Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,215,347	T→A	intergenic (+138/‑131)	metA → / → aceB	homoserine O‑succinyltransferase/malate synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,215,347	0	T	A	100.0%	45.7 / NA	16	intergenic (+138/‑131)	metA/aceB	homoserine O‑succinyltransferase/malate synthase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (5/11);  total (5/11)

GGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTT  >  NC_000913/4215109‑4215597                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                           ggCGTAGTCACGGTAATTTACTGTTTTCCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCATCGCTGGATTAATCATCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTACAATg                                                                                                                                                                                                                                                 <  1:70617/250‑1 (MQ=255)                    aCTGTTTACCAACTGGCTCAACTATTAAGTCTACAAGATCACGCCATAAGATCTACGGCCCATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGAAACTCATCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTTAAATGGAAATTGtttttgtttttg                                                                                                                                                                                                                              >  2:246547/1‑250 (MQ=255)                           aCCAACTTGCTCAACTATTACGTCCACCAGATCACGCTATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTTCTAGTCGATCGTTTAGCGATTCATCACCTTACCTCAGGCTCCTTCTGGTGCCTTTTTTATTTCCGAAACGTTCCGCATCAGGTGAATAAATTTTATTCATATTGTTTTCATCAAGTTATCAATTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTa                                                                                                                                                                                                                       <  2:53304/250‑1 (MQ=255)                                        aCTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTa                                                                                                                                                                                                         <  1:276207/251‑1 (MQ=255)                                              aCGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTtgt                                                                                                                                                                                                    <  1:15624/250‑1 (MQ=255)                                                           cACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTTCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAag                                                                                                                                                                                       <  1:138128/250‑1 (MQ=255)                                                                   aCGACCTACGGCACATGAATCCTACGCTGGATTAATCTTCTGTGATAGTCTATCGTTAAGCGATTCAGCATCTATCCTCAGGCGCCTTCGGGTTCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCATCAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAAc                                                                                                                                                                               <  2:279670/250‑1 (MQ=255)                                                                                   gAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGt                                                                                                                                                              >  1:289304/1‑251 (MQ=255)                                                                                               ggATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAACCGTACCTCAGCAGGTGCATCAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTCAAATGGAAAtt                                                                                                                                                                                                                                           >  2:5512/1‑162 (MQ=255)                                                                                               ggATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAAtt                                                                                                                                                                                                                                           <  1:5512/162‑1 (MQ=255)                                                                                                        ttctGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATg                                                                                                                                         >  1:277509/1‑251 (MQ=255)                                                                                                                                             aCCTTAGGCACCTGTGGGGGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATATTGCATTTGAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTGTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGcaacaaca                                                                                                     <  2:38008/250‑1 (MQ=255)                                                                                                                                                                             ccGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATgg                                                                     <  2:83305/250‑1 (MQ=255)                                                                                                                                                                                          cagcagGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTTCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGTAGCTGCACGATGACTGAACAGTCAACTACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGGGAAGc                                                       <  1:124511/251‑1 (MQ=255)                                                                                                                                                                                                                cATAATGTTATTATCAAGTTATCAAGTATGTATAATTAAAATGGAAATTGTTTTGGATTTTGCATTTTAAAGGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGAGCCATGATGAGGAGCTGCACGTTGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCTAGCAGGAGAAGCAAATTCTTACTGCTGAAGCgg                                  <  2:225342/250‑1 (MQ=255)                                                                                                                                                                                                                                    aTCACGTATTTATAATTCAAATGGAAATTGTTTTTGATTTTGCATTTTAACTGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTgg             >  1:101879/1‑251 (MQ=255)                                                                                                                                                                                                                                               ttaattaaAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGTACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCAtttt  >  2:115847/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                           GGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTT  >  NC_000913/4215109‑4215597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑