breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 4,430 C→T L233L (CTG→TTG)  thrC → threonine synthase
RA 9,398 G→A W31* (TGG→TGA mog → molybdopterin adenylyltransferase
RA 15,213 G→A G349D (GGT→GAT)  dnaJ → chaperone protein DnaJ
RA 34,178 G→A intergenic (+140/‑122) carB → / → caiF carbamoyl‑phosphate synthetase large subunit/DNA‑binding transcriptional activator CaiF
JC JC 57,276 IS2 (+) +5 bp intergenic (‑167/‑84) lptD ← / → djlA lipopolysaccharide assembly protein LptD/co‑chaperone protein DjlA
RA 64,583 G→A P400S (CCT→TCT)  polB ← DNA polymerase II
RA 71,134 G→A G250S (GGT→AGT)  araC → DNA‑binding transcriptional dual regulator AraC
RA 218,926 G→A T223T (ACC→ACT trmO ← tRNA m(6)t(6)A37 methyltransferase
RA 221,705 G→A T314I (ACT→ATT)  metN ← L‑methionine/D‑methionine ABC transporter ATP binding subunit
RA 236,865 G→A intergenic (+67/‑66) dnaQ → / → aspV DNA polymerase III subunit epsilon/tRNA‑Asp
RA 251,994 G→A intergenic (+41/‑11) dinB → / → yafN DNA polymerase IV/antitoxin YafN
RA 253,653 G→A intergenic (+492/+606) yafP → / ← pepD putative N‑acetyltransferase YafP/peptidase D
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 260,826 G→A G147S (GGC→AGC)  proB → glutamate 5‑kinase
RA 275,244 G→A intergenic (‑163/‑81) insH1 ← / → mmuP IS5 family transposase and trans‑activator/S‑methyl‑L‑methionine transporter
RA 275,932 G→A G203D (GGC→GAC)  mmuP → S‑methyl‑L‑methionine transporter
RA 276,792 G→A E26E (GAG→GAA mmuM → homocysteine S‑methyltransferase
RA 280,533 G→A S68F (TCC→TTC)  yagB ← orphan antitoxin YagB
RA 313,437 G→A intergenic (‑195/‑1854) ykgR ← / → insE1 putative membrane protein YkgR/IS3 element protein InsE
RA 323,418 G→A A361T (GCC→ACC)  ykgF → putative amino acid dehydrogenase with NAD(P)‑binding domain and ferridoxin‑like domain
RA 329,921 G→A M153I (ATG→ATA betT → choline:H(+) symporter
RA 344,072 G→A intergenic (+139/‑104) yahK → / → yahL aldehyde reductase, NADPH‑dependent/uncharacterized protein YahL
RA 372,780 G→A L222L (CTG→CTA mhpD → 2‑hydroxypentadienoate hydratase
RA 421,341 C→T P119L (CCG→CTG)  proY → putative transporter ProY
RA 426,395 C→T L87F (CTC→TTC)  tgt → tRNA‑guanine transglycosylase
RA 426,421 C→T V95V (GTC→GTT tgt → tRNA‑guanine transglycosylase
RA 434,976 T→C I110I (ATT→ATC ribE → 6,7‑dimethyl‑8‑ribityllumazine synthase
RA 475,034 G→A A43V (GCC→GTC)  tesB ← acyl‑CoA thioesterase II
RA 500,959 G→A D279N (GAT→AAT)  gsk → inosine/guanosine kinase
RA 531,873 C→T E117K (GAG→AAG)  allS ← DNA‑binding transcriptional activator AllS
RA 546,050 C→T G105D (GGC→GAC)  allD ← ureidoglycolate dehydrogenase
RA 551,779 C→T P272P (CCG→CCA purK ← 5‑(carboxyamino)imidazole ribonucleotide synthase
RA 555,049 C→T P147S (CCG→TCG)  cysS → cysteine‑‑tRNA ligase
RA 556,072 C→T G161E (GGG→GAG)  ybcI ← PF04307 family inner membrane protein YbcI
RA 556,203 C→T W117* (TGG→TGA ybcI ← PF04307 family inner membrane protein YbcI
RA 557,635 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
JC JC 568,449 IS2 (–) +5 bp coding (135‑139/333 nt) emrE → multidrug/betaine/choline efflux transporter EmrE
RA 631,633 G→A W580* (TGG→TGA cstA → pyruvate transporter CstA
RA 658,165 C→T intergenic (‑227/‑782) crcB ← / → tatE F(‑) channel protein/twin arginine protein translocation system ‑ TatE protein
RA 661,919 C→T A120A (GCG→GCA lipB ← lipoyl(octanoyl) transferase
RA 987,152 G→T intergenic (‑170/+433) ompF ← / ← asnS outer membrane porin F/asparagine‑‑tRNA ligase
RA 1,069,278 C→T V188M (GTG→ATG)  rutG ← pyrimidine:H(+) symporter
JC JC 1,105,376 IS2 (–) +5 bp intergenic (+83/‑34) csgC → / → ymdA curlin chaperone/uncharacterized protein YmdA
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,290,881 C→T L214F (CTT→TTT)  rssB → regulator of RpoS
MC JC 1,294,035 Δ141 bp [tdk] [tdk]
MC JC 1,299,499 Δ1,199 bp insH21 insH21
MC JC 1,303,073 Δ12 bp coding (175‑186/921 nt) oppB → murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,353,758 T→A D254V (GAT→GTT)  sapC ← putrescine ABC exporter membrane protein SapC
RA 1,494,388 Δ1 bp coding (684/924 nt) ydcI ← DNA‑binding transcriptional dual regulator YdcI
RA 1,517,508 G→T V40V (GTG→GTT ortT → orphan toxin OrtT
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,667,887 Δ1 bp coding (678/1221 nt) mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,744,751 Δ1 bp coding (1295/1374 nt) mdtK → multidrug efflux pump MdtK
JC JC 1,792,772 IS2 (–) +5 bp intergenic (‑41/+33) nlpC ← / ← btuD NlpC/P60 family lipoprotein NlpC/vitamin B12 ABC transporter ATP binding subunit
RA 1,978,243 G→T intergenic (‑46/+275) flhD ← / ← insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
RA 2,003,264 C→T E115K (GAA→AAA)  fliC ← flagellar filament structural protein
RA 2,032,423 G→A V219V (GTC→GTT yedJ ← putative HD superfamily phosphohydrolase YedJ
RA 2,104,837 G→A A83V (GCC→GTC)  wbbJ ← putative lipopolysaccharide biosynthesis O‑acetyl transferase WbbJ
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,229,894 C→T E164E (GAG→GAA dusC ← tRNA‑dihydrouridine(16) synthase
RA 2,242,121 A→G S283P (TCG→CCG)  yeiB ← DUF418 domain‑containing protein YeiB
RA 2,267,824 C→T intergenic (+113/‑5) yeiQ → / → yeiR putative oxidoreductase YeiQ/zinc‑binding GTPase YeiR
JC JC 2,269,876 IS5 (+) +4 bp intergenic (+309/‑100) lpxT → / → mepS lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase
RA 2,272,083 C→T S453F (TCC→TTC)  pdeN → putative c‑di‑GMP phosphodiesterase PdeN
RA 2,274,557 A→G T127A (ACG→GCG)  yejB → putative oligopeptide ABC transporter membrane subunit YejB
RA 2,286,238 C→T noncoding (28/77 nt) proL → tRNA‑Pro
RA 2,286,793 C→T pseudogene (2122/2525 nt) yejO ← adhesin‑like autotransporter YejO
JC JC 2,319,850 IS2 (+) +5 bp coding (23‑27/2850 nt) rcsC ← histidine kinase RcsC
RA 2,337,899 G→A L508L (CTG→TTG)  gyrA ← DNA gyrase subunit A
RA 2,343,289 C→T G294D (GGC→GAC)  yfaL ← putative autotransporter adhesin YfaL
RA 2,368,016 C→T P316S (CCA→TCA)  arnC → undecaprenyl‑phosphate 4‑deoxy‑4‑formamido‑L‑arabinose transferase
RA 2,369,820 C→T F594F (TTC→TTT arnA → fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase
RA 2,381,273 C→T V91M (GTG→ATG)  elaA ← putative acetyltransferase ElaA
RA 2,388,510 C→T intergenic (‑84/‑125) yfbN ← / → yfbO uncharacterized protein YfbN/uncharacterized protein YfbO
RA 2,397,444 G→A Q908* (CAA→TAA)  nuoG ← NADH:quinone oxidoreductase subunit G
JC JC 2,406,780 IS1 (–) +8 bp intergenic (‑139/‑774) lrhA ← / → alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
RA 2,436,027 C→T G208D (GGT→GAT)  usg ← putative semialdehyde dehydrogenase Usg
RA 2,445,454 C→T G36E (GGG→GAG)  yfcA ← putative transporter YfcA
RA 2,446,775 C→T A233A (GCG→GCA aroC ← chorismate synthase
RA 2,448,413 C→T V10I (GTA→ATA)  prmB ← ribosomal protein L3 N(5)‑glutamine methyltransferase
RA 2,453,265 C→T intergenic (‑929/+1818) yfcS ← / ← yfcV putative fimbrial chaperone YfcS/putative fimbrial protein YfcV
RA 2,487,809 C→T L1146L (CTG→TTG)  evgS → sensor histidine kinase EvgS
RA 2,520,359 A→C L105R (CTG→CGG)  gltX ← glutamate‑‑tRNA ligase
RA 2,521,000 C→T noncoding (70/76 nt) valU → tRNA‑Val
RA 2,522,135 C→T V115I (GTT→ATT)  xapR ← DNA‑binding transcriptional activator XapR
RA 2,551,843 C→T G99D (GGC→GAC)  ypeA ← putative acetyltransferase YpeA
RA 2,555,053 C→T A44T (GCT→ACT)  eutR ← putative DNA‑binding transcriptional regulator EutR
RA 2,555,270 C→T T153T (ACG→ACA eutK ← putative structural protein, ethanolamine utilization microcompartment
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,589,006 C→T S471F (TCT→TTT)  acrD → multidrug efflux pump RND permease AcrD
RA 2,601,372 C→T P58S (CCT→TCT)  hyfA → hydrogenase 4 component A
RA 2,603,648 C→T L611F (CTT→TTT)  hyfB → hydrogenase 4 component B
RA 2,609,197 C→T F237F (TTC→TTT hyfG → hydrogenase 4 catalytic subunit HyfG
RA 2,636,644 C→T R238H (CGT→CAT)  der ← 50S ribosomal subunit stability factor
RA 2,660,164 C→T K42K (AAG→AAA iscU ← scaffold protein for iron‑sulfur cluster assembly
JC JC 2,666,074 IS1 (+) +9 bp coding (640‑648/1281 nt) csiE → stationary phase‑inducible protein CsiE
RA 2,667,552 C→T V99M (GTG→ATG)  hcaT ← putative 3‑phenylpropionate transporter
RA 2,674,316 C→T D125N (GAT→AAT)  yphB ← putative aldose 1‑epimerase YphB
RA 2,675,265 C→T G166E (GGA→GAA)  yphC ← putative zinc‑binding dehydrogenase YphC
RA 2,686,052 C→T A73V (GCG→GTG)  hmp → nitric oxide dioxygenase
RA 2,699,001 C→T P81P (CCC→CCT yfhH → putative DNA‑binding transcriptional regulator YfhH
RA 2,701,959 C→T G171R (GGG→AGG)  recO ← recombination mediator protein RecO
RA 2,763,456 G→A L29L (CTG→TTG)  abpB ← putative helicase YfjK
RA 2,799,259 C→T F32F (TTC→TTT alaE → L‑alanine exporter
RA 2,830,641 C→T Q277* (CAA→TAA)  gutQ → D‑arabinose 5‑phosphate isomerase GutQ
RA 2,837,401 C→T V9I (GTC→ATC)  hypF ← carbamoyl‑‑[HypE] ligase
RA 2,858,185 C→T L365L (CTG→TTG)  mutS → DNA mismatch repair protein MutS
RA 2,864,638 C→T P135S (CCA→TCA)  ygbM → putative 2‑oxo‑tetronate isomerase YgbM
RA 2,880,559 C→T A164T (GCA→ACA)  casD ← type I‑E CRISPR system Cascade subunit CasD
RA 2,892,943 C→T S96F (TCC→TTC)  ygcN → putative oxidoreductase with FAD/NAD(P)‑binding domain
RA 2,914,023 C→T Q326Q (CAG→CAA rlmD ← 23S rRNA m(5)U1939 methyltransferase
RA 2,925,138 G→A A48V (GCC→GTC)  syd ← SecY‑interacting protein
RA 3,123,727 C→T G27R (GGA→AGA)  glcB ← malate synthase G
RA 3,222,234 G→A G257S (GGC→AGC)  ebgR → DNA‑binding transcriptional repressor EbgR
RA 3,228,994 G→A G36D (GGC→GAC)  ygjK → glycoside hydrolase
RA 3,257,869 C→T A48T (GCG→ACG)  cyuP ← cysteine detoxification protein CyuP
RA 3,268,970 A→G T186A (ACA→GCA)  yhaC → uncharacterized protein YhaC
RA 3,276,217 C→T R312R (CGC→CGT garD → GarD
RA 3,277,851 C→T intergenic (+50/+5) yhaV → / ← agaR ribosome‑dependent mRNA interferase toxin YhaV/DNA‑binding transcriptional repressor AgaR
RA 3,325,701 G→A A412V (GCT→GTT)  ftsH ← ATP‑dependent zinc metalloprotease FtsH
RA 3,358,472 G→A G1250S (GGC→AGC)  gltB → glutamate synthase subunit GltB
RA 3,403,891 G→A M136I (ATG→ATA yhdH → acrylyl‑CoA reductase
RA 3,484,496 G→A A3T (GCC→ACC)  prkB → putative phosphoribulokinase
RA 3,486,205 C→A T29K (ACG→AAG)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
MC JC 3,682,261 Δ34 bp coding (1154‑1187/1287 nt) dctA ← C4 dicarboxylate/orotate:H(+) symporter
JC JC 3,737,137 IS2 (+) +5 bp coding (37‑41/825 nt) bax ← putative glycoside hydrolase Bax
RA 3,832,198 A→G intergenic (+32/+21) yicH → / ← yicI AsmA family protein YicH/alpha‑D‑xyloside xylohydrolase
RA 3,885,012 C→T P66L (CCT→CTT)  yidD → membrane protein insertion efficiency factor
RA 3,941,942 C→T noncoding (135/1542 nt) rrsC → 16S ribosomal RNA
RA 3,941,982 C→T noncoding (175/1542 nt) rrsC → 16S ribosomal RNA
RA 3,952,440 C→T T80T (ACC→ACT ilvM → acetolactate synthase II subunit IlvM
RA 3,954,014 C→T S179S (TCC→TCT ilvD → dihydroxy‑acid dehydratase
RA 3,956,719 C→T F463F (TTC→TTT ilvA → threonine deaminase
RA 3,956,756 C→T L476L (CTG→TTG)  ilvA → threonine deaminase
RA 3,969,001 C→T S362S (AGC→AGT rfe → UDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase
RA 3,979,472 C→T A291V (GCT→GTT)  wzyE → enterobacterial common antigen polymerase
RA 3,981,902 C→T S339F (TCT→TTT)  yifK → putative transporter YifK
RA 3,992,961 C→T N603N (AAC→AAT cyaA → adenylate cyclase
RA 4,013,020 C→T intergenic (‑204/‑33) metR ← / → metE DNA‑binding transcriptional dual regulator MetR/cobalamin‑independent homocysteine transmethylase
RA 4,045,760 C→T K281K (AAG→AAA yihG ← 1‑acylglycerol‑3‑phosphate O‑acyltransferase YihG
RA 4,063,945 C→T W117* (TGG→TGA ompL ← putative outer membrane porin L
RA 4,070,052 C→T S117N (AGC→AAC)  yihR ← putative sulfoquinovose mutarotase YihR
RA 4,084,913 C→T A304T (GCC→ACC)  fdoG ← formate dehydrogenase O subunit alpha
RA 4,093,692 C→T G194D (GGC→GAC)  rhaD ← rhamnulose‑1‑phosphate aldolase
RA 4,099,392 C→T T249T (ACC→ACT rhaR → DNA‑binding transcriptional activator RhaR
RA 4,108,716 C→T intergenic (+202/‑118) pfkA → / → sbp 6‑phosphofructokinase 1/sulfate/thiosulfate ABC transporter periplasmic binding protein Sbp
JC JC 4,123,923 IS2 (–) +5 bp coding (530‑534/1026 nt) cytR ← DNA‑binding transcriptional repressor CytR
RA 4,126,657 C→T A52T (GCC→ACC)  priA ← primosomal protein N'
JC JC 4,135,180 IS2 (–) +5 bp coding (1346‑1350/2181 nt) katG → catalase/hydroperoxidase HPI
RA 4,138,560 C→T G159D (GGC→GAC)  gldA ← L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
RA 4,146,389 (T)6→7 coding (132/879 nt) pflC → putative pyruvate formate‑lyase 2 activating enzyme PflC
RA 4,185,676 C→T S109S (TCC→TCT rpoC → RNA polymerase subunit beta'
RA 4,267,582 G→A G157S (GGC→AGC)  tyrB → tyrosine aminotransferase
RA 4,287,391 G→A intergenic (‑20/‑220) acs ← / → ytcB acetyl‑CoA synthetase (AMP‑forming)/protein YtcB
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,314,691 G→A P138S (CCC→TCC)  phnP ← 5‑phospho‑alpha‑D‑ribosyl 1,2‑cyclic phosphate phosphodiesterase
RA 4,326,289 G→A intergenic (‑548/+110) yjdN ← / ← yjdM PF06983 family protein YjdN/zinc ribbon domain‑containing protein YjdM
RA 4,331,783 G→A A428T (GCG→ACG)  proP → osmolyte:H(+) symporter ProP
RA 4,359,027 G→A I335I (ATC→ATT cadB ← lysine:cadaverine antiporter
RA 4,404,993 G→A A103T (GCA→ACA)  purA → adenylosuccinate synthetase
RA 4,411,028 C→T T159I (ACC→ATC)  yjfJ → PspA family protein YjfJ
RA 4,438,289 G→A E195K (GAA→AAA)  ytfI → protein YtfI
JC JC 4,536,236 IS5 (+) +4 bp intergenic (‑2183/‑358) topAI ← / → ythB toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB
RA 4,542,683 Δ1 bp intergenic (+50/‑432) fimE → / → fimA regulator for fimA/type 1 fimbriae major subunit
RA 4,581,278 A→G V62A (GTT→GCT)  hsdS ← type I restriction enzyme EcoKI specificity subunit
RA 4,589,116 G→A intergenic (‑37/+13) yjiX ← / ← btsT PF04328 family protein YjiX/pyruvate:H(+) symporter
RA 4,618,148 G→A intergenic (+46/‑81) deoC → / → deoA deoxyribose‑phosphate aldolase/thymidine phosphorylase
RA 4,623,009 C→T P414L (CCG→CTG)  yjjJ → toxin YjjJ

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196350 1203479 7130 4 [2] [2] 3 [icd]–[ymfL] [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,[ymfL]
* * ÷ NC_000913 1203721 1211421 7701 3 [1] [2] 3 [ymfL]–mcrA [ymfL],ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1978495 1979270–1978503 9–776 20 [0] [0] 20 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3423851–3424231 3424449–3424238 8–599 3 [2] [2] 3 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 381260 =NA (NA)26 (0.940) 24/490 0.3 NA noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 382183 NA (NA)noncoding (924/1331 nt) IS2 repeat region
* ? NC_000913 382179 =NA (NA)26 (0.950) 22/486 0.3 NA noncoding (920/1331 nt) IS2 repeat region
?NC_000913 = 382590 NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 = 56677436 (1.300)12 (0.440) 6/486 1.9 30.9% pseudogene (91/93 nt) renD protein RenD
?NC_000913 568036 = 18 (0.660)pseudogene (2/213 nt) renD protein RenD
* ? NC_000913 = 1397238NA (NA)20 (0.720) 20/490 0.4 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1979271 =0 (0.000)20 (0.720) 19/490 0.5 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL