Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,914,023	C→T	Q326Q (CAG→CAA)	rlmD ←	23S rRNA m(5)U1939 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,914,023	0	C	T	100.0%	85.5 / NA	26	Q326Q (CAG→CAA)	rlmD	23S rRNA m(5)U1939 methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/13);  total (13/13)

ATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGGATACATAAACTATACGAATAGGTTCCAGTTTTATAATTTGCTGCATAACACCTGCGGCACCTGCTCGCGCCGGGTCCAGCAACACTTTATCGAAGCCGTTTTTCGCCCACGGCTGCTTTGTGACATCTTCTTCAAGATTTTCGTGATAAAACGTCACATTCTGTAAGCCATTAAGACGCGCATTCTGCTGGCCTTTTTCCACCAGCGCCGGAACACCTTCTACACCGACCACACTGGCAGCTTGTGTCGCCAATGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTCACACCCGCATTGACCTGAATAAAATCGCGCGGGCTAAAAGTTAAGCGCAACCCGTTTGAGTCATACCAGGGCA  >  NC_000913/2913783‑2914264                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  atatCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGGATACATAAACTATACGAATAGGTTCCAGTTTTATAATTTGCTGCATAACACCTGCGGCACCTGCTCGCGCCGGGTCCAGCAACACTTTATCGAAGCCGTTTTTCGCCCACGGCTGCTTTGTGACATCTTCTTCAAGATTTTCGTGATAAAACGTCACATTCTGTAAGCCATTAAGACGCGCATTTTGCTGGCCtt                                                                                                                                                                                                                                         <  1:276374/251‑1 (MQ=255)   atCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGGATACATAAACTATACGAATAGGTTCCAGTTTTATAATTTGCTGCATAACACCTGCGGCACCTGCTCGCGCCGGGTCCAGCAACACTTTATCGAAGCCGTTTTTCGCCCACGGCTGCTTTGTGACATCTTCTTCAAGATTTTCGTGATAAAACGTCACATTCTGTAAGCCATTAAGACGCGCATTTTGCTGGCCtttt                                                                                                                                                                                                                                       >  1:282127/1‑251 (MQ=255)           tttAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGGATACATAAACTATACGAATAGGTTCCAGTTTTATAATTTGCTGCATAACACCTGCGGCACCTGCTCGCGCCGGGTCCAGCAACACTTTATCGAAGCCGTTTTTCGCCCACGGCTGCTTTGTGACATCTTCTTCAAGATTTTCGTGATAAAACGTCACATTCTGTAAGCCATTAAGACGCGCATTTTGCTGGCCTTTTTccacca                                                                                                                                                                                                                                <  2:218890/250‑1 (MQ=255)           tttAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGGATACATAAACTATACGAATAGGTTCCAGTTTTATAATTTGCTGCATAACACCTGCGGCACCTGCTCGCGCCGGGTCCAGCAACACTTTATCGAAGCCGTTTTTCGCCCACGGCTGCTTTGTGACATCTTCTTCAAGATTTTCGTGATAAAACGTCACATTCTGTAAGCCATTAAGACGCGCATTTTGCTGGCCTTTTTccacca                                                                                                                                                                                                                                <  1:60689/250‑1 (MQ=255)                 aaCGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGGATACATAAACTATACGAATAGGTTCCAGTTTTATAATTTGCTGCATAACACCTGCGGCACCTGCTCGCGCCGGGTCCAGCAACACTTTATCGAAGCCGTTTTTCGCCCACGGCTGCTTTGTGACATCTTCTTCAAGATTTTCGTGATAAAACGTCACATTCTGTAAGCCATTAAGACGGGCATTTTGCTGGCCTTTTTCCACCAGCGCCgg                                                                                                                                                                                                                         >  2:85351/1‑251 (MQ=255)                                         gTTGCAGGGTTACAGGATACATAAACTATACGTATAGGTTCCAGTTTTATAATTTGCTGCATAACACCTGCGGCACCTGCTCGCGCCGGGTCCAGCAACACTTTATCGAAGCCGTTTTTCGCCCACGGCTGCTTTGTGACATCTTCTTCAAGAGTTTCGTGATAAAACGTCCCATTCTGTAAGCCATTAAGACGCGCATTTTGCTGGCCTTTTTCCACCAGCGCCGGAACACCTTCTACACCGACcactct                                                                                                                                                                                                 <  2:80971/251‑4 (MQ=255)                                                       gtaTACATAAACTATACGAATAGGTTCCAGTTTGATAATTTGCTGCATAACTCCTGCGGCTGCTGCGCGCGCCGGGTCCCGCAACACGTTATCGAAGCCGTTTTTCGCCCACGGCTGCGTTGTGACATCTTCTTCAAGATTTTCGTGATAAAACGTCACATTCTTTAAGCACTGAAGACCCGCATTTTGCTTGCCGTTTTCCCCCAGCGCCCGAACACCTTCTACACCGTCCACACTGTCAGCTTGGGTCg                                                                                                                                                                                   <  2:115388/249‑1 (MQ=255)                                                         aTACATAAACTATACGAATAGGCTCCAGTTTTATAATTTGCTGCAAAACAAATGCGGCACATGCTCGCGCCGGGTCCAGCAACACTTTATCGAAGCCGTTTTTCGCCCACGGATGCTTTGTGACATCTTCTTCAAGATTTTCGTGATAAAACGTCACACTCTGTCAGCCATTAAGACGCGCATTTTGCTGGCCTTTTTCCACCAGCGCCGGAACACCTTCTACAACGACCACACTGGAAGCTTGTGTCGcc                                                                                                                                                                                 >  1:28227/1‑251 (MQ=255)                                                                 aCTATCCGAATAGGTTCCAGTTTTATAATTTGCTGCATAACACCTGCGGCACCTGCTCGCGCCGGGTCCAGCAACACTTTATCGAAGCCGTTTTTCGCCCACGGCTGCTTTGTGACATCTTCTTCAAGATTTTCGTGATAAAACGTCACATTCTGTAAGCCATTAAGACGCGCATTTTGCTGGCCTTTTTCCACCAGCGCCGGAACACCTTCTACACCGACCACACTGGCAGCTTGTGTCGCCAATGGc                                                                                                                                                                           >  2:11525/1‑249 (MQ=255)                                                                                         ataATTTGCTGCATAACACCTGCGGCACCTGCTCGCGCCGGGTCCAGCAACACTTTACCGAAGCCGTTTTTCGCCCACGGCTGCTTTGTGACATCTTCTTCAAGATTTTCGTGATAAAACGTCACATTCTGTAAGCCATTAAGACGCGCATTTTGCTGGCCTTTTTCCACCAGCGCCGGAACACCTTCTACACCGACCACACTGGCAGCTTGTGTCGCCAATGGCAGTGTAAAGTTGCCCATACCGCAGaa                                                                                                                                                 <  2:69777/251‑1 (MQ=255)                                                                                            aTTTGCTGCATAACACCTGCGGCACCTGCTCGCGCCGGGTCCAGCAACACTTTATCGAAGCCGTTTTTCGCCCACGGCTGCTTTGTGACATCTTCTTCAAGATTTTCGTGATAAAACGTCACATTCTGTAAGCCATTAAGACGCGCATTTTGCTGGCCTTTTTCCACCAGCGCCGGAACACCTTCTACACCGACCACACTGGCAGCTTGTGTCGCCAATGGCAGTGTAAAGTTGCCCATACCGCAGAAc                                                                                                                                                >  2:90748/1‑249 (MQ=255)                                                                                                         cacCTGCGGCACCTGCTCGCGCCGGGTCCAGCAACTCTTTATCGAAGCCGTTTTTCGCCCACGGCTGCTTTGTGACATCTTCTTCAAGATTTTCGTGATAAAACGTCACATTCTGTAAGCCATTAAGTCGCGGATTTTGCTGGCCTTTTTCCACCAGCGCCGTAACACCTGCTACTCCGACCACACTGGCAGCTTGTGTCGCCAATGGCATTGTAAAGTTGCCCGTACCGCAGAACAGATCCAGTACGCGa                                                                                                                                 <  2:30087/251‑1 (MQ=255)                                                                                                                                                 aTCGAAGCCGTTTTTCGCCCACGGCTGCTTTGTGACATCTTCTTCAAGATTTTCGTGATAAAACGTCACATTCTGTAAGCCATTAAGACGCGCATTTTGCTGGCCTTTTTCCACCAGCGCCGGAACACCTTCTACACCGACCACACTGGCAGCTTGTGTCGCCAATGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTCGCACATCCAGCCATTCCAACGCACGCGCTa                                                                                         <  1:8716/251‑1 (MQ=255)                                                                                                                                                               tCGCCCACGGCTGCTTTGTGACATCTTCTTCAAGATTTTCGTGATAAAACGTCACATTCTGTAAGCCATTAAGCCGCGCATTTTGCTGGCCTTTTTCCACCAGCGCCGGAACCCCTTCTACACCGCCCACACTGGCAGCTTGTGTCGCCAATGGCAGTGTAAAGTTGCCCATACCGCAGAACACATCCAGTTCGCGATCTTCAGGTTGCACATCCAGCCATTCCTACGCCCGCGCTACCATTTTTTGGtt                                                                            <  2:61932/250‑1 (MQ=255)                                                                                                                                                                   ccACGGCTGCTTTGTGACATCTTCTTCAAGATTTTCGTGATAAAACGTCACCTTCTGTCAGCCATTAAGACGCGCATTTTGCTGGCCTTTTTCCACCAGCGCCGGACCACCTTCTACACCGACCACACTGGCAGCTTGTGTCGCCAATGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGCTCTTAAGGTTGCACATCCAGCCATTCCAACGCACGCGATACCACTTTTTGGTTCacac                                                                       >  2:30764/1‑251 (MQ=255)                                                                                                                                                                       ggCTGCTTTGTGACATCTTCTTCAAGATTTTCGTGATAAAACGTCACATTCTGTAAGCCATTAAGACGCGCATTTTGCTGGCCTTTTTCCACCAGCGCCGGAACACCTTCTACACCGACCACACTGGCAGCTTGTGTCGCCAATGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTCACACCCGc                                                                   >  1:254706/1‑251 (MQ=255)                                                                                                                                                                           gctTTGTGACATCTTCTTCAAGATTTTCGTGATAAAACGTCACATTCTGTAAGCCATTAAGACGCGCATTTTGCTGGCCTTTTTCCACCAGCGCCGGAACACCTTCTACACCGACCACACTGGCAGCTTGTGTCGCCAATGGCCGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTCACACCCGCAttt                                                               >  2:174746/1‑250 (MQ=255)                                                                                                                                                                            ctTTGTGACATCTTCTTCAAGATTTTCGTGATAAAACGTCACATTCTGTAAGCCATTAAGACGCGCATTTTGCTGGCCTTTTTCCACCAGCGCCGGAACACCTTCTACACCGACCACACTGGCAGCTTGTGTCGCCAATGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTCACACCCGCANTg                                                               >  2:262005/1‑250 (MQ=255)                                                                                                                                                                                         tcttcAAGATTTTCGTGATAAAACGTCACATTCTGTAAGCCATTAAGACGCGCATTTTGCTGGCCTTTTTCCACCAGCGCCGGAACACCTTCTACACCGACCACACTGGCAGCTTGTGTCGCCAATGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTCACACCCGCATTGACCTGAATaa                                                     <  2:282127/247‑1 (MQ=255)                                                                                                                                                                                                gATTTTCGTGATAAAACGTCAAATTCTGTAAGCCATTAAGACGCGCATTTTGCTGGCCTTTTTCCACCAGCGCCGGAACACCTTCTACACCGACCACACTGGCAGCTTGTGTCGCCAATGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTCACACCCGCATTGACCTGAATAAAATCGCGCggg                                          >  1:214432/1‑251 (MQ=255)                                                                                                                                                                                                 aTTTTCGTGATAAAACGTCACATTCTGTAAGCCATTAAGACGCGCATTTTGCTGGCCTTTTTCCACCAGCGCCGGAACACCTTCTACACCGACCACACTGGCAGCTTGTGTCGCCAATGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTCACACCCGCATTGACCTGAATAAAATCGCGCGGGc                                         <  1:135517/251‑1 (MQ=255)                                                                                                                                                                                                        tGATAAAACGTCACATTCTGTAAGCCATTAAGACGCGCATTTTGCTGGCCTTTTTCCACCAGCGCCGGAACACCTTCTACACCGACCACACTGGCAGCTTGTGTCGCCAATGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTCACACCCGCATTGACCTGAATAAAATCGCACGGGCTAAAAGt                                  >  1:256304/1‑251 (MQ=255)                                                                                                                                                                                                          aTAAAACGTCACATTCTGTAAGCCATTAAGACGCGCATTTTGCTGGCCTTTTTCCACCAGCGCCGGAACACCTTCTACACCGACCACACTGGCAGCTTGTGTCGCCAATGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTCACACCCGCATTGACCTGAATAAAATCGCGCGGGCTAAAAGtt                                 >  2:42705/1‑250 (MQ=255)                                                                                                                                                                                                          aTAAAACGTCACACTATGTAAGCCATTAAGACGCACATTTTGATGGCCTTTTTCCACAAGCGCCGGAACACCTTCTACACCGACCACACTGGCAGCATGTGTCGCCAATGGCAGTGTAAAGTGGCCAATACAGCAGAACAGATCCAGAACGCGAACTTCAGGTTGCACATCCAGCCATTCGAACGCACGCGCTACCATTTTTTGGTTCACACCCGCATTGACCTGAATAAAATCTCGCGGGCTAAAAgat                                 >  1:80704/1‑248 (MQ=255)                                                                                                                                                                                                                           gTAAGCCATTAAGACGCGCATTTTGCTGGCCTTTTTCCACCAGCGCCGGAACACCTTCTACACCGACCACACTGGCAGCTTGTGTCGCCAATGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTCACACCCGCATTGACCTGAATAAAATCGCGCGGGCTAAAAGTTAAGCGCAACCCGttt                  <  1:24384/248‑1 (MQ=255)                                                                                                                                                                                                                                        ccgcgCATTTTACTGGCCTTTTTCCACCAGCGCCGGAACACCTTCTACACCGACCACACTGTCAGCTTGTGTCGCCAATGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTCACACCCGCATTGACCTGAATAAAATCGCGCGGGCTAAAAGTTAAGCGCAACCCGTTTGAGTCATACCAGGGCa  <  1:125776/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  ATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGGATACATAAACTATACGAATAGGTTCCAGTTTTATAATTTGCTGCATAACACCTGCGGCACCTGCTCGCGCCGGGTCCAGCAACACTTTATCGAAGCCGTTTTTCGCCCACGGCTGCTTTGTGACATCTTCTTCAAGATTTTCGTGATAAAACGTCACATTCTGTAAGCCATTAAGACGCGCATTCTGCTGGCCTTTTTCCACCAGCGCCGGAACACCTTCTACACCGACCACACTGGCAGCTTGTGTCGCCAATGGCAGTGTAAAGTTGCCCATACCGCAGAACAGATCCAGTACGCGATCTTCAGGTTGCACATCCAGCCATTCCAACGCACGCGCTACCATTTTTTGGTTCACACCCGCATTGACCTGAATAAAATCGCGCGGGCTAAAAGTTAAGCGCAACCCGTTTGAGTCATACCAGGGCA  >  NC_000913/2913783‑2914264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑