Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,585,480	G→A	Q428* (CAA→TAA)	hsdR ←	type I restriction enzyme EcoKI endonuclease subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,585,480	0	G	A	100.0%	131.5 / NA	40	Q428* (CAA→TAA)	hsdR	type I restriction enzyme EcoKI endonuclease subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/19);  total (21/19)

GGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCAT  >  NC_000913/4585255‑4585704                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                 gggcgacgaGGAAGCGAATGCGTTTAAAACGCTGGTACTGGATCAGGCGGAACATCATGGCGATTTGCGTTCGGGTTTTACCGGTACCGGTCGCTAACGCCAGCAGGATATCTTGTTGCCCCTTGAAGATTGCCTTTTCACCCGCGCGTACGTCATCTTCCTGATAATAACGCAGTCGCAGCTCGCTCATGCCAGGGTTATCGGCAAATCACTGATTCGGTTTTTACGGTTCGCTGCCGAGCATTTCcagc                                                                                                                                                                                                         <  2:60417/248‑1 (MQ=255)  gTCGAAAAGGAAGAGAATGCGTTTAAAACGCGGGGACTGGTTCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGGTATCGGCAAACCACTGATCCTGTTTTTACGGTTCGCTGCCGAGCATTTCcagca                                                                                                                                                                                                        <  2:242586/251‑1 (MQ=255)            aaGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACAGCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTACCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTGTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCgg                                                                                                                                                                                              <  1:200655/251‑1 (MQ=255)                      gTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCAc                                                                                                                                                                                    <  1:67561/251‑1 (MQ=255)                               gCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTa                                                                                                                                                                             >  1:215448/1‑249 (MQ=255)                                     aCTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTc                                                                                                                                                                     <  1:194674/251‑1 (MQ=255)                                                        catGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcac                                                                                                                                                  >  1:169975/1‑251 (MQ=255)                                                         atGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATcacg                                                                                                                                                 >  1:322075/1‑251 (MQ=255)                                                                                accgggaccgggCGCCATCGCCAGCAGGAGCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGc                                                                                                                          <  2:169975/251‑1 (MQ=255)                                                                                 ccggtaccggtCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTAGCTCATGCAAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTAGCTGCCGAGCATTTCAAGCAGCTCTTCCGGGCGGTGCCAATCGGGTACGGCTTTCGACGTATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCt                                                                                                                         >  1:205871/1‑251 (MQ=255)                                                                                      atcggtCGCCATCGCCAGCAGGATCTCTTGTGGCTCGTTGACGATTGCCTTTTCAACCGCCAGGCCGGCACCTTCAAGATAATAACGAAGGCCCAGCTTGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGCTTTTACGGTACGCTGCCGAGCATTTCCAGCAGCTCATCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACTTTTTTCGGGTATCACCCACGTCGTGATACCAGATGCCGGtttgg                                                                                                                     <  2:80138/248‑3 (MQ=255)                                                                                         ggtCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCCTTTCCAGCAGCTCTTCCGGGCGGTGTCACTCGGGAACGGCTTTCGACATAATGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCt                                                                                                                 >  2:302032/1‑251 (MQ=255)                                                                                             gccatcgccaGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGCCGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCCCGCACGTCTCGATACCAGATGCCGCTTTTGGttt                                                                                                                 >  2:296692/1‑245 (MQ=255)                                                                                             gccatcgccaGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCt                                                                                                                 <  1:296692/247‑1 (MQ=255)                                                                                             gccatcgccaGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGGCTTCAt                                                                                                             >  1:161023/1‑251 (MQ=255)                                                                                                  cgccaGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCAttgtt                                                                                                         <  1:80600/250‑1 (MQ=255)                                                                                                     cagcagGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTgcgc                                                                                                     >  1:313008/1‑251 (MQ=255)                                                                                                      agcagGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGcgcg                                                                                                    >  2:296494/1‑251 (MQ=255)                                                                                                                              aagATTGCCTTGACAACCGCGCGGACGGCAACGTCCTGATAATAACGCAGGTCCAGCTTGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGGTTGTACGGTGCGCTGCCGAGCATGTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATAGTGCGGGTATTACGCACGTCGCGATACCAGATTCCGTTTTTGGTCTTCATTGTTGCGCGGTATTCGAGCCCGTTGGTCGAGTa                                                                            <  2:215448/249‑1 (MQ=255)                                                                                                                              aCGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTa                                                                            <  1:303760/251‑1 (MQ=255)                                                                                                                              aCGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTa                                                                            <  2:313008/251‑1 (MQ=255)                                                                                                                                 aTTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGc                                                                          >  1:247694/1‑250 (MQ=255)                                                                                                                                         ttCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGc                                                                                                                                                                                                                          >  1:85228/1‑98 (MQ=255)                                                                                                                                         ttCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGc                                                                                                                                                                                                                          <  2:85228/98‑1 (MQ=255)                                                                                                                                          tCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGAt                                                                >  1:138881/1‑251 (MQ=255)                                                                                                                                           cAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGAtt                                                               <  1:324025/251‑1 (MQ=255)                                                                                                                                                  gcgGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAAcc                                                        >  1:32753/1‑251 (MQ=255)                                                                                                                                                      aCGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGTTTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAATGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCgttg                                                    >  1:257121/1‑251 (MQ=255)                                                                                                                                                        ggCATCTTCCTGATAATTACGCAGGACCAGCTCGCTCATGCCAGGGTTATCGGCAAACCAAAGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCgttgtt                                                  <  1:60846/251‑1 (MQ=255)                                                                                                                                                            tCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGcc                                              >  1:248505/1‑251 (MQ=255)                                                                                                                                                                   gATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGgt                                        <  1:112579/250‑1 (MQ=255)                                                                                                                                                                    ataataACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGgtgt                                      >  1:74814/1‑251 (MQ=255)                                                                                                                                                                    ataataACCCAGGCCCAGCTCGCTCATGCCAGGGGTACCGGCAAACCACTGATTCTGTTTTTACGGGTCGCTGCCGAGCATTGCCAGCAGTTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGAGACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGgtgt                                      <  2:161023/251‑1 (MQ=255)                                                                                                                                                                       acaacgtatgCCCAGCTCTCTCATGTCACTGGTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTTCCGAGCATTTCCAGCATCTCTTCCTGGCTGTGCCACTCGGGTAAGTCTTTCTTTATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCTCTTTTGGTCTTCATTGTTGCGCGGTAGTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCt                                   <  1:347698/242‑1 (MQ=255)                                                                                                                                                                            gCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGcc                                >  1:119216/1‑249 (MQ=255)                                                                                                                                                                                     gctcgctcATGCCAGGGTTATCGGCAAACCACTGATGCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCATAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCa                     <  1:22578/251‑1 (MQ=255)                                                                                                                                                                                      ctcgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCAt                    >  1:23032/1‑251 (MQ=255)                                                                                                                                                                                        cgctcATGCAAGGGTTATCGGCAAATCACTGATTCTGTTTTTACGGATCGAAGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGCAAGGCTTTCGACATATTGCGGGTCTAACGCACGTCGCGCTACCAGCTGCCCATTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTCGCAGAGGGGGATTTAACACCGTTGTTTGCCGCTGGTGCCCtccacgccgggtgtatac                  >  2:47655/1‑234 (MQ=255)                                                                                                                                                                                                ccAGGGTTATCGGCAAACCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTATTCCGGGCGGTGCCACTCGGGAAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCAc          >  2:286981/1‑251 (MQ=255)                                                                                                                                                                                                        aTCGGCAACCCACTGATTCTGTTTTTACGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATCCCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCCCTGCTTCAt  >  2:140790/1‑251 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                 GGTCGACAAGGAAGAGAATGCGTTTAAAACGCTGGGACTGGATCAGGCGGAACATCATGGCGATTGCCGTACGGGTTTTACCGGTACCGGTCGCCATCGCCAGCAGGATCTCTTGTTGCCCCTTGACGATTGCCTTTTCAACCGCGCGGACGGCATCTTCCTGATAATAACGCAGGCCCAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCAT  >  NC_000913/4585255‑4585704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑