Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,337,394	A→G	S522G (AGC→GGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,337,394	0	A	G	100.0%	62.4 / NA	22	S522G (AGC→GGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/6);  total (16/6)

CGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGG  >  NC_000913/1337168‑1337622                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                     cGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCggtgcggtgc                                                                                                                                                                                                              >  1:280179/1‑251 (MQ=255)  gCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGAGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTATGGGATACGGTTGAAACACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAACCGTAAATCCAAAAAGGCGATTTAACCGTCGGTGCGGGGCt                                                                                                                                                                                                             >  2:263140/1‑251 (MQ=255)                gAAAAAAGCCCTAACTCTGGGCCTAAAGCGGAAACCATGAGACAAAGCGACGCAGGCCCCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACAACGTATCTAGACGAACCAGGGTTTAACCTTGTGGGATACGATTGTCCCACCTGTATTGATAACTCTGGGCAGCTGACAGATACTATCGAAACGGCAATCAAAAAAGGCAGTTTAACCGTcggtgcagatatggttgtcaacagt                                                                                                                                                                                                >  2:51221/1‑231 (MQ=255)                   aaaaGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTTGGCCGGTGCCCGTTCCTATCGCAACGGCAATCACAAAAGGCGCTTTAACCGTCGGTGCGGTGCTGCCCGGCAACCGTAACt                                                                                                                                                                                            >  2:120226/1‑251 (MQ=255)                            aaCTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGcc                                                                                                                                                                                   >  1:330653/1‑251 (MQ=255)                                                               cGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGcc                                                                                                                                                <  1:69150/251‑1 (MQ=255)                                                                 gcGCTGGAACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGTGAAACTGATACCGTATCTCGACGAACTGGTGGTTAGCCTTGTGGGATACTGTTGTACCACCTGTATTGGTAACGCGTGGCCTCGGCCCGGTCCTAGCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTGAAACTAACTGGCTGGCCTc                                                                                                                                              <  2:7513/250‑1 (MQ=255)                                                                          ccGGGTTCGAAAGGAGTTTCTGATTATCTGGCAAAGGCGAAACTGACACCGTATCTCGACGAACTGGGGATTAACCTTGTGGGATACGGTTGAACCACATGTATTGGTAACTCTGGGCCGCTGCCCGATACTATCGAAACGGCAATCAAAAAAGGCGATTTAACCTTCGGTGCGTTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTACCTGGCTAGCCGcgccgacgcc                                                                                                                                     >  2:87675/1‑250 (MQ=255)                                                                              gTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGCCGACCTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGAGATTTAACCGTCGGTGCGGCGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCtggtg                                                                                                                                 >  1:35802/1‑251 (MQ=255)                                                                                   aaaGTCGTTTCTGATTGTCTGTCAAAAGCGAAACTGTCTCCGTATCGCGACGAACTGGGGTTTAACCTGGTGGGATACGGTTATACCACCTTTATTGGTAACTCTGGGCCGCGGCCCGATCCTATCGAACCGGCAATCAAAAAAGTCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCAGCCGCTGGTTAAAACTAACTGGCTGGCCTCTCCGCCGCTGGTGGTTGc                                                                                                                            <  2:123083/251‑1 (MQ=255)                                                                                         gTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATgc                                                                                                                      <  1:240042/251‑1 (MQ=255)                                                                                            tCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGCGGTTGCCTATGCGCt                                                                                                                   >  1:221529/1‑251 (MQ=255)                                                                                              tGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTgg                                                                                                                 >  1:76663/1‑251 (MQ=255)                                                                                                                                   gacgaACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGAGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAACAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGACTATCAACCTGGCTTCTGAGCCTAt                                                                            >  1:142306/1‑251 (MQ=255)                                                                                                                                       aaCTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGc                                                                        <  1:298611/251‑1 (MQ=255)                                                                                                                                          tGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGCAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCAt                                                                     >  1:278830/1‑251 (MQ=255)                                                                                                                                          tGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTAACGGCCAt                                                                     >  1:321261/1‑251 (MQ=255)                                                                                                                                            gggTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGCAATATGCATATCAACCTGGCTTCTGAGCCTATCGGCCATg                                                                    >  1:82443/1‑250 (MQ=255)                                                                                                                                                  aaCCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAACAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGACGGCCGTATCCATCCGCTGGTTACAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTAGAGGGAAATATGAATATCAATCCGGCTTCTGGGCCTATCGGCCAAGATAGCa                                                              >  2:55217/1‑250 (MQ=255)                                                                                                                                                                                     tAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCAt                          >  1:301637/1‑251 (MQ=255)                                                                                                                                                                                                 gCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATCTCTGGCCATCGGCACAAGaa               >  1:202406/1‑250 (MQ=255)                                                                                                                                                                                                             aaTCGAAACGGCAATAAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGCCAACCGTATCGTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAGCTGGCTGGCCTCGCCGCCGCTGGTGGTTGCGTATGCGCTGGCGGGAAATATGAATATCAACCTGTCTTCTGAGCCTAGCGGCCAGGATCTCAAAGGCGATCCGGTTTATCTGAGAGATATCTGGCCATCGGCACAAGAAATGGCCCGTGCgg  <  2:142306/250‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                     CGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAACCGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGG  >  NC_000913/1337168‑1337622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑