Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,330,263	A→G	intergenic (‑466/+4073)	yfaQ ← / ← yfaT	tandem DUF2300 domain‑containing protein YfaQ/DUF1175 domain‑containing protein YfaT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,330,263	0	A	G	100.0%	87.2 / NA	28	intergenic (‑466/+4073)	yfaQ/yfaT	tandem DUF2300 domain‑containing protein YfaQ/DUF1175 domain‑containing protein YfaT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/14);  total (14/14)

GGTTTATTGACCGAAATGCCCCATGTTGTGCGCTCAACGTCGGCTCCCGGTGGCAGCGGTACTTCCACCTGACCGTAGCGCAGAACTGCATCCTGCTCGCTGGTAAGCGTGATTTCATCGAGATACAGCGCATCGCTGTCAATCTCATTGCTGGTCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAAAGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTATGTTTAGCC‑CATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTG  >  NC_000913/2330033‑2330503                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  ggTTTATTGATCGTAATGACCCATGTTGTGCGATCAACGCATGCTCCCGGTGGCAGCGGTACTTCCTCCTTGCCGAAGCGCAGAACTGCATCCTGCTCGCTGGTAAGAGTGATTTCATCGAGATACAGCGCATCGCTGTCAATCTCATTGCTGGTCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCAACAGGGATAAGCCGATACAACTGGCGTTAAACGGTCACCGGGATGTTACTTTGTTGAGCCAt                                                                                                                                                                                                                               <  2:264558/251‑1 (MQ=255) ggTTTATTGACCGAAATGCCCCATGTTGTGCGCTCAACGTCGGCTCCCGGTGGCAGCGGTACTTCCACCTGACCGTAGCGCAGAACTGCATCCTGCTCGCTGGTAAGCGTGATTTCATCGAGATACAGCGCATCGCTGTCAATCTCATTGCTGGTCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTTGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCAt                                                                                                                                                                                                                               <  1:262836/251‑1 (MQ=255)             gcaaTGCCCCATGTTGTGCGCTCAACGTCGGCTCCAGGTAGAAGCGGTACTTCCACCTGACCGAAGCGCAGAACTGCATACTGCTCGATGGTAAGCGTGATTTCATCGCGATACAGCGCATCGCTGTCAATCTCATTGCTGGTCACCGGTTGCAGAATACAGCTCATCTCTTCTTCACCAGGGATAAGCCGCTACAACTGGCGTTAAACGGTCACCGGGATGTTACTTTGTTGGGCCATTTTTTCCGGCTc                                                                                                                                                                                                                   >  2:202558/3‑251 (MQ=255)              aaaTGCCCCATGTTGTGCGCTCAACGTCGGCTCCCGGTGGCAGCGGTACTTCCACCTGACCGTAGCGCAGAACTGCATCCTGCTCGCTGGTAAGCGTGATTTCATCGAGATACAGCGCATCGCTGTCAATCTCATTGCTGGTCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTAACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTc                                                                                                                                                                                                                   >  2:301664/1‑250 (MQ=255)                                       gTCGGCTCCCGGTGGCAGCGGTACTTCCACCTGACCGTAGCGCAGAACTGCATCCTGCTCGCTGGTAAGCGTGATTTCATCGAGATACAGCGCATCGCTGTCAATCTCAGTGCTGGTCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTgcg                                                                                                                                                                                         <  1:332141/251‑1 (MQ=255)                                         cGGCTCCCGGTGGCAGCGGTACTTCCACCTGACCGTAGCGCAGAACTGCATCCTGCTCGCTGGTAAGCGTGATTTCATCGAGATACAGCGCATCGCTGTCAATCTCATTGCTGGTCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTgcggc                                                                                                                                                                                       >  2:248186/1‑251 (MQ=255)                                              cccGGTGGCAGCGGTACTTCCACCTGACCGTAGCGCAGAACTGCATCCTGCTCGCTGGTAATCGTGATTTTATCGATATACAGCGCATCGCTGTCAATCTCATGGCCGGTCCCCGGTTGCATAATAAAGCCAATCTCTTCCTCACCATGGATAAGCCGATACAACTTGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCGGCACATTTTGCGGCGAt                                                                                                                                                                                    <  2:317417/249‑1 (MQ=255)                                               ccGGTGGCAGCGGAACTTCCACCTGACCGTAGCGCAGAACTGCATCCTGCTCGCTGGTAAGCGTGATTTCATCGAGATACAGCGCATCGCTGTCAATCTCATTGCTGGTCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGCTAAt                                                                                                                                                                                 >  2:312982/1‑251 (MQ=255)                                                  gTGGCAGCGGTACTTCCACCTGCCCGTAGCGCAGAACTGCATCCTGCTCGCTGGTAAGCGTGATTTCATCGAGATACAGCGCATCGCTGTCAATCTCATTGCTGGTCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACCTTTTGCGGCGATAATTc                                                                                                                                                                               >  2:207168/1‑250 (MQ=255)                                                  gTGGCAGCGGTACTTCCACCTGACCGTAGCGCAGAACTGCATCCTGCTCGCTGGTAAGCGTGATTTCATCGAGATACAGCGCATCGCTGTCAATCTCATTGCTGGTCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTc                                                                                                                                                                               <  1:207168/250‑1 (MQ=255)                                                                tCCACCTGACCGTAGCGCAGAACTGCATCCTGCGCGCTGGTAAGCGTGATTTCATCGAGATACAGCGCATCGCTGTCAATCTCATTGCTGGTCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAAagag                                                                                                                                                                  <  1:198945/249‑1 (MQ=255)                                                                     cTGACCGTAGCGCAGAACTACATCCTGCTCGCTAGTAAGCGTGATTTCATCGAGCTAAAGCGCATCGCTGTCAATCTCATTGCTGGTCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACACCTGGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGACTCACGTCATCGGACCTGCACATTTTGCGGCGATAATTCGTCACCCAAAGAGAGAATg                                                                                                                                                            >  2:151730/1‑250 (MQ=255)                                                                                       tGCATCCTGCTCGCTGGTAAGCGTGATTTCATCGAGATACAGCGCATCGCTGTCAATCTCATTGCTGGTCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAAAGAGAGAATGTCCGGCACGCCCTGAcca                                                                                                                                          >  1:165493/1‑250 (MQ=255)                                                                                          aTCCTGCTCGCTGGTAAGCGTGATTTCATCGAGATACAGCGCATCGCTGTCAATCTCATTGCTGGTCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAAAGAGAGAATGTCCGGCACGCCCTGAccaaccc                                                                                                                                      >  1:6069/1‑251 (MQ=255)                                                                                                cTCGCTGGTAAGCGTGATTTCATCGAGATACAGCGCATCGCTGTCAATCTCATTGCTGGTCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAAAGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGcc                                                                                                                                >  1:109121/1‑251 (MQ=255)                                                                                                                  ttCATCGAGATACAGCGCATCGCTGTCAATCTCATTGCTGGTCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAAAGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCa                                                                                                               <  1:301664/250‑1 (MQ=255)                                                                                                                       cGAGATACAGCGCATCGCTGGCAATCTCATTGCTGGTCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAAAGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAAc                                                                                                         <  2:165493/251‑1 (MQ=255)                                                                                                                           aTACAGCGCATCGCTGTCAATCTCATTGCTGGTCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAAAGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTAt                                                                                                     <  1:312982/251‑1 (MQ=255)                                                                                                                                  gcATCGATGTCAATCTCATTGCTGGTCCCCGGTTGCAGAATAAAGCTCATCTCTGCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAAAGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTATGTTTAGc                                                                                              <  1:202558/251‑1 (MQ=255)                                                                                                                                            cAATCTCATTGCTGGTCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCTGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGACACCAAAAGAAAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGACTTCGCCCCCTCCAGTTACCTTATTTTTAGCC‑CATGCTccc                                                                                   >  2:288618/1‑251 (MQ=255)                                                                                                                                                      gCTGGTCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAAAGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTATGTTTAGCC‑CATGCGCCcgcaggcgcag                                                                         <  1:248186/251‑1 (MQ=255)                                                                                                                                                        tGGTCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAAAGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTATGTTTAGCC‑CATGCGCCCgcaggcgcaggc                                                                       >  1:158958/1‑251 (MQ=255)                                                                                                                                                           tCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGTTAATTCGTCACCAACAGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTACCTTATGTTTAGCC‑CATGCGCCCGCAGGCGCAGGCaa                                                                     >  2:312804/1‑250 (MQ=255)                                                                                                                                                              ccGGTTTCAGAATAAAGCTCAGCTCTTCTTCACCAGGGATAAGCCGATACAACTGTCGTGCAACGGACACCTGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAAAGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCATTCTTCGCCCCCTCCAGTTAACTTATGTTTAGCC‑CATGCGCCCGCAGGCGCAGGCAAaaca                                                                 <  1:203650/251‑1 (MQ=255)                                                                                                                                                                                                aGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATCATTCGTCACCAAACGTGAGAATGTCCGGCACGCCCTGACAAACCCAACGCCCGTCTTCGCCCCCTCCCGTTAACTTATGTTTAGCCACA‑GCGCCCGCACGCGAATGCAAAACAACTGGATGCCTTGTCGCCATATATTTcgcccgcc                               >  2:242233/1‑251 (MQ=255)                                                                                                                                                                                                      aaGCCGATACAACTGGCGTTCAACGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAAAGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTATGTTTAGCC‑CATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTc                          <  1:312804/250‑1 (MQ=255)                                                                                                                                                                                                            aTACAGCTGGCGTTCAACGGTCACTGGGATGTTACTTTGTTGAGCCAGTTTTGCCGGCTCACGTCAGCGGACCTGCACAGTTTGCGGCGATAATGCGTCACCAAAAGAGAGAATGTCCGGCATGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCGCCAGTTAACTTATGTTTAGCC‑CATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCa                   <  1:100180/251‑1 (MQ=255)                                                                                                                                                                                                                             cGGTCACCGGGATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAAAGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTATGTTTAGCC‑CATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTg  >  1:111336/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  GGTTTATTGACCGAAATGCCCCATGTTGTGCGCTCAACGTCGGCTCCCGGTGGCAGCGGTACTTCCACCTGACCGTAGCGCAGAACTGCATCCTGCTCGCTGGTAAGCGTGATTTCATCGAGATACAGCGCATCGCTGTCAATCTCATTGCTGGTCACCGGTTGCAGAATAAAGCTCATCTCTTCTTCACCAGGGATAAGCCGATACAACTGGCGTTCAACGGTCACCGGAATGTTACTTTGTTGAGCCATTTTTGCCGGCTCACGTCAGCGGACCTGCACATTTTGCGGCGATAATTCGTCACCAAAAGAGAGAATGTCCGGCACGCCCTGACCAACCCAACGCCAGTCTTCGCCCCCTCCAGTTAACTTATGTTTAGCC‑CATGCGCCCGCAGGCGCAGGCAAAACAACTGGAGGCATTGTCGCCATATATTTCGCCAGCCAGTTCATGGCCAGCGCGCGCTCAATAGTG  >  NC_000913/2330033‑2330503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑