Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,108,758	T→C	intergenic (+244/‑76)	pfkA → / → sbp	6‑phosphofructokinase 1/sulfate/thiosulfate ABC transporter periplasmic binding protein Sbp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,108,758	0	T	C	100.0%	70.5 / NA	23	intergenic (+244/‑76)	pfkA/sbp	6‑phosphofructokinase 1/sulfate/thiosulfate ABC transporter periplasmic binding protein Sbp
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/14);  total (9/14)

TACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGCGCTTCGCTTATCAGGCCTACATGAATTCTGCAATTTATTGAATTTGCAAACTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATGGACAAAGCGCACTTTGTCAGCAATATGAGGCGGATTTCTTCCGC‑CTTTTTAATCCCTCAACATATACCCGCAAGTTATAGCCAATCTTTTTTTATTCTTTAATGTTTGGTTAACCTTCTGGCACGCTTTGCTCATCACAACACAA‑CATAAGAGAGTCGGGCGATGAACAAGTGGGGCGTAGGGTTAACATTTTTGCTGGCGGCAACCAGCGTTATGGCAAAGGATATTCAGCTTCTTAACGTTTCATATGATCCAACGCGCGAATTGTACGAACAGTACAACAAGGCATTCAGCGCCCACTGGAAACAGCAAACTGGTGATAACG  >  NC_000913/4108541‑4108996                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                tACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGCGCTTCGCTTATCAGGCCTACATGAATTCTGCAATTTATTGAATTTGCAAACTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATGGACAAAGCGCACTTTGTCAGCAATATGAGGCGGATTTCTTCCGC‑CTTTTTAATCCCTCAACATATACCCGCAAGTTATAGCCAATCCTTTTTTATTCTTTAATGTTTGGTTAACCTTCt                                                                                                                                                                                                                 <  1:49411/250‑1 (MQ=255)       gAAACCGATGACAGAAGCAAAAATGCCTGATGCGCTTCGCTTATCAGACCTACATGAATTCTGCAATTTATTGAATTTGCAAACTTTTGTAGGCCGGATAAGGCGTTCGCTCCGCATCCGGCATGGACAAAGCGCACGTTGTCAGCAATATGAGGCGGATTTCTTCCGC‑CTTTTTAATCCCTCAACATATAACCGCAAGATATAGCCAATCCTTTTTTTTTCTTTAATGTTTGGTTAACCTTCTGGCActc                                                                                                                                                                                                          >  2:211146/1‑249 (MQ=255)                    aaatcAAAAATGCCTGATGCGCTTCGCTTATCAGGCCTACATGAATTCTGCAATTTATTGAATTTGCAAACTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATGGACAAAGCGCACTTTGTCAGCAATATGAGGCGGATTTCTTCCTC‑CTTTTTAATCCCTCAACATATACCCGCAAGTTATAGCCAATCCTTTTTTATTCTTTAATGTTTGGTTAACCTTCTGGCACGCTTTGCTCATcaca                                                                                                                                                                                             <  1:204926/247‑1 (MQ=255)                                      gcgcTTCGCTTATCAGGCCTACATTAATTCTGCAATTTATTGAATTTGCAAACTTTTGTAGGCCGTATAAGGCGTTCGCGCCGCATCCGGCATGGACAAAGCGCACTTTGTCAGCAATATGAGGCGGATTTCTTCCGC‑CTTTTTAATCCCTCAACATATACCCGCAAGTTATAGCCAATCCTTTTTTATTCTTTAATGTTTGGTTAACCTGCTGGCACGCTTTGCTCATCACAACACAA‑CATAAGAGAGt                                                                                                                                                                           <  1:157306/250‑1 (MQ=255)                                                         tACATGAATTCTGCAATTTATTGAATTTGCAAACTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATGGACAAAGCGCACTTTGTCAGCAATATGAGGCGGATTTCTTCCGC‑CTTTTTAATCCCTCAACCTATACCCGCAAGTTATAGCCAATCCTTTTTTATTCTTTAATGTTTGGTTAACCTTCTGGCACGCTTTGCTCATCACAACACAA‑CATAAGAGAGTCGGGCGATGACCaa                                                                                                                                                             >  2:142174/1‑245 (MQ=255)                                                         tACATGAATTCTGCAATTTATTGAATTTGCAAACTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATGGACAAAGCGCACTTTGTCAGCAATATGAGGCGGATTTCTTCCGC‑CTTTTTAATCCCTCAACATATACCCGCAAGTTATAGCCAATCCTTTTTTATTCTTTAATGTTTGGTTAACCTTCTGGCACGCTTTGCTCATCACAACACAA‑CATAAGAGAGTCGGGCGATGAACaa                                                                                                                                                             <  1:142174/245‑1 (MQ=255)                                                            aTGAATTCTGCAATTTATTGAATTTGCAAACTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATGGACAAAGCGCACTTTGTCAGCAATATGAGGAGGATTTCTTCCGC‑CTTTTTAATCCCTCAACATATACCCGCAAGTTATAGCCAATCCTTTTTTATTCTTTAATGTTTGGTTAACCTTCTGGCACGCTTTGCTCATCACAACACCA‑CATAAGAGAGTCGGGCGATGAACAAGGGGGGCGt                                                                                                                                                    >  1:11679/1‑251 (MQ=255)                                                               aaTTCTGCAATTTATTGAATTTGCAAACTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATGGACAAAGCGCACTTTGTCAGCAATATGAGGCGGATTTCTTCCGC‑CTTTTTAATCCCTCAACATATACCCGCAAGTTATAGCCAATCCTTTTTTATTCTTTAATGTTTGGTTAACCTTCTGGCACGCTTTGCTCATCACAACACAA‑CATAAGAGAGTCGGGCGATGAACAAGTGGGGCGTAgg                                                                                                                                                 <  1:344355/251‑1 (MQ=255)                                                                atacagCAATATAATGAATGTGCAAACTTTTGTAGGCCGGATAAGGGTTTCGCGGCGCCACCGGAAGGGACAAAGCGTACTTTGTCAGCAATAAGAGTCGGATTTCTTCCGC‑CTTTTTAATCCCTCATCATATACCCGCAAGTTATAGCCAATCCGTTTTTATTCTTGAATGTTTGGTTAACATTCCGGCACGGGTTGCTCGGTACTACATAA‑CATAAGAGAGTCGGGCGATGAACAAGTGGGTCGTAggg                                                                                                                                                <  2:176025/246‑1 (MQ=255)                                                                 ttCTGCAATGTATTGAATTTGCAAACTTTTGTAGGCCGGATAAGGCGTTCGCGACGCATCCGGAATGGACAAAGCGCACTTTGTCAGCAATATGAGGCGGATTTCTTCCGC‑CTTTTTAATCCCTCAACATATAACAGCCAGGTATATCCAAACCATTTTTATTCTTTAATTTTTGGTTCACCTTCGTGCACGCTTTGCTCATCACAA‑ACAAACATAAATGCGTAGGGCGATATACACGTGGGGCGTAAGGt                                                                                                                                               >  2:113320/1‑251 (MQ=255)                                                                      cAATTTATTGAATTTGCAAACTTTTGTAGGGCGGATAAGGCGTTCGCGCCGCATCAGGCATGGACAAAGCGCAATTTGTCAGAAATATGAGGCGGATTTCTTCCGC‑CTTTTTAATCCCTCAACCTATACCCGCAAGTTATAGCCACTCCTTTTTTATTCTTTACTGTTTGGTTCACCTTCTGGCACGCTTTGCTCATCACCAAACAA‑CATAAGCGCGTCGGGCGATGCACAAGTGTGGCGTAGGGTTAAc                                                                                                                                           >  1:43899/1‑250 (MQ=255)                                                                                                tAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATGGACAAAGCGCACTTTGTCAGCAATATGAGGCGGATTTCTTCCGC‑CTTTTTAATCCCTCAACATATACCCGCAAGTTATAGCCAATCCTTTTTTATTCTTTAATGTTTGGTTAACCTTCTGGCACGCTTTGCTCATCACAACACAA‑CATAAGAGAGTCGGGCGATGAACAAGTGGGGCGTAGGGTTAACATTTTTGCTGGCGGCAACCAGCGTTAt                                                                                                                >  1:70487/1‑251 (MQ=255)                                                                                                  ggCCGGATAAGGCGTGCGCGCCGCATCCGGCATGGACAAAGTGCACTTTGGCAGCAATATGAGGCGGATTTCTTCCGC‑CTTTTTAATCCCTCAACATATACCCGCAAGTTATAGCCAATCCTTTTTTATTCTTTAAGGTTTGGTTAACCTTCTGGCACGCTGTGCTCATCACAACACAA‑CATAAGAGAGTCGGGCGATGAACAAGTGGGGCGTAGGGTTAACATTTTTGCTGGCGGCAACCAGCGTTATgg                                                                                                              <  2:221860/251‑1 (MQ=255)                                                                                                                        gaaTCCGTCATGGACAAAGCTCCCTTTGTCAGCAATATGGTGCGGATTTCTTCCGC‑TTTTTTAATCCCCCAACATATCCCCGCAAGTTTTATCCAATCCTTTTTTATTCTTTAATGTTTGGTTAACCTTCTGGCACGCTTGGCTCATCACAACACAA‑CATAAGAGAGTCGGGCGATGAACAAGTGGGGCGTAGGGTTAACATTTTTGCTGGCGGCAACCAGCGTTATGGCAAAGGATATTCAGCTTCTTaa                                                                                        <  2:83079/249‑1 (MQ=255)                                                                                                                                         aGCGCACTTTGTCAGCAATATGAGGCGGATTTCTTCCGC‑CTTTTTAATCCCTCAACATATACCCGCAAGTTATAGCCAATCCTTTTTTATTCTTTAATGTTTGGTTAACCTTCTGGCACGCTTTGCTCATCACAACACAA‑CATAAGAGAGTCGGGCGATGAACAAGTGGGGCGTAGGGTTAACATTTTTGCTGGCGGCAACCAGCGTTATGGCAAAGGATATTCAGCTTCTTAACGTTTCATATGATCCaa                                                                       <  1:11987/251‑1 (MQ=255)                                                                                                                                                 ttGTCAGCAATATGAGGCGGATTTCTTCCGC‑CTTTTTAATCCCTCAACATATACCCGCAAGTTATAGCCAATCCTTTTTTATTCTTTAATGTTTGGTTAACCTTCTGGCACGCTTTGCTCATCACAACACAA‑CATAAGAGAGTCGGGCGATGAACAAGTGGGGCGTAGGGTTAACATTTTTGCTGGCGGCAACCAGCGTTATGGCAAAGGATATTCAGCTTCTTAACGTTTCATATGATCCAACGCGCGa                                                                >  1:339407/1‑250 (MQ=255)                                                                                                                                                      aGCAATATGAGGCGGATTTCTTCCGC‑CTTTTTAATCCCTCAACATATACCCGCAAGTTATAGCCAATCCTTTTTTATTCTTTAATGTTTGGTTAACCTTCTGGCACGCTTTGCTCATCACAACACAA‑CATAAGAGAGTCGGGCGATGAACAAGTGGGGCGTAGGGTTAACATTTTTGCTGGCGGCAACCAGCGTTATGGCAAAGGATATTCAGCTTCTTAACGTTTCATATGATCCAACGCGCGAATTGTa                                                          <  2:70487/251‑1 (MQ=255)                                                                                                                                                                   ggATTTCTTCCGCTCTTTTTA‑TCCCTACACCTATTCCCTCTAGTTATAGCCAATCCTTTTTTATTCTTTAATGTTTGGTTAACCTTCTGGCTCGCTTTGCTCATCACAACGCAA‑CTAAAGAGAGTCGGGCGACTAACAAGTTGGGCGTAGGATGAACATTTTTTCTGTCGGCAACCAGCGTTATGGCAAAGGATATTCAGCTTCTTAACGTTTCATATGATCCAACGCTAGAATTGTACGAACAGTactac                                             <  2:83408/251‑4 (MQ=255)                                                                                                                                                                    caTTTCTTCCGC‑CTTTTTAATCCCTCAACATATACCCGCAAGTTATAGCCAATCCTTTTTTATTCTTTACTGTTTGGTTAACCTTCTGGCACGCTTTGCTCATCACAACACAA‑CATAAGAGAGTCGGGCGATGAACACGTGGGACGTAGGGTTAACATTTTTGCTGGCGGCAACAAACGTTATGGCCAAGGATATTCAGCTTCTTAACGTTTAATCTGATCCACCCCGCGAATTGtccacaacttaacaa                                             >  2:269631/2‑236 (MQ=255)                                                                                                                                                                      tttCTTCCGC‑CTTTTTAATACCTCAACATATAACCGCAAGTTATAGCCAATCCTTTTTTATTCTTTAATGTTTGGTTAACCTTCTGGCACGCTTTGCTCATCACAACACAA‑CATAAGAGAGTCGGGCGATGAACAAGTGGGGCGTAGGGTTAACATTTTTGCTGGCGGCAACCAGCGTTATGGCAAAGGATATTCAGCTTCTTAACGTTTCATATGATCCAACGCGCGAATTGTACGAACAGTacaaca                                            >  2:177123/1‑249 (MQ=255)                                                                                                                                                                                   ttttAATCCCTCAACATATACCCGCAAGTTATAGCCAATCCTTTTTTATTCTTTAATGTTTGGTTAACCTTCTGGCACGCTTTGCTCATCACAACACAA‑CATAAGAGAGTCGGGCGATGAACAAGTGGGGCGTAGGGTTAACATTTTTGCTGGCGGCAACCAGCGTTATGGCAAAGGATATTCAGCTTCTTAACGTTTCATATGATCCAACGCGCGAATTGTACGAACAGTACAACAAGGCATTCAgcg                                <  1:211146/249‑1 (MQ=255)                                                                                                                                                                                        aTCCCTCAACTTATACCCCCAAGTTATAGCCAATCCTTTTTTATTCTTTTATGTTTGGTTAACCTTCTGGCCCGCTTTGCTCATCACAACACAA‑CATAAGAGAGTCGGGCGTTGAACAAGTGGCGCGTAGGGTTACCATTTTTGCTTGCGGCAACCAGCGTTATGGCAAAGGATATTCAGCTTCTTAACGTTTCATATGATCCAACGCGCGAATTGTACGAACAGTACAACAAGGCATTCAGCGCCCACTg                         <  1:245943/251‑1 (MQ=255)                                                                                                                                                                                                                tataGCCAAACCCTTTTTATTCTTTAATGTTTGGTTAACCTTCTGGCACGCTTTGCTCATCACAACACAA‑CATAAGAGAGTCGGGCGATGAACAAGTGGGGCGTAGGGTTAACATTTTTGCTGGCGGCAACCAGCGTTATGGCAAAGGATATTCAGCTTCTTAACGTTTCATATGATCCAACGCGCGAATTGTACGAACAGTACAACAAGGCATTCAGCGCCCACTGGAAACAGCAAACTGGTGATAACg  <  1:296333/250‑1 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                TACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGCGCTTCGCTTATCAGGCCTACATGAATTCTGCAATTTATTGAATTTGCAAACTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATGGACAAAGCGCACTTTGTCAGCAATATGAGGCGGATTTCTTCCGC‑CTTTTTAATCCCTCAACATATACCCGCAAGTTATAGCCAATCTTTTTTTATTCTTTAATGTTTGGTTAACCTTCTGGCACGCTTTGCTCATCACAACACAA‑CATAAGAGAGTCGGGCGATGAACAAGTGGGGCGTAGGGTTAACATTTTTGCTGGCGGCAACCAGCGTTATGGCAAAGGATATTCAGCTTCTTAACGTTTCATATGATCCAACGCGCGAATTGTACGAACAGTACAACAAGGCATTCAGCGCCCACTGGAAACAGCAAACTGGTGATAACG  >  NC_000913/4108541‑4108996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑