Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,292,992	A→C	intergenic (‑70/‑535)	hns ← / → tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,292,992	0	A	C	100.0%	47.0 / NA	17	intergenic (‑70/‑535)	hns/tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (9/8);  total (9/8)

CAGTTTACGAGTGCGCTCTTCAACTTCAGCAGCAGCCGCGCTTTCTTCTTCGCGACGTTCGTTAACAACAACTTCTAATTTTTCCAGCATTTCTTCCAGCGTTTCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAA  >  NC_000913/1292749‑1293230                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               cAGTTTACGAGTGCGCTCTTCAACTTCAGCAGCAGCCGCGCTTTCTTCTTCGCGACGTTCGTTAACAACAACTTCTAATTTTTCCAGCATTTCTTCCAGCGTTTCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCCATAGcc                                                                                                                                                                                                                                          >  1:316259/1‑250 (MQ=255)                           cagcagcagCCGCGCTTTCTTCTTCGCGACGTTCGTTAACAACAACTTCTAATTTTTCCAGCATTTCTTCCAGCGTTTCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCCATAGCCAGGAATGTAAGGAATTCAAAATTGTTc                                                                                                                                                                                                               >  1:3366/1‑251 (MQ=255)                             gcagcagcCGCGCTTTCTTCTTCGCGACGTTCGTTAACAACAACTTCTAATTTTTCCAGCATTTCTTCCAGCGTTTCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCCATAGCCAGGAAt                                                                                                                                                                                                                                    <  1:83958/228‑1 (MQ=255)                             gcagcagcCGCGCTTTCTTCTTCGCGACGTTCGTTAACAACAACTTCTAATTTTTCCAGCATTTCTTCCAGCGTTTCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCCATAGCCAGGAAt                                                                                                                                                                                                                                    >  2:83958/1‑228 (MQ=255)                                                                                                       ttCAAGTGTACATTCTCGTGCCTGCGCACGAAGAGTACGGATGTTGTTAAGAATTTTAAGTTCTTCGCTCATTGTAGTAATCTCAAATTTATAGTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCCATAGCCAGTAATGTAAGGAATTCAAAATTGTTCTTTATTCTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTacac                                                                                                                                    <  1:191979/250‑1 (MQ=255)                                                                                                            gtgtACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCCATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCgatga                                                                                                                              <  1:266071/251‑1 (MQ=255)                                                                                                               tACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGCATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCCATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGTTGAttt                                                                                                                           >  2:44590/1‑251 (MQ=255)                                                                                                                aCATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCCATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCATAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTc                                                                                                                          >  2:279772/1‑251 (MQ=255)                                                                                                                                                        aGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCCATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACAtt                                                                                  <  1:44590/251‑1 (MQ=255)                                                                                                                                                            ttttAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCCATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTAt                                                                              >  1:248677/1‑251 (MQ=255)                                                                                                                                                                                gtagtaATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCCATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGc                                                                                                                                                      <  1:40458/159‑1 (MQ=255)                                                                                                                                                                                gtagtaATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCCATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGc                                                                                                                                                      >  2:40458/1‑159 (MQ=255)                                                                                                                                                                                          caagctgatagtGGGTTGGTTTGTTGAGGTAATAATAGAGACTTAAATTCAGTTGTGCCATAGCAAGAAATGTAAGTAATTTAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAAGTAGCCAGAAAAGCCGCGGCATATAGCCCTATATACACCGATGATTTCGTAGCACGTGATGTTAAACCTTCCTGATTCTTGTCACATTTTATGGGGAGATGATCGTAGGCTGACGTCCtc                                                  <  2:149358/238‑2 (MQ=255)                                                                                                                                                                                                         gtggtgTGTTTAGGTAATAATAGAGCATTAAAGTCAGGTGTGCCAGAGAAAGTAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCATTAAATATCTTTTCATAAAATGAGTCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATGTCGCAGCACGTGATGTTAAAATGTCCTGATTCATGTCTCATTTTATTGGGAGATTATCGTAGTCTGACGACCTTTCAGGCGCCTGTATTAg                                 <  2:162502/251‑1 (MQ=255)                                                                                                                                                                                                                     ggTAATAATAGAGCCTTAAATTCAGTTGTGCCATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACg                      >  1:338911/1‑250 (MQ=255)                                                                                                                                                                                                                     ggTAATAATAGAGCCTTAAATTCAGTTGCGCCATAGCCAGGAATGTAAGGAATTCAACATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATCGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCCTGTCACATTTTATGGGGAGATTATCGAAGGCTGAAGACCTTTCAGTCTTCTGTATTAGATGTGTTTACg                      >  2:290621/1‑250 (MQ=255)                                                                                                                                                                                                                                        aTTCAGTTGTGCCATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGaa  <  2:4978/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               CAGTTTACGAGTGCGCTCTTCAACTTCAGCAGCAGCCGCGCTTTCTTCTTCGCGACGTTCGTTAACAACAACTTCTAATTTTTCCAGCATTTCTTCCAGCGTTTCAAGTGTACATTCTCTTGCCTGCGCACGAAGAGTACGGATGTTGTTCAGAATTTTAAGTGCTTCGCTCATTGTAGTAATCTCAAACTTATATTGGGGTGGTTTGTTGAGGTAATAATAGAGCCTTAAATTCAGTTGTGCAATAGCCAGGAATGTAAGGAATTCAAAATTGTTCTTTATTTTGTGCCGCCAATAAATATCTTTTCATAAAATTAGCCAGAAAAGACGCGGCATATAGCCCTATTTACACCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAA  >  NC_000913/1292749‑1293230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑