Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,086,300	A→C	E95D (GAA→GAC)	fdhD →	sulfurtransferase for molybdenum cofactor sulfuration

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,086,300	0	A	C	100.0%	101.2 / NA	31	E95D (GAA→GAC)	fdhD	sulfurtransferase for molybdenum cofactor sulfuration
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (16/15);  total (16/15)

TCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCGCTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTACGGGCTGTGGCGTATGCGGCGTGGAGCAACTTAATGACATCGGAAAACCGGTGCAGCCGCTACCGTTCACCCAGACGTTTGATCTCAACAAACTGGATGATGCATTACGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGA  >  NC_000913/4086072‑4086541                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  tCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCGCTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGGTTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAgcc                                                                                                                                                                                                                             >  1:9322/1‑251 (MQ=255)                     gTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCGCTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCCTGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCCGCCGCCGCTTTATGGGGTTGa                                                                                                                                                                                                           >  2:131255/1‑249 (MQ=255)                           atTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCGCTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGTATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAgcgc                                                                                                                                                                                                   >  2:264387/1‑251 (MQ=255)                             tttcaaccCCCACGGTTAAAGTAGTTCACGGAAGAAGTTCCCGTTGCGCTGTTCTACAACGTCATTTCGCATGTGGTGATGATGGCTTCGCCCAAAGACCTTGAGTACTTTGCGCTCGTTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCTTCATGTATGTTGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAgcgac                                                                                                                                                                                                  <  2:9426/247‑3 (MQ=255)                               acatcccccACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCGCTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCgccgcc                                                                                                                                                                                               <  2:9322/245‑1 (MQ=255)                                caacaCCCCCGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCGCTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCTATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTc                                                                                                                                                                                                                                  <  2:130633/215‑1 (MQ=255)                                caacaCCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCGCTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTc                                                                                                                                                                                                                                  >  1:130633/1‑215 (MQ=255)                                                   gggTCGCGGAAGAAGTTCCCTTTGCGCTGGTCTACAACGGCATTTCGCATGTGGTGATTATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTACg                                                                                                                                                                           <  2:173302/250‑1 (MQ=255)                                                                          gcgcTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGGCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTACGGGCTGTGGCGTATGCGGCGTgg                                                                                                                                                     <  1:298950/250‑1 (MQ=255)                                                                                       aCGGCATTTCGCATGTGGTGATGCTGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTACGGGCTGTGGCGTATGCGGCGTGGAGCAACTTAATGAc                                                                                                                                       >  1:271153/1‑251 (MQ=255)                                                                                        cGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGGGTACTTTGCGCTCGTTTTTTCGCTTTCCGAGTGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGTATTTCGTTCCTTCCTGTAATTGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGtt                                                                                                                                                                                                             <  2:403/180‑1 (MQ=255)                                                                                        cGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGtt                                                                                                                                                                                                             >  1:403/1‑180 (MQ=255)                                                                                            aTTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGTGACGTACGGGCTGTGGCGTATGCGGCGTGGAGCAACTTAATGACATCgg                                                                                                                                  <  1:310123/251‑1 (MQ=255)                                                                                                 gCATGTGGTGATGTTGGCGTCGCCCAAAGACCTTGAGTACGTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATGACCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTACGGGCTGTGGCGTATGCGGCGTGGAGCAACTTAATGACATCGGAAAAc                                                                                                                             <  2:80289/251‑1 (MQ=255)                                                                                                 gCATGTGGTGATGATGGCGTCGCCCAAAGACCTTTAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTACGGGCTGTGGCGTATGCGGCGTGGGGCAACTTAATGACATCGGAAAAc                                                                                                                             >  1:40335/1‑251 (MQ=255)                                                                                                           atgatgGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGCTATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTACGGGCTGTGGCGTATGCGGCGTGGAGCAACTTAATGACATCGGAAAACCGGTGCAGcc                                                                                                                   >  1:32349/1‑251 (MQ=255)                                                                                                            tgatgGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTACGGGCTGTGGCGGATGCGGCGTGGAGCAACTTAATGACATCGGAAAACCGGTGCAGCCg                                                                                                                  >  2:74876/1‑251 (MQ=255)                                                                                                             gatggagtatcacAAAGACCTTGAGTAATTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCAGCGCGATATCTTCGGAATGCATGTCGTTCCTTCCTGTAATGGTCTTGACGGACCAATTGAGCTTTCCCGCCGCCGCTTTATGGGGATGAAGGATCGCCGCAGGGCGCAGGCGGGCCGTACTGGCTGAGGCGAAAGCGGCGTGGCGCAACTTCATGACATCGGCAAACAGAGGCAGCCGc                                                                                                                 >  2:292987/13‑251 (MQ=255)                                                                                                              atgGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTACGGGCTGTGGCGTATGCGGCGTGGAGCAACTTAATGACATCGGAAAACCGGTGCAGCCGCt                                                                                                                <  1:47166/251‑1 (MQ=255)                                                                                                                    gcGCCCAATGACCTAGAGTATTTTGCGCTCGTTTTGTCGCTTTCCGAGGGTATTATCGAAAGTCCGGGCGATATCTTCGGCATGGATGTTGTTCTTTCCTGAAATGGTCGTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGGGCGCCGTCGGGCGCTGGCGGGACGTACTGGCTGTGGCGTATGCGGCGTGGAGCAACTTAATGACATCGGAAACCCGGTTCAGCCGCTACCGtt                                                                                                          <  2:23792/250‑1 (MQ=255)                                                                                                                                cTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTACGGGCTGTGGCGTATGCGGCGTGGAGCAACTTAATGACATCGGAAAACCGGTGCAGCCGCTACCGTTCACCCAGACGtt                                                                                              >  1:135946/1‑251 (MQ=255)                                                                                                                                             cgcTTGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTACGGGCTGTGGCGTATGCGGCGTGGAGCAACTTAATGACATCGGAAAACCGGTGCAGCCGCTACCGTTCACCCAGACGTTTGATCTCAACaaa                                                                                 <  1:238463/251‑1 (MQ=255)                                                                                                                                                                    gggATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTACGGGCTGTGGCGTATGCGGCGTGGAGCAACTTAAGGACATCGGAAAACCGGTGCAGCCGCTACCGTTCACCCAGACGTTTGATCTCAACAAACTGGATGTTGCATTACGTCctct                                                          >  2:252138/1‑251 (MQ=255)                                                                                                                                                                      gATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGCCGTACGGGCTGTGGCGTCTGCGGCGTGGAGCAACTTAATGACATCGGAAAACCGGAGCAGCCGCGACCGTTCACCCCGACGTTTGATCTCACCAAACTGGATGATGAATTACGTCAtcac                                                         >  2:335971/1‑248 (MQ=255)                                                                                                                                                                              aaaGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTACGGGCTGTGGCGTATGCGGCGTGGAGCAACTTAATGACATCGGAAAACCGGTGCAGCCGCTACCGTTCACCCAGACGTTTGATCTCAACAAACTGGATGATGCATTACGTCATCTCAACGAtttt                                                <  1:264387/251‑1 (MQ=255)                                                                                                                                                                               caGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTACGGGCTGTGGCGTATGGGGCGGGGAGCAACTTAATGACATCGGAAAACCGGTGCAGCCGCTACCGTTCACCCAGCCGTTTGATCTCAACAAACTGGATGATGCATTACGTCCTCTAAACGATTTTc                                               >  2:95296/2‑251 (MQ=255)                                                                                                                                                                               aaGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTACGGGCTGTGGCGTATGCGGCGTGGAGCAACTTAATGACATCGGAAAACCGGTGCAGCCGCTACCGTTCACCCAGACGTTTGATCTCAACAAACTGGATGATGCATTACGTCATCTCAACGATTTTc                                               <  1:74876/251‑1 (MQ=255)                                                                                                                                                                                         gATATCTTCGGCATGGATGTCGTTACTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTACGGGCTGTGGCGTATGCGGCGTGGAGCAACTTAATGACATCGGAAAACCGGTGCAGCCGCTACCGTTCACCCAGACGTTTGATCTCAACAAACTGGATGATGCATTACGTCATCTCAACGCTTTTCAGCCAGTggg                                     >  1:83162/1‑251 (MQ=255)                                                                                                                                                                                            atCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTACGGGCTGTGGCGTATGCGGCGTGGAGCAACTTAATGACATCGGAAAACCGGTGCAGCCGCTACCGTTCACCCAGACGTTTGATCTCAACAAACTGGATGATGCATTACGTCATCTCAACGATTTTCAGCCAGTGGGGc                                   >  1:150958/1‑250 (MQ=255)                                                                                                                                                                                                tCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGACGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTACGGGCTGTGGCTTATGCGGCGTGGAGCAACTTAATGACATCGGAAAACCGGTGCAGCCGCTACCGTTCACCCAGACGTTTGATCTCAACAAACTGGATGATGCATTACGTCATCTCAACGATTTTCATCCAGTGGGGCaa                                 <  1:238007/248‑1 (MQ=255)                                                                                                                                                                                                                             ggTCTTGACGTACAAATTGAGCTTTCCAGCGGCCGCTTGATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTACGGGCTGGGGCGTATGCGGCGTGGAGCAACTTAATGACAGCGGAAAACCGGTGCAGCCGCTACCGTTCACCCAGACGTTTGATCTCAACAAACGGGAGGATGCATTACGGCATCTCAACGATTTTCAGCCAGTGGGGCAACGGACTGGTTGTACTCACGCCGCTGCCTGGa  <  1:223594/250‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  TCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCGCTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGGTCTTGAAGTACAAATTGAGCTTTCCAGCCGCCGCTTTATGGGGTTGAAGGAGCGCCGCCGGGCGCTGGCGGGACGTACGGGCTGTGGCGTATGCGGCGTGGAGCAACTTAATGACATCGGAAAACCGGTGCAGCCGCTACCGTTCACCCAGACGTTTGATCTCAACAAACTGGATGATGCATTACGTCATCTCAACGATTTTCAGCCAGTGGGGCAACTGACTGGTTGTACTCACGCCGCTGCCTGGA  >  NC_000913/4086072‑4086541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑