Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,987,499	C→A	intergenic (+71/+8)	ftnB → / ← yecJ	putative ferritin‑like protein/DUF2766 domain‑containing protein YecJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,987,499	0	C	A	100.0%	37.5 / NA	15	intergenic (+71/+8)	ftnB/yecJ	putative ferritin‑like protein/DUF2766 domain‑containing protein YecJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (7/8);  total (7/8)

ATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAATTTGTTTGATAACCAACTGGCAAGCAACCACATCAGAAACCA  >  NC_000913/1987277‑1987727                                                                                                                                                                                                                               |                                                                                                                                                                                                                                     aTTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTTCGCCGATGAAGGTGTTGAGAAACCGCTCCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTgg                                                                                                                                                                                                          <  1:310617/251‑1 (MQ=255)    tcatGCGCGATCTGGAAAAAGAACTGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTAATGAAGTGCGCAGTGCGAAACTTGCGGGTATGGGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACCCCATCTGCATTGATCATCAACGGCGCTAATGAATTGCGCCGATGAAGGGTTTGAGAAACAGCTGCCTCTTCTGTTTGAAGCAGAAGATTTTTTAAGGGGCTCCACCTTGGGGGGt                                                                                                                                                                                                       >  2:123015/4‑251 (MQ=255)                    aaaaGAACGGCAGCATGATGGTCTGTCGCTGCTAAACATTCTTGATGACGTACGCAGTGCGCAACTTGCGGGTATGCGCCCTGTGCAAACCGACCCACATGTTCTGAATGGCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGAACTTCGCCGATGAAGGTTTTGAGATACCGCTGCCTCATCTGTTTGATGCCGAGGTTTTTTTAATGGTATTCACCCTGGTGGGTAAACTTGAGTTCAAt                                                                                                                                                                                        >  2:53538/1‑250 (MQ=255)                                              tgctgaAAACCATTCGTGATGAAGGGCGCAGTGCGAAACTCGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGGCGTGTCACCCCAGCTTCAGTGATCATCACCGGCGCTAATGCATTGCGGCGATGAAGGTTTTGAGAATCCGCTGCCTCATCTGTTTGAAGCATAGGCTTTTTTAATGTGATTCACCCTGTGGGGTAAACTTGAGTGCAATAAGCGCGAAGGCTGTGTGGATTGccc                                                                                                                                                              <  2:331837/250‑1 (MQ=255)                                                                                                                                tgacGTGTCACACCAGCTGCATTGATCATCATCGGCGCTACTGCATTGGCCCGATAAAGGTTTTGAGAAACCGCTGACTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGGAAACTTGAGTTCAATAAGCGCGATAGCTTTTTGGATTGCCCGCATGGTGACCGGGTATGCGGCGGCGGGATGGTTAGTAAAGTAGATATTCTTCAGCTGACTGGACATTTTTTCAAGCACTTc                                                                            >  1:292491/4‑250 (MQ=255)                                                                                                                                       acacaCCAGCTGCATTGATCATCATCGGCGCTAATGCAGTGCGCCGATGAAGGGTTGGAGAAATCGTTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTTGGGGGTAAACTTGAGTTCTATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGgcg                                                                    <  2:117231/250‑1 (MQ=255)                                                                                                                                        cacaCCATCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTTAGAAACCGCTGCCTCATCTGTTTGAAGTAGATGTTTTTGTAATGGGATTCTCCTTTTGGGGTAAACTTGAGTTCAATGAGCGCGATGGCTGTTTTGATTGCCCGCATGGTGACCGGGTCTGCGGCTGCGGGATGTTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGgcg                                                                    <  1:314192/250‑1 (MQ=255)                                                                                                                                                      tGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCgaga                                                      >  1:65891/1‑250 (MQ=255)                                                                                                                                                       gATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGTTGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACTAACTTCACAGGGCGCGATTACATCGAGAAc                                                    >  2:79508/1‑251 (MQ=255)                                                                                                                                                            tcatcGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCAGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTCGTAAAGTCGATATTCTTCAGATGACTGGACCTTTTTTCACGCACTTCAACGGGAGCGATTCCATCGAGAACATcc                                                >  2:308232/1‑250 (MQ=255)                                                                                                                                                                  gcgcTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAAttt                                         >  1:305704/1‑251 (MQ=255)                                                                                                                                                                       aaTGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAAtttgtt                                      <  1:23038/249‑1 (MQ=255)                                                                                                                                                                          gCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAATTTGTTTGATa                                 <  1:80887/251‑1 (MQ=255)                                                                                                                                                                              tGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGAGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAATTTGTTTGATAAcc                              <  1:278508/250‑1 (MQ=255)                                                                                                                                                                                                           gCCTTATCTGTTTGAAGCAGAGGATTTTTTAATTGGATTCACCCTGTGGGGTAAACGTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCAGGGTGACCTGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATGCTTCAGCTGACTGTACAGTTTTTCACGAACTGCAACGGGCGCGATTACATCGAGAACATCCAGAATTTGTTTGATAACCAACTGGCAAGCAACCACATCAGAAACCa  <  1:176312/249‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                     ATTTCCTGCGCGATCTGGAAAAAGAACAGCAGCATGATGGTCTGTTGCTGCAAACCATTCTTGATGAAGTGCGCAGTGCGAAACTTGCGGGTATGTGCCCTGTGCAGACCGACCAACATGTTCTGAATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTGAGTTCAATAAGCGCGATGGCTTTTTGGATTGCCCGCATGGTGACCGGGTCTGCGGCGGCGGGATGGTTAGTAAAGTCGATATTCTTCAGCTGACTGGACATTTTTTCACGAACTTCAACGGGCGCGATTACATCGAGAACATCCAGAATTTGTTTGATAACCAACTGGCAAGCAACCACATCAGAAACCA  >  NC_000913/1987277‑1987727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑