH → putative uncharacterized protein YahH
RA 339,979 G→A V71M (GTG→ATG)  yahH → putative uncharacterized protein YahH
RA 339,986 G→A R73Q (CGA→CAA)  yahH → putative uncharacterized protein YahH
JC JC 458,790 IS186 (–) +6 bp :: Δ1 bp intergenic (+90/‑93) clpX → / → lon ATP‑dependent Clp protease ATP‑binding subunit ClpX/Lon protease
RA 480,295 2 bp→AA coding (13‑14/219 nt) hha ← hemolysin expression‑modulating protein Hha
RA 535,582 A→T E556V (GAG→GTG)  gcl → glyoxylate carboligase
RA 537,531 C→T intergenic (+67/‑102) glxR → / → ybbW tartronate semialdehyde reductase 2/putative allantoin transporter
RA 543,263 2 bp→GT coding (783‑784/786 nt) allE ← (S)‑ureidoglycine aminohydrolase
RA 554,493 G→A intergenic (‑56/‑118) ppiB ← / → cysS peptidyl‑prolyl cis‑trans isomerase B/cysteine‑‑tRNA ligase
RA 628,569 A→G N7D (AAT→GAT)  entA → 2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase
RA 659,517 G→A L234L (CTG→TTG)  lipA ← lipoyl synthase
RA 659,527 G→T T230T (ACC→ACA lipA ← lipoyl synthase
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 727,761 C→T E324E (GAG→GAA kdpA ← K(+) transporting P‑type ATPase subunit KdpA
RA 809,217 2 bp→AG coding (40‑41/1290 nt) bioA ← adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
RA 905,659 C→T F249F (TTC→TTT amiD → N‑acetylmuramoyl‑L‑alanine amidase D
RA 907,652 C→T E211K (GAG→AAG)  ybjT ← putative NAD(P)‑binding protein YbjT
RA 909,094 G→A L67L (CTC→CTT ltaE ← low‑specificity L‑threonine aldolase
RA 973,577 C→T S14F (TCC→TTC)  cmoM → tRNA cmo(5)U34 methyltransferase
RA 1,005,691 T→A I308I (ATT→ATA pyrD → dihydroorotate dehydrogenase, type 2
RA 1,005,703 C→T A312A (GCC→GCT pyrD → dihydroorotate dehydrogenase, type 2
RA 1,043,607 G→A L202L (CTG→TTG)  etk ← protein‑tyrosine kinase Etk
RA 1,050,092 C→T S87L (TCA→TTA) 
L22L (CTC→CTT
insA4 →
insB4 →
IS1 family protein InsA
IS1 family protein InsB
RA 1,146,079 C→A V173F (GTT→TTT)  yceF ← m(7)GTP pyrophosphatase
RA 1,157,902 G→T V12F (GTC→TTC)  ptsG → glucose‑specific PTS enzyme IIBC component
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
JC JC 1,185,790 IS2 (–) +5 bp intergenic (‑196/‑50) potA ← / → pepT spermidine preferential ABC transporter ATP binding subunit/peptidase T
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,304 G→A E394E (GAG→GAA icd → isocitrate dehydrogenase
RA 1,196,316 T→A D398E (GAT→GAA icd → isocitrate dehydrogenase
RA 1,220,493 C→T intergenic (+1292/+1812) ymgF → / ← ymgD inner membrane protein YmgF/PF16456 family protein YmgD
RA 1,233,669 G→A S350F (TCT→TTT)  nhaB ← Na(+):H(+) antiporter NhaB
RA 1,290,947 C→T Q236* (CAA→TAA)  rssB → regulator of RpoS
RA 1,292,992 A→C intergenic (‑70/‑535) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,374,498 G→T G137C (GGC→TGC)  ycjP → putative ABC transporter membrane subunit YcjP
RA 1,405,758 G→A intergenic (‑7/‑221) mcaS ← / → smrA small regulatory RNA McaS/DNA endonuclease SmrA
JC JC 1,419,427 IS1 (–) +9 bp coding (22‑30/135 nt) ydaG ← uncharacterized protein YdaG
RA 1,425,329 Δ1 bp intergenic (+90/‑48) trkG → / → ynaK K(+) transporter TrkG/ParB‑like nuclease domain‑containing protein YnaK
RA 1,433,144 G→A F177F (TTC→TTT pinR ← putative site‑specific recombinase
RA 1,531,689 A→G intergenic (‑1185/‑127) yncO ← / → ydcC protein YncO/H repeat‑associated putative transposase YdcC
RA 1,553,630 C→A G70V (GGT→GTT)  adhP ← ethanol dehydrogenase/alcohol dehydrogenase
RA 1,555,731 Δ1 bp intergenic (‑62/+95) maeA ← / ← sra malate dehydrogenase (oxaloacetate‑decarboxylating)/ribosome‑associated protein Sra
RA 1,600,234 C→T intergenic (‑25/+54) lsrK ← / ← lsrR autoinducer‑2 kinase/DNA‑binding transcriptional repressor LsrR
RA 1,601,098 C→A S48S (TCG→TCT lsrR ← DNA‑binding transcriptional repressor LsrR
RA 1,601,103 C→T V47M (GTG→ATG)  lsrR ← DNA‑binding transcriptional repressor LsrR
RA 1,632,712 A→G intergenic (‑427/‑178) ydfJ ← / → ynfT putative transporter YdfJ/protein YnfT
RA 1,641,002 T→C intergenic (+27/+337) ynfU → / ← cspB putative zinc‑binding protein YnfU/cold shock‑like protein CspB
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,649,971 C→T P60S (CCG→TCG)  ydfD → lysis protein
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,682,694 A→T Q179L (CAA→CTA)  rstA → DNA‑binding transcriptional regulator RstA
RA 1,702,757 C→T F175F (TTC→TTT add → adenosine deaminase
RA 1,776,715 A→T S377C (AGT→TGT)  ydiF → putative acetate‑CoA transferase YdiF
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
JC JC 1,907,395 IS1 (–) +9 bp coding (33‑41/210 nt) cspC ← transcription antiterminator and regulator of mRNA stability CspC
RA 1,909,387 A→T L238* (TTA→TAA)  kdgR ← DNA‑binding transcriptional repressor KdgR
RA 1,987,499 C→A intergenic (+71/+8) ftnB → / ← yecJ putative ferritin‑like protein/DUF2766 domain‑containing protein YecJ
JC 2,014,041 (TACGGCGCAGCTGGACTTCGCCAGT)1→2 coding (813/1659 nt) fliF → flagellar basal‑body MS‑ring and collar protein
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,117,201 C→T E402K (GAA→AAA)  wcaK ← colanic acid biosynthesis pyruvyl transferase WcaK
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,239,943 T→A K136* (AAA→TAA)  mglB ← D‑galactose/methyl‑galactoside ABC transporter periplasmic binding protein
RA 2,330,263 A→G intergenic (‑466/+4073) yfaQ ← / ← yfaT tandem DUF2300 domain‑containing protein YfaQ/DUF1175 domain‑containing protein YfaT
RA 2,384,808 C→T K305K (AAG→AAA yfbK ← IPR002035/DUF3520 domain‑containing protein YfbK
RA 2,410,419 G→A L312L (CTG→TTG)  yfbS ← putative transporter YfbS
RA 2,484,827 C→T L152F (CTT→TTT)  evgS → sensor histidine kinase EvgS
RA 2,674,147 T→A Q181L (CAG→CTG)  yphB ← putative aldose 1‑epimerase YphB
RA 2,848,629 A→T F205I (TTT→ATT)  hycC ← formate hydrogenlyase subunit HycC
RA 2,867,551 T→G M1M (ATG→CTG) † rpoS ← RNA polymerase sigma factor RpoS
JC JC 2,913,392 IS2 (–) +5 bp coding (256‑260/2235 nt) relA ← GDP/GTP pyrophosphokinase
RA 2,941,904 G→A H222Y (CAT→TAT)  gcvA ← DNA‑binding transcriptional dual regulator GcvA
RA 3,088,010 2 bp→TT intergenic (+150/‑273) metK → / → galP methionine adenosyltransferase/galactose:H(+) symporter
RA 3,218,844 G→A P78L (CCC→CTC)  aer ← aerotaxis receptor
RA 3,309,321 A→G T616T (ACT→ACC pnp ← polynucleotide phosphorylase
RA 3,336,411 2 bp→AT coding (83‑84/1260 nt) murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,432,105 C→T L120L (CTG→CTA smg ← DUF494 domain‑containing protein Smg
RA 3,443,961 G→A A46V (GCC→GTC)  secY ← Sec translocon subunit SecY
RA 3,443,980 G→A P40S (CCG→TCG)  secY ← Sec translocon subunit SecY
RA 3,447,871 +C coding (279/372 nt) rplN ← 50S ribosomal subunit protein L14
RA 3,452,022 G→A P89S (CCG→TCG)  rplD ← 50S ribosomal subunit protein L4
RA 3,452,218 G→A F23F (TTC→TTT rplD ← 50S ribosomal subunit protein L4
RA 3,457,378 C→T L334L (CTC→CTT gspD → Type II secretion system protein GspD
RA 3,458,245 A→C S623S (TCA→TCC gspD → Type II secretion system protein GspD
RA 3,474,425 T→G K43T (AAA→ACA)  rpsL ← 30S ribosomal subunit protein S12
RA 3,474,539 T→C N5S (AAC→AGC)  rpsL ← 30S ribosomal subunit protein S12
JC JC 3,560,081 IS1 (+) +9 bp intergenic (+215/+533) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,560,095 C→T intergenic (+229/+527) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,686,705 G→A F823F (TTC→TTT bcsC ← cellulose synthase outer membrane channel
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,712,407 G→A G176G (GGC→GGT yhjY ← putative outer membrane protein YhjY
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,729,955 2 bp→AA coding (810‑811/1323 nt) xylA ← xylose isomerase
JC JC 3,731,420 IS1 (–) +9 bp coding (290‑298/993 nt) xylF → xylose ABC transporter periplasmic binding protein
RA 3,769,447 G→A E35K (GAA→AAA)  yibV → PF15596 family protein YibV
RA 3,773,040 (G)6→4 coding (760‑761/1914 nt) mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,783,983 G→A S49F (TCC→TTC)  secB ← protein export chaperone SecB
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,865,885 A→G V130A (GTT→GCT)  yidE ← putative transport protein YidE
RA 3,899,195 C→T E67K (GAA→AAA)  yieK ← putative glucosamine‑6‑phosphate deaminase YieK
RA 3,903,234 G→A V156V (GTC→GTT bglB ← 6‑phospho‑beta‑glucosidase B
RA 3,961,662 G→T G329V (GGC→GTC)  rep → ATP‑dependent DNA helicase Rep
RA 3,965,174 G→A H153Y (CAC→TAC)  rhlB ← ATP‑dependent RNA helicase RhlB
RA 3,968,683 G→A M256I (ATG→ATA rfe → UDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase
RA 3,969,748 T→C S240P (TCG→CCG)  wzzE → enterobacterial common antigen polysaccharide co‑polymerase
RA 3,999,664 C→T S561L (TCG→TTG)  uvrD → DNA helicase II
RA 4,009,698 G→A A177T (GCA→ACA)  pldB → lysophospholipase L2
RA 4,017,197 C→T intergenic (+5/‑136) udp → / → rmuC uridine phosphorylase/putative recombination limiting protein RmuC
RA 4,084,903 T→G N307T (AAC→ACC)  fdoG ← formate dehydrogenase O subunit alpha
RA 4,086,300 A→C E95D (GAA→GAC fdhD → sulfurtransferase for molybdenum cofactor sulfuration
RA 4,092,703 A→G F41L (TTC→CTC)  frvA ← putative PTS enzyme IIA component FrvA
RA 4,105,809 G→A intergenic (‑139/‑11) cpxR ← / → cpxP DNA‑binding transcriptional dual regulator CpxR/periplasmic protein CpxP
RA 4,108,758 T→C intergenic (+244/‑76) pfkA → / → sbp 6‑phosphofructokinase 1/sulfate/thiosulfate ABC transporter periplasmic binding protein Sbp
RA 4,113,851 G→A H208Y (CAT→TAT)  fpr ← flavodoxin/ferredoxin‑NADP(+) reductase
RA 4,143,377 C→T S283L (TCA→TTA)  frwC → putative PTS enzyme IIC component FrwC
RA 4,156,839 G→A intergenic (+50/‑11) argB → / → argH acetylglutamate kinase/argininosuccinate lyase
RA 4,157,116 Δ1 bp coding (267/1374 nt) argH → argininosuccinate lyase
RA 4,193,996 T→G D70A (GAT→GCT)  thiE ← thiamine phosphate synthase
RA 4,202,259 G→A G112R (GGG→AGG)  zraS → sensor histidine kinase ZraS
RA 4,215,347 T→A intergenic (+138/‑131) metA → / → aceB homoserine O‑succinyltransferase/malate synthase A
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,293,781 2 bp→AT coding (239‑240/597 nt) nrfG → putative formate‑dependent nitrite reductase complex subunit NrfG
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,402,046 G→A W3* (TGG→TGA hflK → regulator of FtsH protease
RA 4,426,760 G→A I221I (ATC→ATT yjfZ ← DUF2686 domain‑containing protein YjfZ
RA 4,449,586 G→A P23S (CCG→TCG)  ppa ← inorganic pyrophosphatase
RA 4,452,714 G→A Q48Q (CAG→CAA ytfT → galactofuranose ABC transporter putative membrane subunit YtfT
RA 4,462,459 G→A Q68* (CAG→TAG)  nrdD ← anaerobic ribonucleoside‑triphosphate reductase
RA 4,473,900 C→T G52R (GGA→AGA)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,475,493 T→C F19F (TTT→TTC yjgL → protein YjgL
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,546,601 C→T I502I (ATC→ATT fimD → type I fimbriae usher protein
RA 4,585,480 G→A Q428* (CAA→TAA)  hsdR ← type I restriction enzyme EcoKI endonuclease subunit
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 576631 577298 668 4 [3] [3] 4 [nmpC] [nmpC]
* * ÷ NC_000913 578349 579183 835 4 [3] [0] 31 [rzpD]–borD [rzpD],[rzoD],borD
* * ÷ NC_000913 791603 792872 1270 23 [0] [2] 36 [galE]–[modF] [galE],[modF]
* * ÷ NC_000913 1094453–1095500 1106534 11035–12082 4 [3] [3] 4 [insF4]–[clsC] [insF4],insE4,ycdU,serX,ghrA,ycdX,ycdY,ycdZ,csgG,csgF,csgE,csgD,csgB,csgA,csgC,ymdA,ymdB,[clsC]
* * ÷ NC_000913 1106618 1107614 997 4 [2] [0] 17 clsC cardiolipin synthase C
* * ÷ NC_000913 1196351 1211423 15073 4 [3] [3] 4 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1632774 1633045–1632936 163–272 4 [3] [3] 4 ynfT ynfT
* * ÷ NC_000913 1978495 1979270–1978503 9–776 22 [0] [0] 18 insB5–insA5 insB5,insA5
* * ÷ NC_000913 2151576 2151676 101 4 [2] [2] 4 [yegJ] [yegJ]
* * ÷ NC_000913 3423878–3424438 3424438 1–561 4 [3] [3] 4 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 257908 =NA (NA)34 (1.170) 31/484 0.1 94.4% noncoding (768/768 nt) IS1 repeat region
?NC_000913 792873 = 2 (0.070)coding (916/1473 nt) modF ABC family protein ModF
* ? NC_000913 = 258675NA (NA)23 (0.790) 22/484 0.3 100% noncoding (1/768 nt) IS1 repeat region
?NC_000913 = 791602 0 (0.000)coding (454/1017 nt) galE UDP‑glucose 4‑epimerase
* ? NC_000913 = 275149NA (NA)31 (1.070) 25/484 0.2 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 579184 = 0 (0.000)coding (411/411 nt) ybcV DUF1398 domain‑containing protein YbcV
* ? NC_000913 315229 =NA (NA)17 (0.590)
+TCA
16/478 0.7 100% noncoding (1/1255 nt) IS3 repeat region
?NC_000913 1107615 = 0 (0.000)coding (1261/1422 nt) clsC cardiolipin synthase C
* ? NC_000913 = 12994980 (0.000)23 (0.840) 20/460 0.4 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 = 1300693 NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 1299499 =NA (NA)17 (0.620) 17/460 0.6 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 1300694 = 0 (0.000)intergenic (+147/‑488) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1397238NA (NA)22 (0.760) 20/484 0.4 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1979271 =0 (0.000)18 (0.620) 17/484 0.6 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL
* ? NC_000913 = 454269014 (0.480)5 (0.180) 5/466 2.3 25.3% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 16 (0.570)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit