Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,447,871 +C coding (279/372 nt) rplN ← 50S ribosomal subunit protein L14

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,447,8711.C86.1% 102.5 / 8.3 37coding (279/372 nt)rplN50S ribosomal subunit protein L14
Reads supporting (aligned to +/- strand):  ref base . (3/2);  new base C (15/16);  total (19/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

CGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTACCGCGTTTACCTTTATCTTTACCGGTTAACACGATAACTTCGTCATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGC‑TGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTC  >  NC_000913/3447624‑3448116
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     
cGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTACCGAGTTTACCTTTATCTTTACCGGTTAACACGATAACTTCGTCATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAACTCACGAGTTACCGGCCCAAAAATACGCGTACCGAAA‑Ggcctg                                                                                                                                                                                                                                                     >  2:40992/1‑248 (MQ=255)
        gATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTACCGCGTTTACCTTTATCTTTACCGGTTAACACGATAACTTCGTCATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCtgt                                                                                                                                                                                                                                             >  1:108965/1‑251 (MQ=255)
        gATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTACCGCGTTTACCTTTATCTTTACCGGTTAACACGATAACTTCGTCATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCtgt                                                                                                                                                                                                                                             <  2:108965/251‑1 (MQ=255)
         aTGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTACCGCGTTTACCTTTATCTTTACCGGTTAACACGATAACTTCGTCATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCtgtt                                                                                                                                                                                                                                            <  1:164143/251‑1 (MQ=255)
                                   caacaaTGACCTTGCCGGAAGACAGGACATTCTTAACTTTACCGCGTTTACCTTTATCTTTACCGGTTAACACGATAACTTCGTCATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGATGAACACAAGCATTAc                                                                                                                                                                                                                  >  1:124853/1‑251 (MQ=255)
                                     acaaTGACCTTGCCGGAAGACAGGACATTCCTAACTTTACCGCGTTTACCTTTATCTTTACCGGTTAACACGATAACTTCGTCATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGAGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGC‑TGCTCGCTGTTGTTGTTCAGAAGAACACAAGCCTTACCAt                                                                                                                                                                                                               >  2:74441/1‑251 (MQ=255)
                                          caCCTTGCCGGATGACAGGACATTCTTAACTTTACCGCTTTTACCGTTACCTTTACCGGTTAACACGATAACTTCGTCATCACGACGGATTTTCGCTGCCATTATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGTCCAGAAGAACACAAGCATTACCATCGaa                                                                                                                                                                                                           <  2:260345/250‑1 (MQ=255)
                                                         cAGGACATTCTTAACTTTACCGCGTTTACCTTTATCTTTACCGGTTAACACGATAACTTCGTCATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAAcc                                                                                                                                                                                            <  1:257855/251‑1 (MQ=255)
                                                               aTTCTTAACTTTACCGCGTTTACCTTTATCTTTACCGGTTAACACGATAACTTCGTCATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCgg                                                                                                                                                                                      <  1:288891/251‑1 (MQ=255)
                                                                           aCCGCGTTTACCTTTATCTTTACCGGTTAACACGATAACTTCGTCATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACAGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACAcc                                                                                                                                                                          >  1:293869/1‑251 (MQ=255)
                                                                                gTTTACCTTTATCTTTACCGGTTAACACGATAACTTCGTCATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCtgttgttg                                                                                                                                                                                                                                        >  1:138155/1‑184 (MQ=255)
                                                                                gTTTACCTTTATCTTTACCGGTTAACACGATAACTTCGTCATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCGGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTGTCCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCtgttgttg                                                                                                                                                                                                                                        <  2:138155/184‑1 (MQ=255)
                                                                                  ttACCTTTATCTTTACCGGTTAACACGATAACTTCGTCATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCcttctt                                                                                                                                                                    <  1:52133/250‑1 (MQ=255)
                                                                                   tACCTTTATCTTTACCGGTTAACAAGATAACTTCGTCATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTgg                                                                                                                                                                  >  1:287202/1‑251 (MQ=255)
                                                                                            cTTTACCGGTTAACACGATAACTTCGTCATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGcacc                                                                                                                                                          >  1:10013/1‑250 (MQ=255)
                                                                                                aCCGGTTAACACGATAACTTCGTCATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAg                                                                                                                                                                                                          <  2:272616/198‑1 (MQ=255)
                                                                                                aCCGGTTAACACGATAACTTCGTCATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAg                                                                                                                                                                                                          >  1:272616/1‑198 (MQ=255)
                                                                                                       aaCACGATAACTTCGTCATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTc                                                                                                                                              >  2:95471/1‑251 (MQ=255)
                                                                                                             aTAACTTCGTCATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGCAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCATGCTCGCTGTTGTTGTTCAGAAGAAAACAAGCATTACCATCGAAGAGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACAACTACCGCCTTCCGcacc                                                                                                                                        >  2:343239/1‑250 (MQ=255)
                                                                                                             aTAACTTCGTCATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGC‑TGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATTACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACAt                                                                                                                                       >  1:147377/1‑251 (MQ=255)
                                                                                                                        atcaCGCCGGATTTTCGATGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGTGATAATTTTAATGAACTTCTCACTACGCAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAAGCC‑TGCTCGCTGTTGTTGTTCAGCAGAACACAAGAATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGACAACCCTTCTTGGTGCGCAACACTACCGCCATCAGCAAATACCCtttttt                                                                                                                             >  2:322695/1‑251 (MQ=255)
                                                                                                                           acgacgGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGAGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGC‑TGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGAcc                                                                                                                         <  1:74441/251‑1 (MQ=255)
                                                                                                                              acgGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCtt                                                                                                                       <  1:63438/251‑1 (MQ=255)
                                                                                                                                        gCTGCCATGATTCGCTCCTTAGAGTACTTCTGGAGCCAGAGAGATAATTTTCATGAACTTCTCACTACGACGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACACACGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGCACACCCTTCTTGGTGAGACCCACTACCGCCTTCAGCACATAACCTTTTTTGACCTTACCACACgg                                                                                                              >  2:2881/1‑250 (MQ=255)
                                                                                                                                               tGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGctt                                                                                                      >  1:145270/1‑251 (MQ=255)
                                                                                                                                                gATTCGCTCCTTAGAGGTCTTCTGGTGACAGAGAGATAATTTGCAAGAACTTCTCACTACGAAGCTCACGAGTTGCCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGCTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTAACGCCTTCAGCACATCACCTTTTTTGACCTGACCGCGCGGAATTGctt                                                                                                      <  2:175471/250‑1 (MQ=255)
                                                                                                                                                  ttCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACAGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGcttctt                                                                                                   >  2:245253/1‑251 (MQ=255)
                                                                                                                                                        ccgtAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTCCCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACCCGGAATTGCTTCTTTGAt                                                                                               <  1:99010/246‑1 (MQ=255)
                                                                                                                                                        ccTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTGCCATCGAAGCGAATGACCGAACCGTCCGGGCGACGAACACCCTTCTTGGGGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGATTCTTTGATgg                                                                                             >  1:53323/1‑251 (MQ=255)
                                                                                                                                                            agagTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGAt                                                                                         <  1:40992/251‑1 (MQ=255)
                                                                                                                                                                               atagaTTATTTTCATTAACTTCTCACTACGAAGCTCACTAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACTCAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCTACTAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTCTTAGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACg                                                                      <  2:292520/249‑1 (MQ=255)
                                                                                                                                                                                  gaTAATATGCATGAACGTCTCACTACGAAGCGCACAGGTGACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGACCCGTCCGGGCGACGAACACCCCTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTTCCACGCGGAATTGCTTCTTTGAGGGTGATCTTGATGATGTCGCCTACGCCt                                                                   <  2:145270/251‑1 (MQ=255)
                                                                                                                                                                                                            gAAGCTCACGAGTTAACGGCACAAAAATACGCGTAACGATC‑GGCCTGCTCGATGTTGTTGATCAGAAGAACCCAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTATTGATGCGCACCACTACCGCCGTCACCACATCACCTTGTTTAACCTAACCACGCGGCATTGGTACTTTGATGGAGATCTAGTTGATCTCGCCTACGCCTGCGTAGcagctgttggagcccctc                                           >  2:202333/1‑232 (MQ=255)
                                                                                                                                                                                                               gCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAAc                                      <  1:2881/251‑1 (MQ=255)
                                                                                                                                                                                                                           ccGGCCCAAAAATACGCGTACCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACAt                          >  1:200758/1‑251 (MQ=255)
                                                                                                                                                                                                                             ggaccAAAAATCCGCGTACCGATA‑GGC‑TGCTCGCTGTTGTTTTGCAGAAGAACACAAGCATTACCAGCGAAGCGAAATACAGATCCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCCTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTAc                       <  2:147377/248‑1 (MQ=255)
                                                                                                                                                                                                                                            gTACCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATAAACATTACGCGACGTGCACCgg         >  1:327731/1‑251 (MQ=255)
                                                                                                                                                                                                                                              aCCGATA‑GGCCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAg       <  1:95471/251‑1 (MQ=255)
                                                                                                                                                                                                                                                    aggcc‑tgctCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTc  <  1:245253/246‑1 (MQ=255)
                                                                                                                                                                                                                                                       cc‑tgctCGCTGTTGTTGTTCAGAAGACCACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCAcc                                                                                                                                   >  1:130318/2‑118 (MQ=255)
                                                                                                                                                                                                                                                       cc‑tgctCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCACCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTTGTGCGCACCACTACCGCCTTCAGCACATCAcc                                                                                                                                   <  2:130318/117‑1 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     
CGGCTTCTGATGTTTCTTAACCAGGTTGATACCTTCAACAATGACCTTGCCGGAAGACAGGACATTCTTAACTTTACCGCGTTTACCTTTATCTTTACCGGTTAACACGATAACTTCGTCATCACGACGGATTTTCGCTGCCATGATTCGCTCCTTAGAGTACTTCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATA‑GGC‑TGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTCCGGGCGACGAACACCCTTCTTGGTGCGCACCACTACCGCCTTCAGCACATCACCTTTTTTGACCTTACCACGCGGAATTGCTTCTTTGATGGTGATCTTGATGATGTCGCCTACGCCTGCGTAGCGACGGTGCGAGCCACCCAGAACCTTGATACACATTACGCGACGTGCACCGGAGTTGTC  >  NC_000913/3447624‑3448116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑