Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	284,535	T→G	A445A (GCT→GCG)	yagF →	D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	284,535	0	T	G	100.0%	173.3 / NA	54	A445A (GCT→GCG)	yagF	D‑xylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (22/32);  total (22/32)

CGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGAC  >  NC_000913/284289‑284780                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       cggcgTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCgg                                                                                                                                                                                                                                                    >  1:141898/1‑251 (MQ=255)     gTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAgg                                                                                                                                                                                                                                                >  1:77999/1‑251 (MQ=255)      ggCCGGAGGTGATGCTCCCCCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGt                                                                                                                                                                                                                                               <  1:162620/250‑1 (MQ=255)                gATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTggtcc                                                                                                                                                                                                                                     >  1:9663/1‑247 (MQ=255)                           tGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGCCGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCg                                                                                                                                                                                                                         >  1:137348/1‑251 (MQ=255)                           ggcgcGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCg                                                                                                                                                                                                                         <  1:317147/250‑1 (MQ=255)                            gcgcGACCTCGGCCGGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGc                                                                                                                                                                                                                          <  1:133745/249‑1 (MQ=255)                              gcgACCTCGGCCGGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATc                                                                                                                                                                                                                      <  1:252492/251‑1 (MQ=255)                                            ctgcATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCggtggg                                                                                                                                                                                                        >  1:331801/1‑250 (MQ=255)                                                                           aGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGACTGCGCGAGCAGGACGGCGTAGCGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAACGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGA‑CAAGGGCCACGGCGATCGACCCGTCGGAGGAGGGCGACGATggaggtaacccccccacccgc                                                                                                                                                                         >  2:203137/1‑232 (MQ=255)                                                                                   ggcgagacctgtgcggggtggCAGGTGTCCGGGCGCGGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCGGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGCCCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCcgggtgcg                                                                                                                                                                 <  2:237882/235‑1 (MQ=255)                                                                                               gAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGtctc                                                                                                                                                     >  1:44501/1‑251 (MQ=255)                                                                                                     tggCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGGGCGGGTGGTTGTCTCGGaa                                                                                                                                                 >  2:175504/1‑249 (MQ=255)                                                                                                     gggCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGaa                                                                                                                                                 <  1:175504/248‑1 (MQ=255)                                                                                                        cAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAgcgc                                                                                                                                             >  1:263776/1‑250 (MQ=255)                                                                                                         aGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCag                                                                                                                                           <  1:251164/251‑1 (MQ=255)                                                                                                                  ggcgcCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaag                                                                                                                                  <  1:337897/250‑1 (MQ=255)                                                                                                                       cgtgcgcgcTCCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTGCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaaggcgat                                                                                                                             <  1:200069/248‑1 (MQ=255)                                                                                                                          gcgcgcTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTGGGGCCGGACGGC‑GTGGTCCGGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCGCGACGGTCTGCGTCCCGGCGGGCAACAGCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAggcgatcaaggcgatcaa                                                                                                                          <  2:77999/251‑1 (MQ=255)                                                                                                                           ggcgcTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGCAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGTCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCAGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGTGATCGACCCGTCGGTGGTGGGTGAAGAGGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGgcgatcaaggcgatcaag                                                                                                                         <  1:311381/250‑1 (MQ=255)                                                                                                                           ggcgattCCGCCATGGCCTGCGGGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCGGCTGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCAGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑ACACGGCGATCGACCCGTCGGTGGTGGGTGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAggcgatcaaggcgatcaat                                                                                                                         <  1:311382/246‑2 (MQ=255)                                                                                                                              gcTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGTTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCgg                                                                                                                      <  1:22196/251‑1 (MQ=255)                                                                                                                               cTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCcggcgatcaaggcgatccaaggtc                                                                                                                     >  2:267208/1‑244 (MQ=255)                                                                                                                               cTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAggcgatcaaggcgatcaa                                                                                                                          <  1:267208/246‑1 (MQ=255)                                                                                                                                        gTGCCTGCGGGAGCAGGTCGGCGGAGTGCCGGGTGCCTGTG‑GCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCGCGACGGTCGGCTTCCCGACGGGCAACCTCGCGCCGGAAGTTTCGGTGAGCAAGG‑CCACGGCGATCGGCGCGTCGGTGGTGGGCGAAGATGGCGTATACCACCGCACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATtg                                                                                                            <  2:330351/251‑1 (MQ=255)                                                                                                                                             ggcgcgggtgggaaggCGTAGAGCCGGATGGC‑GGGAACCGGCCGCCGGAGAATGCAAAAGCGAAAGGGCTGCCCGCTACGGTCTGCGTCCCGACGGGCAACGTCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGAGCCGTCGGTGGTGGGCGAAGAGGGCGTATACCACCACACCGGCCGGGGGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGAAtgtgtag                                                                                                       <  2:134135/237‑4 (MQ=255)                                                                                                                                                 cgAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCg                                                                                                   <  1:261125/251‑1 (MQ=255)                                                                                                                                                         gcggggTAGAGCCGGACGAC‑GGGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGAGCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCAtg                                                                                           <  2:328528/246‑1 (MQ=255)                                                                                                                                                          cGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCAtgg                                                                                          >  1:250110/1‑251 (MQ=255)                                                                                                                                                            gCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGTGATATCAtggtg                                                                                        <  1:205835/251‑1 (MQ=255)                                                                                                                                                             ggTAGAGCCGGAGGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCGGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGAGCAAGG‑CCACGGCGATCGACTCGTCGGTGGTGGGCGAAGATGGCGTAGACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGTAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCAtggtgg                                                                                       <  1:34464/250‑1 (MQ=255)                                                                                                                                                                  cgCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGAGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGCTCACGG‑CCACGGCGATCGACCCGTCGGTGGAGGGCGAAGCTGGCGGATACCACACCACCGGCCGGGGGCGGGGGGTTGTCTCGGCAGCGACGGCGAGCAAGGCGATCAAGCGGGAAGGGATtgtggcgggcggtcacatgggggtggtt                                                                                  >  2:16231/2‑227 (MQ=255)                                                                                                                                                                       gATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGCGATCAAGG‑CCACGGCGATCGACCCGTCGGAGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATAAAGCGGGAAGAGCTTGTGCAGGGCGATATCAGGGTGGTGATcggcgg                                                                             >  1:124026/1‑251 (MQ=255)                                                                                                                                                                              gTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGcc                                                                       >  1:229583/1‑251 (MQ=255)                                                                                                                                                                                  gccTGCCGCCGTAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCTGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCCCCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGTAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTcc                                                                   <  1:17507/250‑1 (MQ=255)                                                                                                                                                                                            ggAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACAACGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGGGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCACGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTccggcaccagcc                                                         >  1:15027/1‑247 (MQ=255)                                                                                                                                                                                               aacgggcaaaGCGGAAGGGCGGACCTCGTCGGTCCGCTTTCCGGTGGGCAACACCTCGCCGGAAGGGTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGGGGTGGGCGAAGAGGGCGTATACCACCACACCGGCCGGGTGCGTGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCTTgg                                                      <  2:224934/244‑1 (MQ=255)                                                                                                                                                                                                    cAAAAGCGAAAGGGCTGACCGCGACGGTCTGCTTCCCGACGGGCAACAGCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAagag                                                 <  2:29497/251‑1 (MQ=255)                                                                                                                                                                                                            aaaGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGGGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGa                                                     >  2:167824/1‑239 (MQ=255)                                                                                                                                                                                                            aaaGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGa                                                     <  1:167824/239‑1 (MQ=255)                                                                                                                                                                                                            aaaGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCa                                         <  1:286382/251‑1 (MQ=255)                                                                                                                                                                                                            aaaGGGCTGACCTCGACGGGCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATGAAGG‑CCACGGCGCTCGCCCCGTCGGTGGTGGGCGAATATGGCGTATACCACCGCACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCa                                         <  2:183636/251‑1 (MQ=255)                                                                                                                                                                                                               gggCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCCTGGAAGCGACCTACCAGcc                                      >  1:62763/1‑250 (MQ=255)                                                                                                                                                                                                               gggCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGGCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCt                                      <  1:14477/251‑1 (MQ=255)                                                                                                                                                                                                                    gACCTTGACGGTCCGCTTCCCGACGGGCAACATCGCGGCGGAAGGTTCGGTGATCAAGG‑CCACGGCGAACGACCCGTCGGTGGTGGGCGAAGATGGCGTATCCCCCCACACCGGCCGGGGGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCt                                 <  2:297400/251‑1 (MQ=255)                                                                                                                                                                                                                                 tGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCCTGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGc                     >  1:143100/1‑250 (MQ=255)                                                                                                                                                                                                                                   gagctcGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGACCGACCCGTCGGTGGTGGGCGAAGATGGCGGATACCACCACACCGGCCGGGTGCGGGTTTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGACCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCata                  <  1:228114/246‑1 (MQ=255)                                                                                                                                                                                                                                        cgactggaaaCATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGGGGGCGAAGATGGCGTATACCACCCCACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGGGATCGGCGGCGGGACGGCCGGCACCGGCGTGGAGGAGACCTACCAGCTCACCTCCCCGCTAAAGcctg                  >  2:228114/9‑243 (MQ=255)                                                                                                                                                                                                                                          aCGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTg           <  1:135524/251‑1 (MQ=255)                                                                                                                                                                                                                                            gggCAACATGGGGCCGGAAGGGTCGGTGATCAAGG‑CCACGGCTACCGACCCGTCGGGGGTGGGCGAAGATGGAGTAGACCCCCACACCGGCCGGGTGCGGGTGTGTGGCTCGGAAGCGCAGGCGAGCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCACACCTCCGCGCTAAAGCATAtcgcttggg         <  2:62763/251‑8 (MQ=255)                                                                                                                                                                                                                                             ggCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTgggg        <  1:151785/251‑1 (MQ=255)                                                                                                                                                                                                                                              gCAACATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTCAAGCATATCTCGTGGGGc       >  1:143214/1‑251 (MQ=255)                                                                                                                                                                                                                                                  cATCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCa      >  1:28297/1‑248 (MQ=255)                                                                                                                                                                                                                                                   aTCGCGCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCATAGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGAc  >  1:213975/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       CGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGAC‑GTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGG‑CCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGAC  >  NC_000913/284289‑284780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑