Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,402,046 G→A W3* (TGG→TGA hflK → regulator of FtsH protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,402,0460GA93.5% 85.0 / ‑3.5 32W3* (TGG→TGAhflKregulator of FtsH protease
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (14/15);  total (16/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.55e-01

GGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATGGAGGAGGACGGCAGCGTAAGTCTGCAAGTTCGTATGCCGATCGTTGACTGGCGTCGCCTCTGTAAACAAGAACCGGCGTTGATCGATTACCTGATCTAACGGCGTAGCGTCTGAAGCGTGGAGTCATATCCTCTGGCGTCGAAAGACAACAGGGATCACCGCATAACAAATATGGAGCACAAACATGGCGTGGAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTCTGAGGGAAATGGAAACAAAGGCGGTCGCGATCAAGGGCCACCTGATTTAGATGATATCTTCCGCAAACTGAGCAAAAAGCTCGGTGGTCTGGGCGGCGGTAAAGGCACCGGATCTGGCGGTGGCAGTTCATCGCAAGGCCCGCGCCCGCAGCTTGG  >  NC_000913/4401801‑4402267
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                              
ggCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATGGAGGAGGACGGCAGCGTAAGTCTGCAAGTTCGTATGCCGATCGTTGACTGGCGTCGCCTCTGTAAACAAGAACCGGCGTTGATCGATTACCTGATCTAACGGCGTAGCGTCTGAAGCGTGGAGTCATATCCTCTGGCGTCGAAAGACAACAGGGATCACCGCATAACAAATATGGAGCACAAACATGGCGTGAAATc                                                                                                                                                                                                                            >  1:145200/1‑250 (MQ=255)
        agaAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATGGAGGAGGACGGCAGCGTAAGTCTGCAAGTTCGTATGCCGATCGTTGACTGGCGTCGCCTCTGTAAACAAGAACCGGCGTTGATCGATTACCTGATCTAACGGCGTAGCGTCTGAAGCGTGGAGTCATATCCTCTGGCGTCGAAAGACAACAGGGATCACCGCATAACAAATATGGAGCACAAACATGGCGTGAAAGCAGCCCGGta                                                                                                                                                                                                                   <  1:83838/251‑1 (MQ=255)
         gaAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATGGAGGAGGACGGCAGCGTAAGTCTGCAAGTTCGTATGCCGATCGTTGACTGGCGTCGCCTCTGTAAACAAGAACCGGCGTTGATCGATTACCTGATCTAACGGCGAAGCGTCTGAAGCGTGGAGTCATATCCTCTGGCGCCGAAAGACAACAGGGAACACCGCATAACAAATATGGAGCACAAACATGGCGTGGAAACAACCCggca                                                                                                                                                                                                                   >  2:126606/1‑248 (MQ=255)
                     atcagtgtCAGGCATTATAAAAAGAGTGGATGGAGGAGGCCCTCAGATTAAATCTGCAAGTGCGTGGGCCGATCGTTGATTGGCGTCGCCTCTTTACACAAGAACCTGCGTTGATCGACTACCTGTACTAACTGCGTAGCGTCTGAAGCGTGGAGTCATATCCTCTGTCGTCGAAAGACAACAGTGATCACAGCATCACAAATGTGGAGTACAACCATGGCGTTAACTCAGCCCgttcatagctgacacga                                                                                                                                                                                                      <  2:288286/244‑17 (MQ=255)
                           ttCAGGCAATAGAAAAAGAGTGGATGGAGGAGGACGGCAGCGTAAGTCTGCAAGTTCGTATGCCGATCGTTGACTGGCGTCGCCTCTGTAAACAAGAACCGGCGTTGATCGATTACCTGATCTAACGGCGTAGCGTCTGAAGCGTGGAGTCATATCCTCTGGCGTCGAAAGACAACAGGGATCACCGCATAACAAATATGGAGCACAAACATGGCGTGAAATCAGCCCGGTAATAACGGACAAGACcgcg                                                                                                                                                                                                 >  1:29922/1‑250 (MQ=255)
                                  actaaaaaacaagTGTATGGAGGAGGACGGCAGCGTAAGTCTGCAAGATCGTATGCCGATCGGTGACTGGCGGCGCCTCTGTAAAAAAGAACCGGCGTTGATCGATTACCTGATCTAACGGCGTATCGTCTGAAGCGTGGAGTACTATCCTCTGGCGGCGAAAGACAACAGGGATCACCGCATAACAAATATGGCGCAAAAACATGGAGGGAAAACAGCCCGGTACTAACAGGCAAGaacgaaacacgtgg                                                                                                                                                                                         >  2:185193/12‑238 (MQ=255)
                                        aaaaGAGTGGATGGAGGAGGACGGCAGCGTAAGTCTGCAAGTTCGTATGCCGATCGTTGACTGGCGTCGCCTCTGTAAACAAGAACCGGCGTTGATCGATTACCTGATCTAACGGCGTAGCGTCTGAAGCGTGGAGTCATATCCTCTGGCGTCGAAAGACAACAGGGATCACCGCATAACAAATATGGAGCACAAACATGGCGTGAAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAagc                                                                                                                                                                                   >  1:233054/1‑251 (MQ=255)
                                           agagTGGATGGAGGAGGACGGTAGCGTAATTCTTCAAGTTCGTAGGCTGTTCGTTGTCTGGCGTCGCCTCTGTAAACAAGAACCGGCGTTGATCGATTTCCTGATCTAACGGCGTAGCGTCTGAAGCGTGGAGTCATATCCTCTGGCGTCGAAAGACAACAGGGATCACCGCATAACAAATATGGAGCACAAACATGGCGTGAAATCAGCCCGGTAATAACGGACAAGACCGAGACCCGTGGGGAagcagc                                                                                                                                                                                <  2:204820/251‑1 (MQ=255)
                                                  atgcaggaggaCTTCAGCGTAAGTCTGAAAGTTCGTATGCCGATCGTTGACATGCGTCGCCTCTGTAAACAAGAACCGGCGTTGATCGATTACCTGATCTAACGGCGAAGCGTCTGAAGCGTGGAGTCATATCCTCTGGCGTCGAAAGACAACAGGGATCACCGCATAACAAATACGGCGGACAAACATGGAGTGAAATCAGCGCGGTAATAACGAACAAGACCGAGACCCGTGGGGATGCAGCAAACCt                                                                                                                                                                          >  2:10448/5‑250 (MQ=255)
                                                       ggaggaCGGCAGCGTAAGTCTGCAAGTTCGTATGCCGATCGTTGACTGGCGTCGCCTCTGTAAACAAGAACCGGCGTTGATCGATTACCTGATCTAACGGCGTAGCGTCTGAAGCGTGGAGTCATATCCTCTGGCGTCGAAAGACAACAGGGATCACCGCATAACAAATATGGAGCACAAACATGGCGTGAAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTggcggc                                                                                                                                                                    <  2:306291/251‑1 (MQ=255)
                                                                        gTCTGCAAGTTCGTATGCCGATCGTTGACTGGCGTCGCCTCGGTAAACAAGAACCGGCGTGGATCGATTACCTGATCTAACGGCGTCGCGTCTGAAGCGTGGAGTCATATCCTCTGGCGTCGAAAGACAACAGGGATCACCGCATAACAAATATGGAGCACAAACATGGCGTGAAATCATCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTCTGAGGGAAATgg                                                                                                                                                   <  2:282610/251‑1 (MQ=255)
                                                                                  acGTATGCCGATCGGTGACTGGGGTCGGCTCGTTAAACAAGAACCGGCGTTGATCTATTACCTGATCTAACGGAGTAGCGTCTGAAGCGTGGAGTCATATCCTCGGGCGTCGAAAGACAACAGGGATCACCGCATAACAAATATGGAGCACAAACATGGCGGGAAATCAGCACGGTAATAACGGACAAGAGCGCGACCCGGGGGGAAGCAGCAAACCTGGCGCTAACTCTGAGGGAAATGGAAACAAAgg                                                                                                                                          <  2:50437/249‑1 (MQ=255)
                                                                                        gCCGATCGTGGACTGGCGTCGCCTCTGTAAACAAGAACCGGCGTTTATCGATTACCTGATCTAACGGCGTAGCGTCTGAAGCGTGGAGTCATATCCTCTGTCGTCGAAAGACAACAGGGATCACCGCATAACAAATATGGAGCACAAACATGGCGTGAAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTCTGAGGGAAATGGAAACAAAGGCGGTcg                                                                                                                                    <  1:309235/250‑1 (MQ=255)
                                                                                           gATCGTTGACTGGCGTCGTCGCTGTAAACAAGAACCGGCGTTGATCGCTTACCTGATCTAACGGCGGAGCGTCTGAAGCGTGGAGTCATATCCGCGGGCGTCGAAAGACAACAGGGATCACCGCATAACAAATATGGAGCACAAACATGGCGTGAAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTCTGAGGGAAATGGAAACAAAGGCGGCCGCGAt                                                                                                                                <  2:131079/251‑1 (MQ=255)
                                                                                                           cGCCTCTGTAAACAAGAACCGGCGTTGATCGCTTACCTGATCTAACGGCGTAGAGTCTGAAGCGTGGAGTCATATCCTCTGGCGTCAAAAGACAACAAGAATCACCGCATACCAAATATGGAGCACAAACATGGAGTGAAATCAGCCCGGTAATCACGGACCAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTCTGAGGGAAATGGAAACAAAGGCGGTCGCGATCAAGAGCCACATGAtt                                                                                                                >  1:316502/1‑251 (MQ=255)
                                                                                                                 tGTAAACAAGAACCGGCGTTGATCGATTACCTGATCTAACGGCGTAGCGTCTGAAGCGTGGAGTCATATCCTCTGGCGTCGAAAGACAACAGGGATCACCGCATAACAAATATGGAGCACAAACATGGCGTGAAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTCTGAGGGAAATGGAAACAAAGGCGGTCGCGATCAAGGGCCACCTGATTTAgatg                                                                                                         >  1:201189/1‑251 (MQ=255)
                                                                                                                  gTAAACAAGAACCGGCGTTGAACGATTTCCTGATCGAACGGCGTATCGGCTGAAGCGTGGAGTCATATGCGCTGGCGTCGAAAGACAACAGGGATCACCGCATAACAAATATGGAGCACAAACATGGCGTGAAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTCTGAGGGAAATGGAAACAAAGGCGGTCGCGATCAAGGGCCACCTGATTTAga                                                                                                            <  2:326012/248‑1 (MQ=255)
                                                                                                                               ggCGTTGATCGATTACCTGATCTAACGGCGTAGCGTCTGAAGCGTGGAGTCATATCCTCTGGCGTCGAAAGACAACAGGGATCACCGCATAACAAATATGGAGCACAAACATGGCGTGGAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTCTGAGGGAAATGGAAACAAAGGCGGt                                                                                                                                      <  1:50223/209‑1 (MQ=255)
                                                                                                                                 cGTTGATCGATTACCTGATCTAACGGCGTAGCGTCTGAAGCGTGGAGCCATATCCTCTGGCGACGAAAGACAACAGGGATCACAGAATAACAAATATGGAGCACAAACATGGCGTGAAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGACGCAGAAAACCTGGAGGCACCTCTGAGGGAAATGGAAACAAAGGCGGTCACGATCAAGGGCCACCTGATTTGAGTGATCTCTTCCCCAAACt                                                                                         >  2:224959/1‑251 (MQ=255)
                                                                                                                                  gttgatcgcgctccgtaccTAACGGCGTAGCGTCTGAAGCGTGGAGTCATATCCTCGGGCGTCGAAAGACAACAGGGAGCACCGCATAACAAATATGGAGCACAAACATGGCGTGAAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTCTGAGGGAAATGGAAACAAAGGCGGACGCGATCAAGGGCCACCTGATTTAGATGATATCTTCCGCAAAcgg                                                                                        <  2:95558/233‑3 (MQ=255)
                                                                                                                                     gaacGATTTCCTGATCTAACGGCGTTGCGTCTGAAGCGTGGAGTCATATCCGCTGGCGTCGAAAGTCAACAGGGATCACCGCAGAACAAATATGGAGCACAAACATGGCGGGAAATCAGCCCGGGAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCCGGCGGCACCTTTGAGGGAAATTGAAACAAAGGCGGTCGCTATCCAGGGCCACCTGATTTAGATGATATCTTCAGCaatcctagc                                                                                     <  2:125473/248‑8 (MQ=255)
                                                                                                                                                                                            gCGTCGAAAGACAACAGGGATCACCGCATAACAAATATGGAGCACAAACATGGCGTGAAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTCTGAGGGAAATGGAAACAAAGGCGGTCGCGATCAAGGGCCACCTGATTTAGATGATATCTTCCGCAAACTGAGCAAAAAGCTCGGTGGTCTGGGCGGCTGTAAAGGCACCGGATCTGGCGGTGGCAGtt                              <  1:99591/251‑1 (MQ=255)
                                                                                                                                                                                                      acaacaGGGATCACCGCCTAACCAATATGGAGCACAAACATGGCGTGAAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCCGCAAACCTGGCGGCAACTCTGAGGGAAATGGAAACAAAGGAGGTCGCGATCAAGGGCCa                                                                                                                       >  2:49986/1‑153 (MQ=255)
                                                                                                                                                                                                      acaacaGGGATCACCGCATAACAAATATGGAGCACAAACATGGCGTGAAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTCTGAGGGAAATGGAAACAAAGGCGGTCGCGATCAAGGGCCa                                                                                                                       <  1:49986/153‑1 (MQ=255)
                                                                                                                                                                                                         acaGGGATCACCGCATAACAAATATGGAGCACAAACATGGCGTGAAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTCTGAGGGAAATGGAAACAAAGGCGGTCGCGATCAAGGGCCACCTGATTTAGATGATATCTTCCGCAAACTGAGCAAAAAGCTCGGTGGTCTGGGCGGCGGTAAAGGCACCGGATCTGGCGGTGGCAGTTCATCGCAAGgccc                 <  1:126606/251‑1 (MQ=255)
                                                                                                                                                                                                          caGGGATCACCGCATAACAAATATGGAGCACAAACATGGCGTGAAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTCTGAGGGAAATGGAAACAAAGGCGGTCGCGATCAAGGGCCACCTGATTTAGATGATATCTTc                                                                                                 >  2:161788/1‑170 (MQ=255)
                                                                                                                                                                                                          caGGGATCACCGCATAACAAATATGGAGCACAAACATGGCGTGAAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTCTGAGGGAAATGGAAACAAAGGCGGTCGCGATCAAGGGCCACCTGATTTAGATGATATCTTc                                                                                                 <  1:161788/170‑1 (MQ=255)
                                                                                                                                                                                                          caGGGATCACCGCATAACAAATATGGAGCACAAACATGGCGTGAAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTCTGAGGGAAATGGAAACAAAGGCGGTCGCGATCAAGGGCCACCTGATTTAGATGATATCTTCCGCAAACTGAGCAAAAAGCTCGGTGGTCTGGGCGGCGGTAAAGGCCCCGGATCTGGCGGTGGCCGGTCATCGCCAGgcccg                >  1:230815/1‑251 (MQ=255)
                                                                                                                                                                                                                 cACCGCATAACAAATATGGAGCACAAACATGGCGTGAAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTCTGAGGGAAATGGAAACAAAGGCGGTCGCGATCAAGGGCCACCTGATTTAGATGATATCTTCCGCAAACTGAGCAAAAAGCTCGGTGGTCTGGGCGGCGGTAAAGGCACCGGATCTGGCGGTGGCAGGTCATCGCAAGgcccgcgcccgc         >  1:59076/1‑251 (MQ=255)
                                                                                                                                                                                                                  aCCGCATAACAAATATGGAGCACAAACATGGCGTGAAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTCTGAGGGAAATGGAAACAAAGGCGGTCGCGATCAAGGGCCACCTGATTTAGATGATATCTTCCGCAAACTGAGCAAAAAGCTCGGTGGTCTGGGCGGCGGTAAAGGCACCGGATCTGGCGGTGGCAGTTCATCGCAAGgcccgcccccgcc        >  1:250330/1‑250 (MQ=255)
                                                                                                                                                                                                                       aTAACAAATATGGAGCACAAACATGGCGTGAAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTCTGAGGGAAATGGAAACAAAGGCGGTCGCGATCAAGGGCCACCTGATTTAGATGATATCTTCCGCAAACTGAGCAAAAAGCTCGGTGGTCTGGGCGGCGGTAAAGGCACCGGATCTGGCGGTGGCAGTTCATCGGAAGGGCCGCGCCCGCAGcctg   >  1:121970/1‑248 (MQ=255)
                                                                                                                                                                                                                        tAACAACTATGGAGCACAAACATGGCGTGCAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTATGAGGGAAATGGAAACAAAGGCGGTCGCGATCAAGGGCCACCTGATTTAGATGATATCTTCCGCAAACTGAGCAAAAAGCTCGGTGGTCTGGGCGGCGGTAAAGGCACCGGATCTGGCGGTGGCAGGTCCTCGCGAGCGCCGCGGCCGCaaggtgg  >  2:129598/1‑245 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                              
GGCGTCTGAGAAGTCGTTTTTATCAGCTTCAGGCAATAGAAAAAGAGTGGATGGAGGAGGACGGCAGCGTAAGTCTGCAAGTTCGTATGCCGATCGTTGACTGGCGTCGCCTCTGTAAACAAGAACCGGCGTTGATCGATTACCTGATCTAACGGCGTAGCGTCTGAAGCGTGGAGTCATATCCTCTGGCGTCGAAAGACAACAGGGATCACCGCATAACAAATATGGAGCACAAACATGGCGTGGAATCAGCCCGGTAATAACGGACAAGACCGCGACCCGTGGGGAAGCAGCAAACCTGGCGGCAACTCTGAGGGAAATGGAAACAAAGGCGGTCGCGATCAAGGGCCACCTGATTTAGATGATATCTTCCGCAAACTGAGCAAAAAGCTCGGTGGTCTGGGCGGCGGTAAAGGCACCGGATCTGGCGGTGGCAGTTCATCGCAAGGCCCGCGCCCGCAGCTTGG  >  NC_000913/4401801‑4402267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: