| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,410,419 | G→A | L312L (CTG→TTG) | yfbS ← | putative transporter YfbS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,410,419 | 0 | G | A | 95.0% | 43.5 / NA | 21 | L312L (CTG→TTG) | yfbS | putative transporter YfbS |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (10/9); minor base C (1/0); total (11/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCG‑CAGTACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCA > NC_000913/2410207‑2410655 | aaCCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCG‑CACTACCATCGGCTCCAGCAATTGCTCACTACAAAATTGcc > 1:184160/1‑251 (MQ=255)aaCCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGACCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCG‑CAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGcc > 1:146917/1‑251 (MQ=255) aGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCG‑CAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGgag < 1:139409/251‑1 (MQ=255) ggATGATATCGCCCCGCCGCCGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCG‑CAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGgaga < 2:104381/251‑1 (MQ=255) gatAACGCCCGGCAGCATAGCCTCCACCGCCAGCGACCCTTCCAGCGCCTCGCAATTGCGCTTGAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGAGCCGAGTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCGCATCAAGGGCCTTGTCAGAAAAATACTCGCCGCG‑CAATACCAGCGGCTCCAGCAAGTGCTCGCTTCAAAATTGCCGGAGATCGa < 2:211801/251‑1 (MQ=255) gcagAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCG‑CAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGAc < 1:67459/251‑1 (MQ=255) cagtcgcacgCGAACCTTCCAGCCTCACGCCATTGCGCTTGAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATCGACCAATCAGTTCTGACGCAGGAACTAATGAAATCTCTGCCATGCCCACATTAAGGGCCTGGTCAGAAAAATACTCGCCGCT‑CAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCAGTAGATCGACATTATCCGCTGACATATCAATa < 2:57817/242‑1 (MQ=255) cGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGGCAGAAAAATACTCGCCGCG‑CAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACAAATCAATAAGc > 2:185753/1‑250 (MQ=255) cAGCGAACCTTCAAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTAAGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCG‑CAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACAACAGACGCAGACATATAAATAAGaaaa > 2:1382/1‑250 (MQ=255) ccTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCG‑CAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCAcg < 1:185753/251‑1 (MQ=255) gccaTTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTAGCGCACCGATTTACCAATCAGTTCCGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCG‑CAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGAGAAATTc > 1:238345/1‑249 (MQ=255) cGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCG‑CAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGcgacg > 1:295254/1‑250 (MQ=255) gggTACGAAATCAAATTTCGCGCCCCTATTTACCAACCAGTTCTGACTCAGGAATTAATGAAACCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCG‑CAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCTAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAAcgc < 2:261812/251‑1 (MQ=255) cGATTTACCAATCAGTTCTGAATCAGGAATTAATGAAATCTATGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCG‑CAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGt > 1:277629/1‑251 (MQ=255) tttACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCG‑CAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGtt > 1:221061/1‑249 (MQ=255) aCCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCG‑CAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATACCGCGACGAAAACGCCGCCAGCGTTCAACACCTATGACGTTAgcgc > 2:102872/1‑251 (MQ=255) agtaataaAATCTCCGTCCTGCCCCCATCAAGGGTCTGGTCATAAAAATGCTCGCCTCGACA‑TACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGAGCGACATCAGCCGCACACATATCAATAAGCAAAACGTCACTCGCGCGAAATTCAGAAAACCCATTAACGTCCACGTTAACGCGACGAAAACGCCGCCAGCGTTCAACGCCGATGACGTTAGCGCCATAACGCTCACGTAATTTgag < 2:82044/249‑1 (MQ=255) aaGGGCCTGGTCAGAAAAAGACTCGCCGCG‑CAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCg < 2:132083/251‑1 (MQ=255) tGGTCAGAAAAATACTCGCCGCG‑CAATACCATCGGCTCCAGCAATTGCTAACTACAAAATTGCCGGAGATCGACATCAGCCGCAGCCATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCAAAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCgcatcggg > 2:233773/1‑244 (MQ=255) aaTACTCGCCGCG‑CAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGcc > 1:304732/1‑251 (MQ=255) aTACTCGCCGCG‑CAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCa < 1:94870/251‑1 (MQ=255) | AACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCG‑CAGTACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCA > NC_000913/2410207‑2410655 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |