Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,143,377 C→T S283L (TCA→TTA)  frwC → putative PTS enzyme IIC component FrwC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,143,3770CT92.3% 73.6 / ‑0.9 26S283L (TCA→TTA) frwCputative PTS enzyme IIC component FrwC
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (10/14);  total (11/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

GATTTGCGTTGCTCAGGGTGTTTATACCGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGG  >  NC_000913/4143144‑4143615
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                              
gATTTGCGTTGCTCAGGTTGTTTATACCGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTa                                                                                                                                                                                                                                <  1:305825/250‑1 (MQ=255)
gATTTGCGTTGCTCAGGGTGTTTATACCGTGGTGGCCATCGCTGCCGTGGGTTTTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGt                                                                                                                                                                                                                                 <  1:2493/249‑1 (MQ=255)
          gCTCAGGGTGTTTATACCGTGGTGGCCATCGCTGCCGGGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCt                                                                                                                                                                                                                     <  1:314094/251‑1 (MQ=255)
                    tttATACCGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTgcg                                                                                                                                                                                                           >  1:183716/1‑251 (MQ=255)
                                gggCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCa                                                                                                                                                                                               <  1:40586/250‑1 (MQ=255)
                                 ggCCAGCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCa                                                                                                                                                                                               <  1:83911/250‑1 (MQ=255)
                                                  gtatgtggaTCCCGCCGCGGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCTGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGGGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGAtt                                                                                                                                                                             <  1:341655/243‑1 (MQ=255)
                                                        gTATCCCGCCGCTTGGGATGGGGCGGGCGACGCTGATTGGTCGTAAAAATTTCTCCGGAGAAGAGCGCGAAACCGGCAAAGCGTCACTGGTGATGGGCGGTTTTGGCGTTACTGAAGGGTCGTGTCCTTTCGCCGCGGCCGATGCTCTGCGTGTCATTCCTTCCATCATGGTCGGGTTAGTTTGTGGTGCAGTAACGGCGGCGCTGTTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGtgct                                                                                                                                                                        <  1:30812/250‑1 (MQ=255)
                                                                      gggATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAgg                                                                                                                                                         <  1:22292/251‑1 (MQ=255)
                                                                                         tGATTGGTCGTAAAAATTTCTCCGCAGAAGATCGCGAAACCGGCAAAACGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCCTGGTCGGTTCAGTGTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCTGTGCTATGCAGGCTGGGGGGGTCTGGTTGTGATGCCGGTGGTTTAAGGCAAGCTGGGTTgtattgc                                                                                                                                       >  2:64998/1‑247 (MQ=255)
                                                                                                                 cAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGATGGGGTGGTCTGATTGTGCTGCCGGTGGTTg                                                                                                                                                             >  1:185430/1‑204 (MQ=255)
                                                                                                                 aagaagaGCTCGAAACCGGCAAAGCGGCCCTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTg                                                                                                                                                             <  2:185430/203‑1 (MQ=255)
                                                                                                                        gcgcGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCCGTGCTATGCAGGCTGGGGGGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGGTATATCGCAGCTGTGGCTGTTGGaagatgggatgagcgcc                                                                                                       >  2:88839/1‑235 (MQ=255)
                                                                                                                        gcgcGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGa                                                                                                                       <  1:88839/235‑1 (MQ=255)
                                                                                                                                                       ggCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGcgcg                                                                        >  1:48151/1‑251 (MQ=255)
                                                                                                                                                                ggCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATgg                                                               <  1:52512/251‑1 (MQ=255)
                                                                                                                                                                ggCGTTACTGAAGGGGCGATTCCTTGCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGGCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATgg                                                               <  1:152158/251‑1 (MQ=255)
                                                                                                                                                                 gCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATggg                                                              >  1:109899/1‑251 (MQ=255)
                                                                                                                                                                         gAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTATGGCGCGTAAAAATGGGGCTTCGAc                                                      >  1:14496/1‑251 (MQ=255)
                                                                                                                                                                              ggCGATTCTTTGCTCCGCTTCCGAGCCGCTGCGTTTCATTCTGTCCAGCATGGTCGGTTTTGTTTGGGGTGCAGTAACTGCGTCGCTTGTCGGTGCGCAGTGCTTTGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGATCGGGCTGAAAAGTCTTGCGCGTAAAAATGTGTCTTCGACtgatg                                                 <  2:11001/251‑1 (MQ=255)
                                                                                                                                                                                             cgcTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCggtggt                                                                                                                                                               >  2:127766/1‑126 (MQ=255)
                                                                                                                                                                                             cgcTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCggtggt                                                                                                                                                               <  1:127766/126‑1 (MQ=255)
                                                                                                                                                                                                       ccGCTGCGTGTCATTCCTTCCATCATGGTCGGTTTAGTTTATGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGGAAAGTCTGGCGCGGAAAAATGGGTCTTCGACTGATGAAAAAGAAGAAGACCtggatttggg                        >  1:322043/1‑250 (MQ=255)
                                                                                                                                                                                                                     tccttccATCATGGTCGGTTCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAaattaat           <  1:64998/250‑1 (MQ=255)
                                                                                                                                                                                                                           catcatGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTACTTAATTg     >  1:231647/1‑250 (MQ=255)
                                                                                                                                                                                                                             tcatGGTCGGTTTAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAgg  >  1:201827/1‑251 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                              
GATTTGCGTTGCTCAGGGTGTTTATACCGTGGTGGCCATCGCTGCCGTGGGTATTTGTATCCCGCCGCTGGGGATGGGGCTGGCGACGCTGATTGGTCGTAAAAATTTCTCCGCAGAAGAGCGCGAAACCGGCAAAGCGGCACTGGTGATGGGCTGTGTTGGCGTTACTGAAGGGGCGATTCCTTTCGCCGCTGCCGATCCGCTGCGTGTCATTCCTTCCATCATGGTCGGTTCAGTTTGTGGTGCAGTAACTGCGGCGCTGGTCGGTGCGCAGTGCTATGCAGGCTGGGGTGGTCTGATTGTGCTGCCGGTGGTTGAAGGCAAGCTGGGTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGG  >  NC_000913/4143144‑4143615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑