Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,729,955 2 bp→AA coding (810‑811/1323 nt) xylA ← xylose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,729,9550GA92.3% 97.6 / 0.1 40H271Y (CAC→TAC) xylAxylose isomerase
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (18/18);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01
*NC_0009133,729,9560GA92.5% 110.4 / 1.2 40N270N (AAC→AAT) xylAxylose isomerase
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (19/18);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.70e-01

ATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGG‑TTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACG  >  NC_000913/3729713‑3730195
                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                                 
agtatatTTATCAGTTCTTTGACGCCTTTTTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGGATTCTCTTCCTCACTGTTTGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCt                                                                                                                                                                                                                                             <  1:311241/247‑1 (MQ=255)
      tttATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCTTCCATGt                                                                                                                                                                                                                                      >  1:192948/1‑251 (MQ=255)
               atatggacgccGTACTTTTGCATCGAAGTTCAGACCACCGGTGGTGAACCCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCATCGTCGCGTAATTAGCTTCAATGTTCAGTTTaa                                                                                                                                                                                                                             <  1:106154/246‑1 (MQ=255)
                            ccTTTGGCATCGAAGTTCAGACCCCCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAg                                                                                                                                                                                                                <  1:7599/250‑1 (MQ=255)
                            aCTTTGGCATCGAAGTTCAGACCTCCGGTTGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCTAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTTACCTGCCTGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCa                                                                                                                                                                                                                 <  1:196607/250‑1 (MQ=255)
                                       gAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAAATGtt                                                                                                                                                                                                     >  1:345384/1‑251 (MQ=255)
                                        aaGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTT‑CCAGACCAAACTGTTTc                                                                                                                                                                                                   <  1:97941/251‑1 (MQ=255)
                                                      ggtggtGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAg                                                                                                                                                                                                                <  1:295601/225‑1 (MQ=255)
                                                      ggtgggGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGTACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGACCCGCACAGGCCTAGCGCAATGGCGGTGGCTATTTCATGGTGGAAAGAGTGACCTGCCAGCGGCGCGGAATTAGCTTCATTGTTCTGTTTAATATCTTTTTTCAGagatcggtggagcgtggtgtagggt                                                                                                                                                                                       >  2:295601/1‑226 (MQ=255)
                                                            gAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTc                                                                                                                                                                                 <  1:269312/250‑1 (MQ=255)
                                                                            agaATTTCATACAACCCCAGCGCACTCTCTTCCACACTGTGCGGGAACTGGTCGGTGTCCCAGCCCCGTTGCGCACTGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGTCCAAACTGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCata                                                                                                                                                               <  2:64424/251‑1 (MQ=255)
                                                                                    aTACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCATATTGATGtt                                                                                                                                                       <  1:117919/251‑1 (MQ=255)
                                                                                     tACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAACCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGCACAGGCCAAGCGCAATGGCGAAGGCTATTTCATGATGGAAAGAGGGACCTGCCAGCTGCACGGAATTCACTTCAATGATCAGATTAATATCTTTTTCCCAACCAAACTGTTTCAGGAAGCCATAGAACGTcg‑caccagagtaaacatatttatggtt                                                                                                                                                      >  1:333104/1‑227 (MQ=255)
                                                                                      aCATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCATATTGCTGTTTg                                                                                                                                                     >  1:329482/1‑251 (MQ=255)
                                                                                                    ttCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGTCAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCgg                                                                                                                                       >  1:215087/1‑251 (MQ=255)
                                                                                                             ccacTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGG‑TTc                                                                                                                             <  2:57803/250‑1 (MQ=255)
                                                                                                              cacTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCATATTGATGTTTGGTCGGt                                                                                                                                               <  1:56824/233‑1 (MQ=255)
                                                                                                              cacTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCGATTTCATGGTGGGAAGAGGGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTTCAGACCAAACTGTTTCCGGAAGCCATAGACCGTCG‑AGGCCTTGTAATTATATTTGTGTTTTGTCgggagatcggaagaacgtcgg                                                                                                                             >  2:56824/1‑232 (MQ=255)
                                                                                                                cTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCCCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGGCG‑CGGCATCGGAATCATATTGGTGTTTGGTCGGTTCTTGCGGTTTTGG‑TTCGAt                                                                                                                          >  1:17593/1‑251 (MQ=255)
                                                                                                                  gTTCGGGAACTGGTCGGTGGCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGG‑TTCGATaa                                                                                                                        <  1:68848/251‑1 (MQ=255)
                                                                                                                       ggAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCCCCACGGATGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTAATGATGGAAAGGGTGACCTGCCAGCGGCGCGTCATTCGCTTCAATGGTACGTTTAACATCTTTTTCCAGCCCCAACTGTTTCAGGATGCCCTAGACCGTCG‑CAGCCTCGTAAACATCTTGATTTTTGGACGGTGTTTGAGGTTTGGG‑TTccgaaagaaac                                                                                                                   >  2:314008/1‑241 (MQ=255)
                                                                                                                         aaCTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGG‑TTCGATAAGCAACgg                                                                                                                 >  1:303358/1‑250 (MQ=255)
                                                                                                                            tGGTCGGTGTCCCATCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCATATTGAt                                                                                                                                                          >  1:259421/1‑208 (MQ=255)
                                                                                                                             ggTCGGTGTCCCAACCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAATGGTGACCTGCCAGCGGCGCGTAATTATCATCAATGTTCAGTTTAATCTCTTTTTTCAGACCAAAATGTTTCAGGAAGCCATAGACCGTCG‑CGGCCTCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTAGG‑TTCGAGAAGCacagtgacc                                                                                                             >  1:180990/1‑243 (MQ=255)
                                                                                                                                      cccagcccagTTGCGCATCGCCACGTTTGTCTTCTAAAGAACCGAACAGGCCAAGCGTAATGGCGGTTGTTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCATATTGATGTTTGGGCGGTTCTTGCGGTTTCGG‑TTCGATAAGCAACGTGCCCTGGAAACCg                                                                                                    <  2:192948/251‑1 (MQ=255)
                                                                                                                                         agcccagTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCCGCGTCGCGTAATTAGCTTCAAAGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAACCATAGACCGTCC‑CGGCCTCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGGTTCGG‑TTCGTTAAGCAACGTGCCCTGGCAACCGAtt                                                                                                 >  1:164029/1‑251 (MQ=255)
                                                                                                                                             cagTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGG‑TTCGATAAGCAACGTGCCCTGGAAACCGATtttat                                                                                             <  1:8851/251‑1 (MQ=255)
                                                                                                                                                            actggtagCGTCGTCAGAATCTAACAGGCCAAGCGCAATGGCCGTTGCTAGTTCATGAGTGAAAGTGTGATCTTCCATCGTCTCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAATCCATAGACC‑TCGGCGGCATCGTAATCATATTGATGTTGGGTCGGTTCTTGCGTTTTCGG‑TGCCATAAGCAACGTGCCCTGGAAACCGATTTTATTGTTATGCTCAacca                                                                              <  2:277620/244‑1 (MQ=255)
                                                                                                                                                               gTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGGGTGACCTGCCAGGGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCCGACCAAACTGGTTCAGGAAGCCATAGGACGTCG‑CGGCATCGAAATCATATTGAAGTTTGGGCGGTTCTTGCGGTTTCGG‑TTCGATAAGGAAAGTGCCCTGGAAACCGATTTTATGTTTATGCTCAaccacc                                                                            >  1:268402/1‑250 (MQ=255)
                                                                                                                                                                ttGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGG‑TTCGCTAAGCAACGGGCCCTGGAAACCGATTTTTTGTTTATGCTCAACCACCAt                                                                          >  1:90987/1‑251 (MQ=255)
                                                                                                                                                                              aCCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGG‑TTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGc                                                            <  1:61202/251‑1 (MQ=255)
                                                                                                                                                                                 gAACAGGCCAAGCGCAATGGCGGTGGCTATTTTATGATGGAAGAAGAGACCTGCCAGCGACGCGTAATTATCTTCAATGTTCAGTTTAATATCTTTTTCCAGACCAAACTGATTCAGGAAGCCATAAGCCGTCG‑CGGCATTGTAATCATATTGATGGTTGGTGGGATCTTGGGGTTTCGGGTGCG‑TGAGCAACGTGCACTTGAGCACGATTTTATGTTTGTTCTTAaccacaacatgcataaccgggcccc                                                         >  2:193242/1‑231 (MQ=255)
                                                                                                                                                                                 gAACAGGCCAAGCGCAATGGCGGTGGCTATTTCCTGATGGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAAATGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCATTTTGATGTTTGGTCGGTTCTTGCGGGTTCGG‑TTCGATAAGCAACGTGCCCt                                                                                                            >  2:174215/1‑200 (MQ=255)
                                                                                                                                                                                 gAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGG‑TTCGATAAGCAACGTGCCCt                                                                                                            <  1:174215/200‑1 (MQ=255)
                                                                                                                                                                                                    gCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGG‑TTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACg                                      >  1:2860/1‑251 (MQ=255)
                                                                                                                                                                                                             aTTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTGCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGG‑TTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGt                             <  1:151961/251‑1 (MQ=255)
                                                                                                                                                                                                                  atgatgGAAAGAGTGACCTGCCAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGG‑TTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTAttt                         >  1:297533/1‑250 (MQ=255)
                                                                                                                                                                                                                                    tGCCAGCGTCGCGTAATAAGCTTCAATGTTCAGTTTAATCTCTTATTCCAGACCCAACTGTTTCAGGACGCAATGGACCGTCG‑CGGCATCGAAATAATATTGATGTTTGGTCGGTTCTTGCGGTTTCGC‑TTCGATAAGCAACGTGCCCAGGAAACCATTTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGCAGCAAGGCGGAATTTAACAGCTTTTAGTacccgt      >  2:262584/1‑246 (MQ=255)
                                                                                                                                                                                                                                      ccAGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGG‑TTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCa    <  1:314008/251‑1 (MQ=255)
                                                                                                                                                                                                                                        aGCGTCGCGTAATTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGG‑TTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACg  <  2:2860/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                                 
ATCATATTTATCAGTACTTTGACGACGTACTTTGGCATCGAAGTTCAGACCACCGGTGGTGAAACCGCCTGCTTTGAGAATTTCATACATCACCAGCGCATTCTCTTCCACACTGTTCGGGAACTGGTCGGTGTCCCAGCCCAGTTGCGCATCGCCACGGTTGGCGTCGACAGAACCGAACAGGCCAAGCGCAATGGCGGTGGCTATTTCATGATGGAAAGAGTGACCTGCCAGCGTCGCGTGGTTAGCTTCAATGTTCAGTTTAATCTCTTTTTCCAGACCAAACTGTTTCAGGAAGCCATAGACCGTCG‑CGGCATCGTAATCATATTGATGTTTGGTCGGTTCTTGCGGTTTCGG‑TTCGATAAGCAACGTGCCCTGGAAACCGATTTTATGTTTATGCTCAACCACCATCTGCATAAAGCGGCCCAGTTGTTCACGCTCCTGACGCAAGTCGGTATTTAACAGCGTTTCGTAACCTTCACG  >  NC_000913/3729713‑3730195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑