Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,445,454 C→T G36E (GGG→GAG)  yfcA ← putative transporter YfcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,445,4540CT94.7% 49.8 / ‑6.0 19G36E (GGG→GAG) yfcAputative transporter YfcA
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (13/5);  total (13/6)
Fisher's exact test for biased strand distribution p-value = 3.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

GAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCGCCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTTCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGCCTGATGGCGG  >  NC_000913/2445215‑2445700
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      
gAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGCATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGGGTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGACGGAAGGGAGAGTAACtccccgccaccg                                                                                                                                                                                                                                             >  2:28131/1‑251 (MQ=255)
                              cctaaaCGTTTTGCCCCAGCATTGCGCCGCTAATGGAACCGACAAAGGTAATGGCGATATTGAGTTTCTGATCTCTTAATCTACCCCCTTTGGGGCGAATAAAGTAGATATTAGCGGAAATAGATCCGCCGCAGGCTTGCAGTTTATTGTTTGCCAGCGCATTAGCGGGAGACACCCCCGCTGCCATCAAGGCCGGAAGGGTGAGTACCTCACCGCCACCGGCAATCGAGTCGATAAATCCCGCCAGCATg                                                                                                                                                                                                               <  1:151472/247‑1 (MQ=255)
                                 gaccGTATTGCACCAGAGGTGCGCCCCTCATTGGGCCAACAAGGGTAAGGGGAATATTGAGTTTTTGATCGCTTAAACAACCCCCTTGGGGGCGAATAAAGAAGATAGTAGCGAAAAAAGGGCCGCGGCAGGCTTGCAGTTTATTGTTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATAAATGCCGGAATGGTGAGTAACTCACCGCCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGc                                                                                                                                                                                                             <  2:250013/247‑1 (MQ=255)
                                       aTTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACTCACCGCCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACaaa                                                                                                                                                                                                       >  1:193078/1‑250 (MQ=255)
                                                                      gACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACTCACCGCCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAAccc                                                                                                                                                                       >  1:248340/1‑250 (MQ=255)
                                                                                                  tGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACTCACCGCCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCAtt                                                                                                                                             >  1:199146/1‑249 (MQ=255)
                                                                                                          tAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGGGAGTAACTAACCGCAACCGGCAATCGAGTCGATAAATCCCGCAAGCATGGCGACAAAAAAGAGAACCCCCAGCAACAGCGGGGAAACAATAAACAGGATATTAAACGTTTCAATTAGATCACGTg                                                                                                                                   >  2:52086/1‑251 (MQ=255)
                                                                                                             acaaccctcTTTGCGGCGAATAAAGGAGATTGGGGGGGAAATAGGGCCGCGGCAGGCTTGCAGTTTATTGGTGGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACTCACCGCCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTc                                                                                                                                <  2:248340/243‑1 (MQ=255)
                                                                                                                   cACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACTCACCGCCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATcc                                                                                                                            >  1:4972/1‑249 (MQ=255)
                                                                                                                                              agCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACTCACCGCCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACgg                                                                                               >  2:66393/1‑251 (MQ=255)
                                                                                                                                                  gAAATAGACCCGCGGCAGGCTAGCAGTTTATTGGTTGACACCGCACTAGCGGGAGACATCCCCGCTGCCATCCATGCCGGCATGGTGAGTAACTCACAGCCACCGGCAATCGAGTCGATAAATCCCGGCAGCCTGGCGAAAAAAAAGCGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAAATCACGCGCTCATCCAGAAGCGACAGGCAGGAAGGCGGCACCGGAGGc                                                                                           >  2:61093/1‑251 (MQ=255)
                                                                                                                                                                         cAGTTTATGGGTGGCCAGCGAATTGGCGGGCGACTCCCCCGTGGCCTCAAATGCCGGAATGGTGAGTAACCCACCGCCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTTCTCAGGCTttgt                                                                    <  2:83286/251‑1 (MQ=255)
                                                                                                                                                                               aTTGTTTGCCAGCGCATGAGCGGGAGACATCCCCGCGGCCATCAATCCCGGAATGGTGATTAACTCCCCGCCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTTCTCAGGCTTTGTTGTTc                                                               <  2:9252/250‑1 (MQ=255)
                                                                                                                                                                                     tGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACTCACCGCCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTTCTCAGGCTTTGTTGTTCCCGGttt                                                        >  2:248933/1‑251 (MQ=255)
                                                                                                                                                                                            gcATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACTCACCGCCACCGGCAATCGAGTCCATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTTCTCAGGCTATGTTGTTCCCGGTTTTGGAGGt                                                 >  1:189477/1‑251 (MQ=255)
                                                                                                                                                                                                       gcacacccccGCTGCAATCAATGCCGGAATGGTGAGTACCTCACCGCCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCAGTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTTCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCt                                      <  2:227245/245‑1 (MQ=255)
                                                                                                                                                                                                                  cTGCCATCAATGCCGGAATGGTGAGTAACTCACCGCCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTTCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTg                           >  1:257521/1‑251 (MQ=255)
                                                                                                                                                                                                                     ccATCAATGCCGGAATGGTGAGTAACTCACCGCCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAAAGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTATTCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGccc                        >  2:22558/1‑251 (MQ=255)
                                                                                                                                                                                                                                  gAATGGTGAGTAACTCACCGCCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTTCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGc            >  1:106440/1‑251 (MQ=255)
                                                                                                                                                                                                                                            tAACTCACCGCCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTTCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGCCTGATGGCgg  >  1:59691/1‑251 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      
GAATGGGCAAAATCTGCCGTAAGACATCAGCCTGAACGTATTGCACCAGCAGTGCGCCGCTCATTGAGCCGACAAAGGTCATGGCGATATTGAGTTTCTGATCGCTTAAACTAACCACTTTGCGGCGAATAAAGTAGATAGTAGCGGAAATAGAGCCGCCGCAGGCTTGCAGTTTATTGGTTGCCAGCGCATTAGCGGGAGACATCCCCGCTGCCATCAATGCCGGAATGGTGAGTAACCCACCGCCACCGGCAATCGAGTCGATAAATCCCGCCAGCATGGCGACAAAAAAGAGAACTCCCAGCAACAGCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTTCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGCCTGATGGCGG  >  NC_000913/2445215‑2445700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: