| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,233,669 | G→A | S350F (TCT→TTT) | nhaB ← | Na(+):H(+) antiporter NhaB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,233,669 | 0 | G | A | 97.6% | 132.5 / NA | 43 | S350F (TCT→TTT) | nhaB | Na(+):H(+) antiporter NhaB |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (23/18); total (24/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGT‑TCCGATGCCTGCAACACAAACTGAATAATTGGCGAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAGATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCACCAGCCAGACGCCAATAATCGCCTGGACAATCAGACGGATTTTATCCTGACGGGTACGCTGGTGGCGGCTTTGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTT > NC_000913/1233465‑1233908 | gCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGT‑TCCGATGCCTGCAACACACACTGAATAATTGGCGAACACAGTTGTTGGTCGATAATCCCCGCGACTACCGAGAAAAACCCCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCa > 2:314009/1‑231 (MQ=255)gCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGT‑TCCGATGCCTGCAACACAAACTGAATAATTGGCGAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCa < 1:314009/231‑1 (MQ=255) cGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGT‑TCCGATGCCTGCAACACAAACTGAATAATGGGCGAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAatg < 1:313336/251‑1 (MQ=255) gCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGT‑TCCGATGCCTGCAACACAAACTGAATAATTGGCGAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAatga < 1:196471/251‑1 (MQ=255) ataAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGT‑TCCGATGCCTGCAACACAAACTGAATAATTGGCGAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGa < 1:45982/250‑1 (MQ=255) aaaTCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGT‑TCCGATGCCTGCAACACAAACTGAATAATTGGCGAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGaat > 1:229829/1‑250 (MQ=255) ccccactgaGACGTTAACCGAATTGGATGAAAGCGGACTGTCGAAAAACGAGAGCCACGACAGCTGAGCA‑GTGTCCGTGGACTGCAACACAAACTTAATAATTGGCGAAATTCGTATTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCTTCAACAGTGCGGTGAATGGCAGAAATTCGTTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAAtgtctg < 2:254185/243‑1 (MQ=255) ccccACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGT‑TCCGATGCCTGCAACACAAACTGAATAATTGGCGAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAAtgactg > 1:64460/1‑251 (MQ=255) cGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGT‑TCCGATGCCTGCAACACAAACTGAATAATTGGCGAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCa > 1:191607/1‑250 (MQ=255) gAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGT‑TCCGATGCCTGCAACACAAACTGAATAATTGGCGAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAAc > 1:347600/1‑251 (MQ=255) aTGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGT‑TCCGATGCCTGCAAAACAAACTGACTAATTGGCGAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGCCACCGGTAAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGcc > 1:48034/1‑251 (MQ=255) ggAAAATATAGAACAGCGACAGCTGAGCATGT‑TCCGATTCCTGCAACACAAACTGAATAATTGGCGAAAACAGTTGTTGGTAGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAg < 2:134856/249‑1 (MQ=255) tataGAACAGCGACAGCTGAGCATGT‑TCCGATGCCTGCAACACAAACTGAATAATTGGCGAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTc > 1:238599/1‑251 (MQ=255) ataGAACAGCGACAGCTGATCATGT‑CCCGATGCCTGCAACACAAACGGAATAATTGGCGAAAACAGTTGTTGGTCGATAAACACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCa < 2:347600/251‑1 (MQ=255) aGCGACAGCTGAGCATGT‑TCCGATGCCTGCAACACAAACTGAATAATTGGCGAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCAccagcc > 1:249497/1‑250 (MQ=255) gACAGCTGAGCATGT‑TCCGATGCCTGCAACACAAACTGAATAATTGGCGAAAACAGTTGTTGGTCGATAATAACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCACCAGCAAGAc > 1:207606/1‑251 (MQ=255) gACAACTGAGCATGT‑TCCGATGCCTGCAACAAAGAGTGAATAATTGGCGAAAACAGTTGTTGGTCGATAATCACCGAGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAGATTCGGTGAAGGCTTTACCGATAGCAGGCTCATCGGAGACACCGGTCAATGAGGTTGCCAGAATAATAACTGACAACACAATCAAGCGAAATTCCGC‑AGACAGCAACGCCAGCGCAGACAccagcccaac > 2:98769/1‑247 (MQ=255) cATGT‑TCCGATGCCTGCAACCCAAACTTAATAATTGGCGAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCACCAGCCAGACTCCAATAATc < 1:5288/251‑1 (MQ=255) ccGATGCCTGCAACACAAACTGAATAATTGGCGAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAGACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCACCAGCCAGACGCCAATAATCGCCTgg > 1:159236/1‑251 (MQ=255) aTGCCTGCAACACAAACTGAATAATTGGCGAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCgg < 1:169441/147‑1 (MQ=255) aTGCCTGCAACACAAACTGAATAATTGGCGAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGAGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCgg > 2:169441/1‑147 (MQ=255) cTGCAACACAAACTGAATAATTGGCGAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGAAACCGGTCAATGATGTTGCCAGAATAATGACTACCAAACCAATCAAGCCACCTTCCGCCAGA‑TGCACCGCCAGCGCAGTCACCAGCCAGACGCCAATAATCGCCTGGACAATcc > 2:21545/1‑250 (MQ=255) tGCAACACAAACTGAATAATTGGCGAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAACGCAGTCACCAGCCAGACGCCATTAATCGCCTGGACAATc > 1:113002/1‑249 (MQ=255) aaaCTGGATAAATGGCGAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCTGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCAGCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCTCCAGCCAGACGCCAATAATCGCCTGGACAATCAGACGGAttt < 2:9629/251‑1 (MQ=255) ttGGCGAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCACCAGCCAGACGCCAATAATCGCCTGGACAATCAGACGGATTTTATCCTGACgg > 1:345650/1‑251 (MQ=255) gAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCACCAGCCAGACGCCAATAATCGCCTGGACAATCAGACGGATTTTATCCTGACGGGTACg > 1:168416/1‑251 (MQ=255) aaCAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCACCAGCCAGACGCCAATAATCGCCTGGACAATCAGACGGATTTTATCCGGACGGGTACGCtg < 2:345650/251‑1 (MQ=255) aCAGTTGTTGGACGATAGTCACCGCGACTACCGCGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATAGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCACCAGCCAGACGCCAATAATCGCCTGGACAATCAGACGGCTTTTATCCTGACGGGTACGCtgg > 1:183493/1‑251 (MQ=255) tgTCGATAAACACCGCGACCACCGAGAAAACCACCGTCAACAGTTCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCACCAGCCAGGCGCCAATAATCGCCTGGACAATCAGACGGATTTTATCCTGACGGGTACGCTGGTGGCGGCt < 1:164475/249‑1 (MQ=255) agcACCGCGACTACCGAGAAAAACAACGTCAACAGTGCGGTGAATGGCAGAAATTCTGTGAAGGCTTTACTGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGACAGAATAATGACTGACAAACCAAACAAGCCATCTTCCGCCAGA‑TGCAACGCCAGCGCAGTCACCAGCCAGACGCCAATAATCGCCTGGACAATCAGACGGATTATATCCTGACGGGTACGCTGGTGGCGGCTGTGATCggc < 2:234019/249‑3 (MQ=255) aTCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCACCAGCCAGACGCCAATAATCGCCTGGACAATCAGACGGATTTTATCCTGACGGGTACTCTGGTGGCGGCTTTGATCGTc < 1:93172/251‑1 (MQ=255) tCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑Tg > 1:182741/1‑154 (MQ=255) tCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAAGCAAGCCAACTTCCGCCAGA‑Tg < 2:182741/154‑1 (MQ=255) aCCGCGACTACCGAGAAAAACACCGCCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCACCAGCCAGACGCCAATAATCGCCTGGACAATCAGACGGATTTTATCCTGACGGGAACGCTGGTGGCGGCTTTGATCGTCaa > 1:102550/1‑250 (MQ=255) gACTACCGCGAAAAACACCGTCAACAGTGCGGTGAATGGCAGCAATTCGGAGAAGCCTTTACCGATAGCATGCTCATCGGAGACACCGGTCAATGATGTTGCCAGAATAATGACTGAAAAACCAATCAAGCCAACTTCCGCCAGA‑AGCAACGCCAGCGCAGTCACCAGCCAGACGCCAAAAATCGCCTGGCCAATCAGACGGATTTTATCCTGACGGGTACACTGGTGGCGGCtatgctcgccaaccattt > 2:248602/1‑234 (MQ=255) cTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCACCAGCCAGACGCCAATAATCGCCTGGACAATCAGACGGATTTTATCCTGACGGGTACGCTGGTGGCGGCTTTGATCGTCAAACTGTTGc > 1:164512/1‑251 (MQ=255) aCCGATAAAAACACCGTGAACAGGGCGGGGAATGGCAGATATGCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCTGTCAATGATGTTGCCAGAATAAGGACTAACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCTGCGCAGTCTCCTGCCAGACGCCAATAATCGCCTGGACAATCAGGCGGATGTTATCCTGACGGGTACGCGGGTGGCGGCTTTGATCGTCAAACTGTAGCaa < 2:34777/251‑1 (MQ=255) ccGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCACCAGCCAGACGCCAATAATCGCCTGGACAATCAGACGGATTTTATCCTGACGGGTACGCTGGTGGCGGCTTTGATCGTCCAACTGTTGCAac > 2:347087/1‑251 (MQ=255) aaCACCGTCAACAGTGCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCACCAGCCAGACGCCAATAATCGCCTGGACAATCAGACGGATTTTATCCTGACGGGTACGCTGGTGGCGGCTTTGATCGTCAAACTGTTGCAACACTTcgcg > 1:91231/1‑251 (MQ=255) gctgTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCACCAGCCAGACGCCAATAATCGCCTGGACAATCAGACGGATTTTATCCTGACGGGTACGCtg < 2:300507/194‑1 (MQ=255) gCGGTGAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCACCAGCCAGACGCCAATAATCGCCTGGACAATCAGACGGATTTTATCCTGACGGGTACGCtg > 1:300507/1‑197 (MQ=255) gAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCTGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCACCAGCCAGACGCCAATAATCGCCTGGACAATCAGACGGATTTTATCCTGACGGGTACGCTGGTGGCGGCTTTGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGtt < 2:168416/250‑1 (MQ=255) gAATGGCAGAAATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAAGGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCACCAGTCAGACGCCAATAATCGCCTGGACAATCAGACGGATTTTATCCTGACGGGTACGCTGGTGGCGGCTTTGATCGTCAACCTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGtt < 1:221979/250‑1 (MQ=255) | GCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGT‑TCCGATGCCTGCAACACAAACTGAATAATTGGCGAAAACAGTTGTTGGTCGATAATCACCGCGACTACCGAGAAAAACACCGTCAACAGTGCGGTGAATGGCAGAGATTCGGTGAAGGCTTTACCGATAGCATGCTCATCGGTGACACCGGTCAATGATGTTGCCAGAATAATGACTGACAAACCAATCAAGCCAACTTCCGCCAGA‑TGCAACGCCAGCGCAGTCACCAGCCAGACGCCAATAATCGCCTGGACAATCAGACGGATTTTATCCTGACGGGTACGCTGGTGGCGGCTTTGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTT > NC_000913/1233465‑1233908 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |