Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,899,195	C→T	E67K (GAA→AAA)	yieK ←	putative glucosamine‑6‑phosphate deaminase YieK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,899,195	0	C	T	100.0%	20.1 / NA	7	E67K (GAA→AAA)	yieK	putative glucosamine‑6‑phosphate deaminase YieK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/3);  total (4/3)

CTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTATCAATAGTGAGCTTCTGGATATTCTCTTCTTTGATCCCCGCAGGGGTGAAAAACAGATTACGCAGATTGGTAATCGTTACGCCTTCTCCCTCTTTGCCGCGAAATGGAATTTCATCAAAATTATAGAAATAGCAGTTATCGTACCAGGGCTTACCTTTAACCAGGGTGGTGAGGTATTCATACATGCCTTTGGGCGTGCTACCGGCGGTAATTGCCAGGTTAACACGACGCGTCTTCGACATATAACCAAGCAGATGGTGTGCCGCGACACG  >  NC_000913/3898962‑3899354                                                                                                                                                                                                                                          |                                                                                                                                                                cTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTATCAATAGTGAGCTTCTGGATATTCTCTTCTTTGATCCCCGCAGGGGTGAAAAACAGATTACGCAGATTGGTAATCGTTACGCCTTCTCCCTCTTTGCCGCGAAATGGAATTTTATCAAAATTATAGAAAt                                                                                                                                                >  1:158318/1‑251 (MQ=255)                                              aTCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTCGTTATCAATAGTGAGCTTCTGGATATTCTCTTCTTTGATCCCCGCAGGGGTGAAAAACAGATTACGCAGATTGGTAATCGTTACGCCTTCTCCCTCTTTGCCGCGAAATGGAATTTTATCAAAATTATAg                                                                                                                                                    >  2:39181/1‑202 (MQ=255)                                              aTCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTATCAATAGTGAGCTTCTGGATATTCTCTTCTTTGATCCCCGCAGGGGTGAAAAACAGATTACGCAGATTGGTAATCGTTACGCCTTCTCCCTCTTTGCCGCGAAATGGAATTTTATCAAAATTATAg                                                                                                                                                    <  1:39181/202‑1 (MQ=255)                                                                             cgTCCGCCTTACCGCACCAGTATCTGATCATGCTCGCGGTAGTTATCAATAGTAAGCTTCAGGATATTCTCTTCTTTGATCCCCGCAGGGGTAAACAACAGATTACGCAGATTGGTAATCGTTACACAATCTCCCTCTTAGCCGCGAAATGGAATTTTATCACAATTATCGCACTAGCAGTTATCGTACCAGGGCTTACCTTTAACCAGGGTGGTAAGGTATTCATCCATGCCTTTGGGCGTGCTACcggc                                                                    >  2:349104/2‑251 (MQ=255)                                                                                                   tCTGATCATGCTCGCGGTAGTTATCAATAGTGAGCTTCTGGATATTCTCTTCTTTGATCCCCGCAGGGGTGAAAAACAGATTACGCAGATTGGTAATCGTTACGCCTTCTCCCTCTTTGCCGCGAAATGGAATTTTATCAAAATTATAGAAATAGCAGTTATCGTACCAGGGCTTACCTTTAACCAGGGTGGTGAGGTATTCATACATGCCTTTGGGCGTGCTACCGGCGGTAATTGCCAGGTTAACacg                                               <  1:184254/250‑1 (MQ=255)                                                                                                                                        cTGGATATTCTCTTCTTTGATCCCCGCAGGGGTGAAAAACAGATTACGCAGATTGGTAATCGTTACGCCTTCTCCCTCTTTGCCGCGAAATGGAATTTTATCAAAATTATAGAAATAGCAGTTATCGTACCAGGGCTTACCTTTAACCAGGGTGGTGAGGTATTCATACATGCCTTTGGGCGTGCTACCGGCGGTAATTGCCAGGTTAACACCACCCGGCTTCGGCCTATAACCAAACAGATGGGGTGCcg         >  1:162211/1‑251 (MQ=255)                                                                                                                                                                      ggTGAAAAACAGATTACGCAGATTGGTAATCGTTACGCCTTCTCCCTCTTTGCCGCGAAATGGAATTTTATCAAAATTATAGAAATAGCAGTTATCGTACCAGGGCTTACCTTTAACCAGGGTGGTGAGGTATTCATACATGCCTTTGGGCGTGCTACCGGCGGTAATTGCCAGGTTAACACGACGCGTCTTCGACATATAACCAAGCAGATGGTGTGCCGCGACACg  <  1:43653/228‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                CTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTATCAATAGTGAGCTTCTGGATATTCTCTTCTTTGATCCCCGCAGGGGTGAAAAACAGATTACGCAGATTGGTAATCGTTACGCCTTCTCCCTCTTTGCCGCGAAATGGAATTTCATCAAAATTATAGAAATAGCAGTTATCGTACCAGGGCTTACCTTTAACCAGGGTGGTGAGGTATTCATACATGCCTTTGGGCGTGCTACCGGCGGTAATTGCCAGGTTAACACGACGCGTCTTCGACATATAACCAAGCAGATGGTGTGCCGCGACACG  >  NC_000913/3898962‑3899354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑