Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,925	G→C	R77P (CGC→CCC)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,925	0	G	C	100.0%	112.6 / NA	35	R77P (CGC→CCC)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (10/25);  total (10/25)

AATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACG  >  NC_000913/4636695‑4637164                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                aaTGCGTATCGGCTTGCGGTTGTTGCTGGGCTTTTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCg                                                                                                                                                                                                                             <  2:50126/251‑1 (MQ=255)       ttgcggggtgctgttgttGCTTGGCTATTTGTGACGGGTTGCGGTGGCAGCCTGGTTCGTACTAGCTATTTTTGTAGAAGAAGTTAGACCGGGCGTGCTATGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGTCCTAACGCATGTGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGGGGc                                                                                                                                                                                                                        <  2:117547/238‑1 (MQ=255)        aTCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCAt                                                                                                                                                                                                                      <  1:127201/251‑1 (MQ=255)          cGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTa                                                                                                                                                                                                                    <  1:159636/251‑1 (MQ=255)                gccgttgttGCTGGGCGATTGTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTGGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACaaa                                                                                                                                                                                                               <  1:239424/247‑1 (MQ=255)                     tgttgCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTgcgc                                                                                                                                                                                                         >  1:147820/1‑251 (MQ=255)                            gggCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAat                                                                                                                                                                                                  >  1:95032/1‑251 (MQ=255)                                        aCTGGGGGCGGTGGCAGCCTGGTTCGTACTGGCCATATTTGTCAAAGAAGTTAAACCGGTCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAGCGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCGCTGGGGACCCAACGCATGTGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCtata                                                                                                                                                                                      <  1:323455/251‑1 (MQ=255)                                                     gcAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGAGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATACCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATAATGTCTATATGACCGATgagc                                                                                                                                                                          >  1:207572/1‑247 (MQ=255)                                                                 gTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTAtt                                                                                                                                                             <  1:66254/251‑1 (MQ=255)                                                                 ctACTGGCCATTTTTGTCCAAGAAGTTAAACCGCGCGTGCGCAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCCGAGCTCGCGCGGCCCGATTTGCCCTCTGGGGGCACAACGCCTGGGCAACTGGCGCAGGCGTTTACTCAGCTCCAACATCCCCCGTTTCGCGCCAATATCGGAGGCCTTAACAAAGTGCGCAACGAATATCATGTCTATATTAACGATGCGCAGGGCAAAGtgtt                                                                                                                                                             >  2:82442/2‑248 (MQ=255)                                                                   aCTGGCCATTTTTGTCAAATAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGTCGGAGCTGTCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCTCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTg                                                                                                                                                            <  1:220397/250‑1 (MQ=255)                                                                   aCTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGt                                                                                                                                                           >  1:348867/1‑251 (MQ=255)                                                                        ccATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATAGGACCGATGCGCAGGGCAAAGTATTGTTCGAt                                                                                                                                                      <  1:82442/251‑1 (MQ=255)                                                                             tttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCgg                                                                                                                                                  <  1:2947/250‑1 (MQ=255)                                                                                  cAAATAAGTTAAACCGGGCGTGGGAAGAGCAACGGAGGGGACGTTGATCGTAACCGCAACGTTGCTGGCGGGGCTGTCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGTCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGTGCAAAGTATTGGTCGATTCGGCAAATa                                                                                                                                            <  2:135974/251‑1 (MQ=255)                                                                                   aaaGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCGGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATaa                                                                                                                                           <  2:95032/251‑1 (MQ=255)                                                                                           tAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGtt                                                                                                                                                                                                                                           <  2:59583/147‑1 (MQ=255)                                                                                           tAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGtt                                                                                                                                                                                                                                           >  1:59583/1‑147 (MQ=255)                                                                                                 gggCGTGCGAAGAGCAACGGAGGGGACGTTGCTCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGCTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAACGAGCGCAATGAATATCATGACTAAATGGCCGAAGCGCAGGGCCAAGGATTGTTCGATTCGGAAAATagatcggaagagggg                                                                                                                              >  2:186578/1‑236 (MQ=255)                                                                                                 gggCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAAt                                                                                                                                             <  1:186578/235‑1 (MQ=255)                                                                                                   gcgggCGAAGAGCAACGGAGGGGACGGTGATCGACGACTCAACGTTGCTGGAGGGGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACGGGCGCAGGCGTTTAATCAGCTACAACCTCCCCCGTTTTGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAATATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGa                                                                                                                            <  2:98110/246‑1 (MQ=255)                                                                                                     gTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGAGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATAAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGAGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTAGGCAAATAAAGCCGTTGGACAGGatt                                                                                                                          >  1:41784/1‑250 (MQ=255)                                                                                                       gggacgaggaccgggggggACGTTGATCAACACCGCAACTTTGCGGTCGGCGCTAGCGTGTCCCGATTTGCTCGCTGGGGACCCAACGCATGGGCATCTGGCGCAGGCGTGTAATCAGCTACAACATCCCCCGTTTCGCGCCAATAGCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTAGATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAATCCGTTGGACAGGattatg                                                                                                                       <  2:135923/236‑2 (MQ=255)                                                                                                                           cGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTTCCGATTTGCTCTCCGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAATATCCCCCGTTTCGCGCCAATAGCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTgg                                                                                                   <  1:48823/251‑1 (MQ=255)                                                                                                                                        cgcaactgtGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTTGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTg                                                                                      <  1:64724/243‑1 (MQ=255)                                                                                                                                         gCAACGTTGCTGGAGGAGCTGGCGGGTCCCGATTTGCCCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGGTTCGCGACAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGTAAAGTATTGTGCGATTCGGCAAATAAAGCCGTTGGACAGGAGTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTgg                                                                                     <  2:47195/251‑1 (MQ=255)                                                                                                                                          cAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGAACCAACGCATGGGCACCTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGCCAGGATTATTCGCGCTGGAATGACGTCTGGCTTACGTTGCGTGGt                                                                                    >  1:146562/1‑251 (MQ=255)                                                                                                                                           aaCGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTc                                                                                   <  2:147820/251‑1 (MQ=255)                                                                                                                                                    ggcgtaggtGGCGCGTCCCTATTGTCTCTCTGGGGACCCAACGCATGGGCAGCGGGCGCATGCGTTTAATCAGCTTCAACATCCCCCGTTTCGCGCTAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGTCAGGAGTATTCGCTCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGt                                                                           <  2:270197/242‑1 (MQ=255)                                                                                                                                                              gcgcGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCAc                                                                <  1:233809/251‑1 (MQ=255)                                                                                                                                                                 cgTCCCGATTTGCTCTCGGGGGACCCAACGCATTGGTAACTGGCGAAGGCGTTTAATCAGCGACAACATCCCCCGTTTCGCGCCAATAGCGGTGGCATTAACAAAGTGCTCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATGCGGCAAATAAAGCCGGTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGtt                                                             <  2:245952/251‑1 (MQ=255)                                                                                                                                                                                   ggggACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCACCTACAACATCCCCCTTTTCGCCCCAATATCGGTGGCATTAACAAAGGGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGTACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGAt                                             <  1:58360/249‑1 (MQ=255)                                                                                                                                                                                                                            gCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACg  <  1:27879/251‑1 (MQ=255)                                                                                                                                                                                                                            gCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACg  >  1:163328/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                AATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACG  >  NC_000913/4636695‑4637164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑