| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,474,425 | T→G | K43T (AAA→ACA) | rpsL ← | 30S ribosomal subunit protein S12 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,474,425 | 0 | T | G | 96.4% | 77.7 / ‑3.4 | 28 | K43T (AAA→ACA) | rpsL | 30S ribosomal subunit protein S12 |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (13/14); total (14/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01 | |||||||||||
CATTAAGCCTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAA > NC_000913/3474176‑3474659 | cattaatcctttgacgtctccACGCCATACTTGGACAAAGCCTGTTTACGGTCTTTAACGCCGGAGAAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGTTCTCTAACACGACCGCCACGTATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCCCCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGtttttt < 2:34113/231‑1 (MQ=255) tAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGgt > 1:328442/1‑250 (MQ=255) gCTTCACGCCATACTTGGAACGAGCCTGCTTACGGACTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTACGGTTTTGTAGGAGTGGTAGtatat > 2:264195/1‑251 (MQ=255) tGGAACGAGCATGGTTACGGTATTTACCGCCGGAGCAGTAAAGCGCACCACGTACGGTGTGGTAACGAACCACCGGGAGGTCTTTAACACGCCCGCCACGGATCAGCATCTCGGAGAGCTCCTGCAGGTTGTGACCTTCCCCAACGATGTAGGAAATCACTTCGAACCCGTTAGTCAGACGAACCCGGCCGACTTTACGCAGCGCGGAGTTCGGTTTTGTGGGAGAGGTAG‑ATATAACACGGGTACAtccg > 2:228518/1‑248 (MQ=255) ccttcTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCTCCCTGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATTCTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATA‑CACGAGTACATACGCCACGtttt < 1:51128/247‑1 (MQ=255) ttAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTc > 1:71645/1‑251 (MQ=255) ccGGAGCAGTCAAGAGCACCACGTACGGTGTGGTCACGAACACCCGGGAGGTCTTTAACACGGCCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGCGACCTTCACCACCGATATAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGGGTTCGGTTTTGTAGGCGTGGTAGTATATA‑CCCGAGAGCATAAGCCTCGTTTTTGCGGGCATGCTTCCCgcgc > 2:95271/1‑251 (MQ=255) aaggcgcCCCACGTGGGGTGTGTAAACGAACCCCGGGGAGGTGTTAATCACGACCGCCACGTATCAGGATCACTGAGGGCTCCTGCAGGTTGTGACCTGCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTGTGTAGGAGTGGGAGTATATA‑CACGAGTACATACGCCGCGTTGTTGCGGGCATGCTTCCAGCGCAGGCACGTTg < 2:339434/248‑1 (MQ=255) cccggtgcGGTGTGGTAACGAACACCCGGGAGGTCTTTACCCCTACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGc < 2:71645/244‑1 (MQ=255) gtgtGGTAACGAACACCCGGGAGGTCTTTCACACTACAGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGCCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGAGGTCGTATATA‑CCCGAGTACATACACCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCg > 2:47017/1‑251 (MQ=255) cacCCGGGAGGTCTTAAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTg < 1:105488/251‑1 (MQ=255) ggAGGTCTTTAACACGAACGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGACACTTCGAAACCGTTCGTCAGACAAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATA‑CACGAGTCCATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGAACGTTGCTTTGCGCAACTTTGCGCGCACGGGGTTTGCGTaac > 2:310276/1‑249 (MQ=255) tgctctaccgtcactgatcgtgatcaCGGATCGCTCCTGCACTGTGTGACCATCACCACCGATGGAGGAAGTCACTTCGAAACTGTTCGTCAGACGAACACTGCATCCTTTACGCAGCGCGGAGTTCGGGTTTGTCGGAGTGGTAGTATCTA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTggcga < 2:328442/229‑4 (MQ=255) gtgACCTTCACCACCGATGTAGGAAGTAACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGTGAGTACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTac > 2:168961/1‑249 (MQ=255) ccGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAAc > 2:267970/1‑251 (MQ=255) gATGTAGGAAGTCACTTCGAAACCTGTAGTCAGACGAACCCGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATaaaa < 2:238606/248‑1 (MQ=255) gATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATaaaa > 1:238606/1‑248 (MQ=255) gTAGGAAGTCAATTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTACTGGTTTTAGCTTTTGCTTCGTAAACACGAAATAAAACGTCCt > 1:331124/1‑251 (MQ=255) aaGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTcacac > 2:165626/1‑251 (MQ=255) tCGAAGCCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGa < 1:268863/250‑1 (MQ=255) aaCCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACg < 1:20645/251‑1 (MQ=255) ccgttaggaagacgaatattgCATACTGTACGCAGCGCGGAGTTCGTGTTTGTAGGAGTGTTAGTAGATA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCAGGTTGCTTTTCGCAACTTTGCGAGCACGTGGGTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGTTCTTGGTTTTAGCTTTTGCCTCGTAAACACGTAATAATACGTCTTCACACAATAGGAGGACGcc < 2:155698/231‑1 (MQ=255) ttAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCAAACAATATGAGGACGCCGa > 1:31519/1‑250 (MQ=255) gTCAGACGAACATGGCATACTTTACGCAGCGCGTAGTTCGGTTTTGTAGGAGTGGTAGTATATA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTGTAGCTTTTGCTTCTTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAAttt < 1:224463/251‑1 (MQ=255) gAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAg < 1:153318/247‑1 (MQ=255) gAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGCAGTATATA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATCGATCATGGTTTTCGCTTTTGCTTCGTAAACACGTAATAACACGTCCTCACACACTCTGTGGACGCCGAATTTTAg > 2:153318/1‑247 (MQ=255) cGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGcc < 1:91083/251‑1 (MQ=255) aTACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGaaa < 2:31519/251‑1 (MQ=255) cgcgGAGTACGGTTTTGTAGGAGTGGTAGTATATA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGCTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGaaa < 1:264195/251‑1 (MQ=255) | CATTAAGCCTTAGGACGCTTCACGCCATACTTGGAACGAGCCTGCTTACGGTCTTTAACGCCGGAGCAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATA‑CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAA > NC_000913/3474176‑3474659 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |