Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	909,094	G→A	L67L (CTC→CTT)	ltaE ←	low‑specificity L‑threonine aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	909,094	0	G	A	100.0%	54.3 / NA	18	L67L (CTC→CTT)	ltaE	low‑specificity L‑threonine aldolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/8);  total (10/8)

AGGTATTCCCGCGGCAACACTTTGCCGTTGTGGGTGTTTT‑CCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCTTCTTTACCGGAAAGCTCTGCTGCGTAGTCCTGCAGAGCATTAACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATT‑ATGACGGGAAATGCCACCCTTTTTACCT  >  NC_000913/908849‑909336                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    aGGTTTTCCCGTCGCAACACCTTTCCGTGTTGGGTTTTTT‑CCGGCCTGAGTATTTGGGGGCGGGCGAAAGTGTTATCTTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGTCTCTATGGGTTGCGGTTAAATCCTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTAAGCag                                                                                                                                                                                                                                                <  1:167992/251‑1 (MQ=255)              gCAACACTTTGCCGTTGTGGGTGTTTT‑CCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTAAGCAGAGCGACCAGGTTg                                                                                                                                                                                                                                   <  1:50055/251‑1 (MQ=255)               aaaCACTTTGCCGTTGTGG‑TGTTTTTCCAGTCTGATTAATTTGGTGCGGGGGAAATGGTGATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGTGAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTAAGCAGAGCGACCAGGTTgg                                                                                                                                                                                                                                  <  1:309419/250‑1 (MQ=255)                   acTTTGCCGTTGTGGGTGTTTT‑CCAGACTGAGTAATTTGTTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTAAGCAGAGCGACCAGGTTGGCCTg                                                                                                                                                                                                                              >  1:303486/1‑251 (MQ=255)                                                 gAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTAAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGctt                                                                                                                                                                                                 >  1:47186/1‑251 (MQ=255)                                                                                                 cGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGTCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTAAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCTTCTTTACCGGAAAGCTCTGCTGCGTAGTCCTGCAGAGCATTAACGGTAg                                                                                                                                                 <  1:91966/251‑1 (MQ=255)                                                                                                                   tAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTAAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCTTCTTTACCGGAAAGCTCTGCTGCGTAGACCTGCAGAGCATTAACGGTAGGGTCGTCACCGTAAAcgt                                                                                                                               >  1:192437/1‑251 (MQ=255)                                                                                                                      cGTGCCGTCGGCAGACGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGCCCGACAATATACTCTTCGCCGCGTTCGCAGTGACTAAGCAGAGCGACCAGGTTGGACTGAGTGCCGGTAGGCAGAAAAATGGCGGCTTCTTTACCGGAACGCTCTGCTGCGTAGTCATGCAGAGAATTAACGGTAGGGTCGTCCCCGTAAActacgt                                                                                                                            >  2:302211/1‑246 (MQ=255)                                                                                                                                        gTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTAAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCTTCTTTACCGGAAAGCTCTGCGGCGTAGTCCTGCCGAGCCTTAACGGTCGGGTCGTCTCCGTAAACGGCGTCCCCAACCCGGGCGGcc                                                                                                           >  2:93642/1‑250 (MQ=255)                                                                                                                                            aTGGGTTGCGGTGGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTAAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCTTCTTTACCGGAAAGCTCTGCTGCGTAGTCCTGCAGAGCATTAACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCcatca                                                                                                       <  1:224205/250‑1 (MQ=255)                                                                                                                                                    cGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTAAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCTTCTTTACCGGAAAGCTCTGCTGCGTAGTCCTGCAGAGCATTAACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCgg                                                                                              >  1:126462/1‑250 (MQ=255)                                                                                                                                                     ggTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCACGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTAAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCTTCTTTACCGGAAAGCTCTGCTGCGTAGTCCTGCAGAGCATTAACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCCTCATCGCTTCg                                                                                               >  1:12675/1‑249 (MQ=255)                                                                                                                                                                 ccaagtgccgccgAGCCACCGGCTTAAAACAGATAGTTAAGCGCGGACTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTAAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCTTCTTTACCGGAAAGCTCTGCTGCGTAGTTCCGCAGAGAATTAACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGTCATCATCGCTTCGAGCATGGCGCGGCt                                                                                 <  1:119454/244‑1 (MQ=255)                                                                                                                                                                     gacccgccgCGCCTCCGGCTTCAATCAGATAGTTATGCGCGGCCTGACCGACAATATACCCTTCGCCGCGTTCGCATTGACTAATCAGAGCGACTAGGTTGGCCTGATGGCCGGGAGGCAGAAAAATGTCGGCTTCTTTACCGGAAAGCTCTGCTGCGTTGTCCTGCGGAGCATTAACGGTAGGGTCGTCTCCGGAAACGTCGTGCCCAACCGGGGCGGTCATCATCGCTTCGAGAATGGCGCGGCTTGGt                                                                             <  2:47186/248‑1 (MQ=255)                                                                                                                                                                      cACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTAAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCTTCTTTACCGGAAAGCTCTGCTGCGTAGTCCTGCAGAGCATTAACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTgggc                                                                            >  2:79461/1‑249 (MQ=255)                                                                                                                                                                                                                cTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTAAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCTTCTTTACCGGAAAGCTCTGCTGCGTAGTACTGCAGAGCATTAACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGCGCATGGCGCGGCTTGGTCGGGTAACGGAACTACTGCGTAAATCCATCATGGCATGTCCtt                                  >  2:137136/1‑251 (MQ=255)                                                                                                                                                                                                                     cGACAATATACTCTTCGCCGCGTTCGCAGTGACTAAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCTTCTTTACCGGAAAGCTCTGCTGCGTAGTCCTGCAGAGCATTAACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTtatt‑at                            <  1:79461/251‑1 (MQ=255)                                                                                                                                                                                                                                               cAGTGACTAAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCTTCTTTACCGGAAAGCTCTGCTGCGTAGTCCTGCAGAGCATTAACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGTCCATAATCGCTTCGAGAATGGCTCGGCTTGGTCGGGTGACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTTTTTATG‑CAGGAAATGCCCCCCttttatcct  >  2:104222/1‑246 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    AGGTATTCCCGCGGCAACACTTTGCCGTTGTGGGTGTTTT‑CCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAGTGCCGGTAGGCAGAAAAATGGCGGCTTCTTTACCGGAAAGCTCTGCTGCGTAGTCCTGCAGAGCATTAACGGTAGGGTCGTCTCCGTAAACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATT‑ATGACGGGAAATGCCACCCTTTTTACCT  >  NC_000913/908849‑909336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑