Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,674,147	T→A	Q181L (CAG→CTG)	yphB ←	putative aldose 1‑epimerase YphB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,674,147	0	T	A	95.7%	62.4 / NA	23	Q181L (CAG→CTG)	yphB	putative aldose 1‑epimerase YphB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (16/6);  minor base C (1/0);  total (17/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGAAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTG‑CGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCTGCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACT  >  NC_000913/2673924‑2674391                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     aaccGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGAAATAACTCGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTG‑CGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGTCTGGTTAAAATCCAGTTCCTGCGGTAGCAGCTCGCAAAACTCACCCgccagcca                                                                                                                                                                                                                             <  1:106380/246‑1 (MQ=255)  aTCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTAACAAACGCCGGGTCGGAAACAAAGATGAAATAACACGGTGCAGGTGGCGTCGGTTCCATGATGATGGCATAACCTTCCTG‑CGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCAGCTCGCCAAACTCACCCGCCAGCCACTg                                                                                                                                                                                                                          >  1:119182/1‑251 (MQ=255)             ccagaAGAAATCAAACGAATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGAAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTG‑CGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCAACCACTGGCGCGGCCACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGGAGCCGCTCGCAAAACTCACCCGCCAGCCACCGACCCCGCTccc                                                                                                                                                                                                               >  2:150524/2‑250 (MQ=255)                              cATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGAAATAACACGGTTCAGATGGCGTCGTTTCCATGATGATGGCATAACCTTCCTG‑CGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACAGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCAGCTCGCAACACTCACCCGCCAGACACTGATcccccccccgacactaactgcccccc                                                                                                                                                                                              >  2:244224/1‑228 (MQ=255)                                     tttATCAAACGCAGGGTCGGAAACAAAGATGAAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTG‑CGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCAGCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCt                                                                                                                                                                                       >  1:264371/1‑251 (MQ=255)                                           aaaCGCAGGGGCTGAAACAAAGATGAAATTACACGGTGCAGGTGGCGTCGTTCCCATGATGAGGGC‑TTACCCTCCTGGCGGCTGCTCTATGCGGGCCTTCCCTTTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGTGGGCTGGTTAAAATCCAGTTCCGGCGGTATCATCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATcc                                                                                                                                                                                 <  1:226813/251‑1 (MQ=255)                                                cAGGGTCGGAAACAAAGATGAAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCGG‑CGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCAGCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCAAGTAACCGCTCGCCTGTGCCTGAATCCGCGtt                                                                                                                                                                            <  1:190375/251‑1 (MQ=255)                                                   ggTCGGAAACAAAGATGAAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGCTCGCATAACCTTCCTG‑CGGCTGCTCGACGCGGGCCTGACCATTCCATCCCGCAAAACCCTTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTACAATCCCGTTCCTGCGGTACCAGCTCGCAACACTCACCCCCCACCCACTGCCCCCGCCCCAGCCAGCAACCCCTCGCCTGTCCCTGACTCCGCGtttcc                                                                                                                                                                         >  2:155269/1‑249 (MQ=255)                                                      cGGAAACAAAGATGAAATAACACGGGGAAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTG‑CGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAACCCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCAGCTCGCAAAACTCCCCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTgcgg                                                                                                                                                                       <  1:320171/250‑1 (MQ=255)                                                              aaGATGAAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTG‑CGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCAGCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACgg                                                                                                                                                              >  1:2722/1‑251 (MQ=255)                                                              aaGATGAAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTG‑CGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCAGCTCGCAAAACTCAACCGCCAGCCACTGCTCCCGCTCCAGCCAGTAAACTCCCGCCTGTGCCTGAATCCGCGGTTGCGGCGACAAc                                                                                                                                                                >  2:326611/1‑249 (MQ=255)                                                                         acacGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTG‑CGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCAGCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGAt                                                                                                                                                   <  1:155269/251‑1 (MQ=255)                                                                             ggTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTG‑CGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCAGCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGcc                                                                                                                                                >  1:246798/1‑250 (MQ=255)                                                                                       gCGTCGTTTCCATGATGATGGCATAACCTTCCTG‑CGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCAGCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTAcc                                                                                                                                      >  1:344712/1‑250 (MQ=255)                                                                                       gCGTCGTTTCCATGATGATGGCATAACCTTCCTG‑CGGCTGCTCGATGCGGGCCTGACAATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAAACTACAGTTCCTGCGGTAGAAGCTCGCAAAACTCACCCGCCAGCCCCTGCTCCCGCTCCAGCAAGTAACCGCTCGCCTGTGCCTGAATCCGAGTTTGCAGCGACAACGGCAAATAAGGATGCCAGCCGGCAcc                                                                                                                                      >  2:298033/1‑250 (MQ=255)                                                                                        cgtcgtTTCCATGATGATGGCATAACCTTCCTG‑CGGCTGCTCGATGCGGGCCTGACCATTCCATACGGCAAAACCATTGTTCACCCAATGACGCGGCAACGGCGCGGGCTGGTTAAAATCCAGTTGCTGCGGTAGCAGCTCGCAAAACTCACCCGACAGCCACTGCACCCGCTCCAGCCAGTAACCGATCGCATGTGCCTGAAACCGAGTTTGCGGAGACAAAGGCCAATAAGGAGGCCAGCCGGTACCa                                                                                                                                     >  2:26568/1‑250 (MQ=255)                                                                                            gtTTCCATGATGATGGCATAACCTTCCTG‑CGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCAGCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATgg                                                                                                                                >  1:346927/1‑251 (MQ=255)                                                                                                   tgatgatgGCATAACCTTCCTG‑CGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCAGCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGACTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGtc                                                                                                                         >  1:240850/1‑251 (MQ=255)                                                                                                            cATAACCTTCCTG‑CGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCAGCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCAGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCt                                                                                                                >  1:254907/1‑251 (MQ=255)                                                                                                                                                                                    cgcgGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCAGCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGCTGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAAAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTg                                         >  1:342329/1‑251 (MQ=255)                                                                                                                                                                                                       gttaaaacccctgtcccTGCGGAAGCACCTCACAAAACCCCCCCCCCACCCATTGCCCCCGCTCCAGCCATTACCCGCTCGCTTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGAGGCCAGCGGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATg                       <  1:267185/235‑1 (MQ=255)                                                                                                                                                                                                                 cAGTTCCTGCGGTAGCAGCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGGACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCCCCGTCAGCGTATCCGCCGTTAAATGAAACACCTTACTTACCCGATAGTGATAGACACCGCCGCGATGTTCATACAcc             >  1:324315/1‑250 (MQ=255)                                                                                                                                                                                                                           ggTAGCAGCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGAAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACt  >  1:224003/1‑251 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     CATCGGTTCCAGACAGAAGAAATCAAACGCATATCCTTTATCAAACGCAGGGTCGGAAACAAAGATGAAATAACACGGTGCAGGTGGCGTCGTTTCCATGATGATGGCATAACCTTCCTG‑CGGCTGCTCGATGCGGGCCTGACCATTCCATCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCTGCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGGATGCCAGCCGGTACCAAATGGCAGCGTCTCTGCCCCTTGATTGGTGACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACT  >  NC_000913/2673924‑2674391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑