Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,157,902 G→T V12F (GTC→TTC)  ptsG → glucose‑specific PTS enzyme IIBC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,157,9020GT90.0% 61.2 / ‑1.4 30V12F (GTC→TTC) ptsGglucose‑specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (16/11);  total (17/13)
Fisher's exact test for biased strand distribution p-value = 5.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

TATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCAT  >  NC_000913/1157661‑1158131
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                       
tatTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACCCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGTTCGGTAAAt                                                                                                                                                                                                                               >  1:127441/1‑251 (MQ=255)
         atatGTTTTGTCAAAATGTGCAACTTCTCCAATTATCTTCCTTTTCCCCTTGATAGCCGTCAAACAAATCGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTACCTAATGTTTAAGAATGCATTTGCTAACCTGCAAAAGTTCGGTAAATCGCTGAtgc                                                                                                                                                                                                                      >  1:288782/1‑251 (MQ=255)
                        aTGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCAAGCGTGAGAACGTAAAAAAAGAACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGTTCGGTAAATGGCTGATGCTGCCGGTATCCGTAc                                                                                                                                                                                                       >  1:151635/1‑251 (MQ=255)
                             cAACATCTCCAATGATCGGAAGTTGAAACGTGATAGCCGTCAACCAAATTGGCAATGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCACCATTGCCACGCGAGAGACCGTAACAAAAGCACCCATACACCGGAGCACTCACACTTATGTTTAAGACTGAATTTGTTACCCTGACAAAGTTCGGTAAATATCTTTTCCGCCCGGTATCCGTACTGCCt                                                                                                                                                                                                  >  2:189856/1‑251 (MQ=255)
                               aCTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGTTCGGTAAATCGCTGATGCTGCCGGTATc                                                                                                                                                                                                            <  1:29154/239‑1 (MQ=255)
                               aCTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGTTCGGTAAATCGCTGATGCTGCCGGTATc                                                                                                                                                                                                            >  2:29154/1‑239 (MQ=255)
                                 ttCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGTTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCg                                                                                                                                                                                              >  1:109680/1‑251 (MQ=255)
                                      cAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGACTTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGCGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACACAGGAGCCCTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAACGTTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGt                                                                                                                                                                                         >  2:284900/1‑251 (MQ=255)
                                                     gaaacgtgatatactacaaacaaaTTTGCACGGAATTTTTTTACTCTGTGAAATAAATAAAGGGTGCTTAGATACCCTGTACAAGGCGAGGCTCTCCCCCCTGGCCACGCGTGAGAACGTAAAAAAAGCACCCATACGCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGTTCGGTAAATCTCTGAGGCGGTCGGTATCCGTACTGCCTATCGCAGGTAGTCTGCTGGGCGTc                                                                                                                                                                         <  2:249160/235‑1 (MQ=255)
                                                         cGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGTTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGtt                                                                                                                                                                     >  1:231568/1‑251 (MQ=255)
                                                            gATAGCCGTCAAACAAATTTGCACTGAATTATTTTACTCGGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGTTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTcc                                                                                                                                                                   <  2:72451/250‑1 (MQ=255)
                                                                   gTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGAAAAAGTTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGAAGGTAGTCTGCTGGGCGTCGGTTCCGCGAAttt                                                                                                                                                           >  2:235512/1‑251 (MQ=255)
                                                                                    tGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGTTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCccgccg                                                                                                                                          >  1:262058/1‑251 (MQ=255)
                                                                                     gAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGTTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTct                                                                                                                                                                                    <  2:340212/209‑1 (MQ=255)
                                                                                     gAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGTTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTct                                                                                                                                                                                    >  1:340212/1‑209 (MQ=255)
                                                                                                                         tAGATCCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATTCTCAGGAGCACTCCTAATTATGTTTAAGAATCCGTTTGCTAACCTGCAAAAGTTCGGTAAATCGCTGATGCTGCCGGTATCCGTCCTGCCTATCGCAGGTATTCTGCTGGGCGGCGGTTCCGCGAATTTCAGCTGGTTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTcc                                                                                                       <  1:301258/249‑1 (MQ=255)
                                                                                                                                    tACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGTTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAAc                                                                                           >  1:38169/1‑250 (MQ=255)
                                                                                                                                            gaggccccccccccTTGCCACGGGTGAAAACTTAAAAAAAGCACCAATCCTCAGGAGCCCCCTAAATTATGTTAAAGAATGCATTTGTTAACCTGCAAAAGGTCGTTAAATCGCGGATGCTGCGGGTATCCGTCCTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGATTTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTg                                                                                  <  1:89939/243‑1 (MQ=255)
                                                                                                                                            gAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGATAACATGCAAACGTTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCACAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCAGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCgggctccgcttttccaaccatccccct                                                                                  >  2:33475/1‑226 (MQ=255)
                                                                                                                                             agcctccccccccTTGCAACGCGTGAGAACGAAAAAACCGCACCCATACACAGTTGCTCTCTCAATTATGTTTAAGAATGCTTTTTCTAACCTGAAAAATTGGGGTAAATCGCTGATGCGGCCGGTTTCCTGACTGCTTTCCGCTGGCTTTCTGTGGGGGTTCGGTTCCGTGAATTTCAGCTGGCTGCCCGCCGTTGTATCGAATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGa                                                                                 <  1:203456/244‑1 (MQ=255)
                                                                                                                                                             ccACGCGTGAGAATGTAAAAAAGGCCCCCATACTCAGGAGCACTCTCAATTTTGTTTAAGAATGCATTTGCTACCCTGCAAAAGTTCGGTAAATCTCGGATGCTGCCGGTATCCGTTCTGCCTACCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGATTTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGgtgt                                                                 <  1:235512/251‑1 (MQ=255)
                                                                                                                                                                                  aaGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATTGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACCGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATa                                             >  1:128785/1‑250 (MQ=255)
                                                                                                                                                                                          ctACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGTTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGGCTTTTCAAACATGCCACTCATTTTTGCGATAGGTGGCGCCCTAGGCTTTAACAATAAAGGTGGCg                                    >  1:303830/2‑251 (MQ=255)
                                                                                                                                                                                          aTACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGTTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTTCAAACATGCCACTGGATTTTGCGATCGGTGGCCCCCTTGGCTTTACCAATAACGATAGCg                                    >  1:303819/1‑251 (MQ=255)
                                                                                                                                                                                          aTACCCAGGACCACTCTCAATTATGTTTAAGATGCCTTTTGCTAACCTGCAAAATTGCGGTAAATCGCTGATGCGGCCGGTATCCGTACTGCTTACCGCAGGTTTTCTGCTGGGCGTGGGTCCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCg                                    <  1:239217/251‑1 (MQ=255)
                                                                                                                                                                                               cAGGAGCACTCTCAATTATTGTTAAGAATGCATTTGATAACCTGCAAAAGTTCGGAAAATCGCTGATGCTGCCGGTTTCCTTCCTGCCTATCGCAGGTATTCTGCTGGGCGTGGTTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTAt                                 <  1:276057/249‑1 (MQ=255)
                                                                                                                                                                                                      aCTCTAAATTATTTTTAAGAAGGCATTTGCTAACCTGCAAAGGTCCAGTAAACCGCTGTTGCTGCCGGTTTCCGTACTGCCTACCGCAGGTATTCTGCTGGGGGTCGGTTCCGCGAATTTCAGTTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTgg                        <  1:112178/251‑1 (MQ=255)
                                                                                                                                                                                                       ctctCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGTTCGGTAAACCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATg                                                                                        <  1:65075/186‑1 (MQ=255)
                                                                                                                                                                                                               tATGTTTAAGAATGTATTTGCTAACCTGCAAAAGTTCGGTAAACCGCTGATGCGGCCGTTATCCGTACGTCCTATCGCAGTTATTCTGCTGGGCGTCGGTTCCGCGATTTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAgtt                <  1:287897/250‑1 (MQ=255)
                                                                                                                                                                                                                            gCATTTGCTAACCTGCAAAAGTTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTTCAAACATGCCACTGTTTTTTGCGATCGGGGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTTTTGCCTAAGGcat  >  1:75450/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                       
TATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTTAGATGCCCTGTACACGGCGAGGCTCTCCCCCCTTGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCAT  >  NC_000913/1157661‑1158131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: