Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,769,447	G→A	E35K (GAA→AAA)	yibV →	PF15596 family protein YibV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,769,447	0	G	A	85.0%	44.9 / 2.9	20	E35K (GAA→AAA)	yibV	PF15596 family protein YibV
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (9/8);  total (10/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

AGATAGCACATTAACAGTTACGGACTAAAAAATATGGCAAGGTTATTAACAATTGTATTTTTTTCTCTATTTCTGCGAGTGCAGTTACTGCAAGACAATTTCAGAGAGAGTTAGATTTGGCAGACAAAGCAATATTATGGGCACTAATATCAGCCAGTACTAAAGAGGGACGAAAAGCGTGTTCACTAAGCTATTTTGCCTGTAAGGCCGCAGAGGCTGAACTCGGACTGGCATATATGGCTGCGAATGATAACAAGGAATTTCTCACATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAA  >  NC_000913/3769229‑3769654                                                                                                                                                                                                                           |                                                                                                                                                                                                                aGATAGCACATTAACAGTTACGGACTAAAAAATATGGCAAGGTTATTAACAATTGTATTTTTTTCTCTATTTCTGCGAGTGCAGTTACTGCAAGACAATTTCAGAGAGAGTTAGATTTGGCAGACAAAGCAATATTATGGGCACTAATATCAGCCAGTACTAAAGAGGGACGAAAAGCGTGTTCACTAAGCTATTTTGCCTGTAAGGCCGCAGAGGCTGAACTCGGACTGGCATATATGGCTGCGAATGAt                                                                                                                                                                                 >  1:137113/1‑251 (MQ=255)  gATAGCACATTAACAGTTACGGACTAAAAAATATGGCAAGGTTATTAACAATTGTATTTTTTTCTCTATTTCTGCGAGTGCAGTTACTGCAAGACAATTTCAGAGAGAGTTAGATTTGGCAGACAAAGCAATATTATGGGCACTAATATCAGCCAGTACTAAAGAGGGACGAAAAGCGTGTTCACTAAGCTATTTTGCCTGTAAGGCCGCAGAGGCTAAACTCGGACTGGCATATATGGCTGCGAATGATa                                                                                                                                                                                <  2:172541/251‑1 (MQ=255)                        aCTAAAAAATATGGCAAGGTTATTAACAATTGTATTTTTTTCTCTATTTCTGCGAGTGCAGTTACTGCAAGACAATTTCAGAGAGAGTTAGATTTGGCAGACAAAGCAATATTATGGGCACTAATATCAGCCAGTACTAAAGAGGGACGAAAAGCGTGTTCACTAAGCTATTTTGCCTGTAAGGCCGCAGAGGCTAAACTCGGACTGGCATATATGGCTGCGAATGATAACAAGGAATTTCTcac                                                                                                                                                                <  1:98844/245‑1 (MQ=255)                        aCTAAAAAATATGGAAAGGTTATTAACACTTGTATTTTTTTCTCTAGTTCTGCGCGTGCAGTTACTGAAAGACAATTTCAGAGAGAGTTAGATTTGGCAGACAAAGCCATATTATGGACACTAATATCAGACAGTGCTAAAGAGGGACGAAAAGCGAGTTCACTAAGCTATTATGCCTGTAAGGCCGCAGAGGGTAAACTCGGACTAGCATATATGGCTGCGAATGATAACAAGGAATTtctaac                                                                                                                                                                >  2:98844/1‑242 (MQ=255)                                                                   ctATTTCTGCGAGTGCAGTTAATGCAAGACAATTTCAGAGAGAGTTAGATTTGGCAGGCAAAGCAATATTATGGGCACTAATATCAGCCAGTACTAAAGAGGGACGAAAAGCGTGTTCACTAAGCTATTTTGCCTGTAAGGCCGCAGAGGCTAAACTCGGACTGGCATATATGGCTGCGAATGATAACAAGGAATTTCTCACATCCTTATCTAATATAATGAGGTAAAAAATAGATGCTGGACTTTCCGa                                                                                                                >  2:81406/1‑250 (MQ=255)                                                                       ttCTGCGAGTGCAGTTACTGCAAGACAATTTCAGAGAGAGTTAGATTTGGCAGACAAAGCAATATTATGGGCACTAATATCAGCCAGTACTAAAGAGGGACGAAAAGCGTGTTCACTAAGCTATTTTGCCTGTAAGGCCGCAGAGGCTAAACTCGGACTGGCATATATGGCTGCGAATGATAACAAGGAATTTCTCACATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCt                                                                                                           >  1:13049/1‑251 (MQ=255)                                                                                    gTTACTGCAAGACAATTTCAGAGAGAGTTAGATTTGGCAGACAAAGCAATATTATGGGCACTAATATCAGCCAGTACTAAAGAGG‑ACGAAAAGCGTGTTCACTAAGCTATTTTGCCTTTAAGGCCGCAGAGGCTGAACTCGGACTGGCATATATGGCTGCGAATGATAACAAGGAATTTCTCCCATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCtattta                                                                                             <  1:104584/251‑1 (MQ=255)                                                                                            aaGACAATTTCAGAGAGAGTTAGATTTGGCAGACAAAGCAATATTATGGGCACTAAGATCAGCCAGTACTAAAGAGGGACGAAAAGCGTGTTCACTAAGCTATTTTGCCTGTAAGGCCGCAGAGGCTGAACTCGGACTGGCATATATGGCTGCGAATGATAACAAGGAATTTCTCACATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATtaagta                                                                                      <  2:137113/251‑1 (MQ=255)                                                                                                 aaTTTCAGAGAGAGTTAGATTTGGCAGACAAAGCAATATTATGGGCACTAATATCAGCCAGTACTAAAGAGGGACGAAAAGCGTGTTCACTAAGCTATTTTGCCTGTAAGGCCGCAGAGGCTAAACTCGGACTGGCATATATGGCTGCGAATGATAACAAGGAATTTCTCACATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGa                                                                                 <  1:48670/251‑1 (MQ=255)                                                                                                           agagTTAGATTTGGCAGACAAAGCAATATTATGGGCACTAATATCAGCCAGTACTAAAGAGGGACGAAAAGCGTGTTCACTAAGCTATTTTGCCTGTAAGGCCGCAGAGGCTAAACTCGGACTGGCATATATGGCTGCGAATGATAACAAGGAATTTCTCACATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTCTTTATTAAGTAAGGGAAAGATCATa                                                                        >  1:6171/1‑250 (MQ=255)                                                                                                            gagatAGATTTGGCAGACAAAGCAATATTATGGGCACTAATATCAGCCAGTACTAAAGAGGGACGAAAAGCGTGTTCACTAAGCTATTTTGACTGAAAGGCCGCAGAGGCTAAACTCGGAATGGCATATATGGCTGCGAATGATAACAAGGAATTTCTCACATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGCCTTTACGAATCCTATAAATGCTATTAATTAAGAAAGGGAAAAATCATaa                                                                       >  2:186291/5‑250 (MQ=255)                                                                                                                                            ggCACTAATATCAGCCAGTACTAAAGAGGGACGAAAAGCGTGTTCACTAAGCTATTTTGCCTGTAAGGCCGCAGAGGCTAAACTCGGACTGGCATATATGGCTGCGAATGATAACAAGGAATTTCTCACATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAAc                                      >  1:337583/1‑251 (MQ=255)                                                                                                                                               aCTAATATCAGCCAGTACTAAAGAGGGACGAAAAGCGTGTTCACTAAGCTATTTTGCCTGTAAGGCCGCAGAGGCTAAACTCGGACTGGCATATATGGCTGCGAATGATAACAAGGAGTTTCTCACATcc                                                                                                                                                            >  2:233654/1‑130 (MQ=255)                                                                                                                                               aCTAATATCAGCCAGTACTAAAGAGGGACGAAAAGCGTGTTCACTAAGCTATTTTGCCTGTAAGGCCGCAGAGGCTAAACTCGGACTGGCATATATGGCTGCGAATGATAACAAGGAATTTCTCACATcc                                                                                                                                                            <  1:233654/130‑1 (MQ=255)                                                                                                                                                   atatCAGCCAGTACTAAAGAGGGACGAAAAGCGTGTTCACTAAGCTATTTTGCCTGTAAGGCCGCAGAGGCTAAACTCGGACTGGCATATATGGCTGCGAATGATAACAAGGAATTTCTCACATCCTTATCTAATATAATGCGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCtatttat                                                                                            >  2:255227/1‑190 (MQ=255)                                                                                                                                                   atatCAGCCAGTACTAAAGAGGGACGAAAAGCGTGTTCACTAAGCTATTTTGCCTGTAAGGCCGCAGAGGCTAAACTCGGACTGGCATATATGGCTGCGAATGATAACAAGGAATTTCTCACATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCtatttat                                                                                            <  1:255227/190‑1 (MQ=255)                                                                                                                                                                        ggACGAAAAGCGTGTTCACTAAGCTATTTTGCCTGTAAGGCCGCAGAGGCTAAACTCGGACTGGCATATATGGCTGCGAATGATAACAAGGAATTTCTCACATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGtaaa          <  1:173626/251‑1 (MQ=255)                                                                                                                                                                         gACGAAAAGCGTGTTCACTAAGCTATTTTGCCTGTAAGGCCGCAGAGGCTAAACTCGGACTGGCATATATGGCTGCGAATGATAACAAGGAATTTCTCACATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGtaaat         >  1:316817/1‑251 (MQ=255)                                                                                                                                                                              aaaGCGTGTTCACTAAGCTATTTTGCCTGTAAGGCCGCAGAGGCTAAACTCGGACTGGCATATATGGCTGCGAATGATAACAAGGAATTTCTCACATCCTTAACTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTTAAACAGTaaataaaat    <  2:337583/251‑1 (MQ=255)                                                                                                                                                                                aGCGAGTTCAGTAAGCTATTGTGGTTGTAACACTGCAGAGGCTAAAATCGGACTGGCATATATGACTTCGAATTATAAAAAGGAATTTCTCACTTCCTTATCTAATAGAATGAGGTAAAAAATAGAAGCTGGACTTTCTGAATCCTATATATGCTACTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAATGATAAATCCACTCCAAATTGCCGCTGCTTGCATTGAAACAGtaaataaacgag  <  2:87797/251‑5 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                AGATAGCACATTAACAGTTACGGACTAAAAAATATGGCAAGGTTATTAACAATTGTATTTTTTTCTCTATTTCTGCGAGTGCAGTTACTGCAAGACAATTTCAGAGAGAGTTAGATTTGGCAGACAAAGCAATATTATGGGCACTAATATCAGCCAGTACTAAAGAGGGACGAAAAGCGTGTTCACTAAGCTATTTTGCCTGTAAGGCCGCAGAGGCTGAACTCGGACTGGCATATATGGCTGCGAATGATAACAAGGAATTTCTCACATCCTTATCTAATATAATGAGGTACAAAATAGATGCTGGACTTTCCGAATCCTATACATGCTATTTATTAAGTAAGGGAAAGATCATAAGACCATATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAA  >  NC_000913/3769229‑3769654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑