Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,325,644	C→T	S33N (AGC→AAC)	yjdN ←	PF06983 family protein YjdN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,325,644	0	C	T	97.2%	108.2 / NA	36	S33N (AGC→AAC)	yjdN	PF06983 family protein YjdN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (19/16);  minor base A (1/0);  total (20/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

GCCATTTCGATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGA‑GGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGG  >  NC_000913/4325398‑4325889                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        gCCATTTCGATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGCAATTGCATTCCGGAAGGGCCGTTCTCGGCGCTGTCCTGCGCTGATTATGGCATTTCGCCGTAGTTG‑At                                                                                                                                                                                                                                                    >  2:151161/1‑251 (MQ=255)                  gTTAGCGGCAAGATTGGCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCcg                                                                                                                                                                                                                                   <  1:208107/251‑1 (MQ=255)                                         cgagatttCATTCTTCGAACTGCTGCGAATCGAGCACACGCGTAACGCCGGCGTAGCTGGCTTTTCCTGACGGCATGGAATCGCTCATCATGCTGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCAGTGGAGGCATCGGGAAATTGCATTACGGTAGGGAAGTTCTCGGCGCTGACCTGCAATGTTTTTGGCATTTAGGAGAA‑ATGTGTTTTATAGTGCTGTTCCGCGCCCTACGTACGCTGacaaagg                                                                                                                                                                                                            >  2:163966/6‑245 (MQ=255)                                           cGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGAGTGGGCGATGGAGGTATAGGGTAATTGCATTCCGGAAGGGAAGTTCTAGGCGCTGTACTGAGATGATTTTGGCATTTCGCCGGAGTTG‑ATTTTATAGAGGAGTTCCGCGCCCAACGTACGGTGataagtggc                                                                                                                                                                                                          >  2:314492/1‑246 (MQ=255)                                                 ccTTCTTCGACCTGTTGCGAATCGAGCTCCAGCGTAAAGCCGGAGTAGCTGGGTTTTCCTTACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCCCATTGGCGCGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGGTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATcg                                                                                                                                                                                                     <  1:312319/250‑1 (MQ=255)                                                      ttcGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCgg                                                                                                                                                                                               >  1:137844/1‑251 (MQ=255)                                                          aCCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACa                                                                                                                                                                                           <  1:24071/251‑1 (MQ=255)                                                                 gCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTAcc                                                                                                                                                                                     <  1:151161/250‑1 (MQ=255)                                                                 gCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTAcc                                                                                                                                                                                     <  1:295736/250‑1 (MQ=255)                                                                        gAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCaaa                                                                                                                                                                               >  1:79774/1‑249 (MQ=255)                                                                             ccAGCGGAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCGTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGA‑gg                                                                                                                                                                       <  2:331966/251‑1 (MQ=255)                                                                                   tAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGGAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGGAGTTG‑CTTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGA‑GGTAgggg                                                                                                                                                                 >  2:15956/1‑250 (MQ=255)                                                                                    caaGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAaggga‑gggagggcc                                                                                                                                                                >  2:65009/2‑249 (MQ=255)                                                                                                  tGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGA‑GGTAGGGACTTAAc                                                                                                                                                           <  1:257465/242‑1 (MQ=255)                                                                                                  tGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGAGGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTAGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTACGCGCCCAACGTACGTTTGTAATAGGCAATCGCGTCGGCACAGTTACCGGCAAAAGGGA‑GGGAGGGACTTAAc                                                                                                                                                           >  2:257465/1‑242 (MQ=255)                                                                                                             gACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAACGAGA‑GGTAGGGACTTAACGGCATGAGGGTGACCTCAGt                                                                                                                                       >  2:271536/1‑251 (MQ=255)                                                                                                                     ggcagCGCTCATCATGATGTCGCTTCCGGCAATGCGCGCAATGGCGTGGGCGATGGTGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGTGCTGTCCGGCGCTGAGTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGA‑TGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGaa                                                                                                                               <  1:26487/251‑1 (MQ=255)                                                                                                                        atCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGGAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCACCGTACGTTGATAATAGGCAATCGCGTCGGACCAGTTACCGGCAAA‑GAGAAGGTAGGGACTTAAAGGCATGAAGGTGACCCCGGTTAAGAGaaacc                                                                                                                            >  2:133761/1‑248 (MQ=255)                                                                                                                        atCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGA‑GGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGcc                                                                                                                            >  1:264501/1‑251 (MQ=255)                                                                                                                           gCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGA‑GGTAGGGACTTAACGGCATGATGGGGACCTCAGTTAAGAGAAGCCAGt                                                                                                                         >  1:108859/1‑251 (MQ=255)                                                                                                                                             tCCGTCAATGCGCACATAGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGTCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTctg                                                                                                                                                                                               <  2:112487/164‑3 (MQ=255)                                                                                                                                             tCCGGCAATGCTCTCATGTGCGTGGGCGATGGCTGTATCGGGAAATTGCATTCCGGAAGTGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGA‑GGTAGTGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAAt                                                                                                        <  2:349463/250‑1 (MQ=255)                                                                                                                                             tCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATATTGGGATTTCGCCGAAGTTG‑AATTTATAGAGCAGTTCCGCGCCCACCGGACGTTGATAATAGGCAATCGCGTCgg                                                                                                                                                                                               >  1:112487/1‑164 (MQ=255)                                                                                                                                                   aaTGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGA‑GGTAGGGACTTAACGGCATGATTGTGACCTCAGTTACGAGAAGCCAGTTAAGGGTAGGTCGAAATTTATAc                                                                                                  >  1:279428/1‑250 (MQ=255)                                                                                                                                                      gcgcACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGA‑GGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGa                                                                                               <  1:15956/250‑1 (MQ=255)                                                                                                                                                                   tGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCTCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGA‑GGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTg                                                                                 >  1:198710/1‑251 (MQ=255)                                                                                                                                                                        gATGGCGGTATCGGGGAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGA‑GGTAGGGACTTAAAGGCat                                                                                                                                                      >  2:282827/1‑177 (MQ=255)                                                                                                                                                                        gATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGA‑GGTAGGGACTTAACGGCat                                                                                                                                                      <  1:282827/177‑1 (MQ=255)                                                                                                                                                                                           tGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGA‑GGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATc                                                          <  1:304997/250‑1 (MQ=255)                                                                                                                                                                                                              tctcGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGc                                                                                                                                                                                  <  2:119442/112‑1 (MQ=255)                                                                                                                                                                                                              tctcGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGc                                                                                                                                                                                  >  1:119442/1‑112 (MQ=255)                                                                                                                                                                                                                   gcgcTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAAAGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGA‑GGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGGCGGCACTGTTGCCGGATg                                 <  2:297361/251‑1 (MQ=255)                                                                                                                                                                                                                            tGCGCTGATTTTGGCATTTCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGAGTCGGAACAGTTACCGGCAAAAGAGA‑GGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAAATTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATgcggagaaa                         >  1:45378/1‑245 (MQ=255)                                                                                                                                                                                                                                             ttCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGA‑GGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc       >  1:52827/1‑251 (MQ=255)                                                                                                                                                                                                                                              tCGCCGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTAGGAACAGTTACCGGCAAAAGAGA‑GGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc       <  1:176154/250‑1 (MQ=255)                                                                                                                                                                                                                                                  cGAAGTTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGA‑GGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAgg  >  1:110877/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                        GCCATTTCGATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTG‑ATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGA‑GGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGG  >  NC_000913/4325398‑4325889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑