Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,436,027	C→T	G208D (GGT→GAT)	usg ←	putative semialdehyde dehydrogenase Usg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,436,027	0	C	T	100.0%	154.6 / NA	43	G208D (GGT→GAT)	usg	putative semialdehyde dehydrogenase Usg
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/19);  total (24/19)

GGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTACCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGG  >  NC_000913/2435781‑2436262                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            ggcgTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTc                                                                                                                                                                                                                                         <  2:165456/251‑1 (MQ=255)      aCCCGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTAt                                                                                                                                                                                                                                    <  1:280962/251‑1 (MQ=255)           aaGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGc                                                                                                                                                                                                                               >  1:13952/1‑251 (MQ=255)                      acctGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGgc                                                                                                                                                                                                                      <  2:227220/249‑1 (MQ=255)                              tGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTg                                                                                                                                                                                                             <  2:232618/250‑1 (MQ=255)                              tGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTg                                                                                                                                                                                                             <  2:207795/250‑1 (MQ=255)                                ggAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATAGCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAAc                                                                                                                                                                                                          <  1:143961/251‑1 (MQ=255)                                  aaTTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGc                                                                                                                                                                                                        >  1:47290/1‑251 (MQ=255)                                                        caATATCTTCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAAtct                                                                                                                                                                                  >  1:106532/1‑251 (MQ=255)                                                            atCTTCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATcttctt                                                                                                                                                                              >  1:110003/1‑251 (MQ=255)                                                              cTTCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGcc                                                                                                                                                                                             <  2:55614/234‑1 (MQ=255)                                                              cTTCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGcc                                                                                                                                                                                             >  1:55614/1‑234 (MQ=255)                                                                 cGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTc                                                                                                                                                                          >  1:149043/1‑250 (MQ=255)                                                                  gCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGAAGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGAGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATATTATTCGTCa                                                                                                                                                                         >  1:64931/1‑250 (MQ=255)                                                                    cTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATc                                                                                                                                                                      >  1:256372/1‑251 (MQ=255)                                                                        aaCAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGGGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGCCGCCCGAAGAAATCTTCTTCGTCAATCGGa                                                                                                                                                                   >  1:248054/1‑250 (MQ=255)                                                                            aaCGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGcc                                                                                                                                                                                                       >  2:46088/1‑210 (MQ=255)                                                                            aaCGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGcc                                                                                                                                                                                                       <  1:46088/210‑1 (MQ=255)                                                                                 aTCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTg                                                                                                                                                          >  2:215819/1‑250 (MQ=255)                                                                                                                        aaaGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGTACGTCGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTTCGTACTTCGTCAACGATACTCCGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGTCCGCtt                                                                                                                  <  1:65853/251‑1 (MQ=255)                                                                                                                               aCCATCTGGGCATGACCGTAGAATAGCGGTGCCTGGACGACGCTAGTCGGCATCATCAGACCTTACTCCTGCAGGATTTTGTGTACATCGTCAACCATAGGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGAAGCATGTTGAACGCCAGCTGACGCCCGAAGACATCTTCTTCGACAATCGGAATGCCGTTGAGCAATTTCGCACCCTGCCCCGCTAAAGCATCGACCGCTTTTTTGcc                                                                                                           >  2:108821/1‑251 (MQ=255)                                                                                                                                ccATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCActct                                                                                                                                          <  2:146630/219‑1 (MQ=255)                                                                                                                                ccATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCActct                                                                                                                                          >  1:146630/1‑219 (MQ=255)                                                                                                                                  aTCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTg                                                                                                        >  1:67684/1‑251 (MQ=255)                                                                                                                                      gggCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTggg                                                                                                      <  2:47290/249‑1 (MQ=255)                                                                                                                                         cATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAg                                                                                                 <  2:164586/251‑1 (MQ=255)                                                                                                                                                  aGAATTCCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTGTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCGTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATc                                                                                        <  2:711/251‑1 (MQ=255)                                                                                                                                                               cTGGACGACACCAGCCGAAATCATCAGCCCTTCGTCCTGCAGGCTTTTGCGTAATTCGTCAACGATCCGACGTTCTCCAAGCACGCTATCTTCGCTATCCGCCAGTAACCGCAGCATGTTGAACGCCAGCTGACGCCCGCAGCAATCTTATTCGTCCATCGGAATGCCGTTGACCCATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGACGGATGCTGAAATCAGCCTGGTAActc                                                                           >  2:134178/1‑249 (MQ=255)                                                                                                                                                                tGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCt                                                                          >  2:43411/1‑251 (MQ=255)                                                                                                                                                                    cgacgCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGAt                                                                       >  2:99154/1‑250 (MQ=255)                                                                                                                                                                     gacgCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATAc                                                                     <  1:206359/251‑1 (MQ=255)                                                                                                                                                                     gacgCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATAc                                                                     <  2:67684/251‑1 (MQ=255)                                                                                                                                                                     gacgCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATAc                                                                     <  1:206372/251‑1 (MQ=255)                                                                                                                                                                                      tcaGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTg                                                     >  1:8109/1‑250 (MQ=255)                                                                                                                                                                                          cccTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTTGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGa                                                <  1:16985/251‑1 (MQ=255)                                                                                                                                                                                                       aGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATGAACGGTTTc                                     >  1:238343/1‑249 (MQ=255)                                                                                                                                                                                                          aTTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTgc                                >  1:138680/1‑251 (MQ=255)                                                                                                                                                                                                           ttttGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTgcc                               >  1:265843/1‑251 (MQ=255)                                                                                                                                                                                                              tGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTgcc                               >  2:27748/1‑248 (MQ=255)                                                                                                                                                                                                              tGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTgcc                               <  1:27748/248‑1 (MQ=255)                                                                                                                                                                                                                           aaCGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGctggntgg               >  2:259260/1‑251 (MQ=255)                                                                                                                                                                                                                            aCGATACGACGTTCTTCACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGt              >  2:149438/1‑251 (MQ=255)                                                                                                                                                                                                                                           tcACGCACGCTATCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTgg  <  1:169572/248‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            GGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTACCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCTTCGTCAATCGGAATGCCGTTGAGCAATTTCGCACTCTGCCCCGCTAACGCATCGACCGCTTTTTTGCCCTGGGCGGAGGCTGAAATCAGGCTGGTAACGCTGATACGTGATAAACCGCCCTGATCGATTAACGGTTTCAGTGCCGCCAGCAGCTGGCTGGTCAGACTGTCTGG  >  NC_000913/2435781‑2436262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑