| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,113,851 | G→A | H208Y (CAT→TAT) | fpr ← | flavodoxin/ferredoxin‑NADP(+) reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,113,851 | 0 | G | A | 92.6% | 74.5 / ‑2.7 | 28 | H208Y (CAT→TAT) | fpr | flavodoxin/ferredoxin‑NADP(+) reductase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (14/11); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01 | |||||||||||
TCATCACTCATTTATCAACCGTTTGATGCCGGCATATCGCGGGATGATCAATAAGATGAGTGCGGATTTGCTCATCGTGCCGTTTATCGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATGGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTT‑CCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGTGGCAAATAGCTTAAGTCGGCGGCATAACGTGCGGCGTGCACCAGGACCAGATTTTTGAAGCGATCTAAATCTTTACC > NC_000913/4113625‑4114091 | tcatcaCTCATTTATCAACCGTTTGATGCCGGCATATCGCGGGATGATCAATAAGATGAGTGCGGATTTGCTCATCGTGCCGTTTATCGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGc > 2:52605/1‑249 (MQ=255) aTCAACCGTTTGATGCCGGCATATCGCGGGATGATCAATAAGATGAGTGCGGCTTTGCTCATCGTGCCGTTTATCGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACAATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGGAGGCCCATCGTGCtt > 1:333974/1‑251 (MQ=255) tGATGCCGGCATATCGCGGGATGATCAATAAGATGAGTGCGGATTTGCTCATCGTGCCGTTTATCGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTT‑cc < 1:52605/250‑1 (MQ=255) gggATTATCAATAAGATGAGGGCGGATTTGCTCATCGTGCCGTTTATCGATAAGTAACCGCTTACAAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGAAAATGTTTCGTCCTCTGCCGGGCCTCTTTCAGCAACTGTTGTGTAACGCGAACCATCTGTGGATTGCCGAACAGCATCCCATAGCTGGTTTATTTATTCATCAGACGGCCAATCTTGCTTTCCAGTT‑CCCCCCTTTCATTTAATGcc > 2:281969/1‑251 (MQ=255) gtaagatCAATAAGATAAGTGCGGATTTGCTAATCGAGCCGTTTATCGATAAGTAAACGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCAGCGACGTAACTGTTTCGTCATCTGCCGGGTCTCTTTAAGCAACTGTTGTGTATCACGCACCATCTGTGGTTTGCCGCACAGCATCCCATAGCTGGTTTCTTTATTAATCGGCAGGCCCATCGTGATTTACCGTT‑CCCCACTTTCAAttcaagccg > 2:204944/5‑244 (MQ=255) tAAGATGAGTGCGGATTTGCTCATCGTGCCGTTTATCGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCTGTCGGCGACGTAAATGCTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGGGCTTTCCAGTT‑CCCCACTTTCAATTAATGCCGGTAtccgtcc > 1:319221/1‑251 (MQ=255) gATGAGTGCGGATTTGCTCATCGTGCCGTTTATCGATAAGTAACCGCTTACAAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGAAAATGTTTCGACATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTCCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTT‑CCACACTTTCAATTAATGCCGGTATCCGTCCGGt > 1:345724/1‑251 (MQ=255) aTCGTGCCGTTTATCGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTT‑CCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGtt > 1:108707/1‑251 (MQ=255) aTCGTGCCGTTTATCGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTT‑CCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGtt > 1:163258/1‑251 (MQ=255) tGCCGTTTATCGATAAGTAACCGCTTACCAGTAATCCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTT‑CCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGGTTccc > 1:87745/1‑251 (MQ=255) ttATCGATACGTAACCGCTTACCAGTAATTCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCACCTGTTGTGTATCGCGCACCGTCTGTGGATTGCCGCACAACATCACATAGCTGGCTTCTTTATTCAACGGCAGGCCAATCGTGCTTTTCAGTTTCCCCA‑TTTCAATTACTTCCGTTATCAGTCCGGTGATTGGCCCCGCTGCCGTTTCCCGACTg > 2:118499/1‑250 (MQ=255) cTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTT‑CCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGt > 1:75803/1‑251 (MQ=255) gTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTT‑CCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGt < 1:251172/249‑1 (MQ=255) gTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTT‑CCCCACTTTCAATTAATGCCGGTAGCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGTCCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGt < 1:218645/249‑1 (MQ=255) atatGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATGGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTT‑CCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACCGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTc > 1:237809/1‑251 (MQ=255) atGGCCCGTTCGTCGTCGTAAATGTTTCGTCACCTGCCTGGTCTCTTTCCGCAACTTGTGTTTTTCGCGCACCATCTGTGGATTGCCACTAAGCAACACATTGCTGGTTTCTTTATTCATCTGCAGGCCCATCGTGCTTCCCAGTT‑CCCCTCTTTCAATTAATGCCGGTATCCGTCCGGTGATCGACCCCGCTGCCGTTGCCTGACTGACCACCGGCTGAATGCGCAGTTTTCCTTCGTATCGCTTTTcc < 2:108707/250‑1 (MQ=255) ggCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTT‑CCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATTCGCAGTTTTCCTTCGTAGCGTTTTTCCAGt < 1:314650/251‑1 (MQ=255) aCGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTT‑CCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTg < 1:108582/204‑1 (MQ=255) aCGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCAAGTT‑CCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGTg < 1:43210/251‑1 (MQ=255) aaaTGTTTCGTCACCTGCCGGGTCTCTCTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATGGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTT‑CCCCACTGTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACCGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGTGGCaa < 2:237809/251‑1 (MQ=255) ttCGTCATCTGCCGGGTCTCTTTCAGCATCTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTT‑CCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGttttt > 1:109246/1‑223 (MQ=255) gggTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTT‑CCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGTGGCAAATAGCTTAAGTCggcggc > 1:260305/1‑250 (MQ=255) gttgtGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTT‑CCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGTGGCAAATAGCTTAAGTCGGCGGCATAACGTGCGGCGTGCAc < 1:281969/250‑1 (MQ=255) cACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTT‑CCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGTGGCAAATAGCTTAAGTCGGCGGCATAACGTGCGGCGTGCAc > 1:316005/1‑237 (MQ=255) cACCATCTGTGGATGGCCGCACAGCATCACATAGCTGGGTTCGTTATTCATCGGCAGGCCAAACGTGCTTTCCAGTT‑CCCCACTTTCAATGAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCTACTTACCACCGTCTGAATGCGCATTTTTCCTGCGTAGCGTTTTTCCAGTTCCTGCATCAGTGCCAAATAGCTTAAGTCGGCGGCATAACGTGCGGCGTGCAc < 2:316005/237‑1 (MQ=255) acTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTT‑CCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGTGGCAAATAGCTTAAGTCGGCGGCATAACGTGCGGCGTGCACCAGGACCAGATTTTTGAAg < 1:118499/250‑1 (MQ=255) gtgGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTT‑CCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGTGGCAAATAGCTTAAGTCGGCGGCATAACGTGCGGCGTGCACCAGGACCAGATTTTTGaa < 1:259672/247‑1 (MQ=255) aGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTT‑CCCCACTTTCAATTATTGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGTGGCAAATAGCTTAAGTCGGCGGCATAACGTGCGGCGTGCCCCAGGACCAGATTTTTGAAGCGATCTAAATCTTTAcc < 1:219658/251‑1 (MQ=255) | TCATCACTCATTTATCAACCGTTTGATGCCGGCATATCGCGGGATGATCAATAAGATGAGTGCGGATTTGCTCATCGTGCCGTTTATCGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATGGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTT‑CCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGTGGCAAATAGCTTAAGTCGGCGGCATAACGTGCGGCGTGCACCAGGACCAGATTTTTGAAGCGATCTAAATCTTTACC > NC_000913/4113625‑4114091 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |