BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	659,517	G→A	L234L (CTG→TTG)	lipA ←	lipoyl synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	659,517	0	G	A	97.1%	95.2 / NA	34	L234L (CTG→TTG)	lipA	lipoyl synthase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (17/16); minor base C (1/0); total (18/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

AATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGCTTTCATTTCGTCGAACTCATCCGGGCTAACGTAACGTTGAACCGGCAGGTGATGGCGGCTTGGCTGCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCATTGGTTTCACCCAGTCCCACCATCAGACCAGACTTGGTCGGGATTTCCGGATGCGCTTCTTTAAAGCGTTCCAGCAGCTTCAGCGACCAGTTGTAATCTGCACCAGGCCGTACCTGACGGTAAATACGCGGTACGTTTTCCAGGTTATGGTTGAACACATCTGGTGGCGTTGCAGTCAGAATATCCAGAGCACG‑ATCCATACGACCGCGGAAATCCGGCACCAGAGTTTCAATTTTGATTTGCGGGCTTTTTTCCCGAATGGC  >  NC_000913/659280‑659754

aaTCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGCTTTCATTTCGTCGAACTCATCCGGGCTAACGTAACGTTGAACCGGCAGGTGATGGCGGCTTGGCTGCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGACGCGCATTACCTAAATAATTTCTTCCTTGGGTTCACCCAGTCCCACAATCAAACCAGACTTTGTc                                                                                                                                                                                                                                   >  1:166770/1‑251 (MQ=255)

   cGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGCTTTCATTTCGTCGAACTCATCCGGGCTAACGTAACGTTGAACCGGCAGGTGATGGCGGCTTGGCTGCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCATTGGTTTCACCCAGTCCCACCATCAAACCAGACTTTGTCggg                                                                                                                                                                                                                                <  1:84538/251‑1 (MQ=255)

         ggTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGCTTTCATTTCGTCGAACTCATCCGGGCTAACGTAACGTTGAACCGGCAGGTGATGGCGGCTTGGCTGCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCATTGGTTTCACCCAGTCCCACCATCAAACCAGACTTTGTCGGGATTTc                                                                                                                                                                                                                           <  1:286453/250‑1 (MQ=255)

              gaagaGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGCTTTCATTTCGTCGAACTCATCCGGGCTAACGTAACGTTGAACCGGCAGGTGATGGCGGCTTGGCTGCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCATTGGTTTCACCCAGTCCCACCATCAAACCAGACTTTGTCGGGATTTCCGGATg                                                                                                                                                                                                                     <  1:9493/251‑1 (MQ=255)

                         aaaCGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGCTTTCATTTCGTCGAACTCATCCGGGCTAACGTAACGTTGAACCGGCAGGTGATGGCGGCTTGGCTGCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCATTGGTTTCACCCAGTCCCACCATCAAACCAGACTTTGTCGGGATTTCCGGATGCGCTTCTTTa                                                                                                                                                                                                           >  1:58545/1‑250 (MQ=255)

                          aaCGGACCGCATGCAGAATGGGTAAAGCCCATCACCAGCGCTTAGGCTTTCATTTCGTCGAACTCATCCGGGCTAACGTAACGTTGAAACGGCAGGTGATGGCGGCTTGGCTGCAAATATTGCCACAGCGTTAACATCGTCACAACATGACGTCGCCGGACGCGCATTAACTCAATAATTTCTTCATTGGTTTCACACAGTACCACCAACAAACCAGAATTTGTAGGGATTGCCTGTTGCGcttcttaat                                                                                                                                                                                                          >  2:345951/1‑247 (MQ=255)

                                   acgaactcaTGGGTAAAGCCCATCGCCAGCGCTTCGGCTTTCATTTCGTCGAACTCAGCCGTGCTAACGGAACGTGGAACCGGCAGGTGATGTCGGCTTGGCTGCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCAGTTGTTTCACCCAGTCCCACCATCAAACCAGACGTTGTCGGGATGTCCGGATGCGCTTCTTTAAAGCGTTCca                                                                                                                                                                                                 <  2:215993/243‑1 (MQ=255)

                                     ggcagcaTGGGTAAAGCCCAACGCAAGCGCTGCGGCTTTCATTTCGTCGAACTCATCCGGGCTAACGTAACGTTGAACCGGCAGGTGATGGCGGCTTGGCTGCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCATTGGTTTCCCCCAGTCCCACCATCAAACCAGCCTTTGTCGGGATTTCCGGATGCGCTTCTTTAAAGCGTTCcagca                                                                                                                                                                                              <  2:193500/250‑1 (MQ=255)

                                         gcaTGGGTAAAGCCCATCGCCAGCGCTTCGGCTTTCATTTCGTCGAACTCATCCGGGCTAACGTAACGTTGAACCGGCAGGTGATGGCGGCTTGGCTGCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCATTGGTTTCACCCAGTCCCACCATCAAACCAGACTTTGTCGGGATTTCCGGATGCGCTTCTTTAAAGCGTTCCAGCAGCtt                                                                                                                                                                                          >  1:265045/1‑251 (MQ=255)

                                          caTGGGTTAAGCCCATCGCCAGTGCTTCGGCTTTCATTTCGCCGCTCTCATCCGGGCTAACGTAACGTTGAACCGGCAGGTGAGTGCGGATTGGCTGCAAATATTGCCCCAGCGTTAACATCGGCATCCCATGACGGCGCAGGTCGCGCATTACCTCAATTAGTTCTTCATTGGTTTCACCCAGTCCCACCATCAAACCAGACTTTGTCTGGATTTCCGGATGCGCTTATTTAAAGCGTTCCAGCAGCTTc                                                                                                                                                                                         <  2:308117/251‑1 (MQ=255)

                                          caTGGGTAAAGCCCATCGCCAGCGCTTCGGCTTTCATTTCGTCGAACTCATCCGGGCTAACGTAACGTTGAACCGGCAGGTGATGGCGGCTTGGCTGCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCATTGGTTTCACCCAGTCCCACCATCAAACCAGACTTTGTCGGGATTTCCGGATGCGCTTCTTTAAAGCGTTCca                                                                                                                                                                                                 >  1:215993/1‑243 (MQ=255)

                                                   aGCCCATCGCCAGCGCTTCGGCTTTCATTTCGTCGAACTCATCCGGGCTAACGTAACGTTGAACCGGCAGGTGATGGCGGCTTGGCTGCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCATTGGTTTCACCCAGTCCCACCATCAAACCAGACTTTGTCGGGATTTCCGGATGCGCTTCTTTAAAGCGTTCCAGCAGCTTCAGCGACCAg                                                                                                                                                                                <  1:333109/251‑1 (MQ=255)

                                                                   ttCGGCTTGCATTTCGTCGAACTCATCCGGGCTCACGTAACGTTGAACCGGCAGGTGATGGCGGCTTGGCTGCAAATATTACCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCATTAGTTTAACCCAGTCCCACCCTCAAACCAGACTTTTTCGGGTTTTCCGGATGCGCTTCTTTAAAGCGGTCCAGCAGCTTCAGCAACCAGTTGTAATCTGCAcc                                                                                                                                                                  >  2:349503/1‑249 (MQ=255)

                                                                     cGGCTTTCATTTCGTAGCACTCATCCGGGCTAACGTAACGTTGAACCGGCAGGTGATGGCGGCTTGGCTGCAAATATTGCCCCAGCGGTAACATCGTCACAACATGACGGCCCAAGTCCCGCATTACCTCAAAAATTTCTTCATTGGGTTCACCCAGGCCCCCCATCCAACCAGACTTTGTCGGGATTTCCGGCTGCGCTTCTTTAAAGCGTTCCAGCAGCTTCAGCGACCCGTTGTAATCTGCACCAGGc                                                                                                                                                              >  2:117497/1‑251 (MQ=255)

                                                                        cTTTCATTTCGTCGAACTCATCCGGGCTAACGTAACGTTGAACCGGCAGGTGATGGCGGCTTGGCTGCAAATCTTGCCCCAGCGTTAACATCGTCACAACATGACGGCGCAGGTCGCGCATTACCTCCATAATTTCTTCATTGGTTTCAACCAGACCCACCATCACACCAGACTTTTTCGGGTTTTACGGATGCGCTTCTTTAAAGCGTTCCAGAAGCTTCAGCGACCAGTTGTGATATGCACCAGGCCgg                                                                                                                                                           >  2:328567/1‑250 (MQ=255)

                                                                                   tcgAACTCATCCGGGCTAACGTAACGTTGAACCGGCAGGTGATGGCGGCTTGGCTGCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCATTGGTTTCACCCAGTCCCACCATCAAACCAGACTTTGTCGGGATTTCCGGATGCGCTTCTTTAAAGCGTTCCAGCAGCTTCAGCGACCAGTTGTAATCTGCACCAGGCCGTACCTGACggg                                                                                                                                                 >  1:112621/1‑249 (MQ=255)

                                                                                           aTCCGGGCTAACGTAACGTTGAACCGGCAGGTGATGGCGGCTTGGCTGCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCATTGGTTTCACCCAGTCCCACCATCAAACCAGACTTTGTCGGGATTTCCGGATGCGCTTCTTTAAAGCGTTCCAGCAGCTTCAGCGACCAGTTGTAATCTGCACCAGGCCGTACCTGACGGTAAATAcgcg                                                                                                                                        >  1:35353/1‑251 (MQ=255)

                                                                                             ccGGGCTAACGTAACGTTGAACCGGCAGGTGATGGCGGCTTGGCTGCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCCTTACCTCAATATTTTCTTCATTGTTTTCACCCAGTCCCACCATCAAACCAGACTTTGTCGGGATTTCCGGATGCGCTTCTTTTAAGCGTTCCAGCAGATTCAGAGACCAGTTGTCATCTGCACCAGTCCGTACCTTGCGGTaacaacgcagt                                                                                                                                      >  1:264581/1‑242 (MQ=255)

                                                                                               gggCTAACGTAACGTTGAACCGGCAGGTGATGGCGGCTTGGCTGCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCATTGGTTTCACCCAGTCCCACCATCAAACCAGACTTTGTCGGGATTTCCGGATGCGctt                                                                                                                                                                                                                <  2:65658/175‑1 (MQ=255)

                                                                                               gggCTAACGTAACGTTGAACCGGCAGGTGATGGCGGCTTGGCTGCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCATTGGTTTCACCCAGTCCCACCATCAAACCAGACTTTGTCGGGATTTCCGGATGCGctt                                                                                                                                                                                                                >  1:65658/1‑175 (MQ=255)

                                                                                                   taacgtaacgTTGAACCGGCAGGTGATGGCGGCTTGGCTGCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCATTGGTTTCACCCAGTCCCACCATCAAACCAGACTTTGTCGGGATTTCCGGATGCGCTTCTTTAAAGCGTTCCAGCAGCTTCAGCGACCAGTTGTAATCTGCACCAGGCCGTACCTGACGGTAAATACGCGGTACGttt                                                                                                                                >  1:164935/1‑251 (MQ=255)

                                                                                                                 aCCGGCAGGTGATGGCGGCTTGGCTGCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCATTGGTTTCACCCAGTCCCACCATCAAACCAGACTTTGTCGGGATTTCCGGATGCGCTTCTTTAAAGCGTTCCAGCAGCTTCAGCGACCAGTTGTAATCTGCACCAGGCCGTACCTGACGGTAAATACGCGGTACGTTTTCCAGGTTATGGtt                                                                                                                  >  1:223632/1‑251 (MQ=255)

                                                                                                                       aGGTGATGGCGGCTTGGCTGCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCATTGGTTTCACCCAGTCCCACCATcaaaccag                                                                                                                                                                                                                                           >  2:182381/1‑124 (MQ=255)

                                                                                                                       aGGTGATGGCGGCTTGGCTGCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCATTGGTTTCACCCAGTCCCACCATcaaaccag                                                                                                                                                                                                                                           <  1:182381/124‑1 (MQ=255)

                                                                                                                                          gCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCATTGGTTTCACCCAGTCCCACCATCAAACCAGACTTTGTCGGGATTTCCGGATGCGCTTCTTTAAAGCGTTCCAGCAGCTTCAGCGACCAGTTGTAATCTGCACCAGGCCGTACCTGACGGTAAATACGCGGTACGTTTTCCAGGTTATGGTTGAACACATCTGGTGGCGTTGCAGTc                                                                                         <  1:211735/251‑1 (MQ=255)

                                                                                                                                                  tGCCCCAGCGTTAAAATTGTCACACCATTGCGGCTCAGGTCGCGCATTACCTTAATAATTTCTTCATGGGTTTCCCCCAGTCCCACCATCAAACCAGACTTTGTCGGGATTTCCGGATGCGCTTCTTGAAAGCGTTCCAGCAGCTTCAGCGACCAGTTGTAATCTGCAACAGGCCGTACCTGACGGTAAATACGCGGTACGTTTTCCAGGTTATGGTTGAACACATCTGGTGGCGTTGCAGTCAGAatat                                                                                  <  2:265045/250‑1 (MQ=255)

                                                                                                                                                                    gTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCATTGGTTTCACCCAGTCCCACCATCAAACCAGACTTTGTCGGGATTTCCGGATGCGCTTCTTTAAAGCGTTCCAGCAGCTTCAGCGACCAGTTGTAATCTGCACCAGGCCGTACCTGACGGTAAATACGCGGTACGTTTTCCAGGTTATGGTTGAACACATCTGGTGGCGTTGCAGTCAGAATATCCAGAGCACG‑ATCCATACg                                                              <  1:73506/251‑1 (MQ=255)

                                                                                                                                                                               ccggcccctgtcgctcagtcccTCAATAATTTCTTCATTGGTTTCACCCAGTCCCCCCATCAAAGCAGACTGTTTCGGGATTTCCGGATGCGCTTCTTGAAAGCGTTCCAGCAGCTTCAGCGACCAGTTGTAATCTGCACCAGGCCGTACCTGACGGTAAATACGCGGTACGTTTTCCAGGTTATGGTTGAACCCATCTGGTGGCGTTGCAGTCAGAATATCCAGAGCACG‑ATCCATACGACCGCGGaaag                                                   <  2:164935/231‑2 (MQ=255)

                                                                                                                                                                                    gcAGGTCGCGCATTACCTCAATAATTTCTTCATTGGTTTCACCCAGTCCCACCATCAAACCAGACTTTGTCGGGATTTCCGGATGCGCTTCTTTAAAGCGTTCCAGCAGCTTCAGCGACCAGTTGTAATCTGCACCAGGCCGTACCTGACGGTAAATACGCGGTACGTTTTCCAGGTTATGGTTGAACACATCTGGTGGCGTTGCAGTCAGAATATCCAGAGCACG‑ATCCATACGACCGCGGAAATCCGGc                                              <  1:86823/251‑1 (MQ=255)

                                                                                                                                                                                           gcgcATTACCTCAATAATTTCTTCATTGGTTTCACCCAGTCCCACCATCAAACCAGACTTTGTCGGGATTTCCGGATGCGCTTCTTTAAAGCGTTCCAGCAGCTTCAGCGACCAGTTGTAATCTGCACCAGGCCGTACCTGACGGTAAATACGCGGTACGTTTTCCAGGTTATGGTTGAACACATCTGGTGGCGTTGCAGTCAGAATATCCAGAGCACG‑ATCCATACGACCGCGGAAATCCGGCACCagag                                       <  1:345951/251‑1 (MQ=255)

                                                                                                                                                                                                                 tcATTTGTTTCACCCAGTCCCACCATCAATCCAGACTTTGGCGGGATTTCCGGATTCCATTCTGTAAAGCGTTCAAGCAGCTGCAGCGACCAGTTGTAATCTGCACCAGGCCGTACCTGACGGTAAATACGCGGTACGTTTTCCAGGTTATGGTTGAACACATCTGGTGGCGTTGCAGTCAGAATATCCAGAGCACG‑ATCCATACGACCGCGGAAATCCGGCACCAGAGTTTCAATTTTGATGTGCggg                   <  2:234156/249‑1 (MQ=255)

                                                                                                                                                                                                                      ggTTTCACCCAGTCCCACCAGCAAACCAGTCTTTGTCGGGATTTCCGGATGCGCTTCTTTATAGCGTTCCAGCAGCTTCAGCGACCAGTTGTAATCTGCACCAGGCCGTTCCTGACGGTAAATACGCGGTACGTTTTCCAGGTTATGGTTGAACACATCTGCTGGCGTTGCATGCAGAATATCCAGAGCTCG‑ATCCATACGACCGCGGAAATCCGGCACCAGAGTTTCAATTTTGATTTGCGGTCtttttt            <  2:223632/251‑1 (MQ=255)

                                                                                                                                                                                                                       gTTTCACCCAGTCCCACCATCAAACCAGACTTTGTCGGGATTTCCGGATGCGCTTCTTTAAAGCGTTCCAGCAGCTTCAGCGACCAGTTGTAATCTGCACCAGGCCGTACCTGACGGTAAATACGCGGAACGTTTTCCAGGTTATGGTTGAACACATCTGGTGGCGTTGCAGTCCGAATATCCAGAG‑ACGGATCAATACGACCGCGGAAATCCGGCAACAGAGTTTCAATTTTACTTTGCGGGCTTTTTTc           >  2:59809/1‑251 (MQ=255)

                                                                                                                                                                                                                             cccAGTCCCACCATCAAACCAGACTTTGTCGGGATTTCCGGATGCGCTTCTTTAAAGCGTTCCAGCAGCTTCAGCGACCAGTTGTAATCTGCACCAGGCCGTACCTGACGGTAAATACGCGGTACGTTTTCCAGGTTATGGTTGAACACATCTGGTGGCGTTGCAGTCAGAATATCCAGAGCACG‑ATCCATACGACCGCGGAAATCCGGCACCAGAGTTTCAATTTTGATTTGCGGGCTTTTTTCCCGAAt     >  1:156712/1‑251 (MQ=255)

                                                                                                                                                                                                                             cccAGTCCCACCATCAAACCAGACTTTGTCGGGATTTCCGGATGCGCTTCTTTAAAGCGTTCCAGCAGCTTCAGCGACCAGTTGTAATCTGCACCAGGCCGTACCTGACGGTAAATACGCGGTACGTTTTCCAGGTTATGGTTGAAAACATCTGGTGGCGTTGCAGTCAGAATATCCAGAGCACG‑ATCCATACGACAGCGGAAATCCGGCACCAGAGTTTCAATTTTGATTTGAGGGCTTTTTTCCCGAAt     >  1:172412/1‑251 (MQ=255)

                                                                                                                                                                                                                                 gTCCCACCATCAAACCAGACTTTGTCGGGATTTCCGGATGCGCTTCTTTAAAGCGTTCCAGCAGCTTCAGCGACCAGTTGTAATCTGCACCAGGCCGTACCTGACGGTAAATACGCGGTACGTTTTCCAGGTTATGGTTGAACACATCTGGTGGCGTTGCAGTCAGAATATCCAGAGCACG‑ATCCATACGACCGCGGAAATCCGGCACCAGAGTTTCAATTTTGATTTGCGGGCTTTTTTCCCGAATGGc  >  1:10740/1‑250 (MQ=255)

AATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGCTTTCATTTCGTCGAACTCATCCGGGCTAACGTAACGTTGAACCGGCAGGTGATGGCGGCTTGGCTGCAAATATTGCCCCAGCGTTAACATCGTCACACCATGACGGCGCAGGTCGCGCATTACCTCAATAATTTCTTCATTGGTTTCACCCAGTCCCACCATCAGACCAGACTTGGTCGGGATTTCCGGATGCGCTTCTTTAAAGCGTTCCAGCAGCTTCAGCGACCAGTTGTAATCTGCACCAGGCCGTACCTGACGGTAAATACGCGGTACGTTTTCCAGGTTATGGTTGAACACATCTGGTGGCGTTGCAGTCAGAATATCCAGAGCACG‑ATCCATACGACCGCGGAAATCCGGCACCAGAGTTTCAATTTTGATTTGCGGGCTTTTTTCCCGAATGGC  >  NC_000913/659280‑659754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`