Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,433,144	G→A	F177F (TTC→TTT)	pinR ←	putative site‑specific recombinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,433,144	0	G	A	100.0%	35.8 / NA	19	F177F (TTC→TTT)	pinR	putative site‑specific recombinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/9);  total (10/9)

AACTGCACAGGATATTGAATGGCCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATGAGTGCGTTCAAGTAAAAGGTCTCGTTCAAATTCAGCGACTGCTGAAATTACTTGCATCATCATTTTTCCTGTTGGACTGGTCAGGTCAATGCCACCCAATGCTAAGCAATG  >  NC_000913/1432950‑1433389                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      aaCTGCACAGGATATTGAATGGCCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTAAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATaccac                                                                                                                                                                                               <  1:146741/251‑1 (MQ=11)                     ggCCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTAAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCAttt                                                                                                                                                                            >  1:198978/1‑250 (MQ=14)                                                  aaaTTCTAGAATTAGATGTATCTTTCCATTTACGGAAATGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTAAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTGCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCt                                                                                                                                              <  2:30090/251‑1 (MQ=255)                                                                 aTGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCTAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTAAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCtt                                                                                                                               <  1:323888/251‑1 (MQ=18)                                                                 aTGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTAAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCtt                                                                                                                               >  1:150725/1‑251 (MQ=18)                                                                 aTGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTAAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCtt                                                                                                                               >  2:107962/1‑251 (MQ=18)                                                                   gTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGCGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTAAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTAc                                                                                                                             >  1:332198/1‑251 (MQ=18)                                                                                tACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTAAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATg                                                                                                                 >  1:269637/1‑250 (MQ=18)                                                                                                            tAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTAAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAAc                                                                                                                                                      >  2:66517/1‑185 (MQ=21)                                                                                                            tAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTAAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAAc                                                                                                                                                      <  1:66517/185‑1 (MQ=21)                                                                                                              aaGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTAAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATGAGTGCGTTCAAGTAAAAGGTCTCGTTCAAAt                                                                                  <  1:334430/251‑1 (MQ=18)                                                                                                                      cacaCCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTAAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATGAGTGCGTTCAAGTAAAAGGTCTCGTTCAAATTCAGCg                                                                            <  1:108948/249‑1 (MQ=18)                                                                                                                                     ttttATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTAAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATGAGTGCGTTCAAGTAAAAGGTCTCGTTCAAATTCAGCGACTGCTGAAATTACTTg                                                           >  1:181384/1‑251 (MQ=18)                                                                                                                                        taaatgtcatgtggttGAAGTTTTGCTTTGGCTCTTAAAATGGGTGTCCGCGAGGTTTTAAATTCCCGGGCAAGGGCACTTATACTTACAACTGACTTAATTCGTTCGAATACCACCTGTTGCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATGAGTGCGTTCAAGTAAAAGGTCTCGTTCAAATTCAGCGACTGCTGAAATTACTTGca                                                         <  2:181384/235‑1 (MQ=11)                                                                                                                                                           gTTTTGCTTTGTCTCTTAAAATAGTTTGCCGCGTGGTTTTAAATTACCGGGCAATGGATATTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTATTCATTTACCACTAGCGGTAGACCACACCGTTTCCCTTCGTCTAGCGATCTTACTATCCCGGAATGAGTACGTTCAAGTAAACGATCTCGTTCAAATTCAGCGGCTGCTGAAAATACTTACATCATCATTTTTTCTGTTgat                                     >  2:307320/1‑249 (MQ=2)                                                                                                                                                                                  gTTTGCCGCGAGGTTTTAAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTCCCCTGCGCCGCGGGCGCTTACTATCCCGGAATGAGTGCGTTCAAGTAAAAGGt                                                                                             <  1:144766/172‑1 (MQ=255)                                                                                                                                                                                           gAGGTTTTAAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGCATGAGTGCGTTCAAGTAAAAGGTCTCGTTCaa                                                                                    >  1:229538/1‑172 (MQ=21)                                                                                                                                                                                           gAGGTTTTAAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCGTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCGCTTATTATCCCGGAAGGAGCGCGTTCAAGTAAAAGGTCTCGTTCaa                                                                                    <  2:229538/172‑1 (MQ=11)                                                                                                                                                                                              gTTTTAAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATGAGTGCGTTCAAGTAAAAGGTCTCGTTCAAATTCAGCGACTGCTGAAATTACTTGCATCATCATTTTTCCTGTTGGACTGGTCAGGTCAATGCCACCCAATGCTAAGCAATg  >  1:192529/1‑251 (MQ=21)                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      AACTGCACAGGATATTGAATGGCCAGCACTGCCGTAGGGTAAAACATATAAATTCTATAATTAGATGTATCTTTCCATTTACGGCAAGGAAGGGGGCTTGGAAGACGTAAAGCATCTCACACCGAGATTATTTTTTATATGTCAGGTGTCTGAAGTTTTGCTTTGGCTCTTAAAATGGTTTGCCGCGAGGTTTTGAATTCCCGGGCAATGGCACTTATACTTACACCTGACTTAATTCGTTCGAATACCACCTGTTTCTGTTCTTCATTTAACACAGGAGGTCGACCAAAACGTTTCCCTGCGCCGCGGGCTCTTACTATCCCGGAATGAGTGCGTTCAAGTAAAAGGTCTCGTTCAAATTCAGCGACTGCTGAAATTACTTGCATCATCATTTTTCCTGTTGGACTGGTCAGGTCAATGCCACCCAATGCTAAGCAATG  >  NC_000913/1432950‑1433389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑