Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,555,731	Δ1 bp	intergenic (‑62/+95)	maeA ← / ← sra	malate dehydrogenase (oxaloacetate‑decarboxylating)/ribosome‑associated protein Sra

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,555,731	0	C	.	100.0%	94.3 / NA	23	intergenic (‑62/+95)	maeA/sra	malate dehydrogenase (oxaloacetate‑decarboxylating)/ribosome‑associated protein Sra
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (9/14);  total (9/14)

CATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTCCGGCAGTAACCCCAGCAGGTTGAAGTTACGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAAT  >  NC_000913/1555488‑1555977                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       cATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTCCGGCAGTAACCCCAGCAGGTTGAAGTTACGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTTCTTTACCGC‑AGGGCGTc                                                                                                                                                                                                                                                <  1:206922/251‑1 (MQ=255)  aTGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTCCGGCAGTAACCCCAGCAGGTTGAAGTTACGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGC‑AGGGCGTCg                                                                                                                                                                                                                                               >  1:169400/1‑251 (MQ=255)                tGTTCTTCGATGGTTTCGACCACGTCCGGCAGTAACCCCAGCAGGTTGAAGTTACGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGC‑AGGGCGTCGGCCTCAAGtata                                                                                                                                                                                                                                   <  1:208602/249‑1 (MQ=255)                       cGATGGTTTCGACCACTTCCGGCAGTAACCCCAGCAGGTTGAAGTTACGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGC‑AGGGCGTCGGCCTCAAGTATAAAGCAGAt                                                                                                                                                                                                                           <  1:68182/250‑1 (MQ=255)                                        cccGGCAGTAACCCCAGCAGGTTGAAGTTACGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGC‑AGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCAtt                                                                                                                                                                                                         <  2:96794/250‑1 (MQ=255)                                                 aaCCCCAGCAGGTTGAAGTTTCGGCGTTCGTCCATGCTGAAGGCACTGCTTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCATCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGC‑AGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCa                                                                                                                                                                                                <  1:288143/251‑1 (MQ=255)                                                     ccAGCAGGTTGAAGTTACGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGC‑AGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATgg                                                                                                                                                                                            >  1:245118/1‑251 (MQ=255)                                                        gcagGTTGAAGGTACGGAGTTCTTCCAGGCTGAAGTCACTGGCTTTATGCTACAACGGAAATTACAGTAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTGGTTTTTGGTTCCATGTCACTCACGCTTTGGTGAATATCCATCCCTGGGGGACTTTTATCTTCTTTGCTTTTCCGC‑AGGGCGTCGGCCTCAAGTATTAAGCATATAAAAACAAAAACACCATTGCGCAGGCAATGgtgt                                                                                                                                                                                         <  2:51202/251‑1 (MQ=255)                                                                                 cATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGC‑AGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGt                                                                                                                                                                <  1:185415/251‑1 (MQ=255)                                                                                                  cTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGC‑AGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTc                                                                                                                                                 >  1:136565/1‑249 (MQ=255)                                                                                                      aTTCAACAACGGAAATACCAGCAGTACAGGGCCAGCTTAAGGGATATAAAGCGAACGCTGTTTGTTTGTTTTTGGTGCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGACTTTGCTTTACCGC‑AGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGTCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACATTTCAGCggg                                                                                                                                           <  2:44595/251‑1 (MQ=255)                                                                                                                                 aGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGC‑AGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTtac                                                                                                                <  1:73151/251‑1 (MQ=255)                                                                                                                                  gggCCCGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCAATCTTTTTTGACTTTCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGA‑AGGGCGTCGGCCTCAAGTATAAAGCGAATAAAAACAAAAACACCATTACGAAGGCAATGGTGTTTAATCGTCATTGATGACTGATGGTTATGAATTAATTTTCAACGGGGCGTTTTATGCCGGTTGGTTTAtttcct                                                                                                               >  2:311540/1‑248 (MQ=255)                                                                                                                                           ctcgggATATCAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGACTTTTATCGTCTTTGCTTTACCGC‑AGGGCGTCGGCCTCAAGTTTAAAGCCGTTAAAAACAAAAACACCATTGAGCAGGCCATGGTGTTTAATCGTCATTGAGGACTGATGGGTATGGATTCCTTTTGAGCGGGGCGTTTTATGCGAGTTGGGTTATTTACTACGCTGGAt                                                                                                      >  2:62122/4‑251 (MQ=255)                                                                                                                                            aaGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGC‑AGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTta                                                                                                                 >  2:145209/1‑239 (MQ=255)                                                                                                                                            aaGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGC‑AGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTta                                                                                                                 <  1:145209/239‑1 (MQ=255)                                                                                                                                                                   ttttttGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGC‑AGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTAtttt                                                                               <  2:136565/250‑1 (MQ=255)                                                                                                                                                                       ttgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGC‑AGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCt                                                                          >  2:212556/1‑251 (MQ=255)                                                                                                                                                                                             cactcTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGC‑AGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtccag                                                    <  1:212556/251‑1 (MQ=255)                                                                                                                                                                                                                      gggtggCTTTTATCGTCTTTGCCTTTCCGC‑ATGGCGTCGGTCTCAAGTATAATTCAGATAAAAACAAAAACACCATTGCGCAAGAAATGGTGTTTAATCGTCTTTGAGGACGGATGGTTATGAAGTTCTTTTCAGCGGGGCGTTTTCTGCCGGTTGGCTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCTTTGGTTAGAAATTTTATGGTCCAGTCCAAAAATATgccctgcttgtcgg                           <  2:333880/247‑14 (MQ=255)                                                                                                                                                                                                                       gggggCTTTTATCGGCTTTGCTTTACCGC‑AGGGCGGCTGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGt                          <  1:161763/251‑1 (MQ=255)                                                                                                                                                                                                                           gCTTTTATCGTCTTTGCTTTACCGC‑AGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCg                       >  1:170891/1‑250 (MQ=255)                                                                                                                                                                                                                                       tttGCTTTACCGC‑AGGGAGTCGGCCTCAAGTATAAAGCAGTTAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGCTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACGATTTTGCGCTGGTTAGAATTTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGTTTTCATTTTAAc          >  2:42787/1‑251 (MQ=255)                                                                                                                                                                                                                                                acagcAGGGCGTCGGCCTCAAGAATACCCCAGATCAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTAATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTGCTGCCGGTTGGGTTATTTACTACGCTGTATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGCAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTCActcctaact  >  2:292263/5‑247 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       CATGCTCGTTCCGCTTGTTCTTCGATGGTTTCGACCACTTCCGGCAGTAACCCCAGCAGGTTGAAGTTACGGCGTTCTTCCATGCTGAAGGCACTGCCTTTATTCAACAACGGAAATTCCAGCAGTACAGGGCCAGCGTAAGGGATATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAAGAATATGACGTGCCTGACGGTTCGATTTCATTTTAACTCCTCAAT  >  NC_000913/1555488‑1555977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑