Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,223,6380TC94.4% 44.9 / ‑3.4 18intergenic (‑10/‑190)iclR/metHDNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (1/16);  total (1/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.53e-01

AGTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTC  >  NC_000913/4223393‑4223876
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              
aGTTCGCCATACTGACGGACGAAACGCTGCTGGTGCAGCGTTGTTAGCAGGCGGTGGGTCTTGGAAGTGGGTAACCCGGTTTGTTGCGCCAGTTCCGTGAGTGCTACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGTCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCTCGTTTCGCGGGAATGGGTGCGTCCATGACAGTCACcttttt                                                                                                                                                                                                                                           <  2:151490/251‑1 (MQ=255)
   ccgcctgcctggcggaagAAACCCTGCTTTTGCAATGTGGTTAGCAGGCTGTGGGTCGTGTAAGTGGGTAACCCGGCTTGGTGCGCCAGTTCCGTGAGGGCCACACTTCCATTGTATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAGCCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTg                                                                                                                                                                                                                                        <  2:270188/234‑1 (MQ=255)
              ccgcccGAAACCCTGTGTGTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGTTTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGaaa                                                                                                                                                                                                                             <  2:274047/246‑1 (MQ=255)
                                   gCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGTTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGtt                                                                                                                                                                                                         <  2:68030/251‑1 (MQ=255)
                                    cATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATGTTCATTTTTATTGTTa                                                                                                                                                                                                        <  1:293229/251‑1 (MQ=255)
                                        gTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCGCGTTTCGCGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTaa                                                                                                                                                                                                   <  1:57538/251‑1 (MQ=255)
                                                            cGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactgaactg                                                                                                                                                                                <  1:325428/251‑1 (MQ=255)
                                                                     gggTACCCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCTGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATg                                                                                                                                                                        <  1:188988/250‑1 (MQ=255)
                                                                                                     gTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCgtggt                                                                                                                                       <  1:316923/251‑1 (MQ=255)
                                                                                                           cacTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGc                                                                                                                                 <  1:60738/251‑1 (MQ=255)
                                                                                                                  aTTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCGTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactgaa                                                                                                                                                                                   <  2:119357/194‑1 (MQ=255)
                                                                                                                          cGGCAAGCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTTCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCGTTTGCCACAGATg                                                                                                                   <  2:15684/250‑1 (MQ=255)
                                                                                                                                tCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAAtcattttcattt                                                                                                                                                                                                                 <  2:206005/150‑1 (MQ=255)
                                                                                                                                        aGTAATTTCAGGCCTCGTGTTAAAGTCTGATCCTTTCCTGTCGCTGGTGCGGTGTCTACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTTTAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCTATGAGTTAATGTTGAACAAATCTCATTTTGCGTGTTGGTCGCTTTTACCACAGATGCGTTTATTCCAGTAt                                                                                                    <  1:238516/251‑1 (MQ=255)
                                                                                                                                                             aaaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTttatta                                                                                <  1:130991/250‑1 (MQ=255)
                                                                                                                                                                                      gTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCa                                                      <  1:170664/251‑1 (MQ=255)
                                                                                                                                                                                                                        cgcgGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtgcgtgcg                     <  1:231300/250‑1 (MQ=255)
                                                                                                                                                                                                                                     tcgtacATGATAGTCTACTTTTTCTGTATCTTGTAAATTAATTTCATGTTTATTTGTAGCTAATTCAATAGTTTATGAACTGAACCGATGAGGTAATGTTGAACAAATCTCATGTTGCGTGGTTGTTGCTTTTACCCCATCTGCGTGTATGCCAGTATGGTTTGGTGAATTGTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGCGATCAGCAAAGTGGAACAACTGCGTTCGCAGTTAAATGAAc        <  2:182829/245‑1 (MQ=255)
                                                                                                                                                                                                                                          cATGGCAGTCCCCTTTTTCTGTATCGTGGAAATCATTTTCCTTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATACGATGAGTTAATATTTAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATATGGGTTGAGCGTGTCGGGAGCAAGGGTGCGCAGCAAAGAGGTACAACTGCGTGCGCAGTTCATTGAACGTaatc  >  2:99547/1‑248 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              
AGTTCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTC  >  NC_000913/4223393‑4223876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: