Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,293,781 2 bp→AT coding (239‑240/597 nt) nrfG → putative formate‑dependent nitrite reductase complex subunit NrfG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,293,7810GA94.3% 89.8 / ‑2.6 35G80D (GGC→GAT) nrfGputative formate‑dependent nitrite reductase complex subunit NrfG
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (18/15);  total (19/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01
*NC_0009134,293,7820CT94.3% 95.8 / ‑1.2 35G80D (GGC→GAT) nrfGputative formate‑dependent nitrite reductase complex subunit NrfG
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (18/15);  total (19/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-01

AGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATG‑GCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGGCGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACC‑GCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACG  >  NC_000913/4293539‑4294023
                                                                                                                                                                                                                                                   ||                                                                                                                                                                                                                                                  
aGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACCCGCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATG‑GCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACt                                                                                                                                                                                                                                             <  2:95505/251‑1 (MQ=255)
      gAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATG‑GCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCtgt                                                                                                                                                                                                                                       >  1:19634/1‑251 (MQ=255)
       aaaCAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATG‑GCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCtgtg                                                                                                                                                                                                                                      <  1:232295/251‑1 (MQ=255)
            gCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATG‑GCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGGCGAGTACTATCTGTGGCaa                                                                                                                                                                                                                                  >  1:229311/1‑250 (MQ=255)
                      ccGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATG‑GCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCGGTGGCAAAACGATTACAg                                                                                                                                                                                                                       <  1:231007/251‑1 (MQ=255)
                                         cacTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATG‑GCAGGCGGTACGTGCGAAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTAc                                                                                                                                                                                                    >  1:263137/1‑251 (MQ=255)
                                             cACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATG‑GCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTc                                                                                                                                                                                                <  1:165167/251‑1 (MQ=255)
                                               cGATATTGATGGGATTTCTCTGGGTCGACAGTTATCTGTTAAGCCCAAAATG‑GCAGGCGGTACGTGCGGAGTAGCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAAGCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAg                                                                                                                                                                                              <  2:233791/251‑1 (MQ=255)
                                                   attgggggTATTTCCCTGTGTCGGCAGTTATCTGTTCAGGCCAAA‑TGTGCATGCGGTACGTGCGGAGTATAACCGTCAGCGCGATCCGCTACATCAGTTTGACAGCCAGCAAACCCCGGAAGCGGAGCTTCATGCAGTGGAGGATAAAATCCGCACTAATCCACAAAACAGCGAACAGTGGGCGTTTCTGGATGAGTACTAGCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAggtg                                                                                                                                                                                           <  2:159907/244‑3 (MQ=255)
                                                      gATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATG‑GCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGc                                                                                                                                                                                       >  1:121835/1‑251 (MQ=255)
                                                          gTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATG‑GCAGGCGGGACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTCCTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCCGGCGTTGCAACt                                                                                                                                                                                   >  1:307970/1‑251 (MQ=255)
                                                                 tctGTGTCGGCAGTTATCTGGTAAGCCCAAAATG‑GCAGGCGGTACGGGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACACAACAGCGAACAGAGGGCGTTACTGGATGAGTACTATCTTTGGCAAAACGATTAAAGAATTTCATTGCTGGCGTACCTTCAGGCGTTGCGACTACgaggg                                                                                                                                                                            >  2:135631/1‑247 (MQ=255)
                                                                    gtgtCGGCAGTTATCTGTTAAGCCCAAAATG‑GCAGGCGGGACGTGCGGAGTATCAGCGTCAGCGCGATCAGCTAAATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTAATGGATGAGTACTACCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGAAACTCCgtggaga                                                                                                                                                                          >  2:243836/1‑247 (MQ=255)
                                                                                   tGTTAAGCCCAAAATG‑GCAGGCGGTACGAGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCCGCCAGCAAACCCCGGATGCGCAGCTTCAGGCATTGCAGGATAAACTCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAAAGATTACAGCCATTCGTTGCTGGCGTCACGTCAGGCGTTGCAACTACTGGGTGAGAACgatagactgaat                                                                                                                                                          >  2:37745/1‑240 (MQ=255)
                                                                                       aaGCCCAAAATG‑GCAGACGGTACGTGCGGAGTATCCGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAACCGATTACAGCAATTCGATGCTGGCGTACCGTCAGGCGTTGCAACTACGGGGTGAGAACGCttgaatgtatgggg                                                                                                                                                      >  2:199184/1‑238 (MQ=255)
                                                                                        aGCCCACAATG‑GCAGGCGGTACGTGCGTAGTATCAGCGTCAGCGCGATCCGCTACATCAGGTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTTCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATgcggc                                                                                                                                                     <  1:27615/251‑1 (MQ=255)
                                                                                                                 gctgAGTATCAGCGTCAGCACGATCCGCTACAACAGTTTGCCAGCCAGCAAACCCCGGAAGCGCATCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGTGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTAGTACCa                                                                                                                             <  2:19634/248‑1 (MQ=255)
                                                                                                                 gCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGCACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCa                                                                                                                             <  1:188647/251‑1 (MQ=255)
                                                                                                                  cggctgatcagctcccacgcgCTCCGCGACATCAGTGTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAAGCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGGCGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGGTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGTTGCTTTAATACCat                                                                                                                            <  2:229311/234‑2 (MQ=255)
                                                                                                                      gTATCCGCGTAAGCGAGATCCGCCACATCAGTTTGCAAGCCAGCAACCCCCGGCAGCGCAGCTTCAGGCAATGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGCACAGTGGGCGGTACTGGATGAATACTATCTGTAGCAAAACGATTACAGCAATTCGTTGGTGGAGTACCGTCAGGCGTTGCAATTCCGAGGGGCGAACGCTGAACTGTATCTGGCGTTGGCGACGGTGCTTTTTTAACAgagc                                                                                                                         >  2:313549/1‑247 (MQ=255)
                                                                                                                               tcagcgCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGCTTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGGGCTTTATTACCAGGCCAGCCAGCat                                                                                                                >  2:340006/1‑250 (MQ=255)
                                                                                                                                   gtggacccgctgaaTCAGGTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGAAGGATAAAAGCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTATTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGAc                                                                                                           <  2:70218/238‑1 (MQ=255)
                                                                                                                                      gATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCGGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGGCGGTGCTTTATTACCAGGGCCGCCAGCatattgccgc                                                                                                        >  2:49938/1‑245 (MQ=255)
                                                                                                                                                   aGTTTGCCAGCCAGCACACCACGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGAGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTCCAGCAATTCGTTCCTGGCGTACCGTCAGGCGTTGCAACTACGTGGCGTGGACGCTGAAATGCATGAGGCGCTGGCGCCGTTGCTTTATTAACAGGCCAACCACCACAT‑ACCAGCCCATACTCgcgc                                                                                           >  2:279751/1‑250 (MQ=255)
                                                                                                                                                       tGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACC‑GCCCAGACTCGCGCAATg                                                                                       <  1:37745/250‑1 (MQ=255)
                                                                                                                                                                 cAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGCACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGAATATGACC‑GCCCAGACTCGCGCAATGAACGACAAAGc                                                                            >  2:99135/1‑251 (MQ=255)
                                                                                                                                                                  aaaCCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACC‑GCCCAGACTCGCGCAATGATCGACAAAGcc                                                                           >  1:241750/1‑251 (MQ=255)
                                                                                                                                                                         ggAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAACCGATTACAGCACTTCGTTGCTGGAGTACCGTAAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTTCTTTATTACCAGGCCCGCCAGCATATGACC‑GCCCAGACTCGCGCAATGATCGACACAGCCCTCgcgc                                                                    >  1:147783/1‑251 (MQ=255)
                                                                                                                                                                           aaGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACC‑GCCCAGACTCGCGCAATGATCGACAAAGCCCTCccgccg                                                                  >  2:200213/1‑249 (MQ=255)
                                                                                                                                                                                 aGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACC‑GCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGt                                                            >  1:314616/1‑251 (MQ=255)
                                                                                                                                                                                                        aTCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACC‑GCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGAtgct                                     >  1:278235/1‑251 (MQ=255)
                                                                                                                                                                                                            gcgcTAATCCCCAAAACAGCGAAAAGTGGGCTTTACTGGATGAGTTCTACCTGTGGCAAAACGTTGTCAGCAAGTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAGCGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACC‑GCCTAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAACTCACCGCCCTTATGcttctg                                 <  2:87688/251‑1 (MQ=255)
                                                                                                                                                                                                                       caAAACAGCGAACAGTGGGCGTTACTGGATGAGTACTATCGGTGGCAAAACGATTACAGCTATTCGTTGCGGGCGTACCTTCAGGCGTTGCAACTACGTGGTGTGAACGCTGATCTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACC‑GCCCAGACTCGCGCAAGGATCGACAAAGCCCTCGCGCTGGGCAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGc                      <  2:272664/251‑1 (MQ=255)
                                                                                                                                                                                                                                   cAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACC‑GCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGc          <  1:46152/251‑1 (MQ=255)
                                                                                                                                                                                                                                           gTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTCCCAGGCCAGCCAGCATATGACC‑GCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTAcg  <  2:121835/251‑1 (MQ=255)
                                                                                                                                                                                                                                                   ||                                                                                                                                                                                                                                                  
AGCGATGAAACAGCCCAAAATACCGGTGAAAATGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATG‑GCAGGCGGTACGTGCGGAGTATCAGCGTCAGCGCGATCCGCTACATCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGCGAACAGTGGGCGTTACTGGGCGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACC‑GCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACG  >  NC_000913/4293539‑4294023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑