Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,452,714	G→A	Q48Q (CAG→CAA)	ytfT →	galactofuranose ABC transporter putative membrane subunit YtfT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,452,714	0	G	A	100.0%	70.6 / NA	25	Q48Q (CAG→CAA)	ytfT	galactofuranose ABC transporter putative membrane subunit YtfT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/14);  total (11/14)

ATTATCATGCGCGATCGCAAACAGGTGGCGGAGATCCCGCTGGCAGAGCTTTCCGTTCCGGCGATCATGAACGCCATTGCGGCGTAAGGAGAACAGTGTGATGCCTCAATCTCTTCCGGACACCACTACGCCAAAAAGGCGCTTTCGCTGGCCGACGGGGATGCCGCAGCTGGTAGCACTATTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATC‑GCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCGGGATTCAGCCTGCCGATTGTTTTGTTAAGCGCCCTGG  >  NC_000913/4452474‑4452916                                                                                                                                                                                                                                                 |                                                                                                                                                                                                            aTTATCATGCGCGATCGCAAACAGGTGGCGGAGATCCCGCTGGCAGAGCTTTCCGTTCCGGCGATCATGAACGCCATTGCGGCGTAAGGAGAACAGTGTGATGCCTCAATCTCTTCCGGACACCACTACGCAAAAAAGGCGCTTTCGCTGGCCGACGGGGATGCCGCAGCTGGTAGCACTATTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAAGATGGGCGtt                                                                                                                                                                                                   <  1:335518/251‑1 (MQ=255)   tATCATGCGCGATCGCAAACAGGTGGCGGAGATCCCGCTGGCAGAGCTTTCCGTTCCGGCGATCATGAACGCCATTGCGGCGTAAGGAGAACAGTGTGATGCCTCAATCTCTTCCGGACACCACTACGCCAAAAAGGCGCTTTCGCTGGCCGACGGGGATGCCGCAGCTGGTAGCACTATTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAAGATGGGCGTTTg                                                                                                                                                                                                 >  1:103416/1‑251 (MQ=255)        tGCGCGATCGCAAACAGGTGGCGGAGATCCCGCTGGCAGAGCTTTCCGTTCCGGCGATCATGAACGCCATTGCGGCGTAAGGAGAACAGTGTTATGCCTCAATCTCTTCCGGACACCACTACGCCAAAAAGGCGCTTTCGCTGGCCGACGGGGATGCCGCAGCTGGTAGCACTATTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAAGATGGGCGTTTGTTCgg                                                                                                                                                                                            >  1:326867/1‑251 (MQ=255)                              ggAGATCCCGCTGGCAGAGCTTTCCGTTCCGGCGATCATGAACGCCATTGCGGCGTAAGGAGAACAGTGTGATGCCTCAATCTCTTCCGGACACCACTACGCCAAAAAGGCGCTTTCGCTGGCCGACGGGGATGCCGCAGCTGGTAGCACTATTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAAGATGGGCGTTTGTTCGGt                                                                                                                                                                                           <  1:18620/230‑1 (MQ=255)                              ggAGATCACGCTGGCAGAGCTTTCCGTTCCGGCGATCATGAACGCCATTGCGGCGTAAGGAGAACAGTGTGATGCCTCAATCTCTTCCGGACACCACTACGCCAAGAAGGCGCTTTCGCTGGCCGACGGGGATGCCGCAGCTGGTAGCACTATTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGGGGTGCTTCAAGCTGGGCGTTTGTTCggc                                                                                                                                                                                           >  2:18620/1‑229 (MQ=255)                                       gCTGGCAGAGCTTTCCGTTCCGGCGATCATGATCGCCATTGCGGCGTAAGGAGAACAGTGTGATGCCTCAATCTCTTCCGGACACCACTACGCCAAAAAGGCGCTTTCGCTGGCCGACGGGGATGCCGCAGCTGGTAGCACTATTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAAGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCt                                                                                                                                                             >  1:279051/1‑251 (MQ=255)                                          ggCAGAGCTTTCCGTGTCGGCGATCATGGACGGCATTGCGTCGTAAGGAGAACAGGGTGATGCCTCAATCTCTGCCGGACACCACTACGCCAAAAAGGCGCTTGCGCTGGCCTACGGGGATGCCGCAGCTGGTAGCACTATTGCTGGTGCTGCTGGTCGCTAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAAGATGGGCTCTTGGTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTccc                                                                                                                                                          <  2:122838/251‑1 (MQ=255)                                            cAGAGCTTTCCGTTCCGGCGATCATGAACGCCATTGCGGCGTAAGGAGAACAGTGTGATGCCTCAATCTCTTCCGGCCACCACTACGCCAAAAAGGCGCTTTCGCTGGCCGACGGGGATGCCGCAGCTGGTAGCACTATTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAAGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGt                                                                                                                                                        >  1:157694/1‑251 (MQ=255)                                                   gtccgttacgGCGATCATGAACTCCCTTGCGGCGTAAGGAGTACAGTGTGATGCCTCAATCTCTTCCGGACACCTCTACGCCAAACAGGCGCTGTCGCTGGCCGACGGGGATTCCTCCGCTGGTAGCACTCTTGCTGGGGCTGCTGGTCGATAGCCTTGTCGCCCCGCATTTCTGGCAGGTGGTGCTTAAAGATGGGCGTCTGTTCGGGAGCACCATAGACATTCTTAACCTTGCAGCGCCCGTTGCGtt                                                                                                                                                  <  2:278747/249‑1 (MQ=255)                                                                                    ggaaggagaccaggggtaggCCTCAATCTCTTCCGGACACCTCGATGCCTAAAAGGCTGTTGCGTTGGCCGACGGGGATGCCGCAGCTGGTAGCCTTATTGCTTGTGCTGCGGGTCGATAGCCTGGTAGCCTCGCATTTCTGGCAGGTGGTGCTACAAGATGGGCGTTTGTTCGGTAGCCCCAGAGTCATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTTGTGATC‑GCCAcc                                                                                                               <  2:15316/232‑1 (MQ=255)                                                                                     tAAGGAGAACAGTGTGATGCCTCAATCTCTTCCGGACACCACTACGCCAAAAAGGCGCTTTCGCTGGCCGACGGGGATGCCGCAGCTGGTAGCACTATTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAAGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATC‑GCCACCg                                                                                                              >  1:143370/1‑251 (MQ=255)                                                                                           gaACAGTGTGATGCCTCAATCTCTTCCGGACACCACTACGCCAAAAAGGCGCTTTCGCTGGCCGACGGGGATGCCGCAGCTGGTAGCACTATTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAAGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATC‑GCCACCGGTggg                                                                                                         >  1:333176/1‑250 (MQ=255)                                                                                             aCAGTGTGATGCCTCAATCTCTTCCGGACACCACTACGCCAAAAAGGCGCTTTCGCTGGCCGACGGGGATGCCGCAGCTGGTAGCACTATTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAAGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATC‑GCCACCGGTGGGAtt                                                                                                      <  1:36413/251‑1 (MQ=255)                                                                                                 tgtgATGCCTCAATCTCTTCCGGACACCACTACGCCAAAAAGGCGCTTTCGCTGGCCGACGGGGATGCCGCAGCTGGTAGCACTATTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAAGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATC‑GCCACCGGTGGGATTGAtc                                                                                                  >  1:262851/1‑251 (MQ=255)                                                                                                       gccgcaacctctGCCGGACACCACTACGCCAAAAAGGCGCTGTCGCTGGCCGACGGGGATGCCGCAGCTGGTAGCACTCTTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAAGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATC‑GCCACCGGTGGGATTGATCTCTcc                                                                                             <  2:284180/242‑1 (MQ=255)                                                                                                           cAATCTCTTCCGGACACCCCTACGCCAAAAAGGCGCTTTCGCTGTCCAACGGGGATGCCGCAGCTGGCAGCACTATTGCTGGTGCTGCTGGTCGATAGCCTGCTGGCCCCGCATTTCTGCCAGGGGGTGCTTCAAGATAGGCGTTGGTTCGGTAGCCCCATAGACATTCTTACCCGTGCAGCTCCAGTTGCGTGACTGGCGCTTGGCATTAAGCTGGGGATCCGCC‑CCGGTGGGATTGATCTCTCCGTAgg                                                                                        >  2:259280/1‑251 (MQ=255)                                                                                                                         caccacTACGCAAAAAAGGCGCTATAGCTGGCCGCCGGGGATGCCGCAGCTGGTAGCACTATTGCTGGTGCTGCTGGTCGATAGCCTGGCAGCCCCGCATTTCTGGCAGGTGGAGCTTCCAGATGGGCGTTTGTTCGGTAGCCCCATAGCCTTTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGCGATC‑GCCCCCGGTGGGATTGACCTCTCCGTTGGGGCggggggtgcgt                                                                          >  2:316717/1‑242 (MQ=255)                                                                                                                                                      ggCCGACGGGGATGCCGCAGCTGGTAGCACTATTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAAGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATGGGCATGACGCTGGTGAGC‑GCCTCCGGTGGGATTTATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACg                                             <  1:335044/251‑1 (MQ=255)                                                                                                                                                                   gccgcagagtgtagctcgtgtGCTGGTGGTGCTGGTTGATAGCCTGGTAGCCCCGAATTTCTGGCAGGTGTTGCTTCAAGATGGGCGTTTGTTCGGTAGCCACATAGACATTCTTACCCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATC‑GCCACCGGTGGGATTGATCTCTCCGGAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCGGGAtgca                                <  2:279051/231‑4 (MQ=255)                                                                                                                                                                       cagcggggaGCACTATGGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTGCAAGATGGGCGTTTGTTCGGGAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGAGC‑GCCACCGGTGGGAGTGAGCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCGGGATTCAGCCt                            <  1:316717/243‑1 (MQ=255)                                                                                                                                                                        aGCTGGTAGCACTATTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAAGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATC‑GCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCGGGATTCAGCCTg                           <  2:103416/251‑1 (MQ=255)                                                                                                                                                                                   cTATTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAAGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATC‑GCCACCGGTGGGATTGATCTCTCCGTAgg                                                                                        <  2:155611/179‑1 (MQ=255)                                                                                                                                                                                   cTATTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAAGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATC‑GCCACCGGTGGGATTGATCTCTCCGTAgg                                                                                        >  1:155611/1‑179 (MQ=255)                                                                                                                                                                                                 gctgGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAAGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATC‑GCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGTAGCCACAACGGCTGCGATGACGGTCGCGGGATTCAGCCTGCCGATTGTTTTGTTAAGCGCCCTgg  <  1:210325/251‑1 (MQ=255)                                                                                                                                                                                                 gctgGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAAGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATC‑GCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCGGGATTCAGCCTGCCGATTGTTTTGTTAAGCGCCCt    <  1:345878/249‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                            ATTATCATGCGCGATCGCAAACAGGTGGCGGAGATCCCGCTGGCAGAGCTTTCCGTTCCGGCGATCATGAACGCCATTGCGGCGTAAGGAGAACAGTGTGATGCCTCAATCTCTTCCGGACACCACTACGCCAAAAAGGCGCTTTCGCTGGCCGACGGGGATGCCGCAGCTGGTAGCACTATTGCTGGTGCTGCTGGTCGATAGCCTGGTAGCCCCGCATTTCTGGCAGGTGGTGCTTCAGGATGGGCGTTTGTTCGGTAGCCCCATAGACATTCTTAACCGTGCAGCTCCCGTTGCGTTACTGGCGATTGGCATGACGCTGGTGATC‑GCCACCGGTGGGATTGATCTCTCCGTAGGGGCGGTGATGGCTATCGCCGGAGCCACAACGGCTGCGATGACGGTCGCGGGATTCAGCCTGCCGATTGTTTTGTTAAGCGCCCTGG  >  NC_000913/4452474‑4452916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑