| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,601,098 | C→A | S48S (TCG→TCT) | lsrR ← | DNA‑binding transcriptional repressor LsrR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,601,098 | 0 | C | A | 90.5% | 42.5 / ‑1.8 | 21 | S48S (TCG→TCT) | lsrR | DNA‑binding transcriptional repressor LsrR |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (8/11); total (9/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01 | |||||||||||
AAAACCAATCGCCAGCATCTGTTGTGGTTGAAGTAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACATGTTGCAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTAAAACCACGCGATCCGCGCGACCTGTTCTTCTTCACACATTCCCTGTTCTGAAATTGCCGAATCGTTGATTGTCATAATTCATTCTTCACTTTGAACATATTTAAATCTTTAATGCAATTGTTCAGTTCTTGCTCATTTATATCTGTGATGGCA > NC_000913/1600870‑1601319 | aaaaCCAATCGCCAGCATCTGTTGTGGTTGAAGTAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACATGTTGCAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGATGCCATTTCTCCAGCAATCGAGACATTTTCAAACGTGTCAGGc > 1:312428/1‑251 (MQ=255) cAATCGCCAGCATCTGGTGTGGTTGAAGTAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACATGTTGCAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCCGTACGCGAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGAGACATTTTCAAACGTGTCAGGCCgaga < 1:231675/251‑1 (MQ=255) tagTTGAAGAAAACGCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCAGCAAGCCCAGGGATCACACGGACATGTTGCAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAACGAGAGAATTAATCTGTACGCGAATAATGCCGGACTGATGCCCTTTCTCCAGAAATCGAGACATTTTCAAACTTGTCAGGCCGCGACGAtactggatatcgctct > 2:161339/3‑236 (MQ=255) ttGAAGTAAACTCATCAACATATGCGCCGCGCCTATCCACAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGCTCACCCGGACATGTTGCAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAACGAGAATTAATCTGTAAGCGAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGAGACATTTTCAAACGTGTCAGGCCGAGCCGACCGCTGATCTCGcttaggg > 2:254533/1‑246 (MQ=255) aaaGTAAACTAATCAACAGATGCGCCGCGCCGATCCCCATTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACAGGTTGCAGAGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGAGACATTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTc < 2:255743/250‑1 (MQ=255) aacgcatcaACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACATGTTGCAGCGAAAACTGACGACGTAATTGAGTGTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGAGACATTTTCAAACGTGTCAGGCCGAGAc < 2:156485/218‑1 (MQ=255) aaCTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGCTCACCCGGACATGTTGCAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGAGACATTTTCAAACGTGTCAGGCCGAGAc > 1:156485/1‑222 (MQ=255) gcgccgcgccTATCCCCAGTCGCCCACCTACATCAGCATCCGCAAGCCCAGGGATCACCCGGACATGTTGCAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGAGACATTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTaaaa < 1:65266/250‑1 (MQ=255) cccAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACATGTTGCAGCGAAAACTGACGACGCAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGAGACATTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTgg > 1:275293/1‑213 (MQ=255) ggACATGTTGCAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGAGACATTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTAAAACCACGCGATCCGCGCGACCTGTTCTTCTTCACACATTCCCTGTTCTGAAATTGCCGAATCg > 1:319332/1‑251 (MQ=255) tttGCATCGAAAACCGCCGACTTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGAGACATTTTCAAACTTGGCAGGCCGAGACGATCGCTGATCTCGCTCTTTGTCAGCCCGTCGTGATAGTAAAACCACGCGATCCTCGCGACCTGTTCTTCTTCACACATTCCCTGTTCTGAAATTGCCGAATCGTTGAt < 2:312428/249‑1 (MQ=255) cAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATGAATCTGTACGCGAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGAGACATTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTAAAACCACGCGATCCGCGCGACCTGTTCTTCTTCACACATTCCCTGTTCTGAAATTGCCGAATCGTTGATTGTCa < 1:139261/251‑1 (MQ=255) cgacgTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGAGACATTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTAAAACCACGCGATCCGCGCGACCTGTTCTTCTTCACACATTCCCTGTTCTGAAATTGCCGAATCGTTGATTGTCATAATTCAttcttc > 1:105211/1‑251 (MQ=255) aaTTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGAGACATTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTAAAACCACGCGATCCGCGCGACCTGTTCTTCTTCACACATTCCCTGTTCTGAAATTGCCGAATCGTTGATTGTCATAATTCATTCTTCACttt < 1:22168/250‑1 (MQ=255) aGTTTCATATTCCAGTCAGCCTTCAAAGCGAGAATTGATCTGTACGCGAATAATGCCGGACTGATTCCCTTTCTCCAGCAATCGAGACATTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGGCCGTCGTGATAGTAAAACCACGCGATCCGCGCTACCTGTTCTTCTTCACACATTCCCTGTTCTGAAATTGCCGAATCGTTGATTTTCATAATTCATTCTTCACTTTGAACa < 1:143989/250‑1 (MQ=255) cATATTCCAGACAGCCTTCAAAGCGAGAATGTATTGGTACGCTAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGAGACATTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTAAAACCACGCGATCCGCGCGACCTGTTCTTCTTCACACAGTCCCTGTTCTGAAATTGCCGAATCGTTGATTGTCATAATTCATTCTACACTTTGAACatagtt < 2:72432/250‑4 (MQ=255) ttCCAGACAGCCTTCCAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGAGACATTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTAAAACCACGCGATCCGCGCGACCTGTTCTTCTTCACACATTCCCTGTTCTGAAATTGCCGAATCGTTGATTGTCATAATTCATTCTTCACTTTGAACATATTTAAATc > 1:257622/1‑251 (MQ=255) cAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTAAAACCACGCGATCCGCGCGACCTGTTCTTCTTCACACATTCCCTGTTCTGAAATTGCCGAATCGTTGATTGTCATAATTCATTCTTCACTTTGAACATATTTAAATCTTTAATGCAATTGt > 1:121240/1‑251 (MQ=255) gCGAGAATTAATCTGTACGCGAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGAGACATTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTAAAACCACGCGATCCGCGCGACCTGTTCTTCTTCACACATTCCCTGTTCTGAAATTGCCGAATCGTTGATTGTCATAATTCATTCTTCACTTTGAACATATTTAAATCTTTAATGCAATTGTTCAg < 2:319332/251‑1 (MQ=255) ggaCGCGAATATTGCCGGACGGATGCCCTTCCTCCAACAATCGCGACGCTTTCAACCGTGTCAGGCCGAGACGATCGCTGATCGCGCTCTGGGTCAGCCCGTCGTGATAGTAAAACCACGCGATCCGCGCGACCTGTTCTTCTTCACACATTCCCTGTTCTGAAATTGCCTAATCGTTGATTGTCATAATTCATTCTTCACTTTGAACATATTTAAATCTTTAATGCAATTGTTCAGCTCTTGCTCATTta < 2:143110/249‑1 (MQ=255) ccGGACTGATGCCCTTTCTCCAGCAATCGAGACATTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTAAAACCACGCGATCCGCGCGACCTGTTCTTCTTCACACATTCCCTGTTCTGAAATTGCCGAATCGTTGATTGTCATAATTCATTCTTCACTTTGAACATATTTAAATCTGTAATGCAATTGTTCAGTTCTTGCTCATTTATATCTGTGATGGCa < 1:22303/251‑1 (MQ=255) | AAAACCAATCGCCAGCATCTGTTGTGGTTGAAGTAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACATGTTGCAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTAAAACCACGCGATCCGCGCGACCTGTTCTTCTTCACACATTCCCTGTTCTGAAATTGCCGAATCGTTGATTGTCATAATTCATTCTTCACTTTGAACATATTTAAATCTTTAATGCAATTGTTCAGTTCTTGCTCATTTATATCTGTGATGGCA > NC_000913/1600870‑1601319 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |