Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,474,539 T→C N5S (AAC→AGC)  rpsL ← 30S ribosomal subunit protein S12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,474,5390TC90.6% 86.4 / 0.9 32N5S (AAC→AGC) rpsL30S ribosomal subunit protein S12
Reads supporting (aligned to +/- strand):  ref base T (3/0);  new base C (13/16);  total (16/16)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01

GTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAATATACAACGATCCCGCCATCACCAGGCCATCTGGCTGGGGTGCTTAACCGTAAGTCTGACGAAATCAGTATAGTCAATGAGAATGATGTCGTTCGAAATTTGACC  >  NC_000913/3474337‑3474764
                                                                                                                                                                                                          |                                                                                                                                                                                                                                 
gtgACCTTCACCACCGATGTAGGAAGTAACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGTGAGTACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTac                                                                                                                                                                                    >  2:168961/1‑249 (MQ=255)
             ccGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAAc                                                                                                                                                                      >  2:267970/1‑251 (MQ=255)
               gATGTAGGAAGTCACTTCGAAACCTGTAGTCAGACGAACCCGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATaaaa                                                                                                                                                                       <  2:238606/248‑1 (MQ=255)
               gATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATaaaa                                                                                                                                                                       >  1:238606/1‑248 (MQ=255)
                  gTAGGAAGTCAATTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTACTGGTTTTAGCTTTTGCTTCGTAAACACGAAATAAAACGTCCt                                                                                                                                                                 >  1:331124/1‑251 (MQ=255)
                       aaGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTcacac                                                                                                                                                            >  2:165626/1‑251 (MQ=255)
                               tCGAAGCCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGa                                                                                                                                                     <  1:268863/250‑1 (MQ=255)
                                   aaCCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACg                                                                                                                                                <  1:20645/251‑1 (MQ=255)
                                     ccgttaggaagacgaatattgCATACTGTACGCAGCGCGGAGTTCGTGTTTGTAGGAGTGTTAGTAGATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCAGGTTGCTTTTCGCAACTTTGCGAGCACGTGGGTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGTTCTTGGTTTTAGCTTTTGCCTCGTAAACACGTAATAATACGTCTTCACACAATAGGAGGACGcc                                                                                                                                              <  2:155698/231‑1 (MQ=255)
                                        ttAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCAAACAATATGAGGACGCCGa                                                                                                                                            >  1:31519/1‑250 (MQ=255)
                                           gTCAGACGAACATGGCATACTTTACGCAGCGCGTAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTGTAGCTTTTGCTTCTTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAAttt                                                                                                                                        <  1:224463/251‑1 (MQ=255)
                                                  gAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAg                                                                                                                                     <  1:153318/247‑1 (MQ=255)
                                                  gAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGCAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATCGATCATGGTTTTCGCTTTTGCTTCGTAAACACGTAATAACACGTCCTCACACACTCTGTGGACGCCGAATTTTAg                                                                                                                                     >  2:153318/1‑247 (MQ=255)
                                                       cGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGcc                                                                                                                            <  1:91083/251‑1 (MQ=255)
                                                           aTACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGaaa                                                                                                                        <  2:31519/251‑1 (MQ=255)
                                                                        cgcgGAGTACGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGaaa                                                                                                           <  1:264195/251‑1 (MQ=255)
                                                                                        gtAGGAGTGGTAGTATATACCCGAGTACATTCGCCGCGTATGTGCGTGCATGCTTCCGGCGCAGGCCCGGTGCTTTTCGCAACTTTGAGAGCTCGTGGTTTGCGTTCCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCGCACACAATATGATGACGCCGAATTTTAGGGAGATGCCGAATAGGTGGCATGAAATATACAACGATCCCg                                                                                            <  2:56402/249‑1 (MQ=255)
                                                                                           ggAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGCAACGGTGTCAAGAAATATACAACGATCCCGCCAt                                                                                        >  2:93567/1‑251 (MQ=255)
                                                                                             aGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAATATCCAACGAt                                                                                                >  2:178308/1‑241 (MQ=255)
                                                                                             aGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAATATACAACGAt                                                                                                <  1:178308/241‑1 (MQ=255)
                                                                                                ggTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAATATACAACGATCCCGCCATCACCa                                                                                   <  1:83777/251‑1 (MQ=255)
                                                                                                 gtagtaTATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCCCACAATATGAGGACGACGAATTTTAGGGCGCTGCCGAAAAGGTGTCAAAAAATCTACAACGATCCCGCCATCAcc                                                                                    >  2:298514/1‑249 (MQ=255)
                                                                                                                  aCATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAATATACAACGATCCCGCCATCACCAGGCCATCTGGCTGGGGTg                                                                 >  1:31527/1‑251 (MQ=255)
                                                                                                                     aactcctagTTTTTGCGTGACTGCTTCAAGCGCAGTCTCGTTGCTGTTCGTAACTTGGTGAGTACGGTGTTAGGGTACCAGGTGGCTAACTGTATCCATTAAATAGGTCTTGGTTTTAGCTTTGGCTTCGTAACCACGTAATAAAACGACTACACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGGCAAGAAATATCCAACGCTCCCGCCATCTCCAGGCCATCTGGCTGGGGTGCtt                                                              <  2:260082/243‑1 (MQ=255)
                                                                                                                               ttgtGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAATATACAACGATCCCGCCATCACCAGGCCATCTGGCTGGGGTGCTTAACCGTAAg                                                     <  1:95271/247‑1 (MQ=255)
                                                                                                                                       gCATGCTTCCAGCGCAGGCACGATGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGACGATGCCGAAAAGTGGTCAAGAAATATACAACGATCCCGCCATCACCAGGCCATCTGGCTGGGGGGCTTAACCGTAAGTCTGACGa                                             >  2:283123/1‑250 (MQ=255)
                                                                                                                                         aTGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGAt                                                                                                                               <  1:177545/166‑1 (MQ=255)
                                                                                                                                         aTGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGAt                                                                                                                               >  2:177545/1‑166 (MQ=255)
                                                                                                                                                       ggCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATGTGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAATATACAACGATCCCGCCATCACCAGGCCATCTGGCTGGGGTGCTTAACCGTACGTCTGACGAAATCAGAATAGTCAATg                            >  2:58333/1‑251 (MQ=255)
                                                                                                                                                                         aCTTTGCGAGCACGAGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAGTAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGAAATAAAACGTCCTCACACAATATGAGGACGCCGCATTTTAGGGCGATGCCGCAAAGGTGTCAAGGAATATACAGCGATCCCGACATCACCAGGCCATCTGGCTGGGGTGCTTAAACGTATGTCTGACGAAATCAGTATAGGCAGTGAGAATGATGTCGGTCGa           >  2:150038/1‑250 (MQ=255)
                                                                                                                                                                          cTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAACACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAATATACAACGATCCCGCCATCACCAGGCCATCTGGCTGGGGTGCTTAACCGTAAGTCTGACGAAATCAGTATAGTCAATGAGAATGATGTCGTTCGaa          >  1:303002/1‑250 (MQ=255)
                                                                                                                                                                                 aGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAATATACAACGATCCCGCCATCACCAGGCCATCTGGCTGGGGTGCTTAACCGTAAGTCTGACGAAATCAGTATAGTCAATGAGAATGATGTCGTTCGAAATTTGAcc  <  1:267970/251‑1 (MQ=255)
                                                                                                                                                                                                          |                                                                                                                                                                                                                                 
GTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAATATACAACGATCCCGCCATCACCAGGCCATCTGGCTGGGGTGCTTAACCGTAAGTCTGACGAAATCAGTATAGTCAATGAGAATGATGTCGTTCGAAATTTGACC  >  NC_000913/3474337‑3474764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: