Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 905,659 C→T F249F (TTC→TTT amiD → N‑acetylmuramoyl‑L‑alanine amidase D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913905,6590CT95.7% 60.3 / ‑3.5 23F249F (TTC→TTTamiDN‑acetylmuramoyl‑L‑alanine amidase D
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (14/8);  total (15/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.61e-01

CAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGC  >  NC_000913/905426‑905902
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    
cAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTTCGCCCGACGTtatata                                                                                                                                                                                                                                      >  1:203072/1‑250 (MQ=255)
 ggcgcAAAGACGATCCTGGGCCTTGATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATGGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGTTATATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACCCCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTTCGCCCGACGTTATATAAc                                                                                                                                                                                                                                    <  2:270286/250‑1 (MQ=255)
                                      gcaACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCAGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAACCCTGATATGACACCGCGCGAGCAGCGGCGCGTGCTTATGGCATTCCAGATGCATTTTCGCCCGCCTTTATATAACGGCGAGGAGGATGCAGAACCTCAGGCGATTGCCgc                                                                                                                                                                                                 >  2:108839/1‑248 (MQ=255)
                                        aaCTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTTCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGc                                                                                                                                                                                              >  1:288344/1‑250 (MQ=255)
                                                                       ggATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTTCGCCCGCCGTTATATAACGGCGAAGCGGATGCAGAAACTCAGCCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGcgcg                                                                                                                                                              >  1:38253/1‑251 (MQ=255)
                                                                        gATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTTCGCCCGACGTTATATAACGGCGAAGCGGATGAAGAAACTAAGGCGATTGCCGCAGCATTACTGGAGAACTACGGGCAGGATTAGCGCgg                                                                                                                                                             >  1:337129/1‑251 (MQ=255)
                                                                                  gggTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATCTGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTTCGCCCGACGTTATCTAACGGAGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCGTTGCTGGAGACATACGGGCAGGATTAGCCAGGCCGTTTTCCg                                                                                                                                                   >  2:106419/1‑251 (MQ=255)
                                                                                                cTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTTCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGCAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGccc                                                                                                                                     >  2:300493/1‑250 (MQ=255)
                                                                                                    ccGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTTCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAggcg                                                                                                                                 >  1:33277/1‑251 (MQ=255)
                                                                                                                             aGATACCGCGTCATTGCTGGAGCTTTTGGCGCGATACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTTCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGAAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTtcctccc                                                                                                        >  1:61110/1‑250 (MQ=255)
                                                                                                                                 aCCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTTCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGt                                                                                                    <  1:300493/251‑1 (MQ=255)
                                                                                                                                  ccgtgTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTTCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGCAGCATTGCTGGAGAAATACGGGAAGGATTAGCGCAGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGTTACCTTCaggtggga                                                                                                     >  2:165350/5‑242 (MQ=255)
                                                                                                                                  ccgtgTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTTCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTc                                                                                                             <  1:165350/237‑1 (MQ=255)
                                                                                                                                     cgTCATTGCTGGGGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATAGGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTTCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgatg                                                                                                <  2:203072/251‑1 (MQ=255)
                                                                                                                                      gTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTTCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGtgatga                                                                                               <  1:134012/251‑1 (MQ=255)
                                                                                                                                                               tACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTTCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCGGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGATTTCTCGATACCTTCATCCAGGGCGATGACCGGCTGATAACCTAACTCTTCCTg                                                                      >  1:129979/1‑251 (MQ=255)
                                                                                                                                                                       tGACGTTAAACCTGATATGACAACGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGACACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCCGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACATAACTCTTCCTGCGCAcgcg                                                              >  2:133211/1‑251 (MQ=255)
                                                                                                                                                                        gactttaaacctaaattGACACCGCGCGAGCGGCGGTGCGGTATTAGTGCATTCCAGATGCATTTTCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAcgcg                                                              <  2:263407/234‑1 (MQ=255)
                                                                                                                                                                                            cccgctggAGCGGCGGCGCGTGATTATGGCATTCCAGATGCATTCTCGCTCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCTATTGCCGAAGCATTGCTGGAGAAATACGGGCAGTATTAGCGCGGCAGTTTTCCGTTGTCGCGTAGCCAGGCGGCAGTTTTCGCGATACCTTCATCCAGTGTGATGATCGGCTGAGAACCTAACACTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCaa                                        <  1:159980/244‑1 (MQ=255)
                                                                                                                                                                                                          ggcgCGTGATTATGGCATTCCAGATGCATTTTCGCCCGACGTTATATAAAGGAGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGGCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCCTCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCTCACGCGTAATATCCAGAGTAAAGGCCAAATTCAACCTgg                            >  2:165416/1‑249 (MQ=255)
                                                                                                                                                                                                                  aTTATGGCATTCCAGATGCATTTTCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGtt                  >  1:118194/1‑250 (MQ=255)
                                                                                                                                                                                                                    tATGGCATGCCAGATGCATTTTCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAg                 <  1:320964/250‑1 (MQ=255)
                                                                                                                                                                                                                                  tGCATTTTCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGGAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCGTCCAGGGTGAGGACAGACTGATAACTTAACTAATCCTGCCCACGCGTAATATACCGCGTAAGAGCAAAATTCAACTTTGATAAGCCGAAGTGGTTCAGAggcggc  >  2:256237/1‑251 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    
CAGCGCAAAGACGATCCGGGGCCATTATTTCCCTGGCAGCAACTGGCGCAGCAGGGGATTGGTGCCTGGCCGGATGCGCAGCGGGTTAACTTTTACCTTGCCGGGCGCGCGCCGCACACACCGGTAGATACCGCGTCATTGCTGGAGCTTTTGGCGCGCTACGGTTATGACGTTAAACCTGATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGC  >  NC_000913/905426‑905902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: