Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,553,630	C→A	G70V (GGT→GTT)	adhP ←	ethanol dehydrogenase/alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,553,630	0	C	A	100.0%	83.2 / NA	29	G70V (GGT→GTT)	adhP	ethanol dehydrogenase/alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/13);  total (16/13)

TGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACC‑ACAACACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGG  >  NC_000913/1553391‑1553874                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      tggtgACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACAACTGGACCCACt                                                                                                                                                                                                                                            >  1:305820/1‑251 (MQ=255)      ccTCCCGCACAGGTAAGGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCTTACTCGGCGCCCACGATGCACCCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCTCTGTTACAGTATTCGCAATGACCGCATCCGTCGTAGAACCACGCCACGCTGGCACGATCGCCTTGTTTTAATGAGGTGACAACTGGACCCACTTCTGc                                                                                                                                                                                                                                       <  1:89288/250‑1 (MQ=255)            gCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACAGCGTAATCGGCGACCACGATGCACTCTTCAGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGACACGCTGGCACGATCGCCTGGTTTTAATGAGGCGACAACTGGACCCACTTCTGCcaccac                                                                                                                                                                                                                                 >  1:312480/1‑251 (MQ=255)               caGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACAACTGGACCCACTTCTGCCACCACAcc                                                                                                                                                                                                                              >  1:62909/1‑251 (MQ=255)                       gctgctGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACAACTGGACCCACTTCTGCCACCACACCGATGCCtt                                                                                                                                                                                                                      <  1:238331/251‑1 (MQ=255)                                        aGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCAGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTCGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACAACTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACg                                                                                                                                                                                                     >  1:233013/1‑251 (MQ=255)                                                              tACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACAACTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGc                                                                                                                                                                               >  1:136312/1‑251 (MQ=255)                                                                     tAATCGGCGACCACGCTGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACAACTGGACCCACTTCTGCCACCACACCAATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAATTCGCCATTCtt                                                                                                                                                                        >  2:93303/1‑251 (MQ=255)                                                                     tAATCGGCGACCACGATGCACTCTTCTGCCATCCAGACAACAACGCTGTATCCGGCATTTTTAACTGCACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTAGCAATAACCTCATCCTTCGTAGAACCACGCCACGATGGCACGATCGCCTGGTTTTAATAAGGTGACAAAAGGACCCTCTTCTGCCACCCCCACGCTGCCTTCATGGCCCAGAATTACGACGGTTTTGTGACCAAAATCGGCATTCtt                                                                                                                                                                        >  2:110581/1‑251 (MQ=255)                                                                                 aCGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACAACTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATc                                                                                                                                                            <  1:315282/251‑1 (MQ=255)                                                                                           ctTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACAACTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGAc                                                                                                                                                    <  1:159666/249‑1 (MQ=255)                                                                                                  catcccgccatcAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACAACTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATAcacc‑ac                                                                                                                                          >  2:56491/1‑251 (MQ=255)                                                                                                                cGCTGTATCCGGCATTTTCAACTGACCGCCAGAGCGTTTCGCTAGCACTGTTACACCATTCCCAATGACCGCATCCCGCGTTGAACAACGCCACGCTGGCACGATCGCCTGGTTGTAATGAGGTCATAACTGGACCCCCTTCTACCACCACACCGATACCTTCATGGCACAGAATTACGCCGGTTTTGTCACCAAAATCGCCCTTCGTAACATGAAGAACGATCTAAAATACACC‑ACAACACTCCAttttt                                                                                                                            >  2:121206/1‑250 (MQ=255)                                                                                                                 gCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACAACTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACC‑ACAACACTCCATTTTc                                                                                                                            >  1:7117/1‑250 (MQ=255)                                                                                                                    ggaTCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACAACTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACC‑ACAACACTCCATTTTcagc                                                                                                                         <  1:162175/248‑1 (MQ=255)                                                                                                                                     cTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACAACTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCa                                                                                                                                                                                         <  1:325445/170‑1 (MQ=255)                                                                                                                                     cTGAACGGCAGAGCGTTTCGTTACCAATGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAAACACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACAACTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCa                                                                                                                                                                                         >  2:325445/1‑170 (MQ=255)                                                                                                                                                        gttgcccatggtacagatttCGCAATGACCGCAGCCTTCGTAGAACCACGCCACGCTGGCACGATCGCATGGTTCTAATGAGGTGACAACTGGACCCACGTCTGACACCACACCGATGCCTTCATGGCACAGAATTACGCCTGTTTTGTCACCAAATGCGCCATTCTTAACATGAAGGTCGGGATGACATACACA‑ACAACATTCCATTTTTAGCAGGGTTTCTCCATGTTTCAGTGAGCGCAGTGTTTtat                                                                                    <  2:174175/233‑1 (MQ=255)                                                                                                                                                          tACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACAACTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACC‑ACAACACTCCATTTTCAGCAGGGCTTCGCCATgagatcggaagagcacacgtcgaact                                                                                  >  1:121558/1‑226 (MQ=255)                                                                                                                                                          tACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACAACTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACC‑ACAACACTCCAGTTTCAGCAGGGCTTCGCCATg                                                                                                           <  2:121558/226‑1 (MQ=255)                                                                                                                                                          tACCACTGTTACAGTATTCGCAATGACCGCATCCTTAGTAGAACCACGCCACGCTGGCCCGATCGCCTGGTATTACTGCGGTGACAACTGGCCCCACTTCGTCCACCACACCGATGCCTTCATCGCCCAGAATTAAGCCGGTTTTGTCACAGAAATCTCCATTCTTAACATACAGATAGGTATGATATACACCGACA‑CACTCCATTTTCAGCAGTGCTTCTCCATGTTTCCATGAGCGCAGGGTTTtaga                                                                                   >  2:14972/1‑248 (MQ=255)                                                                                                                                                                        tATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACAACTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACC‑ACAACACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACatg                                                                    >  1:266122/1‑251 (MQ=255)                                                                                                                                                                                        aTCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACAACTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACC‑ACAACACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTaaca                                                    <  1:93303/251‑1 (MQ=255)                                                                                                                                                                                                 aGAACCACGCCACGCTGGCACGTTCGCCTGGTTTTAATGAGGTGACAACTGGACCCCCTTCTGCCACCACACCGAGGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACC‑ACAACACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCTTAACAACTGCAGcc                                           <  2:227633/251‑1 (MQ=255)                                                                                                                                                                                                          ccacgcTGGCACGATCGCCTGGTTTTAATGAGGTGACAACTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACC‑ACAACACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGtt                                  <  2:266122/251‑1 (MQ=255)                                                                                                                                                                                                                    aCGATCGCCTGGTTTTAATGAGGTGACAACTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACC‑ACAACACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTc                        <  1:211542/251‑1 (MQ=255)                                                                                                                                                                                                                       aTCGCCTGGTTTTAATGAGGTGACAACTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACC‑ACAACACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGga                     <  1:56491/251‑1 (MQ=255)                                                                                                                                                                                                                               gTTTTAATGAGGTGCCAACTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACC‑ACAACACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGTTGATGTTc             >  1:284079/1‑251 (MQ=255)                                                                                                                                                                                                                                          gTGACAACTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACC‑ACAACACTCCATTTTAAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAgg  >  1:342309/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      TGGTGACTCCCGCACAGGTAATGCTGCTGGCCGCCGCCGAGTCCAGACCATCTGGCACTTTTACCGCGTAATCGGCGACCACGATGCACTCTTCCGCCATCCCGCCATCAACGCTGTATCCGGCATTTTTAACTGAACGGCAGAGCGTTTCGTTACCACTGTTACAGTATTCGCAATGACCGCATCCTTCGTAGAACCACGCCACGCTGGCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACC‑ACAACACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGG  >  NC_000913/1553391‑1553874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑