Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,169,836	A→G	L180P (CTG→CCG)	ldtC ←	L,D‑transpeptidase LdtC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,169,836	0	A	G	100.0%	87.6 / NA	27	L180P (CTG→CCG)	ldtC	L,D‑transpeptidase LdtC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/13);  total (14/13)

GGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTCGCGCG  >  NC_000913/1169590‑1170076                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 ggCTTATGTGCTTCAACCAGACTCGCACCGTTTTGTTCGGCAGAGACTGTTATCGGAGTGTTGATGATATTCCCTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTAtccggtcc                                                                                                                                                                                                                                              <  2:142095/251‑1 (MQ=255)         tACTTCAACCAGACGCGCACCGTTTGGTTCGGAAGAGACTTTTATCGGAGTGTTGCTGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGAGTTCGAACCATGAAGCAAATAAACGACGCCATAGGACGCCAGCCGAATCGCCTGATGGCCAATTGGGTTATCCGGTCCAGCCGGcc                                                                                                                                                                                                                                      >  2:170490/1‑250 (MQ=255)                         gcACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGATGATGATATTCACTTTGGTGCCTGGGGGGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGACAATGCCGAAATCGGCGTACGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCACTGGGGTATCCGGTCCAGCCGGCAATACATCAGGCacatc                                                                                                                                                                                                                      >  2:168204/1‑247 (MQ=255)                                ttGGTTCGGCAGTGATTGTTACCGGAGTGTTGATGATATTCACTTTGGTGCCGGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCTGAACGTACCCTCATGCCTATTCCGTAATCGGCGTTCGTACCAGGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCTATTcc                                                                                                                                                                                                                <  1:338299/250‑1 (MQ=255)                                             gaCTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAAc                                                                                                                                                                                                  <  1:20768/251‑1 (MQ=255)                                                  gtagcGGAGTGTTGATGATATGCACTGTGGGGCCTGGGGTGACCGGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCCTGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTGCCGGAGGCAACTCAATTCCCTGTGCTTTATAACGGgcg                                                                                                                                                                                             <  2:328495/247‑1 (MQ=255)                                                    aTCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGcgcg                                                                                                                                                                                           >  1:63038/1‑251 (MQ=255)                                                            gtTGATGATATTCACTTTGGGGCCGGGGGTGACCTGGCTAAAGAGTGTTTTGATAACGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGttt                                                                                                                                                                                    <  2:176869/250‑1 (MQ=255)                                                                                 gCCTGGGGTGACCTGGCTAAAGAGTGTTTGGATATCGTCAGCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTggg                                                                                                                                                               <  1:324347/250‑1 (MQ=255)                                                                                             cTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCAAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCAGCCACAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTCTCCGGTCCACCCGGCACTACCGCAGGCCACTCAATTCCCTGGGCTTTATAACCGGCGGGGTTGTTTGCCGTTGGCGTCCAGGTAGGGTTTGCACGTTTGt                                                                                                                                                  >  2:301466/1‑251 (MQ=255)                                                                                               ggCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTCCCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTGGGCGTCCAGGTTGGGTTTGCACGTTTGTCt                                                                                                                                                >  2:221017/1‑251 (MQ=255)                                                                                                          gtTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTCCCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACggtggt                                                                                                                                     <  1:161824/251‑1 (MQ=255)                                                                                                          gtTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGATATCCGGTCCAGCCGGCACTACCGAAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGACGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACggtggt                                                                                                                                     >  1:223711/1‑251 (MQ=255)                                                                                                                           ccGCAGACGACTACAGCCAGGACTTACCCGCATGCAAATGCCGAAATCGGAGATCGTACCATGAAGCAAATAAACGCCGCCACAAGCCCACAGACGAATCGCATGATGGCCCATTGGATTATCCGGTCCAGACGGCACTACCGCAGGCAACTCAATTCACAGTGCTTTATAACGGGCGCGAATGTTTGCGGCTAGAGTCCAGGTGGGGTGTGCACGTTTGTCTGAAACGGTGGTCACCATTGACGGTGTc                                                                                                                     >  2:283207/1‑250 (MQ=255)                                                                                                                                                    aCCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAAt                                                                                           >  1:10305/1‑251 (MQ=255)                                                                                                                                                           tGCCAATGCATAAATCGGCGTTCGGGCCATGAAGCAACTAAACGCCGCCATAGGCCGCCAGACGAATCGCATGCTGGCCCATTAGGATATCCGGTCCAGCCGGCACTACCGCAGTCAACTCCATTCCCTGTGCTTTCTAACGGGCGCGGCTCTTTGCCGTTGGCGTCCAGGTTGGTTTTGCACGCTTTTCTAAAACGGAGGTCACCATTGTCGTAGTCCTCGTGTCTCCACGTCACTACCCAATACCTAtt                                                                                    >  2:39243/1‑251 (MQ=255)                                                                                                                                                                   cggAAATCGGCGTTCGTACCATGAAGCAAAGAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATacac                                                                            <  1:261600/249‑1 (MQ=255)                                                                                                                                                                    ccaaaTCGGCGTACGTACCATGAAGCAAATAACCGCCGCCATAGGCAGCCAGACGAATCGCATGATGGCCCATTGGGTTATACGGTCCAGCCGGCACTACCGAAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCGGTTGGCGTCTAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCAATGTAGGTGTCAGCGTGTCAACACCTAACTGACAAATACCTATTGGTTACACg                                                                           >  1:78992/3‑251 (MQ=255)                                                                                                                                                                               ttCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGc                                                                                                                                                                           >  1:176998/1‑144 (MQ=255)                                                                                                                                                                               ttCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGc                                                                                                                                                                           <  2:176998/144‑1 (MQ=255)                                                                                                                                                                                  gTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGGTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGGCGGTGTCAGAGTGTCACCAACTAACTGTCCAATACCTATTGGATCCACGGTTAACGAAttttt                                                             >  2:72989/1‑251 (MQ=255)                                                                                                                                                                                     ccATGAAGCAAATAAACGCCGCCATATGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTAcc                                                          <  1:116022/251‑1 (MQ=255)                                                                                                                                                                                                   cacgacgccATAGGCCGCCAGAAGAATCGCATGATGGCCCATTGGGTTCTCCGGAACAGCCGGAAATACCGCCGGCAACTCAATGCCCTGTGCTTGATAACGGGCGCGGATGTTTGCCGTTGGCGGCCAGGTTGGGATTGCTCGTTTGTCTGAAACGGTGGTCACCATAGTCGGTGTCAGCGGCTTACCACATAACTGACCAAGACCTCTTGGGTACCCGGTTACCGAATTTTTACCCGGCgggatgtaat                                            >  2:37444/6‑244 (MQ=255)                                                                                                                                                                                                   aaCGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAAt                                            <  1:168204/251‑1 (MQ=255)                                                                                                                                                                                                                  cgccAGACGATGCGCATGATGGCCCTTTGGGTGACCCGGTCCAGCTGGCGCTACCGTAGGCAACTCAATTCCCTGTGGTTAATAACGGGCGCGGATGTTTGCCGTGTGCGGCCAGGCTGGGTTTGCTCGTCTGTCGGAAACTGTGGTCACCATTGTCGGTGTCCGCGTGTCCCCTCCTAACTGACCAATACGTATTGGATACACGGTTACCGAATTTTTACCCGGTGGGTTGTAATCGATACGCAGCTctg                             <  2:52229/251‑3 (MQ=255)                                                                                                                                                                                                                         cGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGtt                      >  1:320470/1‑251 (MQ=255)                                                                                                                                                                                                                                        cccATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACGCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTTTCAGCGTGGCACCACCTATCTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGTGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCtgc       <  1:112109/251‑3 (MQ=255)                                                                                                                                                                                                                                              gggTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTcgcgcg  <  1:3967/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 GGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTCGCGCG  >  NC_000913/1169590‑1170076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑