Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,783,983	G→A	S49F (TCC→TTC)	secB ←	protein export chaperone SecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,783,983	0	G	A	97.2%	104.6 / NA	36	S49F (TCC→TTC)	secB	protein export chaperone SecB
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (18/17);  total (19/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCAGTTGCGGGAATGTACCGCGGGATACCATGCTGGTGATGCACTCACGAGCATACGGGAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCC‑TTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAAACCCTTAAGTGTTGTCCTTAAATACTGCGCAATGTAATGCCAACGTTCACACGTTATTTCAGCAGGGGATCCAGTCCACCACGTGCATCC  >  NC_000913/3783749‑3784227                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      ttCAGTTGCGGGAATGTACCGCGGGATACCATGCTGGTGATGCACTCACGAGCATACGGGAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATcccg                                                                                                                                                                                                                                       >  1:346885/1‑249 (MQ=255)    aGTTGCGGGAATGTACCGCGGGATCCGCTGCTGGTGATGCACCCACGAGCATACGGGAAAAGAATGTTCGGGCAGCATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGCAAATACCGACCTGCTGAACTTCACCCAGGAACGCGGTATCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCAAGTTGGAAAGATGCCGTAtac                                                                                                                                                                                                                                         >  1:310983/1‑244 (MQ=255)           ggAATGTACCGCGGGATACCATGCTGGTGATGCACTCACGAGCATACGGGAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTa                                                                                                                                                                                                                             <  1:237302/251‑1 (MQ=255)                gTACCGCGGGATACCATGCTGGTGATGCACTCACGAGCATACGGGAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTc                                                                                                                                                                                                                        >  1:170191/1‑251 (MQ=255)                        ggctACCATGCTGGTGATGCACTCACGAGCATACGGGAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCCAGTTTAACTTCTGGTTGcc                                                                                                                                                                                                                >  1:21127/4‑251 (MQ=255)                                tGCTGGTGATGCACTCACGAGCATACGGGAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTc                                                                                                                                                                                                        >  1:258242/1‑251 (MQ=255)                                   tGGTGATGCACTCACGAGCATACGGGAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTgg                                                                                                                                                                                                     >  2:245287/1‑251 (MQ=255)                                          gCACTCACGAGCATACGGGAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACg                                                                                                                                                                                               <  1:238175/250‑1 (MQ=255)                                                         cGGGAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGCGGCCGTTACGGTAACACGCAGTACAACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCTAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGgcg                                                                                                                                                                               >  1:100615/1‑251 (MQ=255)                                                            gAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACAAGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCtt                                                                                                                                                                            >  2:73914/1‑251 (MQ=255)                                                                  aacgtccgggtagtcagCTCCCAGGCAATGCCCTATCTCTGTGACTGCGATACCCGCGAGTGATAAAATACCGCCCGGCTGCACTTCACACAGGAACGCGGTGTCTTCTCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAACAATGCCGTATCCAGATCAATTGTAACTGCTGGTCGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCTCttataaag                                                                                                                                                                      <  2:123772/235‑7 (MQ=255)                                                                  aaTGTTCGGGCAGTATGATCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCATTCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGaa                                                                                                                                                                        >  1:64328/1‑249 (MQ=255)                                                                        cGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGatat                                                                                                                                                                >  1:30449/1‑251 (MQ=255)                                                                         ggtcAGTATGTTTCCAGGCTATTCGCCCTCTTTGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGGACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTTACTTCTGGTTGCCAAACTTTCTGGAAAACGTGCGGCGCGGTCGGCGCTTCGAAAGAGATATc                                                                                                                                                               <  2:46559/248‑1 (MQ=255)                                                                                                cgcCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCC‑TTGGTATAAATACGTTGGATCt                                                                                                                                       >  1:208636/1‑251 (MQ=255)                                                                                                                tCGATACCCGCGAGGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCC‑TTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAg                                                                                                                        <  2:120579/250‑1 (MQ=255)                                                                                                                 cGATACCCGCGATGGAGAAAATACCGCCCTGCTGCACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCA‑TTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAgtg                                                                                                                      >  2:36991/1‑251 (MQ=255)                                                                                                                      cccGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTGTCTTCGCCCAAAGAGGCCGGTACGGTAACACGCAGTACCACTTCGTATCCGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACGTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCC‑TTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTgttgtt                                                                                                                 <  2:258242/251‑1 (MQ=255)                                                                                                                            aTGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCC‑TTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTc                                                                                                           >  1:332817/1‑251 (MQ=255)                                                                                                                                         cGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCg                                                                                                                                                                          <  2:135469/176‑1 (MQ=255)                                                                                                                                         cGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCg                                                                                                                                                                          >  1:135469/1‑176 (MQ=255)                                                                                                                                                           acaGAAACGCGGTTTCTCCGCCCAAAGAGGCCGTGCCGGTAACAAGCAGTACCACATCGTATACGTGATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCC‑TTTGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAAACCCTTAAGTGTTGTc                                                                            <  2:257593/251‑1 (MQ=255)                                                                                                                                                            caGGGACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACGTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCC‑TTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAAACCCTTAAGTGTTGTcc                                                                           <  2:61444/251‑1 (MQ=255)                                                                                                                                                                    cgGTTTCTTCACTCAAAGAGGCCGGTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCATTTGGAATGATGCCGTATCCCGATCAAGTTTAACTTCTGCTTGCCAATCTTTCTGGAAAACGTGCGGCTCGTTCGGCGTTTCGAAAGATATGTCCGTTGG‑ATATATACATTAGGTCTTGAAAGTCAATTCAGTGATATTATGTTCTGACATGTGTAGAAAACCCTTAAGTGAGGTCCTTAAATAc                                                                   <  2:18472/251‑1 (MQ=255)                                                                                                                                                                      gTTTCTTCCCCCAAAGAGTCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGAGATCC‑TTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAAACCCTTAAGTGTTGTCCTTAAATACTg                                                                 <  2:30449/251‑1 (MQ=255)                                                                                                                                                                                       ggCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCC‑TTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAAACCCTTAAGTGTTGTCCTTAAATACTGCGCAATGTAATGCCAAc                                                >  1:194898/1‑251 (MQ=255)                                                                                                                                                                                                      acGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTGAACTTCTGGTTGCCAATCTTTCTGGAAACCGTGCGGCGCGTTCGGCTCTTCGAAAGAGATATCC‑TTGGTATAAATACGTGGTATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAAACCCTTAAGTGTTGTCCTTAAATACTGCGCAATGTAATGCCAACGTTCACACGTTAttt                                 <  1:328268/251‑1 (MQ=255)                                                                                                                                                                                                         ccgTACCACTTCGTATCCGTCATCTGCCAGTTTGACATCTTCCTTATCCATATCAAGTTTCACTTCTGTTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAGGAGATATCC‑TTGGTATAAATACGTTGTATCTTGAACATCATTTCCGTGTTGTTTTGTTCTGACATGATTATACAACCCTTAACTGTTGTCCTTAAATACTACGAAATGTACTGCCAACTTTCACACTTTATTTccg                              >  2:38713/3‑249 (MQ=255)                                                                                                                                                                                                                 ctgcGTAAACGTCATCTGCTAGTTGGAAAGATGCCGTATCCATATCAAGTTTAACTTCTGGTTGCCAATTTTTCTGGAAAACGTGCGGCGCGTTCGGCTCTTCGAAAGAGATATCC‑TTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAAACCCTTAAGTGTTGTCCTTAAATACTGCGCAATGTAATGCCAACGTTCACACGTTATTTCAGCAgggg                        <  2:305714/246‑1 (MQ=255)                                                                                                                                                                                                                    cGTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCC‑TTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATTTTTAGAAAACTCTTAAGTGTTGTCCTTAAATACTGCGCAATGTAATGCCAACGTTCACACGTTATTTCAGCAGGGGATcc                    <  1:158858/250‑1 (MQ=255)                                                                                                                                                                                                                     gTATACGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCC‑TTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAAACCCTTAAGTGTTGTCCTTAAATACTGCGCAATGTAATGCCAACGTTCACACGTTATTTCAGCAGGGGATcc                    >  1:104952/1‑249 (MQ=255)                                                                                                                                                                                                                       ataCGTCATCTGCCAGTTGGAAAGATGCCTGATCCATATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCC‑TTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAAACCCTTAAGTGTTGTCCTTAAATACTGCGCAATGTAATGCCAACGTTCACTCGTTATTTCAGCAGGGGATCCAGt                 <  1:128883/250‑1 (MQ=255)                                                                                                                                                                                                                         aCGTCATCTGCCAGTTGGAAAGATGCCGGATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTTCGGCGCGTTCGGCGCTTCGAAAGAGATATCC‑GTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAAACCCTTAAGTGTTGTCCTTAAATACTGCGCAATGTAATGCCAACGTTCACACGTTATTTCAGCAGGGGATCCAGTcc               <  2:346885/250‑1 (MQ=255)                                                                                                                                                                                                                          cGTCATCTGCCAGTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCC‑TTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAAACCCTTAAGTGTTGTCCTTAAATACTGCGCAATGTAATGCCAACGTTCACACGTTATTTCAGCAGGGGATCCAGTccac             >  1:55304/1‑251 (MQ=255)                                                                                                                                                                                                                            tCATGTGCCAGTTGGAAAAATGTCGTCTCCAGAACAAGTGTAGCGTCTGGTTTCCAATCTTTCTGGAAAACGTGCGGCGCGTGCGGCGATTCGAAAGAGATAGCC‑TTGGTATTAATACGTGGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGGGTAGAAAACCCTTAAGTGTTGTCCTTAAATACTGCGCAATTTAATGCCAACATTCGCACTTTATTTCAGCAGTGTATCCAGTccacc            <  2:317206/250‑1 (MQ=255)                                                                                                                                                                                                                                      gTTGGAAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCC‑TTGGCATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAAACCCTTAAGTGTTGTCCTTAAATACTGCGCAATGTAATGCCAACGTTCACACGTTATTTCACCAGGGGATCCAGTCCACCACGTGCATcc  >  1:59159/1‑250 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      TTCAGTTGCGGGAATGTACCGCGGGATACCATGCTGGTGATGCACTCACGAGCATACGGGAACAGAATGTTCGGGCAGTATGCTCCCAGGCAATGCGCCATCTGGGTGCCTTCGATACCCGCGATGGAGAAAATACCGCCCTGCTGAACTTCACACAGGAACGCGGTTTCTTCGCCCAAAGAGGCCGTTACGGTAACACGCAGTACCACTTCGTATACGTCATCTGCCAGTTGGGAAGATGCCGTATCCAGATCAAGTTTAACTTCTGGTTGCCAATCTTTCTGGAAAACGTGCGGCGCGTTCGGCGCTTCGAAAGAGATATCC‑TTGGTATAAATACGTTGGATCTGGAAAGTCATTTCAGTGTTGTTTTGTTCTGACATGTGTAGAAAACCCTTAAGTGTTGTCCTTAAATACTGCGCAATGTAATGCCAACGTTCACACGTTATTTCAGCAGGGGATCCAGTCCACCACGTGCATCC  >  NC_000913/3783749‑3784227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑