Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,462,459 G→A Q68* (CAG→TAG)  nrdD ← anaerobic ribonucleoside‑triphosphate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,462,4590GA96.4% 84.7 / NA 28Q68* (CAG→TAG) nrdDanaerobic ribonucleoside‑triphosphate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (13/14);  minor base T (0/1);  total (13/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTAACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACATCAGCTGATTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGTTGCGGCCCTGCATCTGCTCGCTGACAACCGCGGCAACAGTGGCGCAATAATCGGCATCATCGACTTCCGCTGCTTTAGCTGCACGCAGAATCGCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATGCGGTGTCATGATCGCTCCATATTTTTAAGAACAGGTTATCCACAGA  >  NC_000913/4462213‑4462697
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              
cGTCACGCGGCAGCTGGGTCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTGTTCGTTGAGTAACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGTCATCAGCTTAttt                                                                                                                                                                                                                                            <  1:38562/251‑1 (MQ=255)
        ggctgctggTGCTGAGGTGCATATTTTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACTTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTAACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACATCAGCTAATTGTCAACTGc                                                                                                                                                                                                                                    <  2:106023/244‑1 (MQ=255)
                 gtcggACGTGCATAGTGTTTTGCCACGATCCCGGCTAGCAGGTCGAGCTGGGTTGGAATCACCTTGCTTTCTTTGTTGGCGGTTTCTTTGAGTAACGAGGCGTTGGTCTGCTCGACCAGCCCACGGATCTCCTGGTTCAGTCGACCGCGTTTTTCCCGTTCAATGTCGCGAACGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACATCAGCTAAGTTTCAACTGTGGTCTGGAt                                                                                                                                                                                                                           <  2:147527/247‑1 (MQ=255)
                          gCATAGTGTTTAGCCACGAACCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGATGCATATGCTGGCGCTTACGTTGAGTAACGAGGCGTTGGTCTGCACGCTCAGACCACGGATCTAGTGGTTCAGGCAACCGTGCTTTTAACGTTCAATGTCGCGAACGTCATGGTAATCGATGTAAGCACGATCCAGTTCCTTGTAGGGAAATGACCCCAGCTAATTTTCAACTGCGCCCGGGATCTCAtttct                                                                                                                                                                                                                  >  2:7288/1‑248 (MQ=255)
                                      gCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTAACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACATCAGCTAATTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGtt                                                                                                                                                                                                      >  1:60974/1‑251 (MQ=255)
                                                                  gggTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTAACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACATCAGCTAATTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGTTGCGGCCCTGCATCTGCTCGCTGACAAcc                                                                                                                                                                          <  1:229658/251‑1 (MQ=255)
                                                                        gAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTAACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACATCAGCTACTTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGTTGCGGCCCTGCATCTGCTCGCTGACAACCGAGGc                                                                                                                                                                     >  2:155223/1‑250 (MQ=255)
                                                                           tCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTAACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACATCAGCTAATTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGTTGCGGCCCTGCATCTGCTCGCTGACAACCGCGGCAAc                                                                                                                                                                  >  1:3413/1‑250 (MQ=255)
                                                                                           gttgGCGTTTTCGTTGAGTAACGAGGCGTTGGTCTGCTCGACCAGGCCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACATCAGCTAATTTTCAACTGCGGTCTGGATCTGATTGATATCCACCTGGTTGCGGGCCGGCATCTGCTCGCTGACAACCGCGGCAACAGTGGCGCAATAATCgg                                                                                                                                                 <  1:16036/251‑1 (MQ=255)
                                                                                                  ttttCGTTGAGTCACGAGGCGTTGGTCTGCTCGACCAGACCTCGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACATCAGCTAATTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGTCGCGGCCCTGCATCTGCTCGCTGACAACCGCGGCAACAGTGGCGCAATAATCGGCctcatc                                                                                                                                          >  2:124467/1‑251 (MQ=255)
                                                                                                      cGTTGATTAACGAGGCTGTGGTCTGCTCTACCAGACCACTGATCTCCTGGTTCCGGCGACCGCGGTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGTACCTGACATCAGCTAATTTTCAACTGCGTTCTGGATCTCATTGATATCCACCTGGTTTCGGCCCTGCATCTGCTCGCTGACATCCGCGGCAACAGTGGCGCAATAATCGGCATCATCGAc                                                                                                                                       <  1:218562/250‑1 (MQ=255)
                                                                                                            gTAACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACATCAGCTAATTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGTTGCGGCCCTGCATCTGCTCGCTGACAACCGCGGCAACAGTGGCGCAATAATCGGCATCATCGACTTCCgct                                                                                                                                <  1:155223/251‑1 (MQ=255)
                                                                                                                                  gtccagtccaCGGAGTTCCGGGTTCAGGCGGACGCGTTTTTCACGTTAAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACATCAGCTAATTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGTTGCGGCCCTGCATCTGCTCGCTGACAACCGCGGCAACAGTGGCGCAATAATCGGCATCATCGACTTCCGCTGCTTTAGCTGCACGCAGAATCg                                                                                                          <  2:85900/249‑1 (MQ=255)
                                                                                                                                       gaccaCGGATCTCCTGGTTCAGGCGACCGCGATTTTCACGTTCAATGGCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACCTCAGCTAATTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGTTGCGGCCCTGCATCTGCTCGCTGCCAACCGCGGCAACAGTGGCGCAATAATCGGCTTCATCGAATTCCGCTGCTTTAGCTGCACGCAGAATCGcttct                                                                                                     >  2:44738/1‑251 (MQ=255)
                                                                                                                                                               gACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACATCAGCTAATTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGTTGCGGCCCTGCATCTGCTCGCTGACAACCGCGGCAACAGTGGCGCAATAATCGGCATCATCGACTTCCGCTGCTTTAGCTGCACGCAGAATCGCTTCTTTGATGCGCTCTGATTTAAACGGc                                                                             >  1:87920/1‑251 (MQ=255)
                                                                                                                                                                        tgtCACTTTCAATGTCGCTATCGTGACGGTACTCGATTTATGCACGAGCCAGTTGGTTGTATTGATCTGACATCATCGAATTTTCAACTGCGTTCTGGAGCTCATTGTTATCCACCTGGTGGCGGCCCTGCATCTGCTCGCTGACAACCGCGGCAACAGTGGCGCAATAATCGGCATCATCGACTTCCGCTGCTTTAGCTTCACGCAGAATCGCTTCTTTGATGCGCTCGTATTTAACCGTCTCTTTGCa                                                                     <  2:349389/248‑1 (MQ=255)
                                                                                                                                                                              gTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACATCAGCTAATTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGTTGCGGCCCTGCATCTGCTCGCTGACAACCGCGGCAACAGTGGCGCAATAATCGGCATCATCGACTTCCGCTGCTTTAGCTGCACGCAGAATCGCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGtct                                                              >  2:82605/1‑251 (MQ=255)
                                                                                                                                                                                 aaaTGTCGCGACCGTGTCGGGCCTCTATGTAAGCACGTGCCATTGTTTTTTATGGACCTGCAACCAGCTAATTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGTTTCGGCCCTGCATCTGCTCGCTGACAACCGCGGCAACAGTGGCGCAATAATCGGCATCATCGACTTCCGCTGCTTTAGCTGCACGCAGAATCCCTTCTTTGATGCGCTCTGATTTAAACGGCTCTGTGCAGCCGTCTCg                                                            <  2:59252/249‑1 (MQ=255)
                                                                                                                                                                                          gATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACATCAGCTAATTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGTTGCGGCCCTGCATCTGCTCGCTGACAACCGCGGCAACAGTGGCGCAATAATCGGCATCATCGACTTCCGCTGCTTTAGCTGCACGCAGAATCGCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATcac                                                   >  1:264871/1‑250 (MQ=255)
                                                                                                                                                                                              gTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACATCAGCTAATTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGTTGCGGCCCTGCATCTGCTCGCTGACAACCGCGGCAACAGTGGCGCAATAATCGGCatcatc                                                                                                                                          >  2:117765/1‑159 (MQ=255)
                                                                                                                                                                                              gTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACATCAGCTAATTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGTTGCGGCCCTGCATCTGCTCGCTGACAACCGCGGCAACAGTGGCGCAATAATCGGCatcatc                                                                                                                                          <  1:117765/159‑1 (MQ=255)
                                                                                                                                                                                                gACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACATCAGCTAATTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGTTGCGGCCCTGCATCTGCTCGCTGACAACCGCGGCAACAGTGGCGCAATAATCGGCATCATCGACTTCCGCTGCTTTAGCTGCACGCAGAATCGCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATGCGgt                                            <  1:82605/251‑1 (MQ=255)
                                                                                                                                                                                                   ggTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACATCAGCTAATTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGTTGCGGCCCTGCATCTGCTCGCTGACAACCGCGGCAACAGTGGCGCAATAATCGGcatca                                                                                                                                            <  2:144929/152‑1 (MQ=255)
                                                                                                                                                                                                   ggTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACATCAGCTAATTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGTTGCGGCCCTGCATCTGCTCGCTGACAACCGCGGCAACAGTGGCGCAATAATCGGcatca                                                                                                                                            >  1:144929/1‑152 (MQ=255)
                                                                                                                                                                                                                          aGTTGTTTGTATGGACCTGACATCAGCTAATTTTCAACTGCGGTATGGATCTCATTGATATCCACCTGGTTGCTGCCCTGCATCTGCTCGCTGACAACCGCGTCAACAGTGGCGCAATAATCGGCATCATCGACTTCCGCTGCTTTAGCTGCACGCAGAATCGCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCCGCCGTCTCTTTTCATCACATGCGGTGTCATGATCGCTCCATATTTTTaca                   >  2:256256/1‑248 (MQ=255)
                                                                                                                                                                                                                              gtttgtATGGACCTGACATCAGCTAATTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGTTGCGGCCCTGCATCTGCTCGCTGACAACCGCGGCAAAAGTGGCGCAATAATCGGCATCATCAAATTCCGCTGCTTTAGCTGCACGCAGAATCGCTTCTTTGATGCGCTATGTTTTAACCGGCACTGTGCAGCCGTCTCGTTTCATAACATGCGGTGTCATGATCGATCCATATTTTTAATAAc                >  2:174416/1‑249 (MQ=255)
                                                                                                                                                                                                                               tttgtATGGGCCGGACAACAGCTAATTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGTTGCGGCCCTGCATCTGCTCGCTGACAACCGCGGCAACAGTGGCGCAATAATCGGCATCATCGACTTCCGCTGCTTTAGCTGCACGCAGAATCGCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATGCGGTGTCATGATCGCTCCATATTTTTAAGAACAg              <  2:60974/250‑1 (MQ=255)
                                                                                                                                                                                                                                             gACATCAGCTAATTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGTTGCGGCCCTGCATCTGCTCGCTGACAACCGCGGCAACAGTGGCGCAATAATCGGCATCATCGACTTCCGCTGCTTTAGCTGCACGCAGAATCGCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATGCGGTGTCATGATCGCTCCATATTTTTAAGAACAGGTTATCCACAGa  <  1:67027/249‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              
CGTCACGCGGCAGCAGGTGCTGACGTGCATAGTGTTTAGCCACGATCCCGGCCAGCAGGTCGCGCTGGGTTGGAATCACCTTGCTGTCTTTGTTGGCGTTTTCGTTGAGTAACGAGGCGTTGGTCTGCTCGACCAGACCACGGATCTCCTGGTTCAGGCGACCGCGTTTTTCACGTTCAATGTCGCGATCGTGACGGTACTCGATGTAAGCACGAGCCAGTTGTTTGTATGGACCTGACATCAGCTGATTTTCAACTGCGGTCTGGATCTCATTGATATCCACCTGGTTGCGGCCCTGCATCTGCTCGCTGACAACCGCGGCAACAGTGGCGCAATAATCGGCATCATCGACTTCCGCTGCTTTAGCTGCACGCAGAATCGCTTCTTTGATGCGCTCTGATTTAAACGGCACTTTGCAGCCGTCTCGTTTCATCACATGCGGTGTCATGATCGCTCCATATTTTTAAGAACAGGTTATCCACAGA  >  NC_000913/4462213‑4462697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: