Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,484,827	C→T	L152F (CTT→TTT)	evgS →	sensor histidine kinase EvgS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,484,827	0	C	T	100.0%	34.6 / NA	12	L152F (CTT→TTT)	evgS	sensor histidine kinase EvgS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/7);  total (5/7)

CGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAA  >  NC_000913/2484597‑2485059                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         cGCAGAAACGGGGTCGTGGTATTAATGCTGATTAGTTAAAGCTGTTAAAAAGAGAGTTAAATATCAAAGTAACACTCCGGGAATACGAAGATCAGCAAAAAGCAAAGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACAGTTAGTTAATTCGCCGCCTCGTAATAATGACATTGCTGCAACCAAACCATTGATAATTGTCATTCCGGCGCTGGTAACCACCTGTCACGACGAAATGCGAca                                                                                                                                                                                                                       <  2:75841/250‑2 (MQ=255)                     aTTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAAGTAACACTCCGGGAATACTCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCTGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAATCCAGt                                                                                                                                                                                                  <  1:212230/251‑1 (MQ=255)                            atGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTACGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAataat                                                                                                                                                                                           <  2:10256/250‑3 (MQ=255)                                                           aaaTATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAgg                                                                                                                                                            >  1:106457/1‑251 (MQ=255)                                                                       aaCACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCatcaat                                                                                                                                                <  1:110023/251‑1 (MQ=255)                                                                                                                      cAGAAGGTGAAGTCGATATAGCGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAACGCAACAATTATCTCTTTTACAAATTTATATCAGGc                                                                                                  >  2:95096/1‑250 (MQ=255)                                                                                                                                            gtTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTggg                                                                            <  1:66438/250‑1 (MQ=255)                                                                                                                                                 cacaTTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGAAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAAt                                                                      >  2:283575/1‑251 (MQ=255)                                                                                                                                                  acaTTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACAATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGAGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTATCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATg                                                                     >  2:30265/1‑251 (MQ=255)                                                                                                                                                                                                            aTTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTtcactca           <  1:67662/251‑1 (MQ=255)                                                                                                                                                                                                                ccTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTcc       >  1:164123/1‑251 (MQ=255)                                                                                                                                                                                                                     cctgcgcTGGTAAACACCTCTCACGACTCAATGCGAACTCTTACCTAACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCTCAGACGAGGTAATTCATCAAACATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCTGTCTCAGCTGGGCACAATGATTTCTTTATTGTTAGTAACATCATTACCAGCAGTATGATTGTCCGCTATTTCACTCACTCCTTaaa  <  2:236955/248‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         CGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAA  >  NC_000913/2484597‑2485059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑