Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	727,761	C→T	E324E (GAG→GAA)	kdpA ←	K(+) transporting P‑type ATPase subunit KdpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	727,761	0	C	T	100.0%	133.1 / NA	45	E324E (GAG→GAA)	kdpA	K(+) transporting P‑type ATPase subunit KdpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/23);  total (22/23)

ACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACC‑GCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTGGTCAGTGCGGTTGGGTTTTCA  >  NC_000913/727536‑728001                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 aatcctcatcaGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACC‑GCCGAACCCCACTTCACCAATTTGCATCAGCCACATCGGCCCCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCCCA‑GGGAGCCGCCGTCGTCACGACCGCAACCAGGCTACTGACCAGCACGCCGAAACGGATTTCATTCCCTTACATATTgtagctgc                                                                                                                                                                                                                         >  2:289459/6‑244 (MQ=255)  ccAATCATCAGCCCGGCAATAAACACCGCAAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACC‑GCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCCCCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACAAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATgctgct                                                                                                                                                                                                                        >  1:336933/1‑251 (MQ=255)  cacaccaccaGCCCGGCAAAAAACACCCCCAGCAGTAAAAACACAAACATACAAAAAAGACAAGCACCAACACC‑GCCGAACACCACTTCCCCAATTGGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATgctgct                                                                                                                                                                                                                        <  2:100796/243‑1 (MQ=255)   aaatatatatcctggaataagaccccGCCAGCAGGACAACCATCTACATGCCGTAAAGACCAGAACCGACACC‑GCCGAACGCCACTTCTCCAATTTGCATCAGCCACAGCGGCACCATGCCTCCGAGAGCGGTAAACGAATCATGCATCTCAAGCACCGCGCCACA‑GGAAGCCGCCTTCGTCACGACCGCATACAGGCTAATGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGctgctg                                                                                                                                                                                                                       <  1:46677/227‑1 (MQ=255)     atcatcAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCCGAACCGACACC‑GCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGCCCAGCACGCCGCAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTc                                                                                                                                                                                                                     >  1:219969/1‑251 (MQ=255)                       acccccGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACC‑GCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCcagcag                                                                                                                                                                                                   <  1:8788/247‑1 (MQ=255)                            cGCCAGCAGGACAAACAGCACCATGCCGTAAAGACCAGAACCGACCCC‑GCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAg                                                                                                                                                                                              <  1:216716/251‑1 (MQ=255)                                agcagGACAAACAGCACCATGCCGTAAAGACCAGAACCGACCCC‑GCCGAACACCCCTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGAtt                                                                                                                                                                                          <  1:289459/251‑1 (MQ=255)                                 ccagcaCAAAGAGCATCATGCCGTAAAGCCCAAAACCG‑CACCCGCCGAACACCACTTCACCAATTTGCAACATCCCCATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTGTACCTTCCATATTGATGCGGCTGTCCGTGCCCATTGCCAGCATATGAGGATTa                                                                                                                                                                                         <  2:219969/246‑1 (MQ=255)                                                      cGTAAAGACCAGAACCGACACC‑GCCGAACCCCACTTCACCAATTAGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAACCCTGCCTCCCTATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCCAACAGGCTGCTGACAAGCACGCCGCAACGGCTTTCTTTACCTTCCATATTGATGCTGATTTCCGTGCCCAGAGCCAGCAGATTCGGATACCCCTGAACTGCTGCCCACATc                                                                                                                                                                    >  2:163362/1‑251 (MQ=255)                                                                  aaCCGACACC‑GCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACg                                                                                                                                                        >  1:128567/1‑251 (MQ=255)                                                                  aaCCGACACC‑GCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAAAGACTCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGAAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCATCCATATTGATGCTGCTGTCCGTGACCAGTGCCAGCCGATGAGGCTTACCCTGCACTTCTGCCCACATCACAACGCCTACg                                                                                                                                                        >  1:332886/1‑251 (MQ=255)                                                                          cc‑gccgAACCCCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGAc                                                                                                                                                <  1:59458/251‑1 (MQ=255)                                                                              ccgAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGTCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTc                                                                                                                                                                                                                                       <  1:110980/161‑1 (MQ=255)                                                                                gAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTCCCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACaaaa                                                                                                                                            >  1:53083/1‑250 (MQ=255)                                                                                    accacTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAACATCAc                                                                                                                                       >  1:63413/1‑251 (MQ=255)                                                                                                aTTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCAATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGCTGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGccc                                                                                                                            >  1:86137/1‑250 (MQ=255)                                                                                                          gCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATg                                                                                                                 <  1:92473/251‑1 (MQ=255)                                                                                                              caTCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGt                                                                                                                                                                                                                  >  2:11425/1‑150 (MQ=255)                                                                                                              caTCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGt                                                                                                                                                                                                                  <  1:11425/150‑1 (MQ=255)                                                                                                                 cGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATAACTGACATCGCCCACAGCAACATGCGCCCCt                                                                                                          >  1:102727/1‑251 (MQ=255)                                                                                                                              ccaagaacGGTAAACAAATCCAGCACCGCAATCACCGCGC‑ACAAGGAGTCCGCCGTCGTCCCGACCGCGATCAGTCGACTGACCTGCGCGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACGTCTGCACACATCGCCTCGCCTACGCAGAGGACAAAAATCACTGTCAGCTTCCACAGCAGCATGCTCACCTGGCGGCGATCg                                                                                               <  2:332886/242‑1 (MQ=255)                                                                                                                                 agagCGGTAAACGAATCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCAt                                                                                          <  1:329079/251‑1 (MQ=255)                                                                                                                                   agCGGTAAACGAATCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCa                                                                                        <  1:57765/251‑1 (MQ=255)                                                                                                                                         aaaCGAAGCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCGCTTCa                                                                                   <  1:108536/250‑1 (MQ=255)                                                                                                                                              aaTCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAg                                                                                                                                                     <  1:65414/179‑1 (MQ=255)                                                                                                                                              aaTCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCAAGCCTACGCAg                                                                                                                                                     >  2:65414/1‑179 (MQ=255)                                                                                                                                                 cattcATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCCCGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGc                                                                           <  2:106409/246‑1 (MQ=255)                                                                                                                                                      aTCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGca                                                                     <  1:111726/251‑1 (MQ=255)                                                                                                                                                             cccccGCGCCCCC‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTGCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGcc                                                              <  2:53083/248‑1 (MQ=255)                                                                                                                                                                cctgcccACA‑GGAAGCCGCCTTCGCCACGACCGCCATCAGGCTACTTACCAGACCGCCGAAACCGCTTTCTTTTCCTTCCATTTTGATGCTGCTCTCCGTGCCCAGTGCCAGTAGATGAGGATTACCCTTAACTCCTCCCCACATCACCTCGCCTACGCAGATGAAAAAAATCGCTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCTATCGCCCATCACTGCACCAAAGGCAAAGCACAGCGCCGtt                                                           <  2:183244/246‑1 (MQ=255)                                                                                                                                                                        ca‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCa                                                    >  1:201647/1‑250 (MQ=255)                                                                                                                                                                               gccgccGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCCCGCCTACGCAGATGACAAAAATCCCTGACATCGCCCAACGCAACATGCGCCCCTGGCGGCGATCGCCCCACACTTCACCAAAGGCAAAGCCCAACGCCGTTGGGATCaaga                                                 >  2:176455/1‑247 (MQ=255)                                                                                                                                                                                    acccgtcacacccGCAAACAGGCTACTGACAAGCACTCCGAAACGGCTTTCTTTACGTTTCATATTGAGGCTGCTGTTCGTGCCCAGTGCCAGCAGATGAGCATTTCCCTGAATTTCCTCCCCCATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACACGCGCCCCGGGCGGCTATCGCCCATCTCTTCACCAAAGGCAAAGCACATCGCCGTTGGGATCaagttcatcgccg                                        <  2:325859/240‑11 (MQ=255)                                                                                                                                                                                     gacgtcCCGAGCGCAAACAGGCTACTGCCCCGCACGCCGAAACCGCTTTCCTTCCCCTCAATATTGATGCTGCTGTCCGGGCCCAGTTCCAGCAGATGAGGAGTACCCTTAACTTCTGCCCACATCACCACGACTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGAGGCGATCGACCAGCACTCCACCAAAGGCAAAGCACAGCGCAGTTGGGAGCAAGAATATCGCCAg                                       <  2:281206/249‑1 (MQ=255)                                                                                                                                                                                        gtcACGACCGCAAACAGGCTACTGACCAGCACGCCGAATCGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGCTCAAGAAGATCGCCa                                        >  1:325859/1‑247 (MQ=255)                                                                                                                                                                                        gtcACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACCGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCAt                                    >  1:300283/1‑251 (MQ=255)                                                                                                                                                                                             gACCGAAAACAGGCTACTGAACAGCACGCCGAAACGGCTTTATTTACCTTCCATATTGATGCTGCTGTCCGTACCCAGTGCCCGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACCTCGCACACAGCAACATGCGCCCCTGGCGACAATCGCCCATCACTTCACCAACGACAAAGCCCGGCGCCGATGGGATCAAGAAGATCGCCAgaacctgc                                >  2:247342/1‑243 (MQ=255)                                                                                                                                                                                                cGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTTTATTTACCTTCCATATTGATGCTGCTGTCCGAGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCGAAGGCAAAGAACAGCGCCGTTGGAATCAAGAAGGTAGCCAGCATCTGCACg                             >  2:335297/1‑250 (MQ=255)                                                                                                                                                                                                   aaaCAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTTCCTTCCATAGTGATGCTTCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGTAACATGCGCCCCTGGCGGCGATCTCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGt                         <  1:163362/251‑1 (MQ=255)                                                                                                                                                                                                      cAGGCTACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTgg                      >  1:247890/1‑251 (MQ=255)                                                                                                                                                                                                           tACTGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCAAGCATCTGCACGAAGTTGGTCAGt                 >  1:181659/1‑251 (MQ=255)                                                                                                                                                                                                           caCTGAACAGCACGCAGAAACGGCTTTCGTTACCATCCATCTTGATGTAGCTGTCCCTGCCCCGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACTCAGATGACAAAATTCCCTGACATCGCCCACAGCAACATGCGCCTCTGGCGGCGATCGTCCATCACTTCACCAAAGGCAAAGCACAGCTCCTTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTGGTCAGt                 <  2:88941/250‑1 (MQ=255)                                                                                                                                                                                                              tGACCAGCACGCCGAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACACAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTGGTCAGTGCg              >  1:305760/1‑251 (MQ=255)                                                                                                                                                                                                                           gAAACGGCTTTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATAACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCACCATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCACGAAGATCGCCAGCATCTGCACGACGTTGGTCAGCGCGGTTGGGTTTTCa  >  2:278627/1‑250 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 ACCAATCATCAGCCCGGCAATAAACACCGCCAGCAGGACAAACAGCATCATGCCGTAAAGACCAGAACCGACACC‑GCCGAACACCACTTCACCAATTTGCATCAGCCACATCGGCACCATGCCACCGAGAGCGGTAAACGAATCATGCATCGCAATCACCGCGCCACA‑GGAAGCCGCCGTCGTCACGACCGCAAACAGGCTACTGACCAGCACGCCGAAACGGCTCTCTTTACCTTCCATATTGATGCTGCTGTCCGTGCCCAGTGCCAGCAGATGAGGATTACCCTGAACTTCTGCCCACATCACCACGCCTACGCAGATGACAAAAATCACTGACATCGCCCACAGCAACATGCGCCCCTGGCGGCGATCGCCCATCACTTCACCAAAGGCAAAGCACAGCGCCGTTGGGATCAAGAAGATCGCCAGCATCTGCACGAAGTTGGTCAGTGCGGTTGGGTTTTCA  >  NC_000913/727536‑728001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑