Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,712,407 G→A G176G (GGC→GGT)  yhjY ← putative outer membrane protein YhjY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,712,4070GA86.7% 31.5 / ‑0.3 15G176G (GGC→GGT) yhjYputative outer membrane protein YhjY
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (5/8);  total (6/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.78e-01

ACTATTGTTATTTAATGGTTTCAATTGTGACTGTTACGTTAAAATCTGGCGCTAACCCCCATCGTATACAGATAGTCGCTATTATTAGTGGTATTTTCTGCCTGAGTTAGCGCGGCATAGGCGGCAATATTTTGATTGAGCAACATATCAGCGCCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTG‑GTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGAGGTGGTTGTGCCATCGTAATGTAATGCCGCGACCGGGCCGGTTGTAACC  >  NC_000913/3712198‑3712629
                                                                                                                                                                                                                 |                                                                                                                                                                                                                               
aCTATTGTTATTTAATGGTTTCAATTGTGACTGTTACGTTAAAATCTGGCGCTAACCCCCATCGTATACAGATAGTCGCTATTATTAGTTGTATTTTCTGCCTGAGTTAGCGCGGCATAGGCGGCAATATTTTGATTGAGCAACATATCAGCGCCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGACCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTG‑Gtt                                                                                                                                                                                       <  1:25760/251‑1 (MQ=255)
   aTTGTTATTTACTGGTTTCAATTGTGACTGTTACGTTAAAATCTGGCGCTAACCCCCATCGTATACAGATAGTCGCTATTATTAGTGGTATTTTCTGCCTGAGTTAGCGCGGCATAGGAGGCAATCTTTTGATTGAGCAACATATCAGCGCCTACGGTGACATCCAGCCAGTTGCCGTTCTGGCTTGGCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCAAGATATTCTCGCCAAATTGCTG‑GTtat                                                                                                                                                                                     >  2:92315/1‑250 (MQ=255)
         aTTTAATGGTTTCAATTGGGACTGTTACGTTAAAATCTGGCGCTAACCCCCATCGTTTACAGATAGTCGCTATGATTAGTTGTATTTTCTGCCTGAGTTAGCGCGGCATAGGCGGCAAGATTTTGATGGAGCAACATATCAGCGCCTACGGTGACAGCCAGCCAGTTGCCGTTCTGGTGTGTCGCCGTCATCCGGCTCGGACCTGATTGCGCCTTCCAGAGATTCGCGCCAAATTGCTG‑GTTATAGCTGa                                                                                                                                                                               <  2:128349/250‑1 (MQ=255)
                      aaTTGTGACTGTTACGTTAAAATCTGGCGCTAACCCCCATCGTATACAGATAGTCGCTATTATTAGTGGTATTTTCTGCCTGAGTTAGCGCGGCATAGGCGGCAATATTTTGATTGAGCAACATATCAGCGCCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGACCTGATTGCGCCTTCCAGATATTCTCGCCACATTGCTG‑GTTATAGCTGATTTGCGCCCAGGGt                                                                                                                                                                 >  1:295057/1‑251 (MQ=255)
                            gACTGGTACGTTAAAATCTGGCGCTAACCCCCATCGTATACAGATAGTCGCTATTATTAGTGGTATTTTCTGCCTGAGTTAGCGCGGCATAGGCGGCAATATTTTGATTGAGCAACATATCAGCGCCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCTTCATCCGGCTCAGACCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTG‑TTTATAGCTGATTTGCGCCCAGGGTCGgag                                                                                                                                                            <  1:13867/250‑1 (MQ=255)
                                  tACGTTAAAATCTGGCGCTAACCCCCATCGTATACAGATAGTCGCTATTATTAGTGGTATTTTCTGCCTGAGTTAGCGCGGCATAGGCGGCAATATTTTGATTGAGCAACATATCAGCGCCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGACCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTG‑GTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGcc                                                                                                                                                      <  1:344417/250‑1 (MQ=255)
                                                 cgcTAACCCCCATCGTATACAGATAGTCGCTATTATTAGTGGTATTTTCTGCCTGAGTTAGCGCGGCATAGGCGGCAATATTTTGATTGAGCAACATATCAGCGCCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGACCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTG‑GTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAaca                                                                                                                                      <  1:322434/251‑1 (MQ=255)
                                                          ccATCGTATACAGATAGTCGCTATTATTAGTGGTATTTTCTGCCTGAGTTAGCGCGGCATAGGCGGCAATATTTTGATTGAGCAACATATCAGCGCCTACGGTGCCATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCGCAGACCTGATTGCGCCTTCCAGATATTCGCGCCAAATTGCTG‑GTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCt                                                                                                                             <  2:207665/251‑1 (MQ=255)
                                                                                                              cgcgGCATAGGCGGCAATATTTTGATTGAGCAACATATCAGCGCCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGACCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTG‑GTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGt                                                                         <  1:251106/251‑1 (MQ=255)
                                                                                                                  gcatgtgcggcAATATTTTGATTGATCAACATATCAGCGCCTTCGGGTACATCCAGCCAGTTGCCGTTCTGTTTTGTAGCCGTCATCCGGCTCAGACCTAATTGCGCTTTCCAGATATTCTCGCAAAATTG‑TGTGTTATATCTGTGTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGTGTGGTAGTACTGTCGcc                                                                     <  2:331434/245‑1 (MQ=255)
                                                                                                                     tAGGCGGCAATATTTTGATTGAGCAACATATCAGCGCCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGACCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTG‑GTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCACCACGCCAGCCTACGGAACTCACGCTGGCATGCCATAACGGATCGGTCCACGTGGTAAGACTGTCGCCaa                                                                   >  2:191274/1‑250 (MQ=255)
                                                                                                                            cAATATTTTGATTGCGCCACATATCAGCGCCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGACCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTG‑GTTATAGATGATTTGCGCCCAGGGTCGTAGATCGCAAAGCCGGGAGTCAAAAGTCCAGCCTAAGGTACACACGCTGGCATGACATCACGTCTCGGACAGCGAGGTAGAACTGTAGCCAAACTCGta                                                            >  2:315945/1‑249 (MQ=255)
                                                                                                                                      attgaGCAACATATCAGCGCCTACGGTGACATCCAGCCAGTTGCCGTGCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTG‑GTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGAgg                                                 <  2:122610/251‑1 (MQ=255)
                                                                                                                                                  atCAGCGCCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGACCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTG‑GTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGAGGTGGTTGTGCCAt                                     >  1:225609/1‑251 (MQ=255)
                                                                                                                                                                                     tCTGGTTTGTCGCCGTCATCCGGCTCAGACCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTG‑GTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGAGGTGGTTGTGCCATCGTAATGAAATGCCGCGACCGGGCCGGTTGTAAcc  >  1:216436/1‑251 (MQ=255)
                                                                                                                                                                                                                 |                                                                                                                                                                                                                               
ACTATTGTTATTTAATGGTTTCAATTGTGACTGTTACGTTAAAATCTGGCGCTAACCCCCATCGTATACAGATAGTCGCTATTATTAGTGGTATTTTCTGCCTGAGTTAGCGCGGCATAGGCGGCAATATTTTGATTGAGCAACATATCAGCGCCTACGGTGACATCCAGCCAGTTGCCGTTCTGGTTTGTCGCCGTCATCCGGCTCAGGCCTGATTGCGCCTTCCAGATATTCTCGCCAAATTGCTG‑GTTATAGCTGATTTGCGCCCAGGGTCGGAGATCGCCAAGCCGGGAGTCAACACGCCAGCCTAAGGTACTCACGCTGGCATGCCATAACGGATCGGTCAGCGTGGTAGTACTGTCGCCAAACTCGTTATACATCGAGGTGGTTGTGCCATCGTAATGTAATGCCGCGACCGGGCCGGTTGTAACC  >  NC_000913/3712198‑3712629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑