Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,050,092 C→T S87L (TCA→TTA) 
L22L (CTC→CTT
insA4 →
insB4 →
IS1 family protein InsA
IS1 family protein InsB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,050,0920CT90.0% 23.8 / ‑6.4 21S87L (TCA→TTA) 
L22L (CTC→CTT
insA4
insB4
IS1 family protein InsA
IS1 family protein InsB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (10/8);  minor base A (2/0);  total (12/9)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGCAACAGCCCGCATTATGGGCGTTGGCCTCAACACGATTTTCCGCCATTTAAAAAACTCAGGCCGCAGTCGGTAACCTCGCGCATACAGCCGGGCAGTGACGTCATCGTCTGCGCGGAAATGGACGAACAGTGGGGATACGTCGGGGCTAAATCGCGCCAGCGCTGGCTGTTTTACGCGTATGACAGGCTCCGGAAGACGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGGGCGTCTTATG  >  NC_000913/1049848‑1050287
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                    
accAGCTGTCCCTCCTGTGCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCTCTGCCGGACATCAGCGCTATCCCTCCTCTCTCTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGCAACAGCCCGCATTATGGGCGTTGCCCTCAACACGATTTTCCGCCATTTAAAAAACTTAGGcc                                                                                                                                                                                                 <  1:7348/248‑1 (MQ=11)
       tcccctcctgtccccctgctgtgggggTGGTGCGGACCGGCAAAAGAACGGCCGGACATCAGCGCGATCTCTGCTCTCACTGCCGGAAAACATGGCAACTGCAGTTCACTTACTCCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGCAACAGCTCGCATTATTGGCGTTTGCCTCAACACGATCTGCCGCCATATAAAAAACTGAGGCCGCAGTCg                                                                                                                                                                                          <  2:30984/227‑1 (MQ=11)
           ctcctGTTCATCTACTAACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCAATGACGTAAAACATGGCAACTGCAGTTCGCTTACACCGCTTCTCAACCCGGTAAGCAACAGAAAATCATTGTTATGGCCATGAATGGCGGTGGATGCCGGGCAACAGCCCGCATAATGGGCGTTTGCCTCCACACGATTTTACGCAATTTAATAAACTAGGGCCGCAGTCggaaa                                                                                                                                                                                      >  2:301220/1‑247 (MQ=11)
                             cGGGTTGGTGCGTAACGGCAAAAACGCTGCCGGACATCAGCGATATCTCTACTCTCACTGCCGTAAAACATGACCACTGCAGTTAACTTACACCGCTTCTCAACCCGGTACGCACCAGACAATCATTGCTATGGCAATGCACGGCGTTGGATTCCAGGAAACAGCACGCATTATGGGCGATGGACTCAACACGATTTTCCTACATTTAAACACCTTGGGCCGCAGTCGACAACCTCGCGCATTCAGCCg                                                                                                                                                                     >  2:139085/1‑249 (MQ=2)
                                                  aaGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGCAACAGCCCGCATTATGGGCGTTGGCCTCAACACGATTTTCCGCCATTTAAAAAACTTAGGCCGCAGTCGGTAACCTCGCGCATACAGCCGGGCAGTGACGTCATCGTCTgcgc                                                                                                                                              >  1:263844/1‑251 (MQ=14)
                                                     cACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGACATGAATGGCGTTGGATGCCGGGCAACAGCCCGCATTATGGGCGTTGGCCTCAACACGATTTTCCGCCATTTAAAAAACTTAGGCCGCAGTCGGTAACCTCGCGCATACAGCCGGGCAGTGACGTCATCGTCTGCGCgg                                                                                                                                            >  1:336161/1‑250 (MQ=14)
                                                           cGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGCAACAGCCCGCATTATGGGCGTTGGCCTCAACACGATTTTCCGCCATTTAAAAAACTTAGGCCGCAGTCGGTAACCTCGCGCATACAGCCGGGCAGTGACGTCATCGTCTGCGCGGAAATGGa                                                                                                                                     <  1:71710/251‑1 (MQ=14)
                                                            ggACATCCGCGATATCACTGCTCTAACTGCCGTAAAACATGGCAACTGCAGTTAACTTACACCGCTTCTCAACACGGTACGCACCAGAAAAACATTGATATGGCCATGAATGGCGTTGGATGCCGGGCAACAGACCGCATTATGGGCGTTGGCCTCAACACGATTTTCCGCCATTTAAAAAACTAAGGCCGCAGTCGGTCACCGCGCGCATACAGCCGGGCAGTGACGTCATCGACTGCGCGGacgtggtc                                                                                                                                    >  2:168604/1‑244 (MQ=11)
                                                                          tctcTGCTCTCACTGCCGTAAAACATGGCAACTGACGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGCAACAGCCCGCATTATGGGCGTTGGCCTCAACACGATTTTCCGCCATTTAAAAAACTTAGGCCGCAGTCGGTAACCTCGCGCATACAGCCGGGCAGTGACGTCATCGTCTGCGCGGAAATGGACGAACAGTGGGGAt                                                                                                                       >  1:92483/1‑250 (MQ=14)
                                                                                                              gttcacctgctccGCTTCACAACCCGGTACTCCACAGAAAAGCAGTGATATGGCCATGAATGGCGTTGGATGCCGGGCAACAGCCCGCATTATGGGCGTTGGCCTCAACACGATTTGCCGCCATTTAAAAAACTTAGGCCGCAGTCGGTAACCTCGCGTATACAGCCGGGTAGTGACGTCAGCGTCTGCGCGGAAATGGACGAACAGTGGGGATACGTCGGGGCTAAATCGCGCCAGCGCTGGCTGttttg                                                                                  <  2:28424/240‑2 (MQ=11)
                                                                                                                   cTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGCAACAGCCCGCATTATGGGCGTTGGCCTCAACACGATTTTCCGCCATTTAAAAAACTTAGGCCGCAGTCGGTAACCTCGCGCATACAGCCGGGCAGTGACGTCATCGTCTGCGCGGCAATGGACGAACAGTGGGGATACGTCGGGGCTAAATCGCGCCAGCGCTGGCTGTTTTACGCGt                                                                             >  1:236792/1‑251 (MQ=14)
                                                                                                                     tACACCGCTTCTCAACCCGGTACGCACCAGAAACTCATTGATATGGCCATGACTGGCGTTGGAAGACGTGCAACAGCCCGCATTATGGGCGTTGGACTCAACACGATTTTCCGCCATTTAAAAACCTTAGGCCGCAGTCGGTAACCTCGCGCATACAGCAGGGCAGTGCCGTCATCGTCTGCGCGGAAATGGACGCAAAGTGGGGATACGTCGGGGCTAAATCGCGCCAGCGCTGGCTGtttttagcgaat                                                                           >  2:19625/1‑243 (MQ=11)
                                                                                                                              tcgcAACCCGGTACACACCAGAAAATCATGGATATGGTCATGAATGGCGTTGGATGCCGGGAAACAGCCCGGATTATGGGCGTTGGCCTCAACACGATTTTCCGCCATTTAAAAAACTTAGGCCGCAGTCGGTCACCTCGCGCATACAGCCGGGCAGTGACGTCATCGTCTGCGCGGAAAGGGACGAACAGTGGGGATACGTCGGGGCTAAATCGCGCCAGAGCTGGCTGTTATACGCGTATGACAGGCt                                                                   <  2:2989/247‑1 (MQ=11)
                                                                                                                                     ccGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGCAACAGCCCGCATTATGGGCGTTGGCCTCAACACGATTTTCCGCCATTTAAAAAACTTAGGCCGCAGTCGGTAACCTCGCGCATACAGCCGGGCAGTGACGTCATCGTCTGCGCGGAAATGGACGAACAGTGGGGATACGTCGGGGCTAAATCGCGCCAGCGCTGGCTGTTTTACGCGTATGACAGGCTCCGGAAGa                                                           <  1:4989/251‑1 (MQ=14)
                                                                                                                                      cGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGCAACAGCCCGCATTATGGGCGTTGGCCTCAACACGATTTTCCGCCATTTAAAAAACTTAGGCCGCAGTCGGTAACCTCGCGCATACAGCCGGGCAGTGACGTCATCGTCTGCGCGGAAATGGACGAACAGTGGGGATACGTCGGGGCTAAATCGCGCCAGCGCTGGCTGTTTTACGCGTATGACAGGCTCCGGAAGAc                                                          >  1:281525/1‑251 (MQ=14)
                                                                                                                                          aCGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGAGGCCGGGCAACAGCCCGAATTATGGGCGTTGGCCTCATCACGATTTTCCGCCATTTAAAAAACTTAGGCCGCAGTCGGTAACCGCGCGCATACAGCCGGGCAGTGACGTCATCGTCTGCGCGGAAATGGACGAACAGTGTGGATACGTCGGGGCTAAATCGCGCCAGCTCTGGCTGTTTTACGCGTATGACAGGCTCCGGAAGACGgtt                                                      <  2:93927/251‑1 (MQ=11)
                                                                                                                                                                 ggCCATGAATGGCGTTGGATGCCGGGCAACAGCCCTCATTATGGGCGTTGGCCTCAACACGATTTTCCGCCATTTAAAAACCTTAGGCCGCAGTCGGTAACCTCGCGCATACAGCCGGGCAGTGACGTCATCGTCTGCGCGGAAATGGACGAACAGTGGGGATACGTCGGGGCTAAATCGCGCCAGCGCTGGCTGTTTTACGCGTATGACAGGCTCCGGAAGACGGTTGTTGCGCACGTATTCGGTGAACg                               <  2:92483/251‑1 (MQ=14)
                                                                                                                                                                   ccATGAATGGCGTTGGATGCCGGGCAACAGCCCGCATTATGGGCGTTGGCCTCAACACGATTTTCCGCCATTTAAACAACTTAGGCCGCAGTCGGTAACCTCGCGCATACAGCCGGGCAGTGACGTCATCGTCTGCGCGTAAATGTACGCACAGTGGGGATACGTCGGGGCTAAATCGCGCCAGCGCTGGCTGTTTTACGCGAATGACAGGCTCCGACAGACGGTTGTTGGCCACGTATTCGGTGAACGcg                             >  2:251113/1‑250 (MQ=11)
                                                                                                                                                                        aaTGTCGTTGGATGCCGGGCAACAGCCCGCATTATGGGCGTTGGCCTCAACACGATTTTCCGCAATTTAAAAAACTTAGGCCGCAGTCGGTAACCGCGCGCATACAGCCGGGCAGTGACGTCATCGTCTGCGCGGAAATGGACGAACAGTGGGGATACGTCGGGGCTAAATCGCGCTAGCGCTGGCTGTTTTACGCGTATGACAGGCTCCGGAAGACGTTTGGTGCGCACGTATTCTGTGAACGCACTAtt                        <  1:257233/251‑2 (MQ=11)
                                                                                                                                                                                          gCAACAGCCCGCATTATGGGCGTTGGCCTCAACACGATTTTCCGCCATTTAAAAAACTTAGGCCGCAGTCGGTAACCTCGCGCATACCGCCGGGCAGTGACGTCATCGTCTGCGCGGAAATGGACGAACAGTGGGGATACGTCGGGGCTAAATCGCGCCAGCGCTGGCTGTTTTACGCGTATGACAGGCTCCGGACGCAGGTTTGTGCGCACGTATTCGGGGAACGCACTATGGCCACGCATgggggtatt      >  2:18605/1‑246 (MQ=11)
                                                                                                                                                                                             aCAGCACGCAATATGGGCGTTGGCCTCAACACGATTTTACGCCATTTAAAAAACTTAGGCCGCAGACGGTAACCTCGCGCATACAGCCGGGCAGTGACGTCATCGTCTGCGCGGAAATGGACGAACAGTGGGGAAACATCGGGGCTAAATCGCGGCAGAGCTGTCTGTTTTACGCGTATAACAGGCTCCGGAAGAATGTTGTTGCGACCGTATTCGGGTAACGCCATATGGCGACGATgggggccctttat  >  2:62864/1‑242 (MQ=11)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                    
ATCAGCTGTCCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACTGCCGGACATCAGCGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAACTGCAGTTCACTTACACCGCTTCTCAACCCGGTACGCACCAGAAAATCATTGATATGGCCATGAATGGCGTTGGATGCCGGGCAACAGCCCGCATTATGGGCGTTGGCCTCAACACGATTTTCCGCCATTTAAAAAACTCAGGCCGCAGTCGGTAACCTCGCGCATACAGCCGGGCAGTGACGTCATCGTCTGCGCGGAAATGGACGAACAGTGGGGATACGTCGGGGCTAAATCGCGCCAGCGCTGGCTGTTTTACGCGTATGACAGGCTCCGGAAGACGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGGGCGTCTTATG  >  NC_000913/1049848‑1050287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: