Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,452,218	G→A	F23F (TTC→TTT)	rplD ←	50S ribosomal subunit protein L4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,452,218	0	G	A	100.0%	162.0 / NA	49	F23F (TTC→TTT)	rplD	50S ribosomal subunit protein L4
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/22);  total (27/22)

CGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTGAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAAC  >  NC_000913/3451977‑3452462                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     cgacgcggtacactgtcctGTTAACTTGTTGACTGTGGTCCTGCTAACTAGAAGCAAAGGGCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGATCCCGGCCGGCGCCTTTCCGGCGCCCTGGTTTTTTACCGGAACCAGTTTCTTCAGCACGAGTCTTCTGAGTACGAGTTCCCTGACGAGCTCCAACTGCATAAGCAACAACAACCTGGTGACCCTGCGCTTCGTTAAAATCa                                                                                                                                                                                                                                                <  2:50462/230‑1 (MQ=255)             tcttctTGTTAACTTTTTGACTGTGGTCCTGCGAACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGtt                                                                                                                                                                                                                                 >  1:118735/1‑251 (MQ=255)             gcttcttGTTAACTGTTTGACTGGCGTCCTGCGAACTAGCAGCAAAGGTCACGCTTCCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGTCTTTCTGTCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTTCCCGGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGt                                                                                                                                                                                                                                  <  1:187444/249‑1 (MQ=255)                           ttttGACTGTGGTCCTGCGAACGAGCAGCAAAGGTCACGCCACCCGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGAACGGCCGGTGCCTTTATGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCACCAACAACCTGGCGAACCAGCGCTTCGTTAAAATCACGAACGGAGGGAGTTTCGGAAACAGTCa                                                                                                                                                                                                                    >  2:319055/1‑250 (MQ=255)                              tGACTGTGGGCCTGCGAACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAgcg                                                                                                                                                                                                                 <  2:79353/250‑1 (MQ=255)                                aCTGTGGTCCTGCGAACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGCGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAgcgcgc                                                                                                                                                                                                              >  1:244504/1‑251 (MQ=255)                                   gtgGTCCTGCGAACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGctct                                                                                                                                                                                                           >  1:91915/1‑251 (MQ=255)                                        ccTGCGAACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGcgcg                                                                                                                                                                                                      >  1:65902/1‑251 (MQ=255)                                         cTGCGAACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGt                                                                                                                                                                                                     >  1:55910/1‑251 (MQ=255)                                           gcgaaCGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGt                                                                                                                                                                                                     <  1:31252/245‑1 (MQ=255)                                                     agcaAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACt                                                                                                                                                                                          >  1:335822/1‑250 (MQ=255)                                                           ggTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTcc                                                                                                                                                                                    >  1:118537/1‑250 (MQ=255)                                                                 gCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGCGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCt                                                                                                                                                                              >  1:308044/1‑250 (MQ=255)                                                                   caccaGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCCCTGCGCGTCTTTCAATACTAATTCCATTGATAtc                                                                                                                                                                           >  2:376/1‑251 (MQ=255)                                                                      caGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTcc                                                                                                                                                                        >  1:50796/1‑251 (MQ=255)                                                                       aGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCt                                                                                                                                                                       >  1:181434/1‑251 (MQ=255)                                                                           cGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTAcc                                                                                                                                                                   >  1:263932/1‑250 (MQ=255)                                                                                   cGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGTTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTcaca                                                                                                                                                           <  2:118735/251‑1 (MQ=255)                                                                                           tGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCAGAAGCAACAACAACCTGGTGAACCAGGTCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTGTCAATACTAATTCCATTGCTATCTCCTTACGCCTGAACAGCTgggt                                                                                                                                                    <  2:50796/250‑3 (MQ=255)                                                                                            gATAGAACCAGAATGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACGTCAGCACGAGTCTTCTGAGCACGAGGACCCTGACGAGCACCATCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAATGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTa                                                                                                                                                  <  1:333478/251‑1 (MQ=255)                                                                                                  aCCAGAACGCGACCGGCCGGTGCTGTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACCTCACCACGATGCTTATGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATc                                                                                                                                            <  2:181434/251‑1 (MQ=255)                                                                                                           cgcACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTa                                                                                                                                   <  1:158607/251‑1 (MQ=255)                                                                                                                 gCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGtt                                                                                                                             >  1:223132/1‑251 (MQ=255)                                                                                                                       gCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCAcc                                                                                                                        <  1:220457/250‑1 (MQ=255)                                                                                                                         cTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCgg                                                                                                                     >  1:144484/1‑251 (MQ=255)                                                                                                                                             ttACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGAACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCAGGTGAGCCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCGCCTTCCGCCTTCACACCTGGTTCAACGATCAAGTCGCTACCGGTTGCACCAGGGACAGCACCTTTAACcagc                                                                                                  >  2:272028/1‑250 (MQ=255)                                                                                                                                                 cGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACcagcagcag                                                                                             <  1:319055/251‑1 (MQ=255)                                                                                                                                                  ggAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCCCAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAgg                                                                                            >  1:325857/1‑251 (MQ=255)                                                                                                                                                                      gAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAAcgcg                                                                        >  2:120111/1‑251 (MQ=255)                                                                                                                                                                          cgtcagAGCACGAGTACCCTGACGGGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCTCTTCGTTAAAAGCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGtac                                                                     <  2:244504/245‑1 (MQ=255)                                                                                                                                                                                  cACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAg                                                            <  1:111905/251‑1 (MQ=255)                                                                                                                                                                                    cgcgtaaacTGACGAGCACCAGAGGCATAAGCAACAACAACCTGGTGAACCCGCGCGTCGTTAAAAGCACGACCGTAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTTGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAATGCGTACTACGTCAAGGc                                                          <  2:254034/243‑1 (MQ=255)                                                                                                                                                                                    cGAGTACCCTGTCGAGCACCAGCTGCATAAGCTACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGAACCCGGGAAAGCACCTTTAACCAGCAGCATGTTGAGCTCAGCGTCAACGCGTACTGCGTccagac                                                          >  2:131019/1‑246 (MQ=255)                                                                                                                                                                                     gAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACCGCACCTTTAACAAGCAGCAGGTTGCGATCAGCGTCAACGCGTACTACGTCAAGGct                                                         >  2:188780/1‑251 (MQ=255)                                                                                                                                                                                          cccTGACGAGCACCAGCTGAATAAGCAACAACAACCTGGTGAACCAGTGCTTCGGTAAAATCACGACCGAAGGTAGTTTCTGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGGTTAACGATCAGGGCGCTACCTGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGTTTGCGCTCAGCGTCAACGCGTTCTTCGTCAATGCTCTGaa                                                    <  2:288899/251‑1 (MQ=255)                                                                                                                                                                                             tGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGt                                                                       >  2:290922/1‑229 (MQ=255)                                                                                                                                                                                             tGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGt                                                                       <  1:290922/229‑1 (MQ=255)                                                                                                                                                                                                   gCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAACTCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCt                                                                                                                                                                >  2:270298/1‑134 (MQ=255)                                                                                                                                                                                                   gCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGGCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCt                                                                                                                                                                <  1:270298/134‑1 (MQ=255)                                                                                                                                                                                                       catcttcttAAGCAACATCTACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTTGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCAGTTCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGTACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACAcgttc                                       <  2:223132/243‑1 (MQ=255)                                                                                                                                                                                                       cAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACAcgttc                                       <  1:36829/251‑1 (MQ=255)                                                                                                                                                                                                              aTAAGAAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGATTCGGAAACAGTCAGCGCGCTCTGCACGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGCATGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTATGAACGGTTACACGTTCGTTcccc                                >  1:234243/1‑251 (MQ=255)                                                                                                                                                                                                                aaGCAACAACAACCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCAt                              >  1:44538/1‑251 (MQ=255)                                                                                                                                                                                                                         caacCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGc                     >  1:94463/1‑251 (MQ=255)                                                                                                                                                                                                                          aacCTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGcc                    <  1:188780/251‑1 (MQ=255)                                                                                                                                                                                                                             cTGGTGAACCAGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCACCGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCAtt                 >  1:234765/1‑251 (MQ=255)                                                                                                                                                                                                                                       aGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCtt       <  1:376/251‑1 (MQ=255)                                                                                                                                                                                                                                       aGCGCTTCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCtt       <  1:302096/251‑1 (MQ=255)                                                                                                                                                                                                                                            ttCGTTAAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAac  >  1:67095/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     CGCCGCGGTACATCTTCTTGTTAACTTTTTGACTGTGGTCCTGCGGACGAGCAGCAAAGGTCACGCCACCAGAACGCCAGATCGGGCTCTTGATAGAACCAGAACGCGCACGGCCGGTGCCTTTCTGGCGCCACGGTTTTTTACCGGAACCAGTTACTTCAGCACGAGTCTTCTGAGCACGAGTACCCTGACGAGCACCAGCTGCATAAGCAACAACAACCTGGTGAACCAGCGCTTCGTTGAAATCACGACCGAAGGTAGTTTCGGAAACAGTCAGCGCGCTCTGCGCGTCTTTCAATACTAATTCCATTGCTATCTCCTTACGCCTTCACAGCTGGTTTAACGATCAGGTCGCTACCGGTTGCACCCGGGACAGCACCTTTAACCAGCAGCAGGTTGCGCTCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAAC  >  NC_000913/3451977‑3452462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑