Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,358,8590TC96.5% 95.1 / NA 29Y110C (TAT→TGT) puuPputrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (12/16);  minor base A (1/0);  total (13/16)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

CGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGC‑GATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATC  >  NC_000913/1358617‑1359104
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      
cGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCcagc                                                                                                                                                                                                                                                <  1:294629/251‑1 (MQ=255)
  gTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAAAGGCGACGGAGGTTTCCACCCCCACCC‑CCGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCCAAAAGAGACAATCcagc                                                                                                                                                                                                                                                >  1:49169/1‑249 (MQ=255)
       aaCGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTGCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATg                                                                                                                                                                                                                                        <  1:300483/251‑1 (MQ=255)
                 ccttttgtcAGTCCCGGAACGACCAGGAATATAAACACCACCATGTTGGAGAGGTGCACCAGTACAAACAGGGTATTGACGTTAGCGACCGGGTTGACGTGCTTCAGATTCGCGGCGGTTAAAATGTCGACGAAGGTTACCCCCCACTCCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCCCCATCAAGACGTTAATCACCGGCAAAAATAGACTATCCAGCATC‑GATGTCCAGCCg                                                                                                                                                                                                                                <  2:201013/241‑1 (MQ=255)
                 ccTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGcc                                                                                                                                                                                                                                 <  1:42873/248‑1 (MQ=255)
                   tttATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAAAGGCGACGAAGGGTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGGGGGGGGAGTGAATTTCGCCACCAAAACGTTAGACATCGGCTCAAAGAGAAAATCCAGC‑GCAGATGACCAGCCGAcc                                                                                                                                                                                                                             >  2:114958/1‑250 (MQ=255)
                                      aCCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCCGACGTGCGGGtt                                                                                                                                                                                                         <  1:2378/251‑1 (MQ=255)
                                       ccAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGTCGACGAAGGTTTCCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCCGACGTGCGGGTTa                                                                                                                                                                                                        <  1:9785/251‑1 (MQ=255)
                                                                            cAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTGCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCTGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCCGACGTGCGGTTTAATCGACTTTTGCGCGTAGGGATAGGCCGAAcc                                                                                                                                                                        <  1:261298/246‑1 (MQ=255)
                                                                                  aaaCAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGaa                                                                                                                                                             <  1:147396/251‑1 (MQ=255)
                                                                                     cAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTTCCACCC‑CACCCCACGGCGGCCCTTCCCGGAAGAGGGGGGAGAGATAGATTTTCGCCAACAAGCCGTTAATCATCGGCAAAAAGAGACAAACCAGCAGA‑GGGGACCAGACGCACATAAATCCCACGTGCGGGTTTATCGCGTTTTTCGCGTAGGGATAGGCCGAAACGGCCTCCgggaaat                                                                                                                                                          >  1:3976/1‑246 (MQ=255)
                                                                                                                               cgcgGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCacac                                                                                                                <  1:114958/251‑1 (MQ=255)
                                                                                                                                  ggcggTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcg                                                                                                             <  1:114365/251‑1 (MQ=255)
                                                                                                                                    cggTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgcc                                                                                                           >  1:253132/1‑251 (MQ=255)
                                                                                                                                                            ccacccacCCCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGAAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGa                                                                                   <  1:86053/251‑1 (MQ=255)
                                                                                                                                                               cccacaccc‑acGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACg                                                                                >  2:68157/1‑251 (MQ=255)
                                                                                                                                                                   cccccaccggcgccccTTCGGGGAAGAGGGCGGAGAGATAGTTTTCCGCCAACAAGCCGTTAACCATCGGAAAAAAGAGACAATCCAGCAGC‑GATGCCCAGCCGACCATAACCCCGACGTGCGGGTTAACCGTTTTTTGCGCGTAGGTATAGGCCGACCCGGCCCCCGGAAACTGGCGACCCAGTTTGCCGTAGCTGATAACGTTAAACAGCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGa                                                                             <  1:187460/236‑1 (MQ=255)
                                                                                                                                                                           ggcggcACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGACGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCCGCGCCAGCAAATAGGACACCGGGACGTGACCGTCGCt                                                                     >  2:269792/1‑251 (MQ=255)
                                                                                                                                                                                      cGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCgg                                                             >  1:133171/1‑248 (MQ=255)
                                                                                                                                                                                        ggAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCCGACGTGCGGTTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACa                                                        <  1:269792/251‑1 (MQ=255)
                                                                                                                                                                                                          agatTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCaaa                                       >  1:275050/1‑250 (MQ=255)
                                                                                                                                                                                                             ttttCGCCAACAAGACGTTAATCATCTGCAAAAAGAGACAATTCAGCAGC‑GATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGTCTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTAGCTAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCAAACACGCCAGCGCCAGCAATTAGGACGCCGGAAAGGGACCGCCGCTCATGCCGGAGCCAATACCAAAAGTATCACATACg                                   >  2:257035/1‑251 (MQ=255)
                                                                                                                                                                                                                          gACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAAAAAATACCAAAAGTATCAAATACGGACCTCGGCGTg                       >  2:14742/1‑250 (MQ=255)
                                                                                                                                                                                                                           cgtttAACCATCGGAAAAAAGATACAACCCGGCAGC‑GTTGCCCACCCAACCAAAAATCCTGCGTGCGTTTTAACCGTTTTTTGCCGGTGGGTATGGGCCGAACCGGCCTCCGTAACCTGGCGAACCATTTTGCCGTGTCTGATAGCGGTAATCACCACTCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACTGTCATCGGCGTgag                     <  2:253132/248‑1 (MQ=255)
                                                                                                                                                                                                                           aCGTTAATCAGCGGCAAAAAGAGACAACCCAGCAGC‑GATGACCAGCCGCCCATAAATCCGACGTGCGGGTTAATCGACTTTGGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACGGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTgag                     <  1:14742/251‑1 (MQ=255)
                                                                                                                                                                                                                                    atcGGCAAAAAGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGAGTGAGATAAGcc              >  2:301719/1‑249 (MQ=255)
                                                                                                                                                                                                                                          aaaaaGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTcc                                                                                                                                                                 >  2:197909/1‑96 (MQ=255)
                                                                                                                                                                                                                                          aaaaaGAGACAATCCAGCAGC‑GATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTcc                                                                                                                                                                 <  1:197909/96‑1 (MQ=255)
                                                                                                                                                                                                                                               gagaCAATCCAGCAGC‑GATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGGTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGCTAGGCCCGGCCCatcatc  >  1:87888/1‑250 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      
CGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCC‑ACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGC‑GATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCATC  >  NC_000913/1358617‑1359104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑