Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,652,331	T→C	intergenic (+2346/+397)	ydfD → / ← ynfP	lysis protein/protein YnfP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,652,331	0	T	C	100.0%	38.7 / NA	12	intergenic (+2346/+397)	ydfD/ynfP	lysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/6);  total (6/6)

GTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGT‑ATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATGC  >  NC_000913/1652100‑1652575                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      gTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACTCATGCGGTAACCAATCGGGTCATTCATCTTATTGAGCCAGCAATCGGCGCTCTCCGGAGTCAGATGGCATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGATT‑ATGGcag                                                                                                                                                                                                                                      <  1:229185/248‑1 (MQ=255)    tataCCGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGT‑ATGGcagaacag                                                                                                                                                                                                                                 <  1:171653/250‑1 (MQ=255)           gAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGGGTCAGATGACATTAACGAGAATGAGCAAAGAGCATATCATTGATGTTCAACTCAGAGAGG‑ATGGAAGAAAAGAAAAACa                                                                                                                                                                                                                          >  1:191249/1‑250 (MQ=255)                cGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGT‑ATGGCAGAACAGAAAAACAAAAATg                                                                                                                                                                                                                    <  1:287873/251‑1 (MQ=255)                                gtgCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGGCATTCATCTTATTAAGCCAGCAAACGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGG‑ATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCa                                                                                                                                                                                                     >  1:122787/1‑250 (MQ=255)                                                                        tatgatgATCAGGAGAAATTTATCAAAAGTCCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGT‑ATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCgaga                                                                                                                                                                                  <  1:166913/228‑1 (MQ=255)                                                                                              gCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCTGGTCCTTCCTCTTATTAAACCAGCCATCGACGCTCTCCGGAGTCAGGTTAAATTAAAGAGACTGAGCAAAGAGAATATCATTGATGTTCACCTCAGAGAGTCATG‑CAGAACAGACAAACAAAAATTAACCTTTTTTTTTCAACCTTAATTGTAAACGAGAGTAAAAAAATATAGTGAACATTTTATCGTTgtctaccaatggc                                                                                                                                       >  2:279659/1‑238 (MQ=255)                                                                                                    gACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGT‑ATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGAtgtg                                                                                                                                <  1:84137/251‑1 (MQ=255)                                                                                                                                    caCCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGCCTGAGCAAAGAGCATATCATTGATGTTCACCTCAGAGAGT‑ATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACAGTTGACTCAATAAGGCCGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGcc                                                                                                >  2:201127/2‑251 (MQ=255)                                                                                                                                                                 cAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAACAAAGAGCATATCATTGATGTTCACCTCAGAGAGT‑ATGGCAGAACAGAAAACCAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAACTCATATCAGTCGAGACATACt                                                                   >  1:254404/1‑251 (MQ=255)                                                                                                                                                                                                                                  gTTCACCTCAGAGGGT‑ATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGGCGAGACATACTTATGCCTGGTGGTCGATGACAGCTGGGGCTAACCCGGCATTTATAGCaaccaagaagggacctgg  >  2:99234/1‑235 (MQ=255)                                                                                                                                                                                                                                  gTTCACCTCAGAGAGT‑ATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATGc  <  1:279659/251‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      GTTTATACTGGAGTTCGCCCTGGTGAGCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACTTCAGAGAGT‑ATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGGCCATGC  >  NC_000913/1652100‑1652575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑