Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	246,747	G→T	S12S (TCG→TCT)	yafL →	NlpC/P60 family protein YafL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	246,747	0	G	T	100.0%	72.6 / NA	26	S12S (TCG→TCT)	yafL	NlpC/P60 family protein YafL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/11);  total (15/11)

CATTGGGGTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTA‑AGT‑TTGTT‑CAGAAACGATCC‑GGGATACAC‑TGCAACATTAAAGCATGACCA‑GCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCTCCGCTC  >  NC_000913/246500‑246990                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    cATTGGGGTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTA‑AGT‑TTGTT‑CAGAAACGATCC‑GGGATACAC‑TGCAACATTAAAGCATGACCA‑GCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGa                                                                                                                                                                                                                                                  <  2:50912/251‑1 (MQ=255)                                                    atgtaGCAATAGCGACAATCCATGAGTAATTTAATAAACGGATTTTTTTACATAATTAACTGAAATATAGTGGTTATTTTTATAATAATGGTTTAGTA‑AGT‑TTGTT‑CAAAAACGTGCC‑GGGATACAC‑TGCAACATTAAAGCATGACCT‑GCCATTATTGAGTATGTCCTTGTCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCAGTTGCCAGCGCCACcacatca                                                                                                                                                                                               <  2:217798/247‑1 (MQ=255)                                                         gCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTA‑AGT‑TTGTT‑CAGAAACGATCC‑GGGATACAC‑TGCAACATTAAAGCATGACCA‑GCCATTATTGAGTATGTCCTTGCCGTCGCTTCCTTCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACttat                                                                                                                                                                                          >  2:12259/1‑251 (MQ=255)                                                                cGACAATCCATGAGTACTTTAATAAAAGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTA‑AGT‑TTGTT‑CAGAAACGATCC‑GGGATACAC‑TGCAACATTAAAGCATGACCA‑GCCATTATTGTGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACTTAACATATCTCTTTc                                                                                                                                                                                   >  2:218659/1‑251 (MQ=255)                                                                          tGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTA‑AGT‑TTGTT‑CAGAAACGATCC‑GGGATACAC‑TGCAACATTAAAGCATGACCA‑GCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTAcgccg                                                                                                                                                                         >  1:346828/1‑251 (MQ=255)                                                                                      aaaaCGGTTGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTA‑ATT‑TTGTT‑CAGAAACGATCC‑GGGATACAC‑TGCAACATTAAATCATGCCCA‑GCCATTATTGAGGATGTCCTGGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTTTTCATTTTCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGa                                                                                                                                                             <  1:348943/250‑1 (MQ=255)                                                                                         aCGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTA‑AGT‑TTGTT‑CAGAACCGATCC‑GGGATACAC‑TGCAACATTAAAGAATGACCA‑GACATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGc                                                                                                                                                          >  1:29215/1‑251 (MQ=255)                                                                                                gttttACATAATTATCGAAAATATATAT‑TTTATTTTAAAAATATTGTTT‑TTATAGTGTTGTT‑CAGAAAGGATCC‑GGCTTACAC‑TGCACTACAAAAG‑AAGCCCAAGCCATTATTGATTCTGGCCTTGCCGTTTATTCCGTTCTTTGTATTGTCTGGATTACTTTTGATTTGTTTGCCTTTTCCTTCATTTGCCAGCGGCACCCCATCACATATCTCTTGCAGCTACGCCGCCCGCCAGCGGATGCAAAAcc                                                                                                                                                    <  2:73963/249‑1 (MQ=255)                                                                                                           ttAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTA‑AGT‑TTGTT‑CAGAAACGATCC‑GGGATACAC‑TGCAACATTAAAGCATGACCA‑GCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTttat                                                                                                                                        >  1:156646/1‑251 (MQ=255)                                                                                                             aaattaaaTATATATGTTAATTTTAAAAAAATGGTTAATTA‑ATT‑TTGTT‑TAAAAATG‑TCCAGTGATACAC‑TACAACTTTAAAGCATTTCCA‑GCAATTATTGAGTTTGTCCTTGCCGCCGATTCCATTCTTTGTATTGTCTGTATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTACCAGCGCCACAACATCCCATATCTCTTTTAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTttatta                                                                                                                                      <  2:61482/246‑1 (MQ=255)                                                                                                                 gAAATATATATGTTAATTTTATAATAATGGTTTATTA‑AGT‑TTGTT‑CAGAAACGATCC‑GGGATACAC‑TGCAACATTAAAGCATGACCA‑GCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAAc                                                                                                                                  >  1:82435/1‑251 (MQ=255)                                                                                                                  aaaaaataatGTTATTTTTATAAAAATTGTTTATTA‑A‑T‑TTGTTTCAGAAACGAGCC‑GTGAT‑CACCTTAAACTTTAAAGCATGCCCA‑GCCATTTTTGAGTGTGGCTTGGCCGGCGATCCCATCCTTTGTATTGTCTGGATTACTGTTGATTTTTTTGCCGTTTTCTCCTTTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGGTTATTAAAACa                                                                                                                                 <  2:346828/243‑1 (MQ=255)                                                                                                                        atatTTTAATTTTATAATAATGGTTTATTA‑AGT‑TTGTT‑CAGAAACGATCC‑GGGATACAC‑TGCAACATTAAAGCATGACCA‑GCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCa                                                                                                                           <  1:218659/251‑1 (MQ=255)                                                                                                                                   tcataataATGGTTTATTA‑AGT‑TTGTT‑CAGAAACGATCC‑GGGATACAC‑TGCAACATTAAAGCATGACCA‑GCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAA‑CAGTACCAAACTCATCTGa                                                                                                                >  1:80716/3‑250 (MQ=255)                                                                                                                                         taatGGTTTATTA‑AGT‑TTGTT‑CAGAAACGATCC‑GGGATACAC‑TGCAACATTAAAGCATGACCA‑GCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGc                                                                                                          >  1:306071/1‑251 (MQ=255)                                                                                                                                         taatGGTTTATTA‑AGT‑TTGTT‑CAGAAACGATCC‑GGGATACAC‑TGCAACATTAAAGCATGACCA‑GCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGACACCACATCACATATCTCTTTCAGCTACACCGCCCGCCAGCGGATGCAAAACCGTGCGCGGTTATTAAAACAGTACCAAACTCATATGaaaacgc                                                                                                          >  2:261821/1‑248 (MQ=255)                                                                                                                                            tGGTTTATTA‑AGT‑TTGTT‑CAGAAACGATCC‑GGGAGACAC‑TGCAACATTAAAGCATGACCA‑GCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTATGGATTACTGATGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACTTAACATATCTCTTTCAGCTACGCCGACCGCCAGCGGATGCAAAACCGTGCGAGTTTATTAAAACCGTACCCAACTAATCTCAACAAGCAgg                                                                                                       >  2:287076/1‑251 (MQ=255)                                                                                                                                             ggTTTATTA‑AGT‑TTGTT‑CAGAAACGATCC‑GGGATACAC‑TGCAACATTAAAGCATGACCA‑GCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGc                                                                                                      >  1:121546/1‑251 (MQ=255)                                                                                                                                                       aGT‑TTGTT‑CAGAAACGATCC‑GGGATACAC‑TGCAACATTAAAGCATGACCA‑GCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTatat                                                                                             >  1:183549/1‑251 (MQ=255)                                                                                                                                                              tt‑CAGAAACGATCC‑GGGATACAC‑TGCAACATTAAAGCATGACCA‑GCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTgg                                                                                        >  1:312534/1‑250 (MQ=255)                                                                                                                                                                                           caaaataaatacataacctgCCATTATTGGGTAGTTCCTTGCCTCCGATTCCCTCCTTTGTATTGTCTGGATTACGTTTGATTTGTTTGCCTTTTTCTTCATTTGCCAGCGCCACCACAGCACAGATCCCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGgcg                                                              <  2:183549/232‑1 (MQ=255)                                                                                                                                                                                              aTTAAAGCATGACCA‑GCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTAc                                                          <  1:41249/251‑1 (MQ=255)                                                                                                                                                                                                             cgCCATTAATGAGTATGTCCTTGCCTTCGATTCCATCCTTTTTATTGTCTGGATTTCTGTTGATTTGTTTGCCTTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTCCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCgg                                            <  1:275784/250‑1 (MQ=255)                                                                                                                                                                                                                               ccTTGCCGTCGATTCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCAt                          <  1:12259/251‑1 (MQ=255)                                                                                                                                                                                                                                           ttCCATCCTTTGTATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGttt              >  1:186560/1‑251 (MQ=255)                                                                                                                                                                                                                                                       tATTGTCTGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGAAATGCCGAAAGCAAAAGGGAGCTACGCCAGCACAACCGGGAGCAAATAAAACAGCCTCCAGAATGGTTTCCTGCACCGCTc  >  2:108518/1‑251 (MQ=255)                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    CATTGGGGTATCTCCTGTGCTGTATATTTATTCAGCTTGAATTGTAGCTCAAATGAGCAACAGCGACAATCCATGAGTAATTTAATAAACGGATGTTTTTACATAATTAACTGAAATATATATGTTAATTTTATAATAATGGTTTATTA‑AGT‑TTGTT‑CAGAAACGATCC‑GGGATACAC‑TGCAACATTAAAGCATGACCA‑GCCATTATTGAGTATGTCCTTGCCGTCGATTCCATCCTTTGTATTGTCGGGATTACTGTTGATTTGTTTGCCGTTTTCTTCATTTGCCAGCGCCACCACATCACATATCTCTTTCAGCTACGCCGCCCGCCAGCGGATGCAAAACCGTGCGCGTTTATTAAAACAGTACCAAACTCATCTGAAAAAGCAGGCCAGCTATATTGTGGAAGGCAATGCCGAAAGCAAAAGGGCGCTACGCCAGCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCTCCGCTC  >  NC_000913/246500‑246990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑